Ly-6B.2 Alloantigen antibody | 7/4

Rat anti Mouse Ly-6B.2 Alloantigen

Product Type
Monoclonal Antibody
Clone
7/4
Isotype
IgG2a
Specificity
Ly-6B.2 Alloantigen

Product Code Applications Pack Size List Price Your Price Qty
MCA771G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF P WB 0.25 mg loader
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loader
MCA771GA
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SDS Safety Datasheet SDS
C F IF P WB 0.1 mg loader
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loader
MCA771GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF P WB 25 µg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa (Rosas et al.2010).

In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimura et al. 1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).

Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
MCA771G, MCA771GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
MCA771GT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Cultured bone marrow cells
Approx. Protein Concentrations
Pack Size: 0.25 mg, 25 µg
IgG concentration 1.0 mg/ml
Pack Size: 0.1 mg
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Source Reference

  1. Hirsch, S. & Gordon, S. (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4.
    Immunogenetics. 18 (3): 229-39.

References for Ly-6B.2 Alloantigen antibody

  1. Chen, C.J. et al. (2006) MyD88-dependent IL-1 receptor signaling is essential for gouty inflammation stimulated by monosodium urate crystals.
    J Clin Invest. 2006 Aug;116(8):2262-71.
  2. Chen, Z. et al. (2004) Evidence for a role of macrophage migration inhibitory factor in vascular disease.
    Arterioscler Thromb Vasc Biol. 24: 709-14.
  3. Cheng, C.W. et al. (2007) Quantitative cellular and molecular analysis of the effect of progesterone withdrawal in a murine model of decidualization.
    Biol Reprod. 76: 871-83.
  4. Clark, J.D. et al. (2007) Morphine reduces local cytokine expression and neutrophil infiltration after incision.
    Mol Pain. 3:28.
  5. Crockett, E.T. et al. (2004) Gene deletion of P-Selectin and ICAM-1 does not inhibit neutrophil infiltration into peritoneal cavity following cecal ligation-puncture.
    BMC Clin Pathol. 4: 2
  6. Day, Y.J. et al. (2004) Protection from ischemic liver injury by activation of A2A adenosine receptors during reperfusion: inhibition of chemokine induction.
    Am J Physiol Gastrointest Liver Physiol. 286: G285-93.
  7. Endlich, B. et al. (2002) Distinct temporal patterns of macrophage-inflammatory protein-2 and KC chemokine gene expression in surgical injury.
    J Immunol. 168: 3586-94.
  8. Hobbs, J.A. et al. (2003) Myeloid cell function in MRP-14 (S100A9) null mice.
    Mol Cell Biol. 23: 2564-76.
  9. View The Latest Product References
  10. Horiba, M. et al. (2000) Neointima formation in a restenosis model is suppressed in midkine-deficient mice.
    J Clin Invest. 105: 489-95.
  11. Iwaki, T. et al. (2005) A cardioprotective role for the endothelial protein C receptor in lipopolysaccharide-induced endotoxemia in the mouse.
    Blood. 105: 2364-71.
  12. Kato, N. et al. (2009) The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    J Am Soc Nephrol. 20: 1565-76.
  13. Kaur, S. et al. (2004) Acidic duodenal pH alters gene expression in the cystic fibrosis mouse pancreas.
    Am J Physiol Gastrointest Liver Physiol. 287: G480-90.
  14. Kim, M. et al. (2010) Selective renal overexpression of human heat shock protein 27 reduces renal ischemia-reperfusion injury in mice.
    Am J Physiol Renal Physiol. 299: F347-58.
  15. Lacroix-Lamandé, S. et al. (2009) Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation.
    PLoS One. 4: e8291.
  16. Lacroix-Lamandé, S. et al. (2002) Role of gamma interferon in chemokine expression in the ileum of mice and in a murine intestinal epithelial cell line after Cryptosporidium parvum infection.
    Infect Immun. 70: 2090-9.
