F4/80 antibody | Cl:A3-1
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used for the identification of macrophages in injured soleus muscle by immunohistochemistry of formalin fixed, paraffin embedded tissue sections.
Image caption:
PCNA and F4/80 immunohistochemical expression after muscular injury. WT: wild type, KO: knockout. (A) Immunohistochemistry of PCNA. (Scale bar = 50μm), (B) Quantitative analysis of PCNA-positive cells, (C) Immunohistochemistry of F4/80. (Scale bar = 50μm), (D) Quantitative analysis of F4/80-positive cells. (B and D) Both PCNA and F4/80 expression in p21KO mice at 3 days after injury were the highest (p < 0.05).
From: Chinzei N, Hayashi S, Ueha T, Fujishiro T, Kanzaki N, Hashimoto S, et al. (2015)
P21 Deficiency Delays Regeneration of Skeletal Muscular Tissue.
PLoS ONE 10(5): e0125765.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the detection of infiltrating macrophages in tumor tissue by immunofluorescence.
Image caption:
Presence of inflammatory cells in tumor tissue. Macrophage and neutrophil infiltration are unaffected by loss of serglycin in RIP1-Tag2 tumors. Tumor sections from 15w RTposSGwt and RTposSGko mice were immunostained from the neutrophil and macrophage markers Gr-1 (a) and F4/80 (b) respectively. For Gr-1, the number of positive cells/mm2 was calculated (c) and there was no difference in infiltration of neutrophils between the two groups. For F4/80, the area of positive staining was measured (d) and although there was a slight trend to decreased macrophage infiltration in serglycin deficient animals, this was not significant. Each data point in represents an individual animal. Statistical analysis was performed using a two-tailed Mann-Whitney test. Error bars represent mean ± SEM.
From: Hamilton A, Basic V, Andersson S, Abrink M, Ringvall M (2015)
Loss of Serglycin Promotes Primary Tumor Growth and Vessel Functionality in the RIP1-Tag2 Mouse Model for Spontaneous Insulinoma Formation.
PLoS ONE 10(5): e0126688.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 (MCA497GA) antibody used for the detection of macrophage processes in mouse kidney tissue sections by immunofluorescence.
Image caption:
Dual immunofluorescence staining showing the association of F4/80+ cells with deposited C3. Shown is a representative immunofluorescence micrograph of a Crry−/−C3−/− kidney 7 days after transplantation into a wildtype recipient. Considerable deposition of C3 (green) is evident in the basolateral aspects of tubules, along with closely approximated F4/80+ cellular processes (red).
From: Chaves LD, Bao L, Wang Y, Chang A, Haas M, Quigg RJ (2014)
Loss of CD11b Exacerbates Murine Complement-Mediated Tubulointerstitial Nephritis.
PLoS ONE 9(3): e92051.Published customer image:
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the detection of infiltrating macrophages in tumor tissue by immunofluorescence.
Image caption:
Presence of inflammatory cells in tumor tissue. Macrophage and neutrophil infiltration are unaffected by loss of serglycin in RIP1-Tag2 tumors. Tumor sections from 15w RTposSGwt and RTposSGko mice were immunostained from the neutrophil and macrophage markers Gr-1 (a) and F4/80 (b) respectively. For Gr-1, the number of positive cells/mm2 was calculated (c) and there was no difference in infiltration of neutrophils between the two groups. For F4/80, the area of positive staining was measured (d) and although there was a slight trend to decreased macrophage infiltration in serglycin deficient animals, this was not significant. Each data point in represents an individual animal. Statistical analysis was performed using a two-tailed Mann-Whitney test. Error bars represent mean ± SEM.
From: Hamilton A, Basic V, Andersson S, Abrink M, Ringvall M (2015)
Loss of Serglycin Promotes Primary Tumor Growth and Vessel Functionality in the RIP1-Tag2 Mouse Model for Spontaneous Insulinoma Formation.
PLoS ONE 10(5): e0126688.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC-conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497F) used for the detection of F4/80 expressing cells in peritoneal cell exudates by flow cytometry.
Image caption:
Identification of resident peritoneal Mø subsets. PC from C57BL/6 were harvested and stained with fluorochrome-labeled antibodies directed against F4/80, CD19, CD11c and IAb for flow cytometry analysis. (A) Doublet cells were excluded according to forward scatter profiles (FSC-A and FSC-H). Subsequently, (B) CD19 high cells and (C) CD11c high cells were also excluded, and (D) F4/80+ cells were selected. (E) F4/80 and IAb expression defined three populations: LPM (F4/80highIAb-neg), SPM (F4/80lowIAb-high) and granulocytes (F4/80lowIAb-neg). These three subpopulations were purified by cell sorting on a FACS Vantage, and their morphology was evaluated ex vivo from cytospin slides (F), or after in vitro culture in chamber slides for 12 h (G). Slides were stained with hematoxylin and eosin (H&E) and analyzed by optical microscopy (40×).
From: Cassado AdA, de Albuquerque JAT, Sardinha LR, Buzzo CdL, Faustino L, Nascimento R, et al. (2011)
Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli.
PLoS ONE 6(7): e22141.
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FITC-conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497F) used for the detection of F4/80 expressing cells in peritoneal cell exudates by flow cytometry.
