Ly-6B.2 Alloantigen antibody | 7/4

Rat anti Mouse Ly-6B.2 Alloantigen

Product Type
Monoclonal Antibody
Ly-6B.2 Alloantigen

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF P WB 0.25 mg loader
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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF P WB 0.1 mg loader
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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF P WB 25 µg loader
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Search for Batch Specific Datasheets

Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa (Rosas et al.2010).

In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimura et al. 1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).

Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010).

Target Species
Product Form
Purified IgG - liquid
MCA771G, MCA771GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
MCA771GT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Cultured bone marrow cells
Approx. Protein Concentrations
Pack Size: 0.25 mg, 25 µg
IgG concentration 1.0 mg/ml
Pack Size: 0.1 mg
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.
For research purposes only
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Source Reference

  1. Hirsch, S. & Gordon, S. (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4.
    Immunogenetics. 18 (3): 229-39.

References for Ly-6B.2 Alloantigen antibody

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Flow Cytometry


Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

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