  17. Lassen, S. et al. (2010) Ischemia reperfusion induces IFN regulatory factor 4 in renal dendritic cells, which suppresses postischemic inflammation and prevents acute renal failure
    J Immunol. 185: 1976-83.
  18. Laubitz, D. et al. (2008) Colonic gene expression profile in NHE3-deficient mice: evidence for spontaneous distal colitis.
    Am J Physiol Gastrointest Liver Physiol. 295: G63-G77.
  19. Lech, M. et al. (2009) Resident dendritic cells prevent postischemic acute renal failure by help of single Ig IL-1 receptor-related protein.
    J Immunol. 183: 4109-18.
  20. Lee, H.T. et al. (2004) A1 adenosine receptor activation inhibits inflammation, necrosis, and apoptosis after renal ischemia-reperfusion injury in mice.
    J Am Soc Nephrol. 15: 102-11.
  21. Liao, C. et al. (2001) Altered myelopoiesis and the development of acute myeloid leukemia in transgenic mice overexpressing cyclin A1.
    Proc Natl Acad Sci U S A. 98: 6853-8.
  22. Morison, N.B. et al. (2007) The long-term actions of etonogestrel and levonorgestrel on decidualized and non-decidualized endometrium in a mouse model mimic some effects of progestogen-only contraceptives in women.
    Reproduction. 133: 309-21.
  23. Omari, K.M. et al. (2009) Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1.
    Am J Pathol. 174: 164-76.
  24. Plüddemann, A. (2009) The macrophage scavenger receptor A is host-protective in experimental meningococcal septicaemia.
    PLoS Pathog. 5:e1000297.
  25. Pyo, R. et al. (2000) Targeted gene disruption of matrix metalloproteinase-9 (gelatinase B) suppresses development of experimental abdominal aortic aneurysms.
    J Clin Invest. 105: 1641-9.
  26. Sasaki, T. et al. (2006) A simple method of plaque rupture induction in apolipoprotein E-deficient mice.
    Arterioscler Thromb Vasc Biol. 26: 1304-9.
  27. Sato, J. et al. (2003) The fibrinolytic system in dissemination and matrix protein deposition during a mycobacterium infection.
    Am J Pathol. 163: 517-31.
  28. Schneider, D.J. et al. (2010) Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease.
    FASEB J. 24: 70-80.
  29. Shi, H. et al. (2010) MAP kinase phosphatase-1 deficiency impairs skeletal muscle regeneration and exacerbates muscular dystrophy.
    FASEB J. 24: 2985-97.
  30. Shornick, L.P. et al. (2008) Airway epithelial versus immune cell Stat1 function for innate defense against respiratory viral infection.
    J Immunol. 180: 3319-28.
  31. Singbartl, K. et al. (2001) Platelet, but not endothelial, P-selectin is critical for neutrophil-mediated acute postischemic renal failure.
    FASEB J. 15: 2337-44.
  32. Smalley-Freed, W.G. et al. (2010) p120-catenin is essential for maintenance of barrier function and intestinal homeostasis in mice.
    J Clin Invest. 120: 1824-35.
  33. Song, Y. et al. (2001) A low level of TNF-alpha mediates hemorrhage-induced acute lung injury via p55 TNF receptor.
    Am J Physiol Lung Cell Mol Physiol. 281: L677-84.
  34. Thatcher, T.H. et al. (2005) Role of CXCR2 in cigarette smoke-induced lung inflammation.
    Am J Physiol Lung Cell Mol Physiol. 289: L322-8.
  35. Timmons, B.C. & Mahendroo, M.S. (2006) Timing of neutrophil activation and expression of proinflammatory markers do not support a role for neutrophils in cervical ripening in the mouse.
    Biol Reprod. 74: 236-45.
  36. Tsou, C.L. et al. (2007) Critical roles for CCR2 and MCP-3 in monocyte mobilization from bone marrow and recruitment to inflammatory sites.
    J Clin Invest. 117: 902-9.
  37. Rosas, M. et al. (2010) The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B.
    J Leukoc Biol. 88 (1): 169-80.
  38. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues.
    Curr Top Microbiol Immunol. 181: 1-37.
  39. Word, R.A. et al. (2005) Transgene insertion on mouse chromosome 6 impairs function of the uterine cervix and causes failure of parturition.
    Biol Reprod. 73 (5): 1046-56.
  40. Locke, L.W. (2009) A novel neutrophil-specific PET imaging agent: cFLFLFK-PEG-64Cu.
