F4/80 antibody | Cl:A3-1
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used for the identification of macrophages in injured soleus muscle by immunohistochemistry of formalin fixed, paraffin embedded tissue sections.
Image caption:
PCNA and F4/80 immunohistochemical expression after muscular injury. WT: wild type, KO: knockout. (A) Immunohistochemistry of PCNA. (Scale bar = 50μm), (B) Quantitative analysis of PCNA-positive cells, (C) Immunohistochemistry of F4/80. (Scale bar = 50μm), (D) Quantitative analysis of F4/80-positive cells. (B and D) Both PCNA and F4/80 expression in p21KO mice at 3 days after injury were the highest (p < 0.05).
From: Chinzei N, Hayashi S, Ueha T, Fujishiro T, Kanzaki N, Hashimoto S, et al. (2015)
P21 Deficiency Delays Regeneration of Skeletal Muscular Tissue.
PLoS ONE 10(5): e0125765.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the detection of infiltrating macrophages in tumor tissue by immunofluorescence.
Image caption:
Presence of inflammatory cells in tumor tissue. Macrophage and neutrophil infiltration are unaffected by loss of serglycin in RIP1-Tag2 tumors. Tumor sections from 15w RTposSGwt and RTposSGko mice were immunostained from the neutrophil and macrophage markers Gr-1 (a) and F4/80 (b) respectively. For Gr-1, the number of positive cells/mm2 was calculated (c) and there was no difference in infiltration of neutrophils between the two groups. For F4/80, the area of positive staining was measured (d) and although there was a slight trend to decreased macrophage infiltration in serglycin deficient animals, this was not significant. Each data point in represents an individual animal. Statistical analysis was performed using a two-tailed Mann-Whitney test. Error bars represent mean ± SEM.
From: Hamilton A, Basic V, Andersson S, Abrink M, Ringvall M (2015)
Loss of Serglycin Promotes Primary Tumor Growth and Vessel Functionality in the RIP1-Tag2 Mouse Model for Spontaneous Insulinoma Formation.
PLoS ONE 10(5): e0126688.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 (MCA497GA) antibody used for the detection of macrophage processes in mouse kidney tissue sections by immunofluorescence.
Image caption:
Dual immunofluorescence staining showing the association of F4/80+ cells with deposited C3. Shown is a representative immunofluorescence micrograph of a Crry−/−C3−/− kidney 7 days after transplantation into a wildtype recipient. Considerable deposition of C3 (green) is evident in the basolateral aspects of tubules, along with closely approximated F4/80+ cellular processes (red).
From: Chaves LD, Bao L, Wang Y, Chang A, Haas M, Quigg RJ (2014)
Loss of CD11b Exacerbates Murine Complement-Mediated Tubulointerstitial Nephritis.
PLoS ONE 9(3): e92051.Published customer image:
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the detection of infiltrating macrophages in tumor tissue by immunofluorescence.
Image caption:
Presence of inflammatory cells in tumor tissue. Macrophage and neutrophil infiltration are unaffected by loss of serglycin in RIP1-Tag2 tumors. Tumor sections from 15w RTposSGwt and RTposSGko mice were immunostained from the neutrophil and macrophage markers Gr-1 (a) and F4/80 (b) respectively. For Gr-1, the number of positive cells/mm2 was calculated (c) and there was no difference in infiltration of neutrophils between the two groups. For F4/80, the area of positive staining was measured (d) and although there was a slight trend to decreased macrophage infiltration in serglycin deficient animals, this was not significant. Each data point in represents an individual animal. Statistical analysis was performed using a two-tailed Mann-Whitney test. Error bars represent mean ± SEM.
From: Hamilton A, Basic V, Andersson S, Abrink M, Ringvall M (2015)
Loss of Serglycin Promotes Primary Tumor Growth and Vessel Functionality in the RIP1-Tag2 Mouse Model for Spontaneous Insulinoma Formation.
PLoS ONE 10(5): e0126688.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC-conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497F) used for the detection of F4/80 expressing cells in peritoneal cell exudates by flow cytometry.
Image caption:
Identification of resident peritoneal Mø subsets. PC from C57BL/6 were harvested and stained with fluorochrome-labeled antibodies directed against F4/80, CD19, CD11c and IAb for flow cytometry analysis. (A) Doublet cells were excluded according to forward scatter profiles (FSC-A and FSC-H). Subsequently, (B) CD19 high cells and (C) CD11c high cells were also excluded, and (D) F4/80+ cells were selected. (E) F4/80 and IAb expression defined three populations: LPM (F4/80highIAb-neg), SPM (F4/80lowIAb-high) and granulocytes (F4/80lowIAb-neg). These three subpopulations were purified by cell sorting on a FACS Vantage, and their morphology was evaluated ex vivo from cytospin slides (F), or after in vitro culture in chamber slides for 12 h (G). Slides were stained with hematoxylin and eosin (H&E) and analyzed by optical microscopy (40×).
From: Cassado AdA, de Albuquerque JAT, Sardinha LR, Buzzo CdL, Faustino L, Nascimento R, et al. (2011)
Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli.
PLoS ONE 6(7): e22141.
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FITC-conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497F) used for the detection of F4/80 expressing cells in peritoneal cell exudates by flow cytometry.
Image caption:
Zymosan and T. cruzi injection alters the Mø compartment of PerC. C57BL/6 mice were injected i.p. with zymosan (1 mg/mouse) or T. cruzi (106 parasites/mouse) and at 30 min (A) or 48 h (B) after stimulation, PC from naive and injected mice were harvested and stained as described in M&M. Sequential gates were made as shown in Fig. S1. Plots show the frequencies of each subpopulation. (C) F4/80lowMHCIIint cells present within PerC 48 h after injections were evaluated according the expression of Ly6C. Gray lines represent FMO [23], [25], the black lines show F4/80lowMHCIIint cells from zymosan- (hairline) or T. cruzi-(bold line)-exposed PerC. (D) Total numbers of SPM, LPM and monocytes 48 h after zymosan or T. cruzi exposure (within Mø gate) are shown in panel. Data are representative of more than 3 independent experiments.
From: Cassado AdA, de Albuquerque JAT, Sardinha LR, Buzzo CdL, Faustino L, Nascimento R, et al. (2011)
Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli.
PLoS ONE 6(7): e22141.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC-conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497F) used for the detection of F4/80 expressing cells in peritoneal cell exudates by flow cytometry.
Image caption:
SPM are more responsive than LPM and monocytes to infectious stimuli. C57BL/6 mice were injected i.p. with zymosan (1 mg/mouse) or T. cruzi (106 parasites/mouse) and PCs were harvested 30 min or 48 h after stimulation. (A) SPM and LPM from C57BL/6 zymosan-exposed mice were FACS-sorted 30 min after injection and the presence of internalized zymosan particles was observed by optical microscopy. Slides were made with 105 cells from purified Mø subsets, stained with H&E and analyzed by optical microscopy (40×). (B) Numbers represent the mean ±SD of internalized zymosan particles per cell in each Mø subset. *** p<0.001 when compared to the LPM group. (C) PC from control or 48 h-exposed mice were cultured for 6 h in the presence of brefeldin A with or without LPS (1 μg/ml) plus rIFN-γ (5 ng/ml). Titles above plots indicate in vivo - in vitro stimulations. Values inside gates represent the frequencies of IL-12-producing cells in each subpopulation. Data are representative of more than 3 independent experiments.
From: Cassado AdA, de Albuquerque JAT, Sardinha LR, Buzzo CdL, Faustino L, Nascimento R, et al. (2011)
Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli.
PLoS ONE 6(7): e22141.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used for the detection of macrophages in mouse lung and pancreas by immunohistochemistery on formalin fixed, paraffin tissue sections.
Image caption:
Changes in pancreatic and lung F4/80+ macrophage number during acute pancreatitis. Representative photomicrographs of pancreas (A) and lung (C) sections stained for F4/80 as indicator of macrophage infiltration (brown) 24 h after pancreatitis induction (original magnification, ×20). Quantification of F4/80+ cells showed a significantly increased enrichment in the pancreas from 9 h following pancreatitis compared to sham controls (B). No significant difference was observed in the number of lung F4/80+ cells between pancreatitis and sham control groups (D). Bars show mean ± SEM, n = 10 per group. **P<0.01, ***P<0.001, by two-tailed Student t-test.
From: Akbarshahi H, Menzel M, Posaric Bauden M, Rosendahl A, Andersson R (2012)
Enrichment of Murine CD68+CCR2+ and CD68+CD206+ Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.
PLoS ONE 7(10): e42654.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used for the evaluation of F4/80 expression on isolated Kuppfer and peritoneal cells by flow cytometry.
Image caption:
CLEC4F is co-expressed with F4/80 on liver Kupffer cells.
(A) CLEC4F and F4/80 immunohistochemistry of parafilm-embedded liver sections from wild-type and Clec4f−/− mice. (B) Double immunofluorescence of CLEC4F and F4/80 in wild-type livers was performed. Nuclei were counterstained with Hoechst 33342. Signals were determined by confocal microscope (magnification 10×63). (C) Coexpression of CLEC4F and F4/80 on Kupffer cells, but not peritoneal macrophages. Cells were double stained with Alexa Fluor 647-conjugated anti-F4/80 and PE-conjugated anti-CLEC4F mAb. Alexa Fluor 647-conjugated rat IgG2b and PE-conjugated mIgG1 were used as isotype controls.
From: Yang C-Y, Chen J-B, Tsai T-F, Tsai Y-C, Tsai C-Y, Liang P-H, et al. (2013)
CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver.
PLoS ONE 8(6): e65070.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the evaluation of F4/80 expression on thymic myeloid cells by flow cytometry.
Image caption:
Heterogeneous phenotype of thymic resident myeloid cells. (A) Thymic stromal cells were enriched by collagenase/dispase digestion followed by percoll density gradient centrifugation. Cells were then stained with anti-CD11b and anti-F4/80 and analyzed by FACS. Red circles mark the three different cell populations discussed in the text. In this experiment, populations #1, #2 and #3 represented 0.02%, 0.43% and 0.61% of the analyzed cells, respectively. While the absolute cell number varied as a result of the preparation, the percentage of each cell type relative to each other was consistent over many experiments. (B) Size and surface marker expression (blue lines) of each population was determined by FACS. Populations were identified as in (A) and stained with the antibodies as indicated. Isotype controls (red lines) were used to correct differences in the autofluorescence of each cell population. (C) Individual cells from populations #2 and #3 were sorted onto glass slides followed by staining with H&E. Data are representative of more than ten experiments (a and b) and three experiments (c).