Image caption:
Zymosan and T. cruzi injection alters the Mø compartment of PerC. C57BL/6 mice were injected i.p. with zymosan (1 mg/mouse) or T. cruzi (106 parasites/mouse) and at 30 min (A) or 48 h (B) after stimulation, PC from naive and injected mice were harvested and stained as described in M&M. Sequential gates were made as shown in Fig. S1. Plots show the frequencies of each subpopulation. (C) F4/80lowMHCIIint cells present within PerC 48 h after injections were evaluated according the expression of Ly6C. Gray lines represent FMO [23], [25], the black lines show F4/80lowMHCIIint cells from zymosan- (hairline) or T. cruzi-(bold line)-exposed PerC. (D) Total numbers of SPM, LPM and monocytes 48 h after zymosan or T. cruzi exposure (within Mø gate) are shown in panel. Data are representative of more than 3 independent experiments.
From: Cassado AdA, de Albuquerque JAT, Sardinha LR, Buzzo CdL, Faustino L, Nascimento R, et al. (2011)
Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli.
PLoS ONE 6(7): e22141.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC-conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497F) used for the detection of F4/80 expressing cells in peritoneal cell exudates by flow cytometry.
Image caption:
SPM are more responsive than LPM and monocytes to infectious stimuli. C57BL/6 mice were injected i.p. with zymosan (1 mg/mouse) or T. cruzi (106 parasites/mouse) and PCs were harvested 30 min or 48 h after stimulation. (A) SPM and LPM from C57BL/6 zymosan-exposed mice were FACS-sorted 30 min after injection and the presence of internalized zymosan particles was observed by optical microscopy. Slides were made with 105 cells from purified Mø subsets, stained with H&E and analyzed by optical microscopy (40×). (B) Numbers represent the mean ±SD of internalized zymosan particles per cell in each Mø subset. *** p<0.001 when compared to the LPM group. (C) PC from control or 48 h-exposed mice were cultured for 6 h in the presence of brefeldin A with or without LPS (1 μg/ml) plus rIFN-γ (5 ng/ml). Titles above plots indicate in vivo - in vitro stimulations. Values inside gates represent the frequencies of IL-12-producing cells in each subpopulation. Data are representative of more than 3 independent experiments.
From: Cassado AdA, de Albuquerque JAT, Sardinha LR, Buzzo CdL, Faustino L, Nascimento R, et al. (2011)
Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli.
PLoS ONE 6(7): e22141.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used for the detection of macrophages in mouse lung and pancreas by immunohistochemistery on formalin fixed, paraffin tissue sections.
Image caption:
Changes in pancreatic and lung F4/80+ macrophage number during acute pancreatitis. Representative photomicrographs of pancreas (A) and lung (C) sections stained for F4/80 as indicator of macrophage infiltration (brown) 24 h after pancreatitis induction (original magnification, ×20). Quantification of F4/80+ cells showed a significantly increased enrichment in the pancreas from 9 h following pancreatitis compared to sham controls (B). No significant difference was observed in the number of lung F4/80+ cells between pancreatitis and sham control groups (D). Bars show mean ± SEM, n = 10 per group. **P<0.01, ***P<0.001, by two-tailed Student t-test.
From: Akbarshahi H, Menzel M, Posaric Bauden M, Rosendahl A, Andersson R (2012)
Enrichment of Murine CD68+CCR2+ and CD68+CD206+ Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.
PLoS ONE 7(10): e42654.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used for the evaluation of F4/80 expression on isolated Kuppfer and peritoneal cells by flow cytometry.
Image caption:
CLEC4F is co-expressed with F4/80 on liver Kupffer cells.
(A) CLEC4F and F4/80 immunohistochemistry of parafilm-embedded liver sections from wild-type and Clec4f−/− mice. (B) Double immunofluorescence of CLEC4F and F4/80 in wild-type livers was performed. Nuclei were counterstained with Hoechst 33342. Signals were determined by confocal microscope (magnification 10×63). (C) Coexpression of CLEC4F and F4/80 on Kupffer cells, but not peritoneal macrophages. Cells were double stained with Alexa Fluor 647-conjugated anti-F4/80 and PE-conjugated anti-CLEC4F mAb. Alexa Fluor 647-conjugated rat IgG2b and PE-conjugated mIgG1 were used as isotype controls.
From: Yang C-Y, Chen J-B, Tsai T-F, Tsai Y-C, Tsai C-Y, Liang P-H, et al. (2013)
CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver.
PLoS ONE 8(6): e65070.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the evaluation of F4/80 expression on thymic myeloid cells by flow cytometry.
Image caption:
Heterogeneous phenotype of thymic resident myeloid cells. (A) Thymic stromal cells were enriched by collagenase/dispase digestion followed by percoll density gradient centrifugation. Cells were then stained with anti-CD11b and anti-F4/80 and analyzed by FACS. Red circles mark the three different cell populations discussed in the text. In this experiment, populations #1, #2 and #3 represented 0.02%, 0.43% and 0.61% of the analyzed cells, respectively. While the absolute cell number varied as a result of the preparation, the percentage of each cell type relative to each other was consistent over many experiments. (B) Size and surface marker expression (blue lines) of each population was determined by FACS. Populations were identified as in (A) and stained with the antibodies as indicated. Isotype controls (red lines) were used to correct differences in the autofluorescence of each cell population. (C) Individual cells from populations #2 and #3 were sorted onto glass slides followed by staining with H&E. Data are representative of more than ten experiments (a and b) and three experiments (c).
