Ly-6B.2 Alloantigen antibody | 7/4
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunohistochemistry.
Image caption:
Effect of atorvastatin on platelet and neutrophil infiltration after reperfusion. The infiltration of platelets (upper panel) and neutrophils (lower panel) were evaluated by immunohistochemistry in the 4 chimeric groups that received 40 min of LAD occlusion and 60 min of reperfusion. Platelets and neutrophils were found predominantly in the ischemic area in B6/B6 chimeras. Atorvastatin reduced both platelet and neutrophil infiltration in B6/B6 and B6/KO chimeras, but not in KO/B6 chimeras.
From: Tian Y, Linden J, French BA, Yang Z (2014) Atorvastatin at Reperfusion Reduces Myocardial Infarct Size in Mice by Activating eNOS in Bone Marrow-Derived Cells. PLoS ONE 9(12): e114375.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunofluorescence.
Image caption:
CX3CR1+, Ly6Clo monocytes are present in perihematomal brain in CX3CR1-null BM chimeras. Mice were injected intracardially with 150 ug tomato lectin to delineate vasculature. Brain slices were stained with Ly6B.2 antibody to differentiate Ly6Chi monocytes and neutrophils from Ly6Clo CX3CR1+ monocytes and Dapi nuclear stain. A) Intracardiac injection of lectin stains vasculature in the brain– 20x. B) At 72 hours after ICH, distinct subsets of amoeboid Ly6B.2+ (Ly6Chi monocytes or neutrophils) and GFP+ (CX3CR1+) monocytes are seen in the perihematomal region. 20x. C) At 7 days after ICH, amoeboid, Ly6B.2−,CX3CR1+ cells are seen in the brain parenchyma surrounding the ICH cavity. The center of the ICH cavity is filled with Ly6B.2+ (Ly6Chi) monocytes or neutrophils. Tomato lectin staining is also seen in the center of the ICH cavity, most likely due to blood brain barrier breakdown at this time point leading to nonspecific lectin staining of myeloid cells. 10x. White box indicates the inset shown in panel E. D) At 14 days, amoeboid, CX3CR1+ cells can still be seen in the perihematomal region, however more CX3CR1+ cells have a ramified morphology. No Ly6B.2+ (Ly6Chi monocytes or neutrophils) cells are found. Overall blood-brain barrier disruption is improved leading to less lectin staining in the cavity, although there remain some lectin+ cells (CX3CR1+ monocytes and CX3CR1− microglia). 10x. White box indicates inset shown in panel F. E) 20x image of panel C – perihematomal region 7 days after ICH F) 20x image of panel D – perihematomal region 14 days after ICH. Blue – Dapi, GFP – CX3CR1, Red – Tomato lectin, Pink – Ly6B.2, n = 5.
From: Taylor RA, Hammond MD, Ai Y, Sansing LH (2014) CX3CR1 Signaling on Monocytes Is Dispensable after Intracerebral Hemorrhage. PLoS ONE 9(12): e114472.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunohistochemistry.
Image caption:
Post-ischemic treatment with HDL does not decrease leukocyte infiltration. Mice were submitted to LAD occlusion for 45min and hearts were reperfused for 24h. Mice were injected or not (control mice IR) with native HDL (nHDL), rHDLB (apoAI + POPC + S1P) one minute before reperfusion. A. Quantification of infiltrated neutrophils (Ly-6B.2+ cells) per area in frozen sections of infarcted hearts at 24h of reperfusion. B. Representative images of neutrophil (Ly-6B.2+ cells) infiltration. C. Quantification of infiltrated neutrophils (Ly6G+ cells) per area in frozen sections of infarcted hearts at 24h of reperfusion. D. Representative images of neutrophil (Ly6G+ cell) infiltration. E. Quantification of infiltrated macrophages (CD68+ cells) per area in frozen sections of infarcted hearts at 24h of reperfusion. F. Representative images of macrophage (CD68+ cells) infiltration. Data are expressed as scattered plots (mean±SEM, n = 7–10 per group). Results are expressed as percentages of stained area on total heart surface area. No significance difference between groups was found using unpaired-student t-test.
From: Brulhart-Meynet M-C, Braunersreuther V, Brinck J, Montecucco F, Prost J-C, et al. (2015) Improving Reconstituted HDL Composition for Efficient Post-Ischemic Reduction of Ischemia Reperfusion Injury. PLoS ONE 10(3): e0119664.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of neutrophils in mouse abdominal aorta by immunogluorescence.