From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010)
Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used for the evaluation of F4/80 expression on thymic myeloid cells following irradiation by immunofluorescence.
Image caption:
Time course analysis of F4/80 positive cells following irradiation.
Thymuses from wild type B6 mice were harvested and frozen post-irradiation at the indicated times (1 hour through 24 hours). Sections were stained with anti-F4/80 (red), which identifies macrophages and eosinophils. Panel labeled "control" was a section stained with secondary antibody only. Panel labeled “w/o irr.” was a section from a nonirradiated mouse. Original magnification was 200X.
From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010)
Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the evaluation of F4/80 expression on thymic myeloid cells following irradiation by flow cytometry.
Image caption:
Influx of innate cells rapidly declines twenty-four hours after irradiation. Thymuses were harvested from mice at the indicated times after irradiation. Stromal cells were enriched as described and stained with anti-CD11b, -F4/80, SiglecF and -Ly-6G. Numbers in the plots are the percentage of cells within the electronic gate. N = 6 and the data show the results from two independent experiments. The mean is shown by a horizontal bar and statistics were calculated with 2-tailed, nonpaired Mann-Whitney test. * = <0.01; ** = <0.001; *** = <0.0001..
From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010)
Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the evaluation of F4/80 expression on spleen and bone marrow cells by flow cytometry.
Image caption:
FACS analyses of the innate cells in the spleen and bone marrow from WT and Csf op/op mice. Cells from the spleen and bone marrow from WT and Csfop/op mice were stained for CD11b and F4/80. Eosinophils and neutrophils were identified by staining cells for CD11b and SiglecF or CD11b and Gr-1, respectively. Percent of cells in each area is indicated in the Figure.
From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010)
Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the identification of macrophages in mouse spleen using immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Depletion of F4/80-expressing cells in spleens derived from clodrolip-treated Ptchflox/floxERT2+/− mice. Immunohistochemical analysis using an anti-F4/80 antibody of paraffin-embedded spleens of Ptchflox/floxERT2+/− mice treated with empty liposomes or clodrolip.
From: König S, Nitzki F, Uhmann A, Dittmann K, Theiss-Suennemann J, Herrmann M, et al. (2014)
Depletion of Cutaneous Macrophages and Dendritic Cells Promotes Growth of Basal Cell Carcinoma in Mice.
PLoS ONE 9(4): e93555.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used for the identification of infiltrating macrophages in tumor tissue by immunofluorescence.
Image caption:
CSF1 has no effect on tumor growth but increases percent tumor TEMs and augments angiogenesis. (A) After two weeks of treatment, tumors were removed, homogenized and immunostained with antibodies specific for F4/80 and Tie2 to identify total F4/80+ cells and F4/80+/Tie2+ cells (Tie2-expressing macrophages, TEMs). While there was a marked increase in total F4/80+ macrophages with CSF1 treatment, the percent of F4/80+/Tie2+ TEMs was significantly increased in response to CSF1 suggesting a regulatory role for CSF1 in expanding the TEM population. N = 5 mice per group and results represent the mean ± SEM of total F4/80+ and F4/80+/Tie2+ TEMs within the tumors. (B, top and bottom left) PyMT tumors without CSF1 treatment and (top and bottom right) with CSF1 treatment immunostained with CD31 for blood vessels, F4/80 for macrophages, Tie2 for F4/80+/Tie2+ TEMS, and DAPI. Confocal images (using 60× objective (top) and with 3× zoom (bottom) suggest an increase in both F4/80 macrophages and F4/80+/Tie2+ TEMS in the CSF1-treated tumors. Multiply overlap indicates those areas where F4/80 and Tie2 positivity overlap. Individual stains are in Supplementary Figure 3. (C, top) Orthotopically implanted PyMT mammary tumors in wild type C57Bl/6 female mice were allowed to become palpable then intraperitoneally treated with PBS (PBS), CSF1 (100 ng in 100 μls) (CSF1), a neutralizing antibody for the CSF1R (50 mg/kg) 4 hours prior to CSF1 treatment (100 ng in 100 μls) (CSF1R NAb+CSF1), the CSF1R antibody alone (CSF1R NAb), an isotype antibody (50 mg/kg) 4 hours prior to CSF1 (100 ng in 100 μls) treatment (CSF1+IgG), or the isotype antibody alone (IgG) three times per week for two additional weeks. The tumors were immunostained with a CD31-Alexa Flour 546 antibody to recognize endothelial cells that comprise blood vessels. Qualitatively, CSF1 treatment increased the percent of CD31-postitive pixels per high powered field compared to PBS treated tumors, while the neutralizing antibody to CSF1R suppressed the CSF1 effect on angiogenesis. (B, bottom) Quantitatively, the percent of CD31+ pixels per high powered field were quantified as blood vessels (angiogenesis) using Adobe Photoshop histogram analysis. CSF1 treatment significantly increased CD31-positive pixels (angiogenesis) compared to PBS. The neutralizing antibody for CSF1R significantly reduced the ability of CSF1 to up-regulate angiogenesis. N = 5 mice per group and results represent the mean ± SEM of percent CD31-positive pixels per high powered field (HPF).
From: Forget MA, Voorhees JL, Cole SL, Dakhlallah D, Patterson IL, Gross AC, et al. (2014)
Macrophage Colony-Stimulating Factor Augments Tie2-Expressing Monocyte Differentiation, Angiogenic Function, and Recruitment in a Mouse Model of Breast Cancer.
PLoS ONE 9(6): e98623.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used for the identification of infiltrating macrophages in the spleen of Entamoeba histolytica infected mice by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Histological and immunohistochemical characterization of cell infiltrates during ALA. (A) H&E staining of mouse liver abscesses (indicated by the square in the top row of images) at the indicated times post-infection with E. histolytica trophozoites. (B) PAS staining shows E. histolytica trophozoites (arrowheads) within the abscess. (C and D) Tissue sections were stained with anti-7/4 (C) and anti-F4/80 (D) antibodies followed by HRP-conjugated secondary antibody to detect neutrophils and macrophages, respectively (brown).
From: Helk E, Bernin H, Ernst T, Ittrich H, Jacobs T, Heeren J, et al. (2013)
TNFα-Mediated Liver Destruction by Kupffer Cells and Ly6Chi Monocytes during Entamoeba histolytica Infection.
PLoS Pathog 9(1): e1003096.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for immunohistochemistry on mouse brain sections
Image caption:
Representative photographs of cortical area in (a) CD40 def. and (b) wild-type mice stained with F4/80 antibody at 8 to 10 weeks old (each bar represents 0.1 mm).
From: Laporte et al.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used for the detection of microglia in mouse brain by immunofluorescence
Image caption:
Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real–time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean ± SEM; *P<0.05, **P<0.01, and ***P<0.001 versus sham group, #P<0.05, ##P<0.01, and ###P<0.001 versus vehicle group (n = 6–9 mice/group for BDNF protein and 7–9 mice/group for phospho-CREB, one-way ANOVA; n = 6–8 mice/group for BDNF mRNA, one-way ANOVA or Student's t-test). The scale bar is 50 μm. Ipsi: ipsilateral cortex; Contra: contralateral cortex.
From: Wu C-H, Hung T-H, Chen C-C, Ke C-H, Lee C-Y, et al. (2014)
Post-Injury Treatment with 7,8-Dihydroxyflavone, a TrkB Receptor Agonist, Protects against Experimental Traumatic Brain Injury via PI3K/Akt Signaling.
PLoS ONE 9(11): e113397.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the detection of infiltrating macrophages in mouse kidney by immunohistochemistry on paraffin embedded material
Image caption:
Reduction in cellular infiltration and inflammatory markers in UUO kidneys exposed to telmisartan or PXS64. Untreated UUO kidneys showed increased F4/80, CD68 and CD45 positively stained cells as compared to the sham operated control animals. PXS64 significantly reduced F4/80 and CD45 positive stained cells (Fig. 6C and E) with a trend to a reduction in CD68 cells, although this was not statistically significant (Fig. 6D). Telmisartan treated kidneys showed a reduction in F4/80 positive cells but no difference in CD45 or CD68 stained cells, suggesting a differential action of PXS64 and telmisartan in modifying cellular infiltration. Results are presented as mean showed (n = 8, *P<0.05 vs. UUO, ** P<0.01 vs. UUO). Magnification x 400.
From: Zhang J, Wong MG, Wong M, Gross S, Chen J, et al. (2015)
A Cationic-Independent Mannose 6-Phosphate Receptor Inhibitor (PXS64) Ameliorates Kidney Fibrosis by Inhibiting Activation of Transforming Growth Factor-β1.
PLoS ONE 10(2): e0116888.
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Rat anti Mouse F4/80 antibody, clone Cl:A3-1 (MCA497G) used for the identification of macrophages in a murine colitis model by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Nur77-/- mice show more macrophage and T-cell influx in TNBS-induced colitis. The numbers of T-cells in the mucosa were counted and the influx was calculated by taking the ratio of Healthy / TNBS. Representative photomicrographs of sections stained for T-cells (anti-CD3) are shown (A). mRNA was extracted from colon and qPCR was performed for FoxP3 (B). cDNA levels were normalized for 36B4 housekeeping gene expression. Macrophage surface was quantified in the mucosa and the number of macrophages was counted in the muscle. The ratio of Healthy / TNBS was calculated. Representative photomicrographs of sections stained for macrophages (anti-F4/80) are shown (C). The numbers of Ki67 positive cells were counted per crypt and representative photomicrographs are shown (D). Colon lysates were prepared to determine IL-6, TNFα, and MCP-1 levels in the colon (E). A and C Original magnification x100, D x200. Each symbol represents 1 animal. Data are presented as mean ±SEM; *p<0.05, **p<0.01, ***p<0.001. Scale bars represent 1mm. nr = number, AU = Arbitrary units, FoxP3 = Forkhead box P3 (marker for regulatory T-cells).
From: Hamers AAJ, van Dam L, Teixeira Duarte JM, Vos M, Marinković G, van Tiel CM, et al. (2015)
Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages.
PLoS ONE 10(8): e0133598.