From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010)
Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used for the evaluation of F4/80 expression on thymic myeloid cells following irradiation by immunofluorescence.
Image caption:
Time course analysis of F4/80 positive cells following irradiation.
Thymuses from wild type B6 mice were harvested and frozen post-irradiation at the indicated times (1 hour through 24 hours). Sections were stained with anti-F4/80 (red), which identifies macrophages and eosinophils. Panel labeled "control" was a section stained with secondary antibody only. Panel labeled “w/o irr.” was a section from a nonirradiated mouse. Original magnification was 200X.
From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010)
Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the evaluation of F4/80 expression on thymic myeloid cells following irradiation by flow cytometry.
Image caption:
Influx of innate cells rapidly declines twenty-four hours after irradiation. Thymuses were harvested from mice at the indicated times after irradiation. Stromal cells were enriched as described and stained with anti-CD11b, -F4/80, SiglecF and -Ly-6G. Numbers in the plots are the percentage of cells within the electronic gate. N = 6 and the data show the results from two independent experiments. The mean is shown by a horizontal bar and statistics were calculated with 2-tailed, nonpaired Mann-Whitney test. * = <0.01; ** = <0.001; *** = <0.0001..
From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010)
Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the evaluation of F4/80 expression on spleen and bone marrow cells by flow cytometry.
Image caption:
FACS analyses of the innate cells in the spleen and bone marrow from WT and Csf op/op mice. Cells from the spleen and bone marrow from WT and Csfop/op mice were stained for CD11b and F4/80. Eosinophils and neutrophils were identified by staining cells for CD11b and SiglecF or CD11b and Gr-1, respectively. Percent of cells in each area is indicated in the Figure.
From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010)
Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the identification of macrophages in mouse spleen using immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Depletion of F4/80-expressing cells in spleens derived from clodrolip-treated Ptchflox/floxERT2+/− mice. Immunohistochemical analysis using an anti-F4/80 antibody of paraffin-embedded spleens of Ptchflox/floxERT2+/− mice treated with empty liposomes or clodrolip.
From: König S, Nitzki F, Uhmann A, Dittmann K, Theiss-Suennemann J, Herrmann M, et al. (2014)
Depletion of Cutaneous Macrophages and Dendritic Cells Promotes Growth of Basal Cell Carcinoma in Mice.
PLoS ONE 9(4): e93555.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used for the identification of infiltrating macrophages in tumor tissue by immunofluorescence.
Image caption:
CSF1 has no effect on tumor growth but increases percent tumor TEMs and augments angiogenesis. (A) After two weeks of treatment, tumors were removed, homogenized and immunostained with antibodies specific for F4/80 and Tie2 to identify total F4/80+ cells and F4/80+/Tie2+ cells (Tie2-expressing macrophages, TEMs). While there was a marked increase in total F4/80+ macrophages with CSF1 treatment, the percent of F4/80+/Tie2+ TEMs was significantly increased in response to CSF1 suggesting a regulatory role for CSF1 in expanding the TEM population. N = 5 mice per group and results represent the mean ± SEM of total F4/80+ and F4/80+/Tie2+ TEMs within the tumors. (B, top and bottom left) PyMT tumors without CSF1 treatment and (top and bottom right) with CSF1 treatment immunostained with CD31 for blood vessels, F4/80 for macrophages, Tie2 for F4/80+/Tie2+ TEMS, and DAPI. Confocal images (using 60× objective (top) and with 3× zoom (bottom) suggest an increase in both F4/80 macrophages and F4/80+/Tie2+ TEMS in the CSF1-treated tumors. Multiply overlap indicates those areas where F4/80 and Tie2 positivity overlap. Individual stains are in Supplementary Figure 3. (C, top) Orthotopically implanted PyMT mammary tumors in wild type C57Bl/6 female mice were allowed to become palpable then intraperitoneally treated with PBS (PBS), CSF1 (100 ng in 100 μls) (CSF1), a neutralizing antibody for the CSF1R (50 mg/kg) 4 hours prior to CSF1 treatment (100 ng in 100 μls) (CSF1R NAb+CSF1), the CSF1R antibody alone (CSF1R NAb), an isotype antibody (50 mg/kg) 4 hours prior to CSF1 (100 ng in 100 μls) treatment (CSF1+IgG), or the isotype antibody alone (IgG) three times per week for two additional weeks. The tumors were immunostained with a CD31-Alexa Flour 546 antibody to recognize endothelial cells that comprise blood vessels. Qualitatively, CSF1 treatment increased the percent of CD31-postitive pixels per high powered field compared to PBS treated tumors, while the neutralizing antibody to CSF1R suppressed the CSF1 effect on angiogenesis. (B, bottom) Quantitatively, the percent of CD31+ pixels per high powered field were quantified as blood vessels (angiogenesis) using Adobe Photoshop histogram analysis. CSF1 treatment significantly increased CD31-positive pixels (angiogenesis) compared to PBS. The neutralizing antibody for CSF1R significantly reduced the ability of CSF1 to up-regulate angiogenesis. N = 5 mice per group and results represent the mean ± SEM of percent CD31-positive pixels per high powered field (HPF).
From: Forget MA, Voorhees JL, Cole SL, Dakhlallah D, Patterson IL, Gross AC, et al. (2014)
Macrophage Colony-Stimulating Factor Augments Tie2-Expressing Monocyte Differentiation, Angiogenic Function, and Recruitment in a Mouse Model of Breast Cancer.
