IL-2 antibody | JES6-1A12
Rat anti mouse IL-2, clone JES6-1A12 recognizes mouse Interleukin-2. IL-2 is a cytokine required for T-cell proliferation.
Rat anti mouse IL-2, clone JES6-1A12 is reported to have neutralizing activity for IL-2 (Abrams et al. 1992) and Bihl et al. 2010).
Rat anti mouse IL-2, clone JES6-1A12 is reported to have neutralizing activity for IL-2 (Abrams et al. 1992) and Bihl et al. 2010).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- E. coli expressed recombinant mouse IL-2.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P04351 Related reagents
- Entrez Gene
- Il2 Related reagents
- GO Terms
- GO:0005125 cytokine activity
- GO:0005134 interleukin-2 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0046013 regulation of T cell homeostatic proliferation
- GO:0048304 positive regulation of isotype switching to IgG isotypes
- GO:0050672 negative regulation of lymphocyte proliferation
- GO:0050728 negative regulation of inflammatory response
- GO:0051024 positive regulation of immunoglobulin secretion
- Regulatory
- For research purposes only
Applications of IL-2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1ug/ml | 4ug/ml | |
| Immunoprecipitation | |||
| Western Blotting | 1ug/ml | 5ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- Clone JES6-1A12 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with MCA1303G clone JES6-5H4 as the detection reagent. Conjugation of MCA1303G will be required. Bio-Rad reccomends the use of LYNX rapid conjugation kits.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | 1 ml | C E P WB | ||
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | 0.5 mg | C E IF P WB | ||
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | 0.1 mg | F IF WB | ||
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | 1 mg | F | ||
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | 1 mg | C E P RE | ||
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | 0.5 ml | F | ||
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | 0.1 mg | F IF WB | ||
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | 0.5 mg | C E P | ||
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | 0.5 ml | F |
Product Specific References
References for IL-2 antibody
-
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24. -
Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
J Immunol Methods. 166 (2): 201-14. -
Rigby, S.M. et al. (2003) Total lymphoid irradiation nonmyeloablative preconditioning enriches for IL-4-producing CD4+-TNK cells and skews differentiation of immunocompetent donor CD4+ cells.
Blood. 101 (5): 2024-32. -
Eyles, J.L. et al. (2002) Negative regulation of interleukin-12 signaling by suppressor of cytokine signaling-1.
J Biol Chem. 277 (46): 43735-40. -
Kitching, A.R. et al. (2000) IL-18 has IL-12-independent effects in delayed-type hypersensitivity: studies in cell-mediated crescentic glomerulonephritis.
J Immunol. 165 (8): 4649-57. -
Bihl, F. et al. (2010) Primed antigen-specific CD4+ T cells are required for NK cell activation in vivo upon Leishmania major infection.
J Immunol. 185: 2174-81 -
Tachibana, M. et al. (2000) Coreceptor function of mutant human CD4 molecules without affinity to gp120 of human immunodeficiency virus.
J Biol Chem. 275: 20288-94.