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Rat anti MouseF4/80 antibody, clone A3-1 (MCA497R) used for the identification of macrophages in murine kidney by immunofluorescence and immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Clodronate liposome mediated macrophage depletion in Col4a3KO mice. (A) KO male mice were dosed intraperitonally with CL (KO+CL) or PBSL (KO+PBSL) as control. Animals were entered in the study at the age of 4 weeks and were continually dosed until the age of 8 weeks. Injections were repeated every second day, except for the first two doses, which were injected on consecutive days. (B-D) CL significantly reduced F4/80 positive macrophage infiltrates in KO+CL kidney as compared to KO+PBS or KO mice at the age of 8 weeks. (B, C) Representative images and quantitative assessment of F4/80 stained macrophages in kidney sections from KO+CL and KO+PBSL reveal marked reduction in macrophage infiltrates following CL dosing. Scale bar: 100 μm. (D) Real-time PCR analysis of F4/80 mRNA expression in KO+CL, KO+PBSL, KO, and WT littermates at the age of 8 weeks. Significant reduction in F4/80 mRNA expression following CL dosing was observed. PBSL did not affect endogenous F4/80 mRNA expression as shown by similar F4/80 mRNA expression in KO and KO+PBSL mice. (E) Real-time PCR analysis of CD204 and CD206 mRNA showed significant reduction in the expression of both genes in KO kidney upon CL treatment. (F) Representative images and quantitative assessment of F4/80 and CD206 stained kidney sections from KO+CL and KO+PBSL revealed marked reduction in M1 (F4/80+CD206-) and M2 (F4/80+CD206+) macrophages. Asterisk: F4/80+CD206- M1 macrophages, arrow: F4/80+CD206+ M2 macrophages. Scale bar: 20 μm. Welch corrected ANOVA followed by posthoc pairwise t-test with a Bonferroni correction (C,D,E) and unpaired t-test (F) was used to evaluate differences between groups. (C,D,E,F) n = 5 mice per group. CL: clodronate liposomes; PBSL: PBS liposomes.
From: Kim M, Piaia A, Shenoy N, Kagan D, Gapp B, Kueng B, et al. (2015)
Progression of Alport Kidney Disease in Col4a3 Knock Out Mice Is Independent of Sex or Macrophage Depletion by Clodronate Treatment.
PLoS ONE 10(11): e0141231.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the identification of murine macrophages in the kidney by immunohistochemistry on formalin fixed cryosections.
Image caption:
Immunostainings of macrophages and neutrophils in the kidneys of M-Rac1 FC and KO mice. (A) Representative micrographs of F4/80 immunostaining in the kidneys of Vehicle- or LPS-injected M-Rac1 FC and KO mice. Original magnification x 200. (B) Quantitative analysis of F4/80-positive macrophages. Data are means ± s.e.m. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 5 per each group. (C) Representative micrographs of Ly-6B.2 (mAb 7/4) immunostaining. (D) Quantitative analysis of Ly-6B.2-positive neutrophils. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 6 per group.
From: Citation: Nagase M, Kurihara H, Aiba A, Young MJ, Sakai T (2016)
Deletion of Rac1GTPase in the Myeloid Lineage Protects against Inflammation-Mediated Kidney Injury in Mice.
PLoS ONE 11(3): e0150886.
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Alexa Fluor® 647 conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497A647) used for the detection of murine Kupffer cells by immunofluorescence
Image caption:
Hepatic stellate cells are the major source of CXCL1, as shown by both quantification of secretion and in situ localization. (A) Quantification of CXCL1 secretion in enriched fractions of hepatocytes, KCs, LSECs and HSCs, freshly isolated and stimulated in vitro with LPS (1 ng/mL LPS, black squares) during 24 hours. Data are representative of three separate experiments with six mice in each group; #P<.05. (B) In-situ localization of CXCL1 in the liver. Immunofluorescent detection for CXCL1 (red) and liver cells nuclei (blue) for nuclei first shows CXCL1 expression in the sinusoids throughout liver parenchyma. (C) Higher resolution shows that CXCL1 (red) is expressed by sub-endothelial cells, which also store retinol droplets in separate compartments, as shown by CRBP1 staining (green). The Cellular Retinol Binding Protein-1 (CRBP-1) is the best marker to detect simultaneously both resting (Glial Fibrillary Acidic Protein, GFAP+) and activated (α-Smooth Muscle Actin, αSMA+) stellate cells in situ. Alexa Fluor-546-CXCL1 (red) staining does not colocalize either with Tie2-GFP in LSECs (green, upper panel), or F4/80 in KCs (blue, middle panel), but with AlexaFluor-488-CRBP1 (green, lower panel), staining both resting and activated HSCs. TOPRO3 was used for nuclei vizualisation.
From: Bigorgne AE, John B, Ebrahimkhani MR, Shimizu-Albergine M, Campbell JS, Crispe IN (2016)
TLR4-Dependent Secretion by Hepatic Stellate Cells of the Neutrophil-Chemoattractant CXCL1 Mediates Liver Response to Gut Microbiota.
PLoS ONE 11(3): e0151063.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used for the demonstration of microglia in mouse brain by immunohistochemistry on formalin fixed 40μ vibratome sections.
Image caption:
CN-105 reduces brain microglial activation after ICH.
(A) Representative images of F4/80-labelled microglia in ipsilateral hippocampi at 5 days after ICH in mice treated with vehicle or CN-105.
From: Lei B, James ML, Liu J, Zhou G, Venkatraman TN, Lascola CD, Acheson SK, Dubois LG, Laskowitz DT, Wang H.
Neuroprotective pentapeptide CN-105 improves functional and histological outcomes in a murine model of intracerebral hemorrhage.
Sci Rep. 2016 Oct 7;6:34834.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used for the identifcation of macrophages in kidney sections by immunohistochemistry on cryosections.
Image caption:
Renal markers of inflammation at Week 32.
F4/80 representative images at 200x magnification, E. TGF-β mRNA expression, F. MCP-1 mRNA expression. Results are expressed as mean ± SEM, N = 4–6. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 compared to CC.
From: Glastras SJ, Chen H, Tsang M, Teh R, McGrath RT, Zaky A, et al. (2017)
The renal consequences of maternal obesity in offspring are overwhelmed by postnatal high fat diet.
PLoS ONE 12(2): e0172644.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used for the identification of macrophages in kidneys and livers of mice by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Focal expression of Irga6 in healthy mouse liver and kidney.
T cells and macrophages/dendritic cells are present in the mononuclear cores of the liver and kidney patches. Wax sections of the livers and kidneys from adult C57BL/6 mice were stained for Irga6 (brown/red) and CD3 or F4/80 (blue). For Irga6 and CD3 double staining in liver, consecutive serial sections were analysed (numbered 1, 2, 3, 4). Arrows point to CD3 and F4/80 positive cells. The microscope magnification is 200×; frames show enlargements.
From: Zeng J, Howard JC.
Spontaneous focal activation of invariant natural killer T (iNKT) cells in mouse liver and kidney.
BMC Biol. 2010 Nov 30;8:142.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F480, clone A3-1 (MCA497R) used for the demonstration of Kupffer cells in mouse liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Expression of F4/80 and CD68 in liver tissue assessed by immunostaining using anti-F4/80 or anti-CD68 antibody.
From: Citation: Ibusuki R, Uto H, Oda K, Ohshige A, Tabu K, Mawatari S, et al. (2017)
Human neutrophil peptide-1 promotes alcohol-induced hepatic fibrosis and hepatocyte apoptosis.
PLoS ONE 12(4): e0174913.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used to identify macrophages in the murine vastus lateralis by immunofluorescence on cryostat sections.
Image caption:
Combination of bone fracture and alendronate treatment causes loss of myogenic cells.
PBS (Control group) or alendronate (Alendronate group) was subcutaneously injected three times at seven-day intervals. The femur was broken and vastus lateralis muscle injured by cold shock on day 14. Then the vastus lateralis muscle was removed on day 1, 4, or 7 after the bone fracture and cold injury. Sections were subjected to immunostaining with antibodies to M-cadherin (green in A-F), F4/80 (green in G-L), CD31 (green in M-R), and laminin α2 (red in A-R). Nuclei were stained with DAPI (blue in A-R). Arrows represent M-cadherin-positive muscle satellite cells in (C). Scale bars, 40 μm in (A-F), 20 μm in (G-R). Images represent a part of each original picture. Numbers represent numbers of M-cadherin-positive muscle satellite cells/myogenic progenitor cells in each original picture (0.17 mm2) in (A-F). Percentages of F4/80-positive cell-occupied area of each original picture (0.68 mm2) are shown in (G-L). Numbers of CD31-positive capillaries per mm2 of each original picture are shown in (O and R).
From: Kawada S, Harada A, Hashimoto N (2017)
Impairment of cold injury-induced muscle regeneration in mice receiving a combination of bone fracture and alendronate treatment.
PLoS ONE 12(7): e0181457.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used to identify infiltrating macrophages in mice by immunohistochemistry on formalin fixed, paraffin embedded tissue sections
Image caption:
Middle ear histology indicates chronic otitis media in Nischedsn/edsn mice.
(J) Immunohistochemical staining of a Nischedsn/edsn ear with OM using an F4/80 antibody shows a cellular exudate rich in macrophages (brown). Representative image from four mice scale bar =100μm.
From: Crompton M, Purnell T, Tyrer HE, Parker A, Ball G, Hardisty-Hughes RE, et al. (2017)
A mutation in Nischarin causes otitis media via LIMK1 and NF-κB pathways.
PLoS Genet 13(8): e1006969.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the identification of macrophages in mouse spleen sections by immunofluorescence.
Image caption:
Localization of IgG2c+ (red) plasma cells in the spleens of 4 month old w.t. and MFG-E8-/- mice was examined by immunofluorescence staining. Red pulp macrophages and Marginal zone macrophages were visualized by anti-F4/80(green, upper) and anti-CD169 (green, lower), respectively. The images are representative of at least 5 mice in each strain.
From: Peng Y (2018)
B cell responses to apoptotic cells in MFG-E8-/- mice.
PLoS ONE 13(10): e0205172.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Moouse F4/80 antibody, clone A3-1 (MCA497G) used to identify macrophages in mouse liver by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
F4/80 staining. F4/80 activities in livers are examined at 1 and 2 days with immunostaining. (A) 1 day; (B)2 days. For all panels, representative images are shown and arrows denote positive staining. ns p ≥0.05, * p <0.05.
From: Wang H, Zhang H, Wang Y, Yang L, Wang D.
Embelin can protect mice from thioacetamide-induced acute liver injury.
Biomed Pharmacother. 2019 Oct;118:109360.
doi: 10.1016/j.biopha.2019.109360.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to evaluate F4/80 expression in murine kidney by western blotting and immunofluorescence.