Image caption:
Flow cytometric analysis of bead-labelled aortic leukocytes in ApoE−/− and Rgs1−/− ApoE−/− mice that received Ang II infusion at 0.8 mg kg−1 per day for 5 days following fluorescent bead labelling of circulating inflammatory monocytes. (a) Representative dot plots shown for gated aortic cells of each positive population from ApoE−/− and Rgs1−/− ApoE−/− mice with representative percentages. Labels on both axes are on a log scale. (b) Quantification of the number of bead-labelled CD45+ cells in aortas of Ang II-infused mice at days 3 and 5. (c) Immunofluorescence microscopy of abdominal aortas from ApoE−/− mice at day 5 after Ang II infusion and bead labelling stained for Ly6C and 7/4 (red), 4′,6-diamidino-2-phenylindole (blue). Arrows indicate the presence of cells containing fluorescent beads (green) on the luminal side (L) of the aorta or within the internal elastic laminar (green autofluorescence). Adv, adventitia. **P<0.01 calculated using the Student’s t-test (n=5–7 per group. Data in b are expressed as mean±s.e.m.).
From: Patel J, McNeill E, Douglas G, Hale AB, de Bono J, Lee R, Iqbal AJ, Regan-Komito D, Stylianou E, Greaves DR, Channon KM. RGS1 regulates myeloid cell accumulation in atherosclerosis and aortic aneurysm rupture through altered chemokine signalling. Nat Commun. 2015 Mar 18;6:6614.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of infiltrating neutrophils in a murine colitis model by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Nur77-/- mice develop augmented DSS-induced colitis. The body weight of the mice was determined daily during DSS treatment (A). Upon harvest colon weight, spleen weight (B) and colon length (C) were determined. The disease activity index (DAI) was calculated as an average in weight loss, stool consistency and bleeding scores (D). Colon histology was scored on hematoxillin-eosin stained sections (E) for the parameters shown (F). Neutrophil numbers were counted in healthy and DSS colon sections and the influx was calculated by taking the ratio of Healthy / DSS (G). Representative photomicrographs are shown with original magnification 100x. Colon pieces were cultured overnight and cytokine levels were determined in the supernatant and corrected for mg of tissue (H). Data are presented as mean±SEM; *p<0.05, **p<0.01, ***p<0.001. Scale bars represent 1mm. ME = Muscularis externa, KC = Chemokine ligand 1 (mouse homologue of IL-8; a potent neutrophil attractant).
From: Hamers AAJ, van Dam L, Teixeira Duarte JM, Vos M, Marinković G, van Tiel CM, et al. (2015) Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages.
PLoS ONE 10(8): e0133598.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Increased Inflammatory Cell Infiltration and Inflammatory Mediators in Placental Tissues Following P.g. Infection. Immunohistochemical staining of gd 15 placental tissues of NC and P.g.-infected groups (n = 6 for each group). (A,B) PMNLs, (C,D) F4/80 positive macrophages, (E,F) TNF-α positive macrophages, (G, H) COX-2 positive staining, (I, J) CD-31 stained endothelial cells, (K,L) 8OHdG (oxidative DNA stress marker), (M,N) cleaved-caspase 3 (apoptosis marker), and (O,P) histomorphometric analysis of PMNLs and F4/80 (macrophages). Scale bars, 100μm. Statistical significance was determined using the Student t-test. *p<0.05, **p<0.01. Olympus BH2 microscope and Nikon digital sight DS-L2 camera were used for capturing images. (Q) Representative results of mRNA expression of COX-2, Gal-3, IL-8 and TNF-α in placental tissues in NC group and P.g.-infected group (n = 3 for each), GAPDH was used as internal control. Molecular weights were labeled to the right of each band. Yellow dashed square shows that PMNLs are restricted to the blood vessels.
From: Ao M, Miyauchi M, Furusho H, Inubushi T, Kitagawa M, Nagasaki A, et al. (2015) Dental Infection of Porphyromonas gingivalis Induces Preterm Birth in Mice.
PLoS ONE 10(8): e0137249.
Ly-6B.2 Alloantigen antibody, clone 7/4 used for the evaluation of Ly6B.2 expression on various leukocyte types by flow cytometry.
