IgG2a antibody | AbD06344

Human anti Mouse IgG2a
- Product Type
- Monoclonal Antibody
- Clone
- AbD06344
- Isotype
- HuCAL Fab bivalent
- Specificity
- IgG2a
Human anti Mouse IgG2a, clone AbD06344 is specific for mouse IgG2a. No cross reactivity is seen with other mouse immunoglobulin sub-classes, rat immunoglobulins, or sheep, bovine and goat IgG. |
- Target Species
- Mouse
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified mouse monoclonal IgG2a.
- Approx. Protein Concentrations
- 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 6 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
1/5000 | 1/12500 |
Flow Cytometry | ![]() |
1/50 |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
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- RRID
- AB_906057
- UniProt
- P01865
- P01863
- P01864
- Entrez Gene
- Igh-1a
- Igh-1a
- Igh-1a
- GO Terms
- GO:0003823 antigen binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
HCA037



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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