Image caption:
UMOD expression amplifies UUO‐associated interstitial inflammation. Interstitial inflammation is a hallmark feature of kidney fibrosis. Interstitial macrophage density, quantified by kidney F4/80 protein western blotting (n = 5 for sham groups; n = 7 for UUO groups), was significantly lower in the UMOD−/− groups by 41–58% at all time‐points (A, B). Representative day 21 immunofluorescence photomicrographs illustrate a smaller interstitial area expressing the F4/80 macrophage protein (green) in the UMOD−/− kidney (C). Kidney MCP‐1 mRNA levels were significantly lower in the UMOD−/− mice on UUO days 7, 14, and 21 (n = 3 for sham groups; n = 7 for UUO groups) (D). Bar graphs represent means +1SEM; * indicates P value <0.05. Photomicrograph magnification: ×40. Scale bar: 25 μm.
From: Maydan O, McDade PG, Liu Y, Wu XR, Matsell DG, Eddy AA.
Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy.
Physiol Rep. 2018 Mar;6(6):e13654.
doi:10.14814/phy2.13654.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the evaluation of F4/80 expression on thymic cells by flow cytometry.
Image caption:
Impaired clearance of apoptotic thymocytes in Csf1op/op mice. (A) Thymic stromal cell subpopulations from WT (Csf1op/+) and osteopetrosis (Csf1op/op) mice were stained with the indicated antibodies and analyzed by FACS. Numbers indicate the percentage of cells within the circles. The red circle marks population #1, which is not present in Csf1op/op mice. (B) Comparison of thymic stromal cell subpopulations between WT (Csf1op/+) and osteopetrosis (Csf1op/op) mice 16 hours after irradiation. Thymic stromal cells were enriched and stained for CD11b and F4/80. (C) Representative images showing the frequency of apoptotic cells in WT (Csf1op/+) and Csf1op/op mice in nonirradiated mice in mice 24 hrs receiving 1Gy irradiation. Apoptotic cells were detected by TUNEL (green) and their cortical localization was visualized by counterstaining of thymic sections with an antibody against DEC205 (red). Original magnification was 100X. Sections are representative of multiple samples from more than ten experiments.
From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010)
Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the identification of macrophages in formalin fixed, paraffin embedded murine skin tissue sections by immunohistochemistry.
Image caption:
L-tryptophan administration reverses stress-induced alterations on inflammatory response and lipid peroxidation. Mice were daily submitted to restraint stress and treated with L-tryptophan (Trp) or vehicle until euthanasia. Two days after the beginning of the stress protocol, two full-thickness excisional lesions (8-mm diameter) were made on the dorsal skin. Five days after wounding, lesions were collected and the number of myeloperoxidase (MPO)-positive neutrophils (B), F4/80-positive macrophages (C) and CD-3 positive T lymphocytes (D) were counted. Representative images (A) of staining for neutrophils, macrophages and T cells on wound area were presented. In addition, the protein levels of monocyte chemotactic protein-1 (MCP-1) (12 kDa) (E) were estimated in wound lysate by western-blotting. Representative images for immunoblotting for MCP-1 (F) were presented. The β-actin (42 kDa) was used as a loading control protein. Vertical black lines show non-adjacent bands from the same blot. To evaluate the oxidative damage in lipids, the levels of lipid peroxides (G) were measured in wound lysate using colorimetric assay. Data (n = 5) are presented as mean ± SEM. #p<0.05 vs. stressed (ST) group. Bar = 50 μm.
From: Bandeira LG, Bortolot BS, Cecatto MJ, Monte-Alto-Costa A, Romana-Souza B (2015)
Exogenous Tryptophan Promotes Cutaneous Wound Healing of Chronically Stressed Mice through Inhibition of TNF-α and IDO Activation.
PLoS ONE 10(6): e0128439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone 3A-1 (MCA497R) used to identify macrophages in mouse muscle graft sections by immunofluorescence.
Image caption:
Autografted and allografted muscles: morphology and osteopontin expression. Cryosections of EDL muscle grafts at 5 (a, b) or 3 (c, d) days post-surgery. a H&E-stained sections. Arrows indicate regenerating fibres (small fibres with centrally located nuclei). b Sections stained with anti-MyHCemb (green), anti-laminin (red) and DAPI, as used for quantitation of regenerating fibres. c Sections stained with anti-osteopontin (red), anti-desmin (myoblasts; green) and DAPI. In wild-type (WT) EDL muscle grafted into a wild-type host, anti-osteopontin stained both myoblasts (yellow) and non-myoblast mononuclear cells (red). In osteopontin-null (KO) muscle grafted into an osteopontin-null host, no osteopontin was detected. In allografted osteopontin-null muscle in a wild-type host, osteopontin was only detected in non-myoblast mononuclear cells. In allografted wild-type muscle in an osteopontin-null host, osteopontin was only detected in myoblasts. Arrows and arrowheads indicate myoblasts and non-myoblast mononuclear cells, respectively. d Sections stained with anti-osteopontin (red), anti-F4/80 (macrophages; green) and DAPI. In autografted wild-type muscle, anti-osteopontin stained macrophages (*; yellow) and some additional structures (arrows; red). In autografted osteopontin-null muscle, no osteopontin was detected. In allografted osteopontin-null muscle in a wild-type host, osteopontin was only observed in macrophages (*; yellow). In allografted wild-type muscle in an osteopontin-null host, weak osteopontin staining was observed in some non-macrophage structures (arrows), but not in macrophages (†; green). Bars = 50μm
From: Wasgewatte Wijesinghe DK, Mackie EJ, Pagel CN.
Normal inflammation and regeneration of muscle following injury require osteopontin from both muscle and non-muscle cells. Skelet Muscle. 2019; 9(1):6.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the identification of macrophages in tumor tissues by immunohistochemistry on formalin fixed cryosections. Comparison is with the distribution of CD11c+ dendritic cells (MCA1369GA) in similar tissue sections.
Image caption:
IHC analysis of Colon-26 tumors untreated and INT230-6-treated tumors ~10 days post dose. Representative images (40× magnification) of IHC staining for F4/80 (macrophages) and CD11c (dendritic cells). In general, the staining appeared more evident in the INT230-6-treated tumors, particularly toward the more necrotic areas.
From: Bender LH, Abbate F, Walters IB.
Intratumoral Administration of a Novel Cytotoxic Formulation with Strong Tissue Dispersive Properties Regresses Tumor Growth and Elicits Systemic Adaptive Immunity in In Vivo Models.
Int J Mol Sci. 2020;2 (12):E4493.
doi:10.3390/ijms21124493
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Pacific Blue conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497PB) used for the dendritic cell gating process from mouse white adipose tissue by flow cytometry.
Image caption:
A representative gating scheme of dendritic cell and macrophage populations in the epididymal white adipose tissues. For analysis of dendritic cell and macrophage populations, stromal vascular cells obtained from epididymal white adipose tissues were stained with FITC–conjugated anti-CD206, PE–conjugated anti-CD11c, PerCP–conjugated anti-CD45, PE-Cy7–conjugated anti-F4/80*, APC–conjugated anti-CD64 and APC-Cy7–conjugated anti-MHC class II antibodies.
From: Choi EW, Lee M, Song JW, Kim K, Lee J, Yang J, Lee SH, Kim IY, Choi JH, Seong JK.
Fas mutation reduces obesity by increasing IL-4 and IL-10 expression and promoting white adipose tissue browning.
Sci Rep. 2020 Jul 20;10(1):12001.
doi: 10.1038/s41598-020-68971-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License. * should read "Pacific Blue® conjugated F4/80.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used for the identification of macrophages in murine liver sections by immunohistochemistry on formalin fixed, paraffin embedded material.
Image caption:
Protective effect of clusterin in the pathology of WD-induced NAFLD.(B) Immunostaining for F4/80 in the liver of wild type, wCLU-tg, and CLU-KO mice fed CHOW or WD. *P <0.05; **P <0.01; ***P <0.001. Magnification, × 200.
From: Park JS, Lee WK, Kim HS, Seo JA, Kim DH, Han HC, Min BH.
Clusterin overexpression protects against western diet-induced obesity and NAFLD.
Sci Rep. 2020 Oct 15;10(1):17484.
doi: 10.1038/s41598-020-73927-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to highlight macrophages in murine kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Lithium treatment induces change in M1/M2 phenotype macrophages.
A) Representative areas of macrophage infiltration by F4/80 staining in the cortical area. Arrows denote positive brown staining. Scale bars represent 100 μm. B) Quantitative analysis related to E (n = 4).
From: Teixeira DE, Peruchetti DB, Silva LS, Silva-Aguiar RP, Oquendo MB, Silva-Filho JL, Takiya CM, Leal-Cardoso JH, Pinheiro AAS, Caruso-Neves C.
Lithium ameliorates tubule-interstitial injury through activation of the mTORC2/protein kinase B pathway.
PLoS One. 2019 Apr 19;14(4):e0215871.
doi: 10.1371/journal.pone.0215871.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the demonstration of macrophages in murine kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Renal pathology, T cells and macrophages infiltration. F, CD3+ T cells and F4/80+ macrophages in IHC staining; Original magnification, 400×.
From: Wu CY, Hua KF, Yang SR, Tsai YS, Yang SM, Hsieh CY, Wu CC, Chang JF, Arbiser JL, Chang CT, Chen A, Ka SM.
Tris DBA ameliorates IgA nephropathy by blunting the activating signal of NLRP3 inflammasome through SIRT1- and SIRT3-mediated autophagy induction.
J Cell Mol Med. 2020 Nov 1 [Epub ahead of print].
doi: 10.1111/jcmm.15663. <.br>This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Biotinylated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497B) used for the demonstration of F4/80 expressing macrophages in mouse tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
After DSS treatment, increased numbers of F4/80-positive cells were observed in the lamina propria mucosae and tela submucosa in all tissue sections. magnification 400×, scale bar: 50 μm.
From: Kerber EL, Padberg C, Koll N, Schuetzhold V, Fandrey J, Winning S.
The Importance of Hypoxia-Inducible Factors (HIF-1 and HIF-2) for the Pathophysiology of Inflammatory Bowel Disease.
Int J Mol Sci. 2020 Nov 13;21(22):8551.
doi: 10.3390/ijms21228551.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti mouse F4/80 antibody, clone Cl:A3-1 (MCA497GA) used for the demonstration of macrophages in murine liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
CU06-1004 has anti-inflammatory effects in mice with CDAA diet-induced NASH.