    J Nucl Med. 50: 790-7.
  41. Allam, R. et al. (2011) Cyclic Polypeptide and Aminoglycoside Antibiotics Trigger IL-1{beta} Secretion by Activating the NLRP3 Inflammasome.
    J Immunol. 186: 2714-8.
  42. Holt, R. et al. (2011) The Molecular Mechanisms of Cervical Ripening Differ between Term and Preterm Birth.
    Endocrinology. 152: 1036-46.
  43. Frossard, J.L. et al. (2011) Role of CCL-2, CCR-2 and CCR-4 in cerulein-induced acute pancreatitis and pancreatitis-associated lung injury.
    J Clin Pathol. 64: 387-93.
  44. McDonald, J.U. et al. (2011) In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.
    FASEB J. 25: 1972-82.
  45. Larmonier, C.B. et al. (2011) NHE3 modulates the severity of colitis in IL-10-deficient mice.
    Am J Physiol Gastrointest Liver Physiol. 300: G998-G1009.
  46. Yellon, S.M. et al. (2011) Remodeling of the cervix and parturition in mice lacking the progesterone receptor B isoform.
    Biol Reprod. 85: 498-502.
  47. Nadeau, S. et al. (2011) Functional Recovery after Peripheral Nerve Injury is Dependent on the Pro-Inflammatory Cytokines IL-1{beta} and TNF: Implications for Neuropathic Pain.
    J Neurosci. 31: 12533-12542.
  48. Bombardelli, L. et al. (2010) Pancreas-specific ablation of beta1 integrin induces tissue degeneration by disrupting acinar cell polarity.
    Gastroenterology. 138: 2531-40,
  49. Guerriero, J.L. et al. (2011) DNA alkylating therapy induces tumor regression through an HMGB1-mediated activation of innate immunity.
    J Immunol. 186: 3517-26.
  50. Brulhart-Meynet, M.C. et al. (2015) Improving Reconstituted HDL Composition for Efficient Post-Ischemic Reduction of Ischemia Reperfusion Injury.
    PLoS One. 10 (3): e0119664.
  51. Patel, J. et al. (2015) RGS1 regulates myeloid cell accumulation in atherosclerosis and aortic aneurysm rupture through altered chemokine signalling.
    Nat Commun. 6: 6614.
  52. Hamers, A.A. et al. (2015) Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages.
    PLoS One. 10 (8): e0133598.
  53. Ao, M. et al. (2015) Dental Infection of Porphyromonas gingivalis Induces Preterm Birth in Mice.
    PLoS One. 10 (8): e0137249.
  54. Cnops, J. et al. (2015) NK-, NKT- and CD8-Derived IFNγ Drives Myeloid Cell Activation and Erythrophagocytosis, Resulting in Trypanosomosis-Associated Acute Anemia.
    PLoS Pathog. 11 (6): e1004964.
  55. Cardenas, H. et al. (2016) Dietary Apigenin Exerts Immune-Regulatory Activity in Vivo by Reducing NF-κB Activity, Halting Leukocyte Infiltration and Restoring Normal Metabolic Function.
    Int J Mol Sci. 17 (3). pii: E323. doi: 10.3390/ijms17030323.
  56. Park, S.W. et al. (2009) Human activated protein C attenuates both hepatic and renal injury caused by hepatic ischemia and reperfusion injury in mice.
    Kidney Int. 76 (7): 739-50.
  57. Boal, F. et al. (2016) PI5P Triggers ICAM-1 Degradation in Shigella Infected Cells, Thus Dampening Immune Cell Recruitment.
    Cell Rep. 14 (4): 750-9.
  58. Nguyen, H.T. & Shen, H. (2016) The effect of PEGylation on the stimulation of IL-1β by gold (Au) nanoshell/silica core nanoparticles.
    J Mater Chem B Mater Biol Med. 4 (9): 1650-1659.
  59. Choi, E. et al. (2015) Expression of Activated Ras in Gastric Chief Cells of Mice Leads to the Full Spectrum of Metaplastic Lineage Transitions.
    Gastroenterology. Dec 8. pii: S0016-5085(15)01735-7. [Epub ahead of print]
  60. Zhao, Y. et al. (2016) Rapamycin prevents bronchiolitis obliterans through increasing infiltration of regulatory B cells in a murine tracheal transplantation model.
    J Thorac Cardiovasc Surg. 151 (2): 487-496.e3.
  61. Wan, W. et al. (2015) Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice.
    Cardiovasc Res. 106 (3): 478-87.
  62. Wieser, V. et al. (2016) Lipocalin 2 drives neutrophilic inflammation in alcoholic liver disease.
    J Hepatol. 64 (4): 872-80.
  63. Kidoya, H. et al. (2015) APJ Regulates Parallel Alignment of Arteries and Veins in the Skin.