PLoS ONE 9(6): e98623.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used for the identification of infiltrating macrophages in the spleen of Entamoeba histolytica infected mice by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Histological and immunohistochemical characterization of cell infiltrates during ALA. (A) H&E staining of mouse liver abscesses (indicated by the square in the top row of images) at the indicated times post-infection with E. histolytica trophozoites. (B) PAS staining shows E. histolytica trophozoites (arrowheads) within the abscess. (C and D) Tissue sections were stained with anti-7/4 (C) and anti-F4/80 (D) antibodies followed by HRP-conjugated secondary antibody to detect neutrophils and macrophages, respectively (brown).
From: Helk E, Bernin H, Ernst T, Ittrich H, Jacobs T, Heeren J, et al. (2013)
TNFα-Mediated Liver Destruction by Kupffer Cells and Ly6Chi Monocytes during Entamoeba histolytica Infection.
PLoS Pathog 9(1): e1003096.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for immunohistochemistry on mouse brain sections
Image caption:
Representative photographs of cortical area in (a) CD40 def. and (b) wild-type mice stained with F4/80 antibody at 8 to 10 weeks old (each bar represents 0.1 mm).
From: Laporte et al.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used for the detection of microglia in mouse brain by immunofluorescence
Image caption:
Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real–time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean ± SEM; *P<0.05, **P<0.01, and ***P<0.001 versus sham group, #P<0.05, ##P<0.01, and ###P<0.001 versus vehicle group (n = 6–9 mice/group for BDNF protein and 7–9 mice/group for phospho-CREB, one-way ANOVA; n = 6–8 mice/group for BDNF mRNA, one-way ANOVA or Student's t-test). The scale bar is 50 μm. Ipsi: ipsilateral cortex; Contra: contralateral cortex.
From: Wu C-H, Hung T-H, Chen C-C, Ke C-H, Lee C-Y, et al. (2014)
Post-Injury Treatment with 7,8-Dihydroxyflavone, a TrkB Receptor Agonist, Protects against Experimental Traumatic Brain Injury via PI3K/Akt Signaling.
PLoS ONE 9(11): e113397.
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Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the detection of infiltrating macrophages in mouse kidney by immunohistochemistry on paraffin embedded material
Image caption:
Reduction in cellular infiltration and inflammatory markers in UUO kidneys exposed to telmisartan or PXS64. Untreated UUO kidneys showed increased F4/80, CD68 and CD45 positively stained cells as compared to the sham operated control animals. PXS64 significantly reduced F4/80 and CD45 positive stained cells (Fig. 6C and E) with a trend to a reduction in CD68 cells, although this was not statistically significant (Fig. 6D). Telmisartan treated kidneys showed a reduction in F4/80 positive cells but no difference in CD45 or CD68 stained cells, suggesting a differential action of PXS64 and telmisartan in modifying cellular infiltration. Results are presented as mean showed (n = 8, *P<0.05 vs. UUO, ** P<0.01 vs. UUO). Magnification x 400.
From: Zhang J, Wong MG, Wong M, Gross S, Chen J, et al. (2015)
A Cationic-Independent Mannose 6-Phosphate Receptor Inhibitor (PXS64) Ameliorates Kidney Fibrosis by Inhibiting Activation of Transforming Growth Factor-β1.
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Rat anti Mouse F4/80 antibody, clone Cl:A3-1 (MCA497G) used for the identification of macrophages in a murine colitis model by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Nur77-/- mice show more macrophage and T-cell influx in TNBS-induced colitis. The numbers of T-cells in the mucosa were counted and the influx was calculated by taking the ratio of Healthy / TNBS. Representative photomicrographs of sections stained for T-cells (anti-CD3) are shown (A). mRNA was extracted from colon and qPCR was performed for FoxP3 (B). cDNA levels were normalized for 36B4 housekeeping gene expression. Macrophage surface was quantified in the mucosa and the number of macrophages was counted in the muscle. The ratio of Healthy / TNBS was calculated. Representative photomicrographs of sections stained for macrophages (anti-F4/80) are shown (C). The numbers of Ki67 positive cells were counted per crypt and representative photomicrographs are shown (D). Colon lysates were prepared to determine IL-6, TNFα, and MCP-1 levels in the colon (E). A and C Original magnification x100, D x200. Each symbol represents 1 animal. Data are presented as mean ±SEM; *p<0.05, **p<0.01, ***p<0.001. Scale bars represent 1mm. nr = number, AU = Arbitrary units, FoxP3 = Forkhead box P3 (marker for regulatory T-cells).
From: Hamers AAJ, van Dam L, Teixeira Duarte JM, Vos M, Marinković G, van Tiel CM, et al. (2015)
Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NFκB Activity in Macrophages.
PLoS ONE 10(8): e0133598.
