Ly-6A/E antibody | D7

Rat anti Mouse Ly-6A/E

Product Type
Monoclonal Antibody
Clone
D7
Isotype
IgG2a
Specificity
Ly-6A/E

Product Code Applications Pack Size List Price Your Price Qty
MCA2782
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP WB 0.25 mg
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Search for Batch Specific Datasheets

Rat anti Mouse Ly-6A/E antibody, clone D7 recognizes members of the lymphocyte activation protein-6 (Ly-6) multi-gene family. Specifically, it recognizes Ly-6A.2 and Ly-6E.1. Ly-6A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly-6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells.

Rat anti Mouse Ly-6A/E antibody, clone D7 has been reported to induce T cell activation. Removal of sodium azide is recommended prior to use in functional assays.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared from ascites or tissue culture supernatant
Buffer Solution
Borate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
IL-2-dependent mouse T cell line CTL-L.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description Rabbit F(ab')2 anti Rat IgG:FITC
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
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loader
Description Rat IgG2a Negative Control

References for Ly-6A/E antibody

  1. Holmes, C. & Stanford, W.L. (2007) Concise review: stem cell antigen-1: expression, function, and enigma.
    Stem Cells. 25 (6): 1339-47.
  2. Ortega, G. et al. (1986) Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity.
    J Immunol. 137 (10): 3240-6.
  3. Malek, T.R. et al. (1986) Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies.
    J Exp Med. 164 (3): 709-22.
  4. Codias, E.K. et al. (1990) Down-regulation of IL-2 production by activation of T cells through Ly-6A/E.
    J Immunol. 145 (5): 1407-14.
  5. Snapper, C.M. et al. (1991) Cross-linkage of Ly-6A/E induces Ca2+ translocation in the absence of phosphatidylinositol turnover and mediates proliferation of normal murine B lymphocytes.
    J Immunol. 147 (4): 1171-9.

Synonyms
Sca-1
RRID
AB_1102736
UniProt
P05533
Entrez Gene
Ly6a
GO Terms
GO:0005622 intracellular
GO:0009897 external side of plasma membrane
GO:0031225 anchored to membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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