IL-2 antibody | JES6-1A12

Rat anti Mouse Interleukin-2

Product Type
Monoclonal Antibody
Clone
JES6-1A12
Isotype
IgG2a
Specificity
IL-2

Product Code Applications Pack Size List Price Your Price Qty
MCA1493
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IP WB 0.5 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti mouse IL-2, clone JES6-1A12 recognizes mouse Interleukin-2. IL-2 is a cytokine required for T-cell proliferation.

Rat anti mouse IL-2, clone JES6-1A12 is reported to have neutralizing activity for IL-2 (Abrams et al. 1992) and Bihl et al. 2010).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli expressed recombinant mouse IL-2.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1ug/ml 4ug/ml
Immunoprecipitation
Western Blotting 1ug/ml 5ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
Clone JES6-1A12 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with MCA1303G clone JES6-5H4 as the detection reagent. Conjugation of MCA1303G will be required. Bio-Rad reccomends the use of LYNX rapid conjugation kits.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:FITC
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
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Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)

References for IL-2 antibody

  1. Rigby, S.M. et al. (2003) Total lymphoid irradiation nonmyeloablative preconditioning enriches for IL-4-producing CD4+-TNK cells and skews differentiation of immunocompetent donor CD4+ cells.
    Blood. 101 (5): 2024-32.
  2. Eyles, J.L. et al. (2002) Negative regulation of interleukin-12 signaling by suppressor of cytokine signaling-1.
    J Biol Chem. 277 (46): 43735-40.
  3. Kitching, A.R. et al. (2000) IL-18 has IL-12-independent effects in delayed-type hypersensitivity: studies in cell-mediated crescentic glomerulonephritis.
    J Immunol. 165 (8): 4649-57.
  4. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  5. Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
    J Immunol Methods. 166 (2): 201-14.
  6. Bihl, F. et al. (2010) Primed antigen-specific CD4+ T cells are required for NK cell activation in vivo upon Leishmania major infection.
    J Immunol. 185: 2174-81
  7. Tachibana, M. et al. (2000) Coreceptor function of mutant human CD4 molecules without affinity to gp120 of human immunodeficiency virus.
    J Biol Chem. 275: 20288-94.

RRID
AB_322133
UniProt
P04351
Entrez Gene
Il2
GO Terms
GO:0005125 cytokine activity
GO:0005134 interleukin-2 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0042104 positive regulation of activated T cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046013 regulation of T cell homeostatic proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050672 negative regulation of lymphocyte proliferation
GO:0050728 negative regulation of inflammatory response
GO:0051024 positive regulation of immunoglobulin secretion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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