Immunohistochemical analysis of F4/80 in liver.
From: Bae C-R, Zhang H, Kwon Y-G (2020)
The endothelial dysfunction blocker CU06-1004 ameliorates choline-deficient L-amino acid diet-induced non-alcoholic steatohepatitis in mice.
PLoS ONE 15(12): e0243497.
doi: 10.1371/journal.pone.0243497
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497) used to stain macrophages in murine omental tumor tissue by immuofluorescence.
Image caption:
The doxycycline-inducible IKFM model of NF-κB activation in macrophages. E. Representative images from immunofluorescence analysis of TBR5 omental tumor tissue from FVB IKFM mice showing tumor-infiltrating macrophages; nuclei (blue); F4/80 (green); FLAG-IKK2 (red).
From: Hoover AA, Hufnagel DH, Harris W, Bullock K, Glass EB, Liu E, Barham W, Crispens MA, Khabele D, Giorgio TD, Wilson AJ, Yull FE.
Increased canonical NF-kappaB signaling specifically in macrophages is sufficient to limit tumor progression in syngeneic murine models of ovarian cancer.
BMC Cancer. 2020 Oct 7;20(1):970.
doi: 10.1186/s12885-020-07450-8.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497) used to stain macrophages in murine kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Double deletion of ACE2 and Mas receptor enhances Ang II‐induced renal inflammation. A, F4/80+ macrophages infiltrating the kidney detected by immunohistochemistry. Data represent the mean ± SEM for groups of 6‐12 mice. *P <.05, ***P <.001 vs. WT + SL; # P <.05, ## P <.01, ### P <.001 vs. WT + Ang II; $ P <.05, $$$ P <.001 vs. ACE2 KO + Ang II and Mas KO + Ang II. Scale bar, 50 μm.
From: Ni J, Yang F, Huang XR, Meng J, Chen J, Bader M, Penninger JM, Fung E, Yu XQ, Lan HY.
Dual deficiency of angiotensin-converting enzyme-2 and Mas receptor enhances angiotensin II-induced hypertension and hypertensive nephropathy.
J Cell Mol Med. 2020 Sep 24;24(22):13093–103.
doi: 10.1111/jcmm.15914.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used to demonstrate the presence of macrophages in mouse brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
F4/80 staining of macrophages in E12.5 Csf1r+/+ and Csf1rΔFIRE/ΔFIRE embryos. Representative images of E12.5 Csf1r+/+ and Csf1rΔFIRE/ΔFIRE embryonic heads stained for F4/80. (D-E) Anti-F4/80 stained ChP of the lateral (lat.) and fourth (4th) ventricles. (F-G) F4/80+ macrophages (brown) in the cephalic mesenchyme and developing CNS tissue (these images show magnified versions of the red boxed regions in B-C) Inset boxes in F and G show magnified images of cephalic mesenchyme F4/80+ BAMs (green arrows).
From: Munro DAD, Bradford BM, Mariani SA, Hampton DW, Vink CS, Chandran S, Hume DA, Pridans C, Priller J.
CNS macrophages differentially rely on an intronic Csf1r enhancer for their development.
Development. 2020 Dec 15;147(23):dev194449.
doi: 10.1242/dev.194449.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine renal tissue by immunohistochemistry on cryostat sections.
Image caption:
SSAO inhibition prevents inflammatory cell infiltrates.
F4/80 Staining. Representative photomicrographs of immunohistochemistry for tubulointerstitial F4/80 stain for activated macrophages and histograms summarising expression. Control IgG1 staining was performed to confirm specific staining (not shown). Data presented as mean ± SEM with (*p<0.05, **p<0.01 vs control, #p<0.05 vs DM) n = 6-8. Bar = 200μm DM, Diabetes Mellitus; SSAOi, Semicarbazide Sensitive Amine Oxidase Inhibitor; Telmi, telmisartan.
From: Wong MY, Saad S, Wong MG, Stangenberg S, Jarolimek W, Schilter H, et al. (2020)
Semicarbazide-sensitive amine oxidase inhibition ameliorates albuminuria and glomerulosclerosis but does not improve tubulointerstitial fibrosis in diabetic nephropathy.
PLoS ONE 15(6): e0234617.
doi: 10.1371/journal.pone.0234617
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to evaluate F4/80 expression in murine liver by western blotting and immunohistochemistry on formalin fixed, paraffin embedded tissue sections following antigen retrieval.
Image caption:
Hepatic infiltration of macrophages accompanies a rise in Runx2 and RANKL during NAFLD development. (a) Western blot detected F4/80 protein expression in liver tissues. (b) Real-time PCR detecting the mRNA expression of F4/80 in liver tissues. (c) Liver sections immunohistochemical staining for F4/80; representative images were amplified 200 and 400 times. (d) Quantification for F4/80+ cells presented as a total number of positive cells/field (×200). (e) The correlation between RANKL protein expression and F4/80-positive cells in each sample in the HFD group.
From: Zhong L, Yuan J, Huang L, Li S, Deng L.
RANKL Is Involved in Runx2-Triggered Hepatic Infiltration of Macrophages in Mice with NAFLD Induced by a High-Fat Diet.
Biomed Res Int. 2020 May 25;2020:6953421.
doi: 10.1155/2020/6953421.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used to label macrophages in murine sciatic nerve cryosections by immunofluorescence.
Image caption:
An HSD activates peripheral nerve macrophages.
Longitudinal sections of mouse sciatic nerves were stained with CD16/32 (FcγII/III receptor), F4/80, and DAPI, showing an increased F4/80+ macrophage density in HSD nerves primarily colocalized with CD16/32.
From: Fan A, Oladiran O, Shi XQ, Zhang J.
High-salt diet decreases mechanical thresholds in mice that is mediated by a CCR2-dependent mechanism.
J Neuroinflammation. 2020 Jun 9;17(1):179.
doi: 10.1186/s12974-020-01858-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to identify macrophages in murine liver by immunohistochemistry on formalin fixed, paraffin ebmedded tissue sections.
Image caption:
Pemafibrate improves macrovesicular steatosis and F4/80 positive cell accumulation in STAM mice liver. Representative gross morphology of liver, H&E stained and F4/80 stained liver section from normal, vehicle, and pemafibrate treated STAM mice.
From: Sasaki Y, Asahiyama M, Tanaka T, Yamamoto S, Murakami K, Kamiya W, Matsumura Y, Osawa T, Anai M, Fruchart JC, Aburatani H, Sakai J, Kodama T.
Pemafibrate, a selective PPARα modulator, prevents non-alcoholic steatohepatitis development without reducing the hepatic triglyceride content.
Sci Rep. 2020 May 8;10(1):7818.
doi: 10.1038/s41598-020-64902-8.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R used to identify macrophages in murine
Image caption:
Macrophage marker upregulation in SCs and GERCs during TMEV infection. (a) Under steady-state conditions, F4/80 (green) was weakly expressed in SCs, but not in GERCs. Conversely, the SCs and GERCs migrating to the HCs during the virus infection exhibited strong F4/80 expression (green), indicating the virus-induced activation of SCs and GERCs as macrophages.
From: Hayashi Y, Suzuki H, Nakajima W, Uehara I, Tanimura A, Himeda T, Koike S, Katsuno T, Kitajiri SI, Koyanagi N, Kawaguchi Y, Onomoto K, Kato H, Yoneyama M, Fujita T, Tanaka N.
Cochlear supporting cells function as macrophage-like cells and protect audiosensory receptor hair cells from pathogens.
Sci Rep. 2020 Apr 21;10(1):6740.
doi: 10.1038/s41598-020-63654-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to stain macrophages in formalin fixed paraffin embedded murine liver sections by immunohistochemistry.
Image caption:
Pharmacologic inhibition of ATP signaling by clodronate protects against methionine- and choline-deficient diet-induced steatohepatitis. Ten-week-old C57BL/6 male mice were fed with normal chow (NC) or a methionine- and choline-deficient (MCD) diet for 4 weeks. The vehicle or 20 mg/kg body weight clodronate (Clo) was administered via daily subcutaneous injection for 4 weeks (n = 5–10 MCD diet-fed, per treatment; n = 3–5 NC-fed, per treatment). (A) Hematoxylin and eosin (HE) staining (arrowheads indicate inflammatory foci), F4/80 immunostaining, and Picrosirius Red staining of paraffin-embedded liver tissue from MCD diet-fed mice treated with the vehicle or clodronate. Scale bars, 100 μm.
From: Hasuzawa N, Tatsushima K, Wang L, Kabashima M, Tokubuchi R, Nagayama A, Ashida K, Ogawa Y, Moriyama Y, Nomura M.
Clodronate, an inhibitor of the vesicular nucleotide transporter, ameliorates steatohepatitis and acute liver injury.
Sci Rep. 2021 Mar 4;11(1):5192.
doi: 10.1038/s41598-021-83144-w.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to stain macrophages in formalin fixed paraffin embedded murine liver sections by immunohistochemistry.
Image caption:
Oral administration of clodronate prevents high-fat, high-cholesterol diet-induced steatohepatitis. Ten-week-old C57BL/6 male mice were fed a normal chow (NC) diet or a high-fat, high-cholesterol (HFHC) diet for 24 weeks. The vehicle or clodronate was administered in the drinking water (30 mg/kg body weight/day) for 24 weeks, n = 5 per treatment group. Hematoxylin and eosin (HE) staining (arrowheads indicate inflammatory foci), Picrosirius Red staining, and F4/80 immunostaining of paraffin-embedded mouse liver tissue. Scale bars, 100 μm.
From: Hasuzawa N, Tatsushima K, Wang L, Kabashima M, Tokubuchi R, Nagayama A, Ashida K, Ogawa Y, Moriyama Y, Nomura M.
Clodronate, an inhibitor of the vesicular nucleotide transporter, ameliorates steatohepatitis and acute liver injury.
Sci Rep. 2021 Mar 4;11(1):5192.
doi: 10.1038/s41598-021-83144-w.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to immunostain macrophages in murine liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
S166 phosphorylation is essential for RIPK1-dependent hepatitis and liver tumorigenesis in NEMOLPC-KO mice.
Representative images of liver sections from 8-week-old mice of the indicated genotypes that were immunostained with the indicated antibody.
Scale bars, 200 μm.
From: Laurien L, Nagata M, Schünke H, Delanghe T, Wiederstein JL, Kumari S, Schwarzer R, Corona T, Krüger M, Bertrand MJM, Kondylis V, Pasparakis M.
