IL-2 antibody | JES6-1A12

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Rat anti Mouse Interleukin-2

Product Type
Monoclonal Antibody
Clone
JES6-1A12
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1493 E IP WB 0.5 mg
Rat anti mouse IL-2, clone JES6-1A12 recognizes mouse Interleukin-2. IL-2 is a cytokine required for T-cell proliferation.

Rat anti mouse IL-2, clone JES6-1A12 is reported to have neutralizing activity for IL-2 (Abrams et al. 1992) and Bihl et al. 2010).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli expressed recombinant mouse IL-2.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P04351 Related reagents
Entrez Gene
Il2 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005134 interleukin-2 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0042104 positive regulation of activated T cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046013 regulation of T cell homeostatic proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050672 negative regulation of lymphocyte proliferation
GO:0050728 negative regulation of inflammatory response
GO:0051024 positive regulation of immunoglobulin secretion
Regulatory
For research purposes only

Applications of IL-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1ug/ml 4ug/ml
Immunoprecipitation
Western Blotting 1ug/ml 5ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
Clone JES6-1A12 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with MCA1303G clone JES6-5H4 as the detection reagent. Conjugation of MCA1303G will be required. Bio-Rad reccomends the use of LYNX rapid conjugation kits.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

References for IL-2 antibody

  1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol. Rev. 127: 5-24.
  2. Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
    J. Immunol. Methods 166: 201-214.
  3. Rigby S. et al. (2003) Total lymphoid irradiation nonmyeloablative preconditioning enriches for IL-4 producing CD4+ - TNK cells and skews differentiation of immunocompetent donor CD4+ cells.
    Blood 101:2024-2032.
  4. Eyles J. et al. (2002) Negative regulation of interleukin-12 signaling by suppressor of cytokine signaling-1.
    J. Biol Chem 277:43735-43740.
  5. Kitching A. et al. (2000) IL-18 has IL-12-independent effects in delayed-type hypersensitivity : studies in cell-mediated crescentic glomerulonephritis.
    J. Immunol. 165:4649-4657.
  6. Bihl, F. et al. (2010) Primed antigen-specific CD4+ T cells are required for NK cell activation in vivo upon Leishmania major infection.
    J Immunol. 185: 2174-81
  7. Tachibana, M. et al. (2000) Coreceptor function of mutant human CD4 molecules without affinity to gp120 of human immunodeficiency virus.
    J Biol Chem. 275: 20288-94.