Ly-6B.2 Alloantigen antibody | 7/4
Rat anti Mouse Ly-6B.2 Alloantigen
- Product Type
- Monoclonal Antibody
- Clone
- 7/4
- Isotype
- IgG2a
- Specificity
- Ly-6B.2 Alloantigen
Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa (Rosas et al.2010). In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimura et al. 1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982). Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- MCA771GT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- MCA771GA, MCA771G: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Cultured bone marrow cells
- Approx. Protein Concentrations
- MCA771GT, MCA771G: IgG concentration 1.0 mg/ml
- MCA771GA: IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/50 | 1/100 | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Western Blotting |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Rat IgG2a Negative Control | MCA1212 | E F | 1 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Rat IgG2a Negative Control |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Antigen Retrieval Buffer, pH8.0 |
Source Reference
-
Hirsch, S. & Gordon, S. (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4.
Immunogenetics. 18 (3): 229-39.
References for Ly-6B.2 Alloantigen antibody
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- RRID
- AB_322950
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