Autophosphorylation at serine 166 regulates RIP kinase 1-mediated cell death and inflammation.
Nat Commun. 2020 Apr 8;11(1):1747.
doi: 10.1038/s41467-020-15466-8..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to identify macrophages in murine liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Fluorescence multiplex immunohistochemistry (mIHC) of draining LNs harvested at different time points after i.m. immunization. a Representative seven-color multispectral image of a lymph node collected 4 h after i.m. injection of HA-OVA, scanned at ×20 (upper panel) and ×40 (lower panel) magnification. The white arrows in the ×40 image show examples of an OVA+ DC (1, CD11c), OVA+ lymphatic endothelial cell (2, LYVE-1), and OVA+ macrophage (3, F4/80). b Quantification of OVA+ cell density (cells/mm2) in LNs of mice subjected to i.m. injection with 10 μg of OVA alone, mixed with alum, or conjugated to HA. c Percentage of OVA+ cells among LYVE-1+, CD11c+, and F4/80+ cells. Cumulative data from three independent experiments are presented (n = 3 mice; 6 LNs/group). Multiple t-tests were performed (*P <0.05, **P <0.01, ***P <0.001, ****P <0.0001; P >0.05 if not indicated).
From: Dalla Pietà A, Carpanese D, Grigoletto A, Tosi A, Dalla Santa S, Pedersen GK, Christensen D, Meléndez-Alafort L, Barbieri V, De Benedictis P, Pasut G, Montagner IM, Rosato A.
Hyaluronan is a natural and effective immunological adjuvant for protein-based vaccines.
Cell Mol Immunol. 2021 Mar 24.
doi: 10.1038/s41423-021-00667-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used to label macrophages in murine intestinal tissue sections by immunofluorescence.
Image caption:
Expression of resolvin D1 receptor, lipoxin A4 receptor /formyl peptide receptor 2 (ALX/FPR2) in the small intestine and its dynamics on indomethacin-induced small intestinal damage.
(b) Relative amount of normalized ALX/FPR2 protein expressed as a percentage of untreated control group (0 h group). *p <0.05 vs. 0 h group. N = 4. (c) Representative image of immunofluorescent staining for localization analysis of ALX/FPR2 in damaged small intestinal mucosa. DAPI: 4’,6-diamidino-2-phenylindole. Bars in histological images: 50 μm. (d) Expression of ALX/FPR2 and its colocalization with a marker of mature macrophages F4/80. These are enlarged views of the part indicated by white squares in image.
From: Kuzumoto T, Tanigawa T, Higashimori A, Kitamura H, Nadatani Y, Otani K, et al. (2021)
Protective role of resolvin D1, a pro-resolving lipid mediator, in nonsteroidal anti-inflammatory drug-induced small intestinal damage.
PLoS ONE 16(5): e0250862.
doi: 10.1371/journal.pone.0250862
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to identify macrophages in murine tumor tissues.
Image caption:
Evaluation of immune microenvironment in parental-, clone-1-, clone-16-derived primary tumor and liver metastases. Immunofluorescence imaging of (a) CD8, (c) CD45, and (e) F480 in both primary and liver metastases. Scale bar, 100 μm.
From: Liu YT, Goel S, Kai M, Moran Guerrero JA, Nguyen T, Mai J, Shen H, Ziemys A, Yokoi K.
Seed- and Soil-Dependent Differences in Murine Breast Tumor Microenvironments Dictate Anti-PD-L1 IgG Delivery and Therapeutic Efficacy.
Pharmaceutics. 2021 Apr 10;13(4):530.
doi: 10.3390/pharmaceutics13040530.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to identify macrophages in murine tumor tissues.
Image caption:
Verification of immune maker that express PD-L1 by immunohistochemical imaging of primary tumor and liver metastases. Immunofluorescence imaging of PD-L1 expression (red) and F4/80 (green) in parental- primary tumors and liver metastases. Scale bar, 100μm
From: Liu YT, Goel S, Kai M, Moran Guerrero JA, Nguyen T, Mai J, Shen H, Ziemys A, Yokoi K.
Seed- and Soil-Dependent Differences in Murine Breast Tumor Microenvironments Dictate Anti-PD-L1 IgG Delivery and Therapeutic Efficacy.
Pharmaceutics. 2021 Apr 10;13(4):530.
doi: 10.3390/pharmaceutics13040530.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine pancreas by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Fibrosis and chronic inflammation in the Cby1-KO pancreas.
Pancreatic sections from adult mice were immunostained with antibodies for the inflammation markers CD45 (leukocytes) and F4/80 (macrophages). Scale bars: CD45, 100 μm; F4/80, 50 μm.
From: Cyge B, Voronina V, Hoque M, Kim EN, Hall J, Bailey-Lundberg JM, Pazour GJ, Crawford HC, Moon RT, Li FQ, Takemaru KI.
Loss of the ciliary protein Chibby1 in mice leads to exocrine pancreatic degeneration and pancreatitis.
Sci Rep. 2021 Aug 26;11(1):17220.
doi: 10.1038/s41598-021-96597-w.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine intestine by immunofluorescence.
Image caption:
Representative immunofluorescence images of macrophages (F4/80+) in the small intestinal wall. C57BL/6 N mice underwent CASP following SID or a sham operation. (A) Aboral 1 cm of ileum was cryofixed and sections were stained for infiltrating macrophages using antibody against F4/80-antigen labelled with Alexa-647 dye and DAPI for staining of cell nuclei. Photos were taken from at least 6 mice per group. (Magnification 20x) (B) Fluorescence was assigned to the mucosal region and outer layers of muscularis and serosa (see materials and methods for detail). F4/80 positive events in (B1) whole cross section of small intestine, (B2) mucosa and (B3) muscularis were compared between operated groups. N = 6 to 8 (technical failures have been excluded); Mann–Whitney U-Test; **p <0.01.
From: Menz J, Hundt L, Schulze T, Schmoeckel K, Menges P, Domanska G.
Increased mortality and altered local immune response in secondary peritonitis after previous visceral operations in mice.
Sci Rep. 2021 Aug 10;11(1):16175.
doi: 10.1038/s41598-021-95592-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497) used to detect F4/80 expressing macrophages in murine renal tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Latent TGF-β1 attenuates renal fibrosis and inflammation in streptozotocin-induced type 1 diabetic mice. Mice were euthanized 16 weeks after diabetes induction, and renal tissues were collected. (C) Immunohistochemistry of F4/80-positive cells showing macrophage infiltration in glomeruli and tubulointerstitium. DM, diabetes mellitus. WT, latent TGF-β1 wild-type mice. Tg, latent TGF-β1 transgenic mice. Data represent the means ± SEM for groups of six animals. Scale bar: 50 μm. *P <0.05, **P <0.01, ***P <0.001 versus normal mice; #P <0.05, ##P <0.01, ###P <0.001 versus WT DM mice.
From: Wu W, Huang XR, You Y, Xue L, Wang XJ, Meng X, Lin X, Shen J, Yu X, Lan HY, Chen H.
Latent TGF-β1 protects against diabetic kidney disease via Arkadia/Smad7 signaling.
Int J Biol Sci. 2021 Aug 19;17(13):3583-94.
doi: 10.7150/ijbs.61647.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine kidneys by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Deficiency of myeloid TLR4 inhibits macrophage-dominant renal infiltrates in experimental anti-GBM GN. A Representative images of neutrophil (NIMP-R14+) and macrophage (F4/80+) accumulation in kidney sections (magnification × 40).
From: Yang F, Chen J, Huang XR, Yiu WH, Yu X, Tang SCW, Lan HY.
Regulatory role and mechanisms of myeloid TLR4 in anti-GBM glomerulonephritis.
Cell Mol Life Sci. 2021 Sep 27 [Epub ahead of print].
doi: 10.1007/s00018-021-03936-1. .
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to detect macrophages in mouse skin by immunofluorescence.
Image caption:
Representative images of skin sections from 3-week-old mice of the indicated genotypes, immunostained with antibodies against keratin 10 (K10), keratin 14 (K14) (n = 6), keratin 6 (K6) (n = 6), F4/80 (n = 6) and Gr-1 (n = 6) and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 μm; F4/80, Gr-1 = 30 μm.
From: Schünke H, Göbel U, Dikic I, Pasparakis M.
OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice.
Nat Commun. 2021 Oct 8;12(1):5912.
doi: 10.1038/s41467-021-25945-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to detect macrophages in mouse skin by immunofluorescence.
Image caption:
Representative images of skin sections from 3-week-old mice of the indicated genotypes, immunostained with anti-K10, anti-K14 (n = 5), anti-K6 (n = 5), anti-F4/80 (n = 3) and anti-Gr-1 (n = 3) antibodies and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 μm; F4/80, Gr-1 = 30 μm.
From: Schünke H, Göbel U, Dikic I, Pasparakis M.
OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice.
Nat Commun. 2021 Oct 8;12(1):5912.
doi: 10.1038/s41467-021-25945-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to detect macrophages in mouse skin by immunofluorescence.
Image caption:
Representative images of skin sections from 3-week-old mice of the indicated genotypes, immunostained with anti-K10, anti-K14 (n = 4), anti-K6 (n = 3), anti-F4/80 (n = 3) and anti-Gr-1 (n = 3) antibodies and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 μm; F4/80, Gr-1 = 30 μm.
From: Schünke H, Göbel U, Dikic I, Pasparakis M.
OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice.
Nat Commun. 2021 Oct 8;12(1):5912.
doi: 10.1038/s41467-021-25945-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to detect macrophages in mouse skin by immunofluorescence.
Image caption:
Representative images from skin sections from 3-week-old mice of the indicated genotypes, immunostained with anti-K10, anti-K14 (n = 5), anti-K6 (n = 5), anti-F4/80 (n = 5) and anti-Gr-1 (n = 5) antibodies and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 μm; F4/80, Gr-1 = 30 μm.
From: Schünke H, Göbel U, Dikic I, Pasparakis M.
OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice.
Nat Commun. 2021 Oct 8;12(1):5912.
doi: 10.1038/s41467-021-25945-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to detect macrophages in mouse skin by immunofluorescence.
Image caption:
Representative images from skin sections from 3-week-old mice of the indicated genotypes, immunostained with anti-K10, anti-K14 (n = 4), anti-K6 (n = 3), anti-F4/80 (n = 3) and anti-Gr-1 (n = 3) antibodies and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 μm; F4/80, Gr-1 = 30 μm.