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Rat anti MouseF4/80 antibody, clone A3-1 (MCA497R) used for the identification of macrophages in murine kidney by immunofluorescence and immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Clodronate liposome mediated macrophage depletion in Col4a3KO mice. (A) KO male mice were dosed intraperitonally with CL (KO+CL) or PBSL (KO+PBSL) as control. Animals were entered in the study at the age of 4 weeks and were continually dosed until the age of 8 weeks. Injections were repeated every second day, except for the first two doses, which were injected on consecutive days. (B-D) CL significantly reduced F4/80 positive macrophage infiltrates in KO+CL kidney as compared to KO+PBS or KO mice at the age of 8 weeks. (B, C) Representative images and quantitative assessment of F4/80 stained macrophages in kidney sections from KO+CL and KO+PBSL reveal marked reduction in macrophage infiltrates following CL dosing. Scale bar: 100 μm. (D) Real-time PCR analysis of F4/80 mRNA expression in KO+CL, KO+PBSL, KO, and WT littermates at the age of 8 weeks. Significant reduction in F4/80 mRNA expression following CL dosing was observed. PBSL did not affect endogenous F4/80 mRNA expression as shown by similar F4/80 mRNA expression in KO and KO+PBSL mice. (E) Real-time PCR analysis of CD204 and CD206 mRNA showed significant reduction in the expression of both genes in KO kidney upon CL treatment. (F) Representative images and quantitative assessment of F4/80 and CD206 stained kidney sections from KO+CL and KO+PBSL revealed marked reduction in M1 (F4/80+CD206-) and M2 (F4/80+CD206+) macrophages. Asterisk: F4/80+CD206- M1 macrophages, arrow: F4/80+CD206+ M2 macrophages. Scale bar: 20 μm. Welch corrected ANOVA followed by posthoc pairwise t-test with a Bonferroni correction (C,D,E) and unpaired t-test (F) was used to evaluate differences between groups. (C,D,E,F) n = 5 mice per group. CL: clodronate liposomes; PBSL: PBS liposomes.
From: Kim M, Piaia A, Shenoy N, Kagan D, Gapp B, Kueng B, et al. (2015)
Progression of Alport Kidney Disease in Col4a3 Knock Out Mice Is Independent of Sex or Macrophage Depletion by Clodronate Treatment.
PLoS ONE 10(11): e0141231.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the identification of murine macrophages in the kidney by immunohistochemistry on formalin fixed cryosections.
Image caption:
Immunostainings of macrophages and neutrophils in the kidneys of M-Rac1 FC and KO mice. (A) Representative micrographs of F4/80 immunostaining in the kidneys of Vehicle- or LPS-injected M-Rac1 FC and KO mice. Original magnification x 200. (B) Quantitative analysis of F4/80-positive macrophages. Data are means ± s.e.m. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 5 per each group. (C) Representative micrographs of Ly-6B.2 (mAb 7/4) immunostaining. (D) Quantitative analysis of Ly-6B.2-positive neutrophils. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 6 per group.
From: Citation: Nagase M, Kurihara H, Aiba A, Young MJ, Sakai T (2016)
Deletion of Rac1GTPase in the Myeloid Lineage Protects against Inflammation-Mediated Kidney Injury in Mice.
PLoS ONE 11(3): e0150886.
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Alexa Fluor® 647 conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497A647) used for the detection of murine Kupffer cells by immunofluorescence
Image caption:
Hepatic stellate cells are the major source of CXCL1, as shown by both quantification of secretion and in situ localization. (A) Quantification of CXCL1 secretion in enriched fractions of hepatocytes, KCs, LSECs and HSCs, freshly isolated and stimulated in vitro with LPS (1 ng/mL LPS, black squares) during 24 hours. Data are representative of three separate experiments with six mice in each group; #P<.05. (B) In-situ localization of CXCL1 in the liver. Immunofluorescent detection for CXCL1 (red) and liver cells nuclei (blue) for nuclei first shows CXCL1 expression in the sinusoids throughout liver parenchyma. (C) Higher resolution shows that CXCL1 (red) is expressed by sub-endothelial cells, which also store retinol droplets in separate compartments, as shown by CRBP1 staining (green). The Cellular Retinol Binding Protein-1 (CRBP-1) is the best marker to detect simultaneously both resting (Glial Fibrillary Acidic Protein, GFAP+) and activated (α-Smooth Muscle Actin, αSMA+) stellate cells in situ. Alexa Fluor-546-CXCL1 (red) staining does not colocalize either with Tie2-GFP in LSECs (green, upper panel), or F4/80 in KCs (blue, middle panel), but with AlexaFluor-488-CRBP1 (green, lower panel), staining both resting and activated HSCs. TOPRO3 was used for nuclei vizualisation.
From: Bigorgne AE, John B, Ebrahimkhani MR, Shimizu-Albergine M, Campbell JS, Crispe IN (2016)
TLR4-Dependent Secretion by Hepatic Stellate Cells of the Neutrophil-Chemoattractant CXCL1 Mediates Liver Response to Gut Microbiota.
PLoS ONE 11(3): e0151063.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used for the demonstration of microglia in mouse brain by immunohistochemistry on formalin fixed 40μ vibratome sections.
Image caption:
CN-105 reduces brain microglial activation after ICH.
(A) Representative images of F4/80-labelled microglia in ipsilateral hippocampi at 5 days after ICH in mice treated with vehicle or CN-105.
From: Lei B, James ML, Liu J, Zhou G, Venkatraman TN, Lascola CD, Acheson SK, Dubois LG, Laskowitz DT, Wang H.
Neuroprotective pentapeptide CN-105 improves functional and histological outcomes in a murine model of intracerebral hemorrhage.
Sci Rep. 2016 Oct 7;6:34834.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used for the identifcation of macrophages in kidney sections by immunohistochemistry on cryosections.
Image caption:
Renal markers of inflammation at Week 32.
