F4/80 antibody | Cl:A3-1
























































Rat anti Mouse F4/80:Alexa Fluor® 488
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Alexa Fluor® 647
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:APC
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Biotin
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:FITC
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Low Endotoxin
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Pacific Blue®
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:RPE
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:RPE-Alexa Fluor® 647
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:RPE-Alexa Fluor® 750
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Alexa Fluor® 700
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Starbright Blue 700
Rat anti Mouse F4/80:Amethyst Orange
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Starbright Violet 440
Rat anti Mouse F4/80:Starbright Violet 515
Rat anti Mouse F4/80:Starbright Violet 610
Rat anti Mouse F4/80:Starbright Violet 670
- Product Type
- Monoclonal Antibody
- Clone
- Cl:A3-1
- Isotype
- IgG2b

Product Code | Applications | Pack Size | List Price | Quantity |
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MCA497A488T | F | 0.25 ml |
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MCA497A488 | F | 100 Tests/1ml |
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MCA497A647T | F | 25 Tests/0.25ml |
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MCA497A647 | F | 100 Tests/1ml |
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MCA497APC | F | 100 Tests |
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MCA497APCT | F | 25 Tests/0.25ml |
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MCA497BT | F | 25 µg |
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MCA497B | F | 0.1 mg |
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MCA497BB | F | 0.5 mg |
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MCA497FT | F IF | 25 µg |
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MCA497FA | F IF | 50 µg |
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MCA497F | F IF | 0.1 mg |
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MCA497FB | F IF | 0.5 mg |
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MCA497EL | C F FN IP P* R RE WB | 0.5 mg |
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MCA497PBT | F | 25 Tests/0.25ml |
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MCA497PB | F | 100 Tests/1ml |
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MCA497RT | C EM F IF IP P* R RE WB | 25 µg |
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MCA497GA | C EM F IF IP P* R RE WB | 0.1 mg |
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MCA497R | C EM F IF IP P* R RE WB | 0.25 mg |
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MCA497G | C EM F IF IP P* R RE WB | 0.5 mg |
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MCA497PE | F | 100 Tests |
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MCA497PET | F IF | 25 Tests |
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MCA497P647 | F | 100 Tests/1ml |
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MCA497P750 | F | 100 Tests/1ml |
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MCA497 | C EM F IF IP P* R RE | 2 ml |
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MCA497A700T | F | 25 Tests/0.25ml |
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MCA497A700 | F | 100 Tests/1ml |
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MCA497SBB700 | F | 100 Tests/0.5ml |
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MCA497AMO | F | 0.1 mg |
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MCA497SBV440 | F | 100 Tests/0.5ml |
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MCA497SBV515 | F | 100 Tests/0.5ml |
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MCA497SBV610 | F | 100 Tests/0.5ml |
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MCA497SBV670 | F | 100 Tests/0.5ml |
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Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al. 1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al. 1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin, et al. 2005)
Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999).