From: Schünke H, Göbel U, Dikic I, Pasparakis M.
OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice.
Nat Commun. 2021 Oct 8;12(1):5912.
doi: 10.1038/s41467-021-25945-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Circulating FGF-2-induced tubulo-interstitial changes in HIV-Tg26 mice and mimic those seen in childhood HIVAN.
Representative immunohistochemistry staining for PCNA+, vimentin+ (both brown), α-smooth muscle actin+ (α-SMA+) and F4/80+ macrophages (both red) in renal sections harvested from WT and HIV-Tg26 mice 14 days after the injections of rAd-LacZ (control) or rAd-FGF-2 vectors (n=3-4 mice per group). Scale bar: 20 μ m.
From: Das JR, Jerebtsova M, Tang P, Li J, Yu J, Ray PE.
Circulating fibroblast growth factor-2 precipitates HIV nephropathy in mice.
Dis Model Mech. 2021 Jul 1;14(7):dmm048980.
doi: 10.1242/dmm.048980.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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Image caption:
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From: Fukase M, Sakata N, Kushida Y, Wakao S, Unno M, Dezawa M. Intravenous injection of human multilineage-differentiating stress-enduring cells alleviates mouse severe acute pancreatitis without immunosuppressants. Surg Today. 2021 Oct 23. doi: 10.1007/s00595-021-02382-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to identify macrophages in murine renal tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
BHD reduces renal fibrosis and inflammation in STZ-induced diabetic mice. B Immunohistochemistry of F4/80-positive cells showing macrophage infiltration in glomeruli and tubulointerstitium. DM, diabetes mellitus. Irbesartan (IRB) was taken as a positive control. Data represent the means ± SEM for groups of six animals. Scale bar: 50μm. *P <0.05, **P <0.01, ***P <0.001 versus normal mice. #P <0.05, ##P <0.01, ###P <0.001 versus DM mice.
From: Wu W, Wang Y, Li H, Chen H, Shen J.
Buyang Huanwu Decoction protects against STZ-induced diabetic nephropathy by inhibiting TGF-β/Smad3 signaling-mediated renal fibrosis and inflammation.
Chin Med. 2021 Nov 14;16(1):118.
doi: 10.1186/s13020-021-00531-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti mouse F4/80 antibody, clone A3-1 (MCA497G) used to identify macrophages in murine renal tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Frequencies of intra-renal CD4, CD8, F4/80 and B220 positive cells in the kidneys of 19-week-old female MRL-FaslprKSRP−/− mice compared to MRL-FaslprKSRP+/+ mice Immunostaining was used to analyze intra-renal frequency of CD4, CD8, F4/80 and B220 positive cells in the kidneys of female 19-week-old MRL-FaslprKSRP+/+ and MRL-FaslprKSRP−/− mice. Data presented are the mean + SEM of the score with 8 to 12 animals per genotype (*** p <0.001 compared to WT, t-test). Representative image of immunostaining with 40× magnification.
From: Schmidtke, L.; Meineck, M.; Saurin, S.; Otten, S.; Gather, F.; Schrick, K.; Käfer, R.; Roth, W.; Kleinert, H.; Weinmann-Menke, J.; Pautz, A.
Knockout of the KH-Type Splicing Regulatory Protein Drives Glomerulonephritis in MRL-Faslpr Mice.
Cells 2021, 10, 3167.
10.3390/cells10113167
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in atherosclerotic plaques by immunohistochemistry on formalin fixed cryosections.
Image caption:
Representative photomicrographs of anti-F4/80 immunohistochemistry of cross-sections at the brachiocephalic artery from 6 groups of SD-PBS (n = 4), SD-αGC (n = 6), SD-KO (n = 3), HFD-PBS (n = 18), HFD-αGC (n = 16), and HFD-KO (n = 7) mice. Scale bar = 100 μm.
From: Ohmura Y, Ishimori N, Saito A, Yokota T, Horii S, Tokuhara S, Iwabuchi K, Tsutsui H.
Natural Killer T Cells Are Involved in Atherosclerotic Plaque Instability in Apolipoprotein-E Knockout Mice.
Int J Mol Sci. 2021 Nov 18;22(22):12451.
doi: 10.3390/ijms222212451.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti mouse F4/80 antibody, clone A3-1 (MCA497R) used to visualize macrophages in murine hepatic tissues by immunofluorescence on cryosections.
Image caption:
Representative IF microphotographs of 8 to 72 week-old Jnkf/f and JnkΔhepa mice for F4/80. Scale bar, 200 μm.
From: Chen C, Wu H, Ye H, Tortajada A, Rodríguez-Perales S, Torres-Ruiz R, Vidal A, Peligros MI, Reissing J, Bruns T, Mohamed MR, Zheng K, Lujambio A, Iraburu MJ, Colyn L, Latasa MU, Arechederra M, Fernández-Barrena MG, Berasain C, Vaquero J, Bañares R, Nelson LJ, Trautwein C, Davis RJ, Martinez-Naves E, Nevzorova YA, Villanueva A, Avila MA, Cubero FJ.
Activation of the Unfolded Protein Response (UPR) Is Associated with Cholangiocellular Injury, Fibrosis and Carcinogenesis in an Experimental Model of Fibropolycystic Liver Disease.
Cancers. 2022; 14(1):78.
doi: 10.3390/cancers14010078
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine bone marrow by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
Effects of PAI-1 deficiency on Dex-induced decreases in macrophage number after femoral bone injury.
(A) Microphotographs of F4/80-positive cells at damaged sites 2 days after femoral bone injury in control and Dex-treated WT and PAI-1 KO mice. Scale bars indicate 50μm. (B) Number of F4/80-positive cells at damaged sites 2 and 4 days after femoral bone injury in control and Dex-treated WT and PAI-1 KO mice. Data represent the mean ± SEM: n = 5 mice in each group. * p <0.05.
Cont, control; DAPI, 4′,6-diamidino-2-phenylindole.
From: Okada, K.; Kawao, N.; Nakai, D.; Wakabayashi, R.; Horiuchi, Y.; Okumoto, K.; Kurashimo, S.; Takafuji, Y.; Matsuo, O.; Kaji, H.
Role of Macrophages and Plasminogen Activator Inhibitor-1 in Delayed Bone Repair Induced by Glucocorticoids in Mice
Int. J Mol Sci 2022, 23, 478.
doi: 10.3390/ijms23010478
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
R-phycoerythrin conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497PE) used to label macrophages in murine bone marrow by Flow cytometry.
Image caption:
Effects of Dex and PAI-1 deficiency on the expression of macrophage-related factors in macrophages derived from the bone marrow of damaged femurs.
Flow cutometric analysis was used to identify F4/80 and CD11b double-positive cells from damaged femurs 2 days after bone injury.
From: Okada, K.; Kawao, N.; Nakai, D.; Wakabayashi, R.; Horiuchi, Y.; Okumoto, K.; Kurashimo, S.; Takafuji, Y.; Matsuo, O.; Kaji, H.
Role of Macrophages and Plasminogen Activator Inhibitor-1 in Delayed Bone Repair Induced by Glucocorticoids in Mice
Int. J Mol Sci 2022, 23, 478.
doi: 10.3390/ijms23010478
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections and by western blotting on hepatic lysates.
Image caption:
Depletion of Kupffer cells enhances Efavirenz-induced liver injury in vivo. (A–C) Confirmation of macrophage depletion in mice administered with gadolinium chloride (GdCl3): relative mRNA expression levels of F4/80 (RT-PCR), representative images showing F4/80 immunostaining in murine livers (magnification 20×), and representative Western Blot image of F4/80 (each blot lane represents one mouse per condition—saline and GdCl3−) and summary of densitometry data (n = 6).
From: Alegre, F.; Martí-Rodrigo, A.; Polo, M.; Ortiz-Masiá, D.; Bañuls, C.; Pinti, M.; Álvarez, Á.; Apostolova, N.; Esplugues, J.V.; Blas-García, A.
Macrophages Modulate Hepatic Injury Involving NLRP3 Inflammasome: The Example of Efavirenz.
Biomedicines 2022, 10, 109.
doi: 10.3390/biomedicines10010109
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine umor sections by immunofluorescence.
Image caption:
MAG-OV+MT stimulates a T lymphocyte-mediated antitumor response. Representative images of EO771 tumor sections examined by terminal immunofluorescent staining and their quantification of signals for macrophages (F4/80). Data are shown as mean ± SD (n = 4). Statistical significance was determined by one-way ANOVA with a Tukey post hoc test.
From: Howard FHN, Al-Janabi H, Patel P, Cox K, Smith E, Vadakekolathu J, Pockley AG, Conner J, Nohl JF, Allwood DA, Collado-Rojas C, Kennerley A, Staniland S, Muthana M.
Nanobugs as Drugs: Bacterial Derived Nanomagnets Enhance Tumor Targeting and Oncolytic Activity of HSV-1 Virus.
Small. 2022 Jan 25:e2104763.
doi: 10.1002/smll.202104763.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
alexa Fluor 647 conjugated Rat anti mouse F4/80 antibody, clone A3-1 (MCA497A647) used to label murine spinal cord macrophages by immubofluorescence.
Image caption:
Immunofluorescence staining for tdTomato (red), CD11b (yellow), F4/80 (cyan), MHCII (blue) and MerTK (green) of spinal cord from mice at peak EAE. A representative picture of three individual mouse samples is shown (n = 3); scale bar, 50 μm (low magnification) and 10 μm (inset).
From: Sarkar T, Patro N, Patro IK.
Perinatal exposure to synergistic multiple stressors lead to cellular and behavioral deficits mimicking Schizophrenia like pathology.
Biol Open. 2022 Feb 2:bio.058870.
doi: 10.1242/bio.058870.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to identify infiltrating macrophages in mouse renal tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Notch3 overexpression results in severe tubulointerstitial inflammation and fibrosis. F4/80 immunostaining was used for evaluation of infiltrating macrophages, α-smooth muscle actin (αSMA) for fibroblast activation.
From: Djudjaj S, Kavvadas P, Prakoura N, Bülow RD, Migeon T, Placier S, Chadjichristos CE, Boor P, Chatziantoniou C.