Image caption:
A) First, CD45+ cells were selected based on a FSC-A/CD45 profile followed by gating on single cells (SSC-A/FSC-W profile). Then, CD11b+ cells were selected using an CD11b/FSC-A profile within the CD45+ population. Subsequently, neutrophils (CD11b+Ly6cintLy6G+) were identified using a Ly6G/FSC-A profile and the remaining cells were used in an Ly6C versus MHC-II profile to identify monocytes (CD11b+Ly6chighLy6G-MHC-II-), monocyte-derived macrophages (CD11b+Ly6chighLy6G-MHC-II+), resident macrophages (CD11b+Ly6c-Ly6G-MHC-II-) and a Rest fraction (CD11b+Ly6c-Ly6G-MHC-II-). B) Surface marker expression on different myeloid cell subsets. Expression of F4/80, CCR2, Ly6B, MerTK and CD64 is displayed. C. Myeloid cell composition of liver in absolute cell numbers. D. Myeloid cell composition of spleen in absolute cell numbers. Values represent mean ± SD of 4 mice per group. *: p-value < 0.05 and if nothing is mentioned the differences were not significant.
From: Citation: Cnops J, De Trez C, Stijlemans B, Keirsse J, Kauffmann F, Barkhuizen M, et al. (2015) NK-, NKT- and CD8-Derived IFNγ Drives Myeloid Cell Activation and Erythrophagocytosis, Resulting in Trypanosomosis-Associated Acute Anemia.
PLoS Pathog 11(6): e1004964.
Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 used for the detection of inflammatory neutrophils in mouse lung tissue sections by immunofluorescence
Image caption:
Apigenin reduces LPS-induced apoptosis in the lungs. Lungs from mice treated with LPS, control, apigenin (Api) or apigenin 3 h prior to LPS (Api + LPS) were obtained at 24 h. (A) The number of apoptotic cells was determined by the TUNEL assay and expressed as the mean ± SEM of the numbers of stained cells per 1000× microscopic field (cpf) (N = 4 mice for each biological condition; * p < 0.01 vs. LPS), scale bars: 25 μm for all figures; (B,C) Caspase-3 activity was determined by the DEVD-AFC assay using lung lysates as described in materials and methods. Values represent the means ± SEM (N = 4 mice for each biological condition; * p < 0.05 vs. LPS); (D) Lung tissue immuno-histochemistry using antibodies specific for active caspase-3 in combination with anti-CD31, anti-cytokeratin or anti-7/4 antibodies specific for endothelia, epithelia and neutrophils, respectively. Scale bars, 100 μm.
From: Cardenas, H.; Arango, D.; Nicholas, C.; Duarte, S.; Nuovo, G.J.; He, W.; Voss, O.H.; Gonzalez-Mejia, M.E.; Guttridge, D.C.; Grotewold, E.; Doseff, A.I.
Dietary Apigenin Exerts Immune-Regulatory Activity in Vivo by Reducing NF-κB Activity, Halting Leukocyte Infiltration and Restoring Normal Metabolic Function.
Int. J. Mol. Sci. 2016, 17, 323.
Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 used for the identification of infiltrating neutrophils in murine pulmonary tissue sections by immunohistochemistry.
Image caption:
Apigenin decreases LPS-induced neutrophil infiltration in the lungs. Lungs from mice receiving Api + LPS, LPS, Api or vehicle (control) were collected at 24 h post-challenge. (A) H&E staining; (B) Chloroacetate esterase (CAE) staining; (C) Lungs stained with anti-7/4 antibodies; (D) Leukocyte infiltration determined by counting CAE-positive cells per high power field (cpf) in lungs used in (B); (E) Number of infiltrated neutrophils as determined by counting stained cells per cpf in in lungs used in (C). Data represent the mean ± SEM (N = 9 mice for each biological condition; * p < 0.05 vs. LPS). Scale bars for all figures are 25 μm and 100 μm for insets.
From: Cardenas, H.; Arango, D.; Nicholas, C.; Duarte, S.; Nuovo, G.J.; He, W.; Voss, O.H.; Gonzalez-Mejia, M.E.; Guttridge, D.C.; Grotewold, E.; Doseff, A.I.
Dietary Apigenin Exerts Immune-Regulatory Activity in Vivo by Reducing NF-κB Activity, Halting Leukocyte Infiltration and Restoring Normal Metabolic Function.