F4/80 representative images at 200x magnification, E. TGF-β mRNA expression, F. MCP-1 mRNA expression. Results are expressed as mean ± SEM, N = 4–6. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 compared to CC.
From: Glastras SJ, Chen H, Tsang M, Teh R, McGrath RT, Zaky A, et al. (2017)
The renal consequences of maternal obesity in offspring are overwhelmed by postnatal high fat diet.
PLoS ONE 12(2): e0172644.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497G) used for the identification of macrophages in kidneys and livers of mice by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Focal expression of Irga6 in healthy mouse liver and kidney.
T cells and macrophages/dendritic cells are present in the mononuclear cores of the liver and kidney patches. Wax sections of the livers and kidneys from adult C57BL/6 mice were stained for Irga6 (brown/red) and CD3 or F4/80 (blue). For Irga6 and CD3 double staining in liver, consecutive serial sections were analysed (numbered 1, 2, 3, 4). Arrows point to CD3 and F4/80 positive cells. The microscope magnification is 200×; frames show enlargements.
From: Zeng J, Howard JC.
Spontaneous focal activation of invariant natural killer T (iNKT) cells in mouse liver and kidney.
BMC Biol. 2010 Nov 30;8:142.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F480, clone A3-1 (MCA497R) used for the demonstration of Kupffer cells in mouse liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Expression of F4/80 and CD68 in liver tissue assessed by immunostaining using anti-F4/80 or anti-CD68 antibody.
From: Citation: Ibusuki R, Uto H, Oda K, Ohshige A, Tabu K, Mawatari S, et al. (2017)
Human neutrophil peptide-1 promotes alcohol-induced hepatic fibrosis and hepatocyte apoptosis.
PLoS ONE 12(4): e0174913.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used to identify macrophages in the murine vastus lateralis by immunofluorescence on cryostat sections.
Image caption:
Combination of bone fracture and alendronate treatment causes loss of myogenic cells.
PBS (Control group) or alendronate (Alendronate group) was subcutaneously injected three times at seven-day intervals. The femur was broken and vastus lateralis muscle injured by cold shock on day 14. Then the vastus lateralis muscle was removed on day 1, 4, or 7 after the bone fracture and cold injury. Sections were subjected to immunostaining with antibodies to M-cadherin (green in A-F), F4/80 (green in G-L), CD31 (green in M-R), and laminin α2 (red in A-R). Nuclei were stained with DAPI (blue in A-R). Arrows represent M-cadherin-positive muscle satellite cells in (C). Scale bars, 40 μm in (A-F), 20 μm in (G-R). Images represent a part of each original picture. Numbers represent numbers of M-cadherin-positive muscle satellite cells/myogenic progenitor cells in each original picture (0.17 mm2) in (A-F). Percentages of F4/80-positive cell-occupied area of each original picture (0.68 mm2) are shown in (G-L). Numbers of CD31-positive capillaries per mm2 of each original picture are shown in (O and R).
From: Kawada S, Harada A, Hashimoto N (2017)
Impairment of cold injury-induced muscle regeneration in mice receiving a combination of bone fracture and alendronate treatment.
PLoS ONE 12(7): e0181457.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497GA) used to identify infiltrating macrophages in mice by immunohistochemistry on formalin fixed, paraffin embedded tissue sections
Image caption:
Middle ear histology indicates chronic otitis media in Nischedsn/edsn mice.
(J) Immunohistochemical staining of a Nischedsn/edsn ear with OM using an F4/80 antibody shows a cellular exudate rich in macrophages (brown). Representative image from four mice scale bar =100μm.
From: Crompton M, Purnell T, Tyrer HE, Parker A, Ball G, Hardisty-Hughes RE, et al. (2017)
A mutation in Nischarin causes otitis media via LIMK1 and NF-κB pathways.
PLoS Genet 13(8): e1006969.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the identification of macrophages in mouse spleen sections by immunofluorescence.
Image caption:
Localization of IgG2c+ (red) plasma cells in the spleens of 4 month old w.t. and MFG-E8-/- mice was examined by immunofluorescence staining. Red pulp macrophages and Marginal zone macrophages were visualized by anti-F4/80(green, upper) and anti-CD169 (green, lower), respectively. The images are representative of at least 5 mice in each strain.
From: Peng Y (2018)
B cell responses to apoptotic cells in MFG-E8-/- mice.
PLoS ONE 13(10): e0205172.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Moouse F4/80 antibody, clone A3-1 (MCA497G) used to identify macrophages in mouse liver by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
F4/80 staining. F4/80 activities in livers are examined at 1 and 2 days with immunostaining. (A) 1 day; (B)2 days. For all panels, representative images are shown and arrows denote positive staining. ns p ≥0.05, * p <0.05.
From: Wang H, Zhang H, Wang Y, Yang L, Wang D.
Embelin can protect mice from thioacetamide-induced acute liver injury.
Biomed Pharmacother. 2019 Oct;118:109360.
doi: 10.1016/j.biopha.2019.109360.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used to evaluate F4/80 expression in murine kidney by western blotting and immunofluorescence.