Activation of Notch3 in Renal Tubular Cells Leads to Progressive Cystic Kidney Disease. Int J Mol Sci. 2022 Jan 14;23(2):884.
doi: 10.3390/ijms23020884.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine pulmonary tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
A–C Representative images (on the left) and quantifications (on the right) showing F4/80+ cells staining positive for A pSTAT3, B PPARγ, C c-MYC immunostainings in alveolar parenchyma and adenoma sections from BRAFV600E and control mice.
Quantifications were performed on at least five different areas of the lung sections in a random way. Data are expressed as mean ± SEM; n = animals per group. *P <0.05; **P <0.01; ***P <0.001, ns = not significant. (ANOVA test with Dunnet post-hoc correction (A, B, C)). Arrows point to selected positive cells for the indicated marker. Insets: magnifications of areas inside dashed squares.
From: Bosso G, Lanuza-Gracia P, Piñeiro-Hermida S, Yilmaz M, Serrano R, Blasco MA.
Early differential responses elicited by BRAFV600E in adult mouse models.
Cell Death Dis. 2022 Feb 10;13(2):142.
doi: 10.1038/s41419-022-04597-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine pulmonary tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
D–F Representative images (top) and quantifications (bottom) showing F4/80+ cells staining positive for D pSTAT3, E PPARγ, F c-MYC double immunostainings in bronchi/bronchiolar parenchyma of lung sections from BRAFV600E and control mice.
Quantifications were performed on at least five different areas of the lung sections in a random way. Data are expressed as mean ± SEM; n = animals per group. *P <0.05; **P <0.01; ***P <0.001, ns = not significant. (ANOVA test with T Student’s test unpaired (D, E, F)). Arrows point to selected positive cells for the indicated marker. Insets: magnifications of areas inside dashed squares.
From: Bosso G, Lanuza-Gracia P, Piñeiro-Hermida S, Yilmaz M, Serrano R, Blasco MA.
Early differential responses elicited by BRAFV600E in adult mouse models.
Cell Death Dis. 2022 Feb 10;13(2):142.
doi: 10.1038/s41419-022-04597-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine pulmonary tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Ubiquitous expression of the oncogenic variant of BRAF results in neutrophils infiltration in alveoli and F4/80-positive cells infiltration in bronchial epithelium as well as in hyperplastic nodules.
Representative images (top) and quantifications (bottom) showing H/E, Myeloperoxidase (MPO), F4/80, and CD4 immunostainings in alveoli (A), (B), (C), (D), from BRAFV600E and control mice. Quantifications in alveoli and bronchi were performed on at least five different areas of the lung sections in a random way. Data are expressed as mean ± SEM; n= animals per group, * P<0.05; ** P<0.01; *** P<0.001, ns=not significant. (T Student’s test unpaired). Arrows point to selected positive cells for the indicated marker. Insets: magnifications of areas inside dashed squares.
From: Bosso G, Lanuza-Gracia P, Piñeiro-Hermida S, Yilmaz M, Serrano R, Blasco MA.
Early differential responses elicited by BRAFV600E in adult mouse models.
Cell Death Dis. 2022 Feb 10;13(2):142.
doi: 10.1038/s41419-022-04597-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine pulmonary tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Ubiquitous expression of the oncogenic variant of BRAF results in neutrophils infiltration in alveoli and F4/80-positive cells infiltration in bronchial epithelium as well as in hyperplastic nodules.
Representative images (top) and quantifications (bottom) showing H/E, Myeloperoxidase (MPO), F4/80, and CD4 immunostainings in alveoli (A), (B), (C), (D), from BRAFV600E and control mice. Quantifications in alveoli and bronchi were performed on at least five different areas of the lung sections in a random way. Data are expressed as mean ± SEM; n= animals per group, * P<0.05; ** P<0.01; *** P<0.001, ns=not significant. (T Student’s test unpaired). Arrows point to selected positive cells for the indicated marker. Insets: magnifications of areas inside dashed squares.
From: Bosso G, Lanuza-Gracia P, Piñeiro-Hermida S, Yilmaz M, Serrano R, Blasco MA.
Early differential responses elicited by BRAFV600E in adult mouse models.
Cell Death Dis. 2022 Feb 10;13(2):142.
doi: 10.1038/s41419-022-04597-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine pulmonary tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Ubiquitous expression of the oncogenic variant of BRAF results in neutrophils infiltration in bronchi and F4/80-positive cells infiltration in bronchial epithelium as well as in hyperplastic nodules.
Representative images (top) and quantifications (bottom) showing H/E, Myeloperoxidase (MPO), F4/80, and CD4 immunostainings in in bronchi respectively (E) F4/80, (F) MPO and (G) CD4 from BRAFV600E and control mice. Quantifications in alveoli and bronchi were performed on at least five different areas of the lung sections in a random way. Data are expressed as mean ± SEM; n= animals per group, * P<0.05; ** P<0.01; *** P<0.001, ns=not significant. (T Student’s test unpaired). Arrows point to selected positive cells for the indicated marker. Insets: magnifications of areas inside dashed squares.
From: Bosso G, Lanuza-Gracia P, Piñeiro-Hermida S, Yilmaz M, Serrano R, Blasco MA.
Early differential responses elicited by BRAFV600E in adult mouse models.
Cell Death Dis. 2022 Feb 10;13(2):142.
doi: 10.1038/s41419-022-04597-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine pulmonary tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Ubiquitous expression of the oncogenic variant of BRAF results in neutrophils infiltration in bronchi and F4/80-positive cells infiltration in bronchial epithelium as well as in hyperplastic nodules.
Representative images (top) and quantifications (bottom) showing H/E, Myeloperoxidase (MPO), F4/80, and CD4 immunostainings in in bronchi respectively (E) F4/80, (F) MPO and (G) CD4 from BRAFV600E and control mice Arrows point to selected positive cells for the indicated marker. Insets: magnifications of areas inside dashed squares.
From: Bosso G, Lanuza-Gracia P, Piñeiro-Hermida S, Yilmaz M, Serrano R, Blasco MA.
Early differential responses elicited by BRAFV600E in adult mouse models.
Cell Death Dis. 2022 Feb 10;13(2):142.
doi: 10.1038/s41419-022-04597-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to identify macrophages in murine adipose tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Acute endotoxemia affects adipose tissue but does not lead to weight gain in mice under chow diet. Swiss Webster mice were submitted to intraperitoneal administration of 8 consecutive doses of 10 mg/kg LPS, every 3 days. Histological sections of epididymal fat pads after LPS challenges showing adipocyte morphology on hematoxylin and eosin staining and macrophage infiltration (F4/80+).
From: Mendes de Oliveira, E.; Silva, J.C.; Ascar, T.P.; Sandri, S.; Marchi, A.F.; Migliorini, S.; Nakaya, H.T.I.; Fock, R.A.; Campa, A.
Acute Inflammation Is a Predisposing Factor for Weight Gain and Insulin Resistance.
Pharmaceutics 2022, 14, 623.
doi:10.3390/pharmaceutics14030623
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to label macrophages in murine renal tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Presence of renal mononuclear phagocytes and expression of inflammasome component in kidney cortex. Expression of markers of mononuclear phagocytes were compared between wild-type (C57BL/6J) and S1P5 knockout mice (S1P5-/-) without (healthy) and with adenine-rich diet for 7 and 14 days. (A) Paraffin-embedded kidney cortex sections were subjected to immunohistochemical staining for F4/80 expression with DAB (brown) and counterstained with Mayer’s hematoxylin solution (blue). Algorithm-based detection of the staining was used to visualize and quantify the area covered by diaminobenzidine (green) and hematoxylin (red). (B) Median DAB area/hematoxylin area ratio was determined for up to 30 single pictures per tissue section, and the medians of all sections per group are shown here. (C,D) Expression levels of Nlrp3 and Adgre1 (also known as Emr1) mRNA were determined by TaqMan® analysis as described in Materials and Methods. The internal standard used was 18s ribosomal RNA. Data are shown as box plots with a line at the median and whiskers from minimum to maximum value; n = 5–9; * p ≤0.05 as indicated; # p ≤0.05; ## p ≤0.01; ### p ≤0.001 compared to the respective healthy controls.
From: Eckes T, Patyna S, Koch A, Oftring A, Gauer S, Obermüller N, Schwalm S, Schaefer L, Chun J, Gröne HJ, Pfeilschifter J.
Sphingosine 1-Phosphate Receptor 5 (S1P5) Knockout Ameliorates Adenine-Induced Nephropathy.
Int J Mol Sci. 2022 Apr 2;23(7):3952.
doi: 10.3390/ijms23073952.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F C P IF WB ResetRat anti Mouse F4/80
- Product Type
- Monoclonal Antibody
- Clone
- Cl:A3-1
- Isotype
- IgG2b
- Specificity
- F4/80
Quick Links:
Antibody Quality and 
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| Rat anti Mouse F4/80 antibody, clone A3-1 recognizes the murine F4/80 antigen, a ~160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al. 1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al. 1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin, et al. 2005) Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999). A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1. |
Our F4/80 (Cl:A3-1) Antibody has been referenced in >719 publications* *Based on June 2020 data from CiteAb's antibody search engine. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- MCA497R, MCA497RT, MCA497GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- MCA497G: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immuno-electron Microscopy | |
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| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 1 | |
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| Immunohistology - Resin | |
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| Immunoprecipitation | |
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| Radioimmunoassays | |
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| Western Blotting | |
- 1 Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
Source Reference
-
Austyn, J.M. & Gordon, S. (1981) F4/80, a monoclonal antibody directed specifically against the mouse macrophage.
Eur J Immunol. 11 (10): 805-15.
Antibody Characterization Reference
-
Hume, D.A. et al. (1984) The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia.
Anat Rec. 210 (3): 503-12. Anat Rec. 210 (3): 503-12. -
Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.
J Exp Med. 161 (3): 475-89. J Exp Med. 161 (3): 475-89.
References for F4/80 antibody
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Mol Imaging Biol. 13: 462-70. -
Bevaart, L. et al. (2004) CpG oligodeoxynucleotides enhance FcgammaRI-mediated cross presentation by dendritic cells.
Int Immunol. 16: 1091-8.
View The Latest Product References
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J Exp Med. 201 (10): 1615-25. -
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Dandekar, A.A.et al. (2004) Bystander CD8 T-cell-mediated demyelination is interferon-gamma-dependent in a coronavirus model of multiple sclerosis.
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- RRID
- AB_872005
- UniProt
- Q61549
- Entrez Gene
- Emr1
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0004930 G-protein coupled receptor activity
- GO:0007218 neuropeptide signaling pathway
- GO:0009897 external side of plasma membrane
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