Int. J. Mol. Sci. 2016, 17, 323.
Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 (MCA771G) used for the detection of neutrophils in murine kidney tissue sections by immunohistochemistry. Macrophages were detected using Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497 )
Image caption:
Immunostaining of macrophages and neutrophils in the kidneys of M-Rac1 FC and KO mice. (A) Representative micrographs of F4/80 immunostaining in the kidneys of Vehicle- or LPS-injected M-Rac1 FC and KO mice. Original magnification x 200. (B) Quantitative analysis of F4/80-positive macrophages. Data are means ± s.e.m. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 5 per each group. (C) Representative micrographs of Ly-6B.2 (mAb 7/4) immunostaining. (D) Quantitative analysis of Ly-6B.2-positive neutrophils. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 6 per group.
From: Nagase M, Kurihara H, Aiba A, Young MJ, Sakai T (2016)
Deletion of Rac1GTPase in the Myeloid Lineage Protects against Inflammation-Mediated Kidney Injury in Mice.
PLoS ONE 11(3): e0150886.
Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 used to identify neutrophils in formalin fixed, paraffin embedded murine placental tissue sections.
Image caption:
Increased Inflammatory Cell Infiltration and Inflammatory Mediators in Placental Tissues Following P.g. Infection.Immunohistochemical staining of gd 15 placental tissues of NC and P.g.-infected groups (n = 6 for each group). (A,B) PMNLs, (C,D) F4/80 positive macrophages, (E,F) TNF-α positive macrophages, (G, H) COX-2 positive staining, (I, J) CD-31 stained endothelial cells, (K,L) 8OHdG (oxidative DNA stress marker), (M,N) cleaved-caspase 3 (apoptosis marker), and (O,P) histomorphometric analysis of PMNLs and F4/80 (macrophages). Scale bars, 100μm. Statistical significance was determined using the Student t-test. *p 7lt; 0.05, **p < 0.01. Olympus BH2 microscope and Nikon digital sight DS-L2 camera were used for capturing images. (Q) Representative results of mRNA expression of COX-2, Gal-3, IL-8 and TNF-α in placental tissues in NC group and P.g.-infected group (n = 3 for each), GAPDH was used as internal control. Molecular weights were labeled to the right of each band. Yellow dashed square shows that PMNLs are restricted to the blood vessels.
From: Ao M, Miyauchi M, Furusho H, Inubushi T, Kitagawa M, Nagasaki A, et al. (2015)
Dental Infection of Porphyromonas gingivalis Induces Preterm Birth in Mice.
PLoS ONE 10(8): e0137249.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the identification ofneutrophils in the distal colonic mucosa by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Increased T cell and neutrophil infiltration in the colonic mucosa of DKO/AT mice is accompanied by altered MLN Treg phenotype.
(A and B) Representative images of immunohistochemical analysis of (A) CD4+ cell abundance and (B) neutrophil abundance in the distal colonic mucosa; (C) qPCR analysis of mucosal expression of neutrophil collagenase MMP8; (D) Flow cytometry analysis of regulatory T cells in mesenteric lymph nodes (MLN) in adoptively transferred mice and effect of antibiotics. Bar graphs represent mean with SD values. Statistical analysis were performed using one-way ANOVA (P value indicated in each panel) with Fisher's LSD post-hoc test. Asterisks in all panels indicate statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
From: Laubitz D, Harrison CA, Midura-Kiela MT, Ramalingam R, Larmonier CB, Chase JH, et al. (2016)
Reduced Epithelial Na+/H+ Exchange Drives Gut Microbial Dysbiosis and Promotes Inflammatory Response in T Cell-Mediated Murine Colitis.
PLoS ONE 11(4): e0152044.
Rat anti Mouse LY-6B.2 antibody, clone 7/4 (MCA771G) used for detection of neutrophils in murine skin implants by immunohistochemistry.
Image caption:
Sample d-TT (A) and d-TT EW (B) were implanted subcutaneously for 3 days before excision and immunohistochemical analysis. d-TT showed increased Ly-6B-positive cells (neutrophils) compared to d-TT EW (scale bar is 50 μm). Quantification of staining (C) shows increases in Ly-6B in d-TT. *p <0.05.
From: Morris Aaron H., Chang Julie, and Kyriakides Themis R.