    Dev Cell. 33 (3): 247-59.
  64. Kjellman, P. et al. (2015) Size-dependent lymphatic uptake of nanoscale-tailored particles as tumor mass increases.
    Future Sci OA. 1 (4): FSO60.
  65. Brennan, F.H. et al. (2015) The Complement Receptor C5aR Controls Acute Inflammation and Astrogliosis following Spinal Cord Injury.
    J Neurosci. 35 (16): 6517-31.
  66. Farrar, C.A. et al. (2016) Collectin-11 detects stress-induced L-fucose pattern to trigger renal epithelial injury.
    J Clin Invest. Apr 18. pii: 83000. [Epub ahead of print]
  67. Laubitz, D. et al. (2016) Reduced Epithelial Na+/H+ Exchange Drives Gut Microbial Dysbiosis and Promotes Inflammatory Response in T Cell-Mediated Murine Colitis.
    PLoS One. 11 (4): e0152044.
  68. Rabadi, M.M. et al. (2016) Peptidyl arginine deiminase-4 deficient mice are protected against kidney and liver injury after renal ischemia and reperfusion.
    Am J Physiol Renal Physiol. Jun 22: ajprenal.00254.2016. [Epub ahead of print]
  69. Morris, A. H. et al. (2016) Inadequate Processing of Decellularized Dermal Matrix Reduces Cell Viability In Vitro and Increases Apoptosis and Acute Inflammation In Vivo.
    BioResearch Open Access. 5 (1): 177-87.
  70. Ni, H.M. et al. (2016) Caspase Inhibition Prevents Tumor Necrosis Factor-α-Induced Apoptosis and Promotes Necrotic Cell Death in Mouse Hepatocytes in Vivo and in Vitro.
    Am J Pathol. Sep 7. pii: S0002-9440(16)30236-X. [Epub ahead of print]
  71. Wang, S. et al. (2016) Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury.
    Oncotarget. 7 (14): 17681-98.
  72. Konrad, F.M. et al. (2019) How Adhesion Molecule Patterns Change While Neutrophils Traffic through the Lung during Inflammation.
    Mediators Inflamm. 2019: 1208086.
  73. Jung, P.E. et al. (2020) Honokiol Protects the Kidney from Renal Ischemia and Reperfusion Injury by Upregulating the Glutathione Biosynthetic Enzymes.
    Biomedicines. 8 (9): 352.
  74. Chatterjee, S. et al. (2013) Junctional adhesion molecule-A regulates vascular endothelial growth factor receptor-2 signaling-dependent mouse corneal wound healing.
    PLoS One. 8 (5): e63674.
  75. Han, Y. et al. (2020) Close Homolog of L1 Deficiency Exacerbated Intestinal Epithelial Barrier Function in Mouse Model of Dextran Sulfate Sodium-Induced Colitis.
    Front Physiol. 11: 584508.
  76. Leinweber, J. et al. (2021) Elastase inhibitor agaphelin protects from acute ischemic stroke in mice by reducing thrombosis, blood-brain barrier damage, and inflammation.
    Brain Behav Immun. S0889-1591(20)32485-5.
  77. Tilstra, J.S. et al. (2020) B cell-intrinsic TLR9 expression is protective in murine lupus.
    J Clin Invest. 130 (6): 3172-3187.
  78. Gordon, R.A. et al. (2020) Murine lupus is neutrophil elastase-independent in the MRL.Faslpr model.
    PLoS One. 15 (4): e0226396.
  79. Matsumoto, K. et al. (2021) Juvenile social defeat stress exposure favors in later onset of irritable bowel syndrome-like symptoms in male mice.
    Sci Rep. 11 (1): 16276.
  80. Hawkins, R.B. et al. (2021) Mesenchymal Stem Cells Alter MicroRNA Expression and Attenuate Thoracic Aortic Aneurysm Formation.
    J Surg Res. 268: 221-231.
  81. Chute, M. et al. (2022) ADAM15 is required for optimal collagen cross-linking and scar formation following myocardial infarction.
    Matrix Biol. 105: 127-43.
  82. Beuker, C. et al. (2022) Stroke induces disease-specific myeloid cells in the brain parenchyma and pia.
    Nat Commun. 13 (1): 945.
  83. Zheng, Z. et al. (2022) Role of TRPC6 in kidney damage after acute ischemic kidney injury.
    Sci Rep. 12 (1): 3038.
  84. Filiberto, A.C. et al. (2022) Endothelial pannexin-1 channels modulate macrophage and smooth muscle cell activation in abdominal aortic aneurysm formation.
    Nat Commun. 13 (1): 1521.

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

RRID
AB_322950
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