Image caption:
UMOD expression amplifies UUO‐associated interstitial inflammation. Interstitial inflammation is a hallmark feature of kidney fibrosis. Interstitial macrophage density, quantified by kidney F4/80 protein western blotting (n = 5 for sham groups; n = 7 for UUO groups), was significantly lower in the UMOD−/− groups by 41–58% at all time‐points (A, B). Representative day 21 immunofluorescence photomicrographs illustrate a smaller interstitial area expressing the F4/80 macrophage protein (green) in the UMOD−/− kidney (C). Kidney MCP‐1 mRNA levels were significantly lower in the UMOD−/− mice on UUO days 7, 14, and 21 (n = 3 for sham groups; n = 7 for UUO groups) (D). Bar graphs represent means +1SEM; * indicates P value <0.05. Photomicrograph magnification: ×40. Scale bar: 25 μm.
From: Maydan O, McDade PG, Liu Y, Wu XR, Matsell DG, Eddy AA.
Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy.
Physiol Rep. 2018 Mar;6(6):e13654.
doi:10.14814/phy2.13654.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the evaluation of F4/80 expression on thymic cells by flow cytometry.
Image caption:
Impaired clearance of apoptotic thymocytes in Csf1op/op mice. (A) Thymic stromal cell subpopulations from WT (Csf1op/+) and osteopetrosis (Csf1op/op) mice were stained with the indicated antibodies and analyzed by FACS. Numbers indicate the percentage of cells within the circles. The red circle marks population #1, which is not present in Csf1op/op mice. (B) Comparison of thymic stromal cell subpopulations between WT (Csf1op/+) and osteopetrosis (Csf1op/op) mice 16 hours after irradiation. Thymic stromal cells were enriched and stained for CD11b and F4/80. (C) Representative images showing the frequency of apoptotic cells in WT (Csf1op/+) and Csf1op/op mice in nonirradiated mice in mice 24 hrs receiving 1Gy irradiation. Apoptotic cells were detected by TUNEL (green) and their cortical localization was visualized by counterstaining of thymic sections with an antibody against DEC205 (red). Original magnification was 100X. Sections are representative of multiple samples from more than ten experiments.
From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010)
Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the identification of macrophages in formalin fixed, paraffin embedded murine skin tissue sections by immunohistochemistry.
Image caption:
L-tryptophan administration reverses stress-induced alterations on inflammatory response and lipid peroxidation. Mice were daily submitted to restraint stress and treated with L-tryptophan (Trp) or vehicle until euthanasia. Two days after the beginning of the stress protocol, two full-thickness excisional lesions (8-mm diameter) were made on the dorsal skin. Five days after wounding, lesions were collected and the number of myeloperoxidase (MPO)-positive neutrophils (B), F4/80-positive macrophages (C) and CD-3 positive T lymphocytes (D) were counted. Representative images (A) of staining for neutrophils, macrophages and T cells on wound area were presented. In addition, the protein levels of monocyte chemotactic protein-1 (MCP-1) (12 kDa) (E) were estimated in wound lysate by western-blotting. Representative images for immunoblotting for MCP-1 (F) were presented. The β-actin (42 kDa) was used as a loading control protein. Vertical black lines show non-adjacent bands from the same blot. To evaluate the oxidative damage in lipids, the levels of lipid peroxides (G) were measured in wound lysate using colorimetric assay. Data (n = 5) are presented as mean ± SEM. #p<0.05 vs. stressed (ST) group. Bar = 50 μm.
From: Bandeira LG, Bortolot BS, Cecatto MJ, Monte-Alto-Costa A, Romana-Souza B (2015)
Exogenous Tryptophan Promotes Cutaneous Wound Healing of Chronically Stressed Mice through Inhibition of TNF-α and IDO Activation.
PLoS ONE 10(6): e0128439.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone 3A-1 (MCA497R) used to identify macrophages in mouse muscle graft sections by immunofluorescence.
Image caption:
Autografted and allografted muscles: morphology and osteopontin expression. Cryosections of EDL muscle grafts at 5 (a, b) or 3 (c, d) days post-surgery. a H&E-stained sections. Arrows indicate regenerating fibres (small fibres with centrally located nuclei). b Sections stained with anti-MyHCemb (green), anti-laminin (red) and DAPI, as used for quantitation of regenerating fibres. c Sections stained with anti-osteopontin (red), anti-desmin (myoblasts; green) and DAPI. In wild-type (WT) EDL muscle grafted into a wild-type host, anti-osteopontin stained both myoblasts (yellow) and non-myoblast mononuclear cells (red). In osteopontin-null (KO) muscle grafted into an osteopontin-null host, no osteopontin was detected. In allografted osteopontin-null muscle in a wild-type host, osteopontin was only detected in non-myoblast mononuclear cells. In allografted wild-type muscle in an osteopontin-null host, osteopontin was only detected in myoblasts. Arrows and arrowheads indicate myoblasts and non-myoblast mononuclear cells, respectively. d Sections stained with anti-osteopontin (red), anti-F4/80 (macrophages; green) and DAPI. In autografted wild-type muscle, anti-osteopontin stained macrophages (*; yellow) and some additional structures (arrows; red). In autografted osteopontin-null muscle, no osteopontin was detected. In allografted osteopontin-null muscle in a wild-type host, osteopontin was only observed in macrophages (*; yellow). In allografted wild-type muscle in an osteopontin-null host, weak osteopontin staining was observed in some non-macrophage structures (arrows), but not in macrophages (†; green). Bars = 50μm
From: Wasgewatte Wijesinghe DK, Mackie EJ, Pagel CN.