BioResearch Open Access. July 2016, 5(1): 177-87.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA771G) used for the identification of neutrophils in an alchohol induced model of liver injury by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
RIP3 KO mice decrease the hepatic expression of inflammatory cytokine genes but have no effects on Gao-binge alcohol-induced neutrophil infiltration
WT and RIP3 KO mice were treated with the Gao-binge model. A. RNA from mouse livers was used to measure gene expression by qPCR. Results were normalized to β-actin and expressed as fold change compared to WT control. Data shown are means ± S.E (n = 3-4 mice per group. *p<0.05; by one-way ANOVA). B. Representative images were shown from Ly6B immunohistochemistry after Gao-binge treatment. Arrows denote the Ly6B positive neutrophils. C. The number of Ly6B positive neutrophils was counted from at least 22 areas (400 X magnification) each mouse. Results shown are means ± S.E. (n = 4-6 mice per group. **p<0.01 compared to WT control by one-way ANOVA).
From: Wang S, Ni HM, Dorko K, Kumer SC, Schmitt TM, Nawabi A, Komatsu M, Huang H, Ding WX.
Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury.
Oncotarget. 2016 Apr 5;7(14):17681-98.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA771GA) used for the detection of Ly-6B.2 positive cells by immunofluorescence.
Image caption:
Confocal neutrophil-cytokine double labeling experiments using incised skin. For these studies skin from the wound edges was harvested 2 hours after incision. After sectioning, the tissue was exposed to anti-neutrophil and anti-IL-1β antibodies followed by the application of CY3 (green fluorescence, neutrophils, panel A) and FITC (red, IL-1β, panel B) conjugated secondary antibodies. Panel C presents the merged image with arrows pointing to several strongly double labeling cells. The scale bar in panel C is 50 μm in length. These micrographs were taken of dermal tissue under 630× magnification.
From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA771GA) used for the detection of Ly-6B.2 positive cells by flow cytometry.
Image caption:
Characterization of high dose zymosan peritonitis. A) Representative flow-cytometric density plots showing the recruitment of inflammatory cells and the clearance of fluorescent (FITC)-labelled zymosan particles. The examples shown are taken from 18 hours after the administration of zymosan. Cells were identified as described in the methods. The panel on the left shows the gating of Ly-6B+ cells, which includes (right) Ly-6Ghigh neutrophils and Ly-6G− monocytes and MØ. Both neutrophils and monocytes/MØ exhibit association with FITC-labeled zymosan at this time point (right panel). B) Graphical representation of the number of neutrophils (left) and all types of monocyte (Mo) and MØ combined (right) in the peritoneal cavity before and after acute zymosan peritonitis. n = 3 129S6/SvEv mice per group and is representative of 2 independent experiments. Data is shown as mean±SEM and cells were isolated from naïve animals (0 hours), and challenged mice 4, 18, 72 and 168 hours after induction of peritonitis. C) Graphical representation of the percentage of neutrophils and Mo/MØ present that are associated with zymosan, scored by flow-cytometry as indicated in (A) above. D) Photomicrograph, which is representative of neutrophils associated with zymosan in a 4 hour inflammatory infiltrate. Those neutrophils that are associated tend to have multiple particles, but only represent a minority of the total neutrophils present at this time (see (C) above).
From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012)
Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation.
PLoS ONE 7(9): e45781.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA771GA) used for the detection of Ly-6B.2 positive cells by immunohistochemistry.
Image caption:
Peri-incisional infiltration of neutrophils, immunohistochemical appearance. Hind paw skin from incised mice was processed for the identification of neutrophils. Panel A displays an H&E stained section of plantar hind paw skin demonstrating a predominantly dermal cellular infiltrate 2 hours after incision. The dermal and epidermal layers are labeled. Panel B displays the appearance of non-incised hind paw skin stained with a neutrophil-specific antibody. Panel C displays a micrograph taken of incised skin stained for neutrophils 2 hours after incision. Note the abundance of darkly staining infiltrating neutrophils in the dermis of this section compared to that displayed in panel B. The scale bar in panel C is 150 μm in length. All micrographs were taken using 200× magnification.
From: Clark JD, Shi X, Li X, Qiao Y, Liang D, Angst MS, Yeomans DC. Morphine reduces local cytokine expression and neutrophil infiltration after incision. Mol Pain. 2007 Oct 2;3:28.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA771GA) used for the detection of Ly-6B.2 positive cells by immunohistochemistry.
