Ly-6A/E antibody | D7
|Rat anti Mouse Ly-6A/E antibody, clone D7 recognizes members of the lymphocyte activation protein-6 (Ly-6) multi-gene family. Specifically, it recognizes Ly-6A.2 and Ly-6E.1. Ly-6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly-6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells.
Rat anti Mouse Ly-6A/E antibody, clone D7 has been reported to induce T cell activation. Removal of sodium azide is recommended prior to use in functional assays.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared from ascites or tissue culture supernatant
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- IL-2-dependent mouse T cell line CTL-L.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
References for Ly-6A/E antibody
Holmes, C. & Stanford, W.L. (2007) Concise review: stem cell antigen-1: expression, function, and enigma.
Stem Cells. 25 (6): 1339-47.
Ortega, G. et al. (1986) Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity.
J Immunol. 137 (10): 3240-6.
Malek, T.R. et al. (1986) Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies.
J Exp Med. 164 (3): 709-22.
Codias, E.K. et al. (1990) Down-regulation of IL-2 production by activation of T cells through Ly-6A/E.
J Immunol. 145 (5): 1407-14.
Snapper, C.M. et al. (1991) Cross-linkage of Ly-6A/E induces Ca2+ translocation in the absence of phosphatidylinositol turnover and mediates proliferation of normal murine B lymphocytes.
J Immunol. 147 (4): 1171-9.
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