A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE)-Alexa Fluor® 647 - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE)-Alexa Fluor® 750 - lyophilized
- Product Form
- Tissue Culture Supernatant - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Product Form
- Purified IgG conjugated to StarBright Blue 700 - liquid
- Product Form
- Purified IgG conjugated to Amethyst Orange - liquid
- Product Form
- Purified IgG conjugated to StarBright Violet 440 - liquid
- Product Form
- Purified IgG conjugated to StarBright Violet 515 - liquid
- Product Form
- Purified IgG conjugated to StarBright Violet 610 - liquid
- Product Form
- Purified IgG conjugated to StarBright Violet 670 - liquid
- Reconstitution
- Pack Size: 100 TestsReconstitute with 1 ml distilled waterPack Size: 25 Tests/0.25mlReconstitute with 0.25ml distilled water
- Reconstitution
- Pack Size: 100 TestsReconstitute with 1 ml distilled waterPack Size: 25 TestsReconstitute with 0.25 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- None present
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350 - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350 - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350 - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350 - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- Pack Size: 25 µg, 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- Pack Size: 25 µg, 50 µg, 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 µg, 0.1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 µg, 50 µg, 0.1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 TestsPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q61549
- Entrez Gene
- Emr1
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0004930 G-protein coupled receptor activity
- GO:0007218 neuropeptide signaling pathway
- GO:0009897 external side of plasma membrane
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Regulatory
- For research purposes only
Applications of F4/80 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat Pack Size: 25 µg, 0.1 mg 1/10 Pack Size: 0.5 mg |
1/10 Pack Size: 25 µg, 0.1 mg 1/50 Pack Size: 0.5 mg |
|
Flow Cytometry | Neat Pack Size: 50 µg 1/10 Pack Size: 0.5 mg |
Neat Pack Size: 25 µg, 0.1 mg 1/10 Pack Size: 50 µg 1/50 Pack Size: 0.5 mg |
|
Immunofluorescence | |||
Flow Cytometry | 1/100 | 1/200 | |
Functional Assays | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | |||
Immunohistology - Resin | |||
Immunoprecipitation | |||
Radioimmunoassays | |||
Western Blotting | |||
Flow Cytometry | Neat | 1/5 | |
Flow Cytometry | 1/50 | 1/100 | |
Immuno-electron Microscopy | |||
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | |||
Immunohistology - Resin | |||
Immunoprecipitation | |||
Radioimmunoassays | |||
Western Blotting | |||
Flow Cytometry | Neat | ||
Immunofluorescence | |||
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | 1/10 | |
Immuno-electron Microscopy | |||
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | |||
Immunohistology - Resin | |||
Immunoprecipitation | |||
Radioimmunoassays | |||
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | ||
Flow Cytometry | Neat | ||
Flow Cytometry | Neat |
- 1 Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.
- 1 Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.
- 1 Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Streptavidin:RPE | STAR4A | F | 1 ml |
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Streptavidin:APC | STAR119 | F | 100 Tests |
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Goat anti Rat IgG (H/L):HRP | 305005 | C E IB | 1 ml |
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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Goat anti Rat IgG2b:HRP | STAR114P | C E P WB | 0.5 mg |
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Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Goat anti Rat IgG (H/L):HRP | 305005 | C E IB | 1 ml |
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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|
Goat anti Rat IgG2b:HRP | STAR114P | C E P WB | 0.5 mg |
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|
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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|
Goat anti Rat IgG (H/L):HRP | 305005 | C E IB | 1 ml |
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|
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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|
Goat anti Rat IgG2b:HRP | STAR114P | C E P WB | 0.5 mg |
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|
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat IgG2b Negative Control:RPE-Alexa Fluor® 750 | MCA6006P750 | F | 100 Tests/1ml |
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Useful Reagents Available
Application Based External Images
Flow Cytometry
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Product Specific References
Source Reference
-
Austyn, J.M. & Gordon, S. (1981) F4/80, a monoclonal antibody directed specifically against the mouse macrophage.
Eur J Immunol. 11 (10): 805-15.
Antibody Characterization Reference
-
Hume, D.A. et al. (1984) The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia.
Anat Rec. 210 (3): 503-12. -
Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.
J Exp Med. 161 (3): 475-89.
References for F4/80 antibody
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Sci Rep. 7 (1): 8801. -
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Otobe, S. et al. (2020) CX3CR1 Deficiency Attenuates DNFB-Induced Contact Hypersensitivity Through Skewed Polarization Towards M2 Phenotype in Macrophages.
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Wu, C.Y. et al. (2020) Tris DBA ameliorates IgA nephropathy by blunting the activating signal of NLRP3 inflammasome through SIRT1- and SIRT3-mediated autophagy induction.
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Kerber, E.L. et al. (2020) The Importance of Hypoxia-Inducible Factors (HIF-1 and HIF-2) for the Pathophysiology of Inflammatory Bowel Disease.
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Lai, K. et al. (2020) Triptolide attenuates laser-induced choroidal neovascularization via M2 macrophage in a mouse model.
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Munro, D.A.D. et al. (2020) CNS macrophages differentially rely on an intronic Csf1r enhancer for their development.
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.
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Fluorescent Spectraviewer
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Our F4/80 (Cl:A3-1) Antibody has been referenced in >719 publications*
*Based on June 2020 data from CiteAb's antibody search engine.