Image caption:
Renal inflammatory changes in Ntn1+/− mice following ischemia. Ntn1+/− mice and their respective age-, weight-, and gender-matched littermate controls (Ntn1+/+) were subjected to 30 minutes of left renal artery ischemia. (A–D) Neutrophil staining. Arrows indicate neutrophils (magnification 400×). (E) Quantification of neutrophil tissue accumulation by measurement of myeloperoxidase (MPO). (F) TNF-α and (G) interleukin-6 (IL-6) and (H) interleukin-10 (IL-10) were assessed by real-time RT-PCR from renal tissues. Data were calculated relative to β-actin and are expressed as fold change compared to sham-operated animals without ischemia (−I). Data are representative of four to six independent experiments for each experimental condition (mean ± SD).
From: Grenz A, Dalton JH, Bauerle JD, Badulak A, Ridyard D, et al. (2011) Partial Netrin-1 Deficiency Aggravates Acute Kidney Injury. PLoS ONE 6(5): e14812.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA771GA) used for the detection of Ly-6B.2 positive cells by immunofluorescence.
Image caption:
Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 μg/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistology. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.
From: Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, et al. (2009)
Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation.
PLoS ONE 4(12): e8291.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA771GA) used for the detection of Ly-6B.2 positive cells by immunofluorescence.
Image caption:
166 mM H2O2 increased neutrophil infiltration in day 1 and 6 wounds. Fluorescence intensity of the neodermis was quantified using ImageJ. The area quantified is outlined with the dashed line. Results shown are mean ± S.E.M, n = 6-7. A representative section from each treatment is shown. ES – Eschar; HE – Hyper-proliferating epidermis; ND – neodermis.*p<0.05.
From: Loo AEK, Wong YT, Ho R, Wasser M, Du T, et al. (2012) Effects of Hydrogen Peroxide on Wound Healing in Mice in Relation to Oxidative Damage.
PLoS ONE 7(11): e49215
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA771GA) used for the detection of Ly-6B.2 positive cells by flow cytometry.
Image caption:
F4/80 and CD11b+ cells infiltrating the iris after the intracameral injection of antigen also express Ly6C, 7/4 (Ly 6B) and CD45. Preparations of naïve iris cells and cells recovered after an intracameral injection of antigen were stained with anti- F4/80 and (A) Ly6C and 7/4 (B) show a characteristic CD45 peak C) Ly6Glo or negative, CD115, CD49b+, CD62Llo. The figures are representative of 2 experiments. All the isotype controls in the histograms are shown as shaded.
From: Pais R, Bhowmick S, Chattopadhyay S, Lemire Y, Sharafieh R, et al. (2012) An Intracameral Injection of Antigen Induces In Situ Chemokines and Cytokines Required for the Generation of Circulating Immunoregulatory Monocytes. PLoS ONE 7(8): e43182.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 (MCA771GA) used for the detection of Ly-6B.2 positive cells by immunohistochemistry.
Image caption:
Intact spleen segments from infected animals were fixed in paraformaldehyde, embedded in OCT, sectioned, and stained with various antibodies for the infiltration of macrophages (FA-11), polymorphonuclear neutrophils, and activated monocytes (7/4Ag) and activated macrophages (MHC-II), as described in the text. All data are representative of at least three individual experiments.
From: Plüddemann A, Hoe JC, Makepeace K, Moxon ER, Gordon S (2009) The Macrophage Scavenger Receptor A Is Host-Protective in Experimental Meningococcal Septicaemia.
PLoS Pathog 5(2): e1000297.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunofluorescence.
Image caption:
Neutrophils infiltrate p120 null regions of the small intestine and colon. (A) p120 KO small intestine and colon sections were co-stained for p120 and neutrophils. Arrows and arrowheads indicate regions of p120 ablation. (B) Neutrophils were quantified in oil and tamoxifen-induced small intestine (p = .06) and colon (p = .0092) samples. (C) At the two-month time point, mice were gavaged with FITC-dextran, and serum levels of FITC-dextran were measured to test for an intestinal permeability defect. FITC-dextran levels were significantly higher (3.3-fold) in p120 KO mice (p = .03).
From: Smalley-Freed WG, Efimov A, Short SP, Jia P, Zhao Z, et al. (2011) Adenoma Formation following Limited Ablation of p120-Catenin in the Mouse Intestine. PLoS ONE 6(5): e19880.
Rat anti Mouse Ly-6B.2 antibody, clone 7/4 used for the detection of Ly-6B.2 positive cells by immunohistochemistry.
Image caption:
Changes in markers of hepatic inflammation in wild type and hepatocyte-specific HIF-1β knockout mice after Gao-binge treatment. Age matched male wild type and hepatocyte-specific HIF-1β knockout mice were subjected to Gao-binge treatment. Hepatic mRNA was isolated, and real-time RT-PCR was performed as described in the Materials and Methods (A). Data are presented as means ± SE (n = 3–5). Liver tissue was subjected to immunostaining for neutrophils using an anti-Ly6B antibody and representative images are shown in (B, 40x), and the number of neutrophils were quantified from 10 different fields of each mouse. (C). Data are presented as average number of neutrophils in each filed (means ± SE; n = 4).
From: Ni H-M, Bhakta A, Wang S, Li Z, Manley S, et al. (2014) Role of Hypoxia Inducing Factor-1β in Alcohol-Induced Autophagy, Steatosis and Liver Injury in Mice. PLoS ONE 9(12): e115849.
Rat anti Mouse Ly-6B.2 Alloantigen:Alexa Fluor® 647
Rat anti Mouse Ly-6B.2 Alloantigen:Biotin
Rat anti Mouse Ly-6B.2 Alloantigen:FITC
Rat anti Mouse Ly-6B.2 Alloantigen:Low Endotoxin
Rat anti Mouse Ly-6B.2 Alloantigen
Rat anti Mouse Ly-6B.2 Alloantigen:RPE
Rat anti Mouse Ly-6B.2 Alloantigen:Alexa Fluor® 700
- Product Type
- Monoclonal Antibody
- Clone
- 7/4
- Isotype
- IgG2a
Rat anti Mouse Ly-6B.2 monoclonal antibody, clone 7/4 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa (Rosas et al.2010).
In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimura et al. 1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).
Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010).
In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains (Kimura et al. 1984). Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity (Hirsch and Gordon 1982).
Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilisedPack Size: 100 Tests, 500 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Reconstitution
- Pack Size: 25 Tests/0.25mlReconstitute with 0.25 ml distilled waterPack Size: 100 TestsReconstitute with 1ml distilled waterPack Size: 500 TestsReconstitute with 5.0ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- Pack Size: 25 µg0.09% Sodium Azide (NaN3)
1% Bovine Serum AlbuminPack Size: 0.1 mg0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Cultured bone marrow cells
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- Pack Size: 25 µg, 50 µg, 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Pack Size: 25 µg, 0.25 mgIgG concentration 1.0 mg/mlPack Size: 0.1 mgIgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 µg, 50 µg, 0.1 mgStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 Tests/0.25mlPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 Tests, 500 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
- Shelf Life
- 18 months from date of despatch.
More Information
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of Ly-6B.2 Alloantigen antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat Pack Size: 25 µg, 0.1 mg 1/50 Pack Size: 0.5 mg |
1/10 Pack Size: 25 µg, 0.1 mg 1/100 Pack Size: 0.5 mg |
|
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting | |||
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting | |||
| Flow Cytometry | Neat | Neat Pack Size: 25 Tests/0.25ml, 100 Tests 1/10 Pack Size: 500 Tests |
|
| Flow Cytometry | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | 100 Tests/1ml | F | ||
| Rat IgG2a Negative Control:FITC | MCA1212F | 100 Tests | F | ||
| Rat IgG2a Negative Control:Low Endotoxin | MCA1212EL | 0.5 mg | F | ||
| Rat IgG2a Negative Control | MCA1212 | 1 ml | E F | ||
| Rat IgG2a Negative Control:RPE | MCA1212PE | 100 Tests | F | ||
| Rat IgG2a Negative Control:Alexa Fluor® 700 | MCA1212A700 | 100 Tests/1ml | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F |
Application Based External Images
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Product Specific References
Source Reference
-
Hirsch, S. & Gordon, S. (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4.
Immunogenetics. 18 (3): 229-39.
References for Ly-6B.2 Alloantigen antibody
-
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Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues.
Curr Top Microbiol Immunol. 181: 1-37. -
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