Normal inflammation and regeneration of muscle following injury require osteopontin from both muscle and non-muscle cells. Skelet Muscle. 2019; 9(1):6.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497R) used for the identification of macrophages in tumor tissues by immunohistochemistry on formalin fixed cryosections. Comparison is with the distribution of CD11c+ dendritic cells (MCA1369GA) in similar tissue sections.
Image caption:
IHC analysis of Colon-26 tumors untreated and INT230-6-treated tumors ~10 days post dose. Representative images (40× magnification) of IHC staining for F4/80 (macrophages) and CD11c (dendritic cells). In general, the staining appeared more evident in the INT230-6-treated tumors, particularly toward the more necrotic areas.
From: Bender LH, Abbate F, Walters IB.
Intratumoral Administration of a Novel Cytotoxic Formulation with Strong Tissue Dispersive Properties Regresses Tumor Growth and Elicits Systemic Adaptive Immunity in In Vivo Models.
Int J Mol Sci. 2020;2 (12):E4493.
doi:10.3390/ijms21124493
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Pacific Blue® conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497PB) used for the dendritic cell gating process from mouse white adipose tissue by flow cytometry.
Image caption:
A representative gating scheme of dendritic cell and macrophage populations in the epididymal white adipose tissues. For analysis of dendritic cell and macrophage populations, stromal vascular cells obtained from epididymal white adipose tissues were stained with FITC–conjugated anti-CD206, PE–conjugated anti-CD11c, PerCP–conjugated anti-CD45, PE-Cy7–conjugated anti-F4/80*, APC–conjugated anti-CD64 and APC-Cy7–conjugated anti-MHC class II antibodies.
From: Choi EW, Lee M, Song JW, Kim K, Lee J, Yang J, Lee SH, Kim IY, Choi JH, Seong JK.
Fas mutation reduces obesity by increasing IL-4 and IL-10 expression and promoting white adipose tissue browning.
Sci Rep. 2020 Jul 20;10(1):12001.
doi: 10.1038/s41598-020-68971-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License. * should read "Pacific Blue® conjugated F4/80.
Rat anti Mouse F4/80:Alexa Fluor® 488
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Alexa Fluor® 647
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:APC
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Biotin
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:FITC
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Low Endotoxin
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Pacific Blue®
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:RPE
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:RPE-Alexa Fluor® 647
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:RPE-Alexa Fluor® 750
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Alexa Fluor® 700
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Starbright Blue 700
Rat anti Mouse F4/80:Amethyst Orange
F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
Rat anti Mouse F4/80:Starbright Violet 515
- Product Type
- Monoclonal Antibody
- Clone
- Cl:A3-1
- Isotype
- IgG2b
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA497A488T | F | 0.25 ml |
|
|
| MCA497A488 | F | 100 Tests/1ml |
|
|
| MCA497A647T | F | 25 Tests/0.25ml |
|
|
| MCA497A647 | F | 100 Tests/1ml |
|
|
| MCA497APCT | F | 25 Tests/0.25ml |
|
|
| MCA497APC | F | 100 Tests |
|
|
| MCA497BT | F | 25 µg |
|
|
| MCA497B | F | 0.1 mg |
|
|
| MCA497BB | F | 0.5 mg |
|
|
| MCA497FT | F IF | 25 µg |
|
|
| MCA497FA | F IF | 50 µg |
|
|
| MCA497F | F IF | 0.1 mg |
|
|
| MCA497FB | F IF | 0.5 mg |
|
|
| MCA497EL | C F FN IP P* R RE WB | 0.5 mg |
|
|
| MCA497PBT | F | 25 Tests/0.25ml |
|
|
| MCA497PB | F | 100 Tests/1ml |
|
|
| MCA497RT | C EM F IF IP P* R RE WB | 25 µg |
|
|
| MCA497GA | C EM F IF IP P* R RE WB | 0.1 mg |
|
|
| MCA497R | C EM F IF IP P* R RE WB | 0.25 mg |
|
|
| MCA497G | C EM F IF IP P* R RE WB | 0.5 mg |
|
|
| MCA497PET | F IF | 25 Tests |
|
|
| MCA497PE | F | 100 Tests |
|
|
| MCA497P647 | F | 100 Tests/1ml |
|
|
| MCA497P750 | F | 100 Tests/1ml |
|
|
| MCA497 | C EM F IF IP P* R RE | 2 ml |
|
|
| MCA497A700T | F | 25 Tests/0.25ml |
|
|
| MCA497A700 | F | 100 Tests/1ml |
|
|
| MCA497SBB700 | F | 100 Tests/0.5ml |
|
|
| MCA497AMO | F | 0.1 mg |
|
|
| MCA497SBV515 | F | 100 Tests/0.5ml |
|
Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognizes the murine F4/80 antigen, a ~160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).
Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al. 1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al. 1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin, et al. 2005)
Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999).
A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.
Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al. 1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al. 1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin, et al. 2005)
Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999).
A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE)-Alexa Fluor® 647 - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE)-Alexa Fluor® 750 - lyophilized
- Product Form
- Tissue Culture Supernatant - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Product Form
- Purified IgG conjugated to StarBright Blue 700 - liquid
- Product Form
- Purified IgG conjugated to Amethyst Orange - liquid
- Product Form
- Purified IgG conjugated to StarBright Violet 515 - liquid
- Reconstitution
- Pack Size: 25 Tests/0.25mlReconstitute with 0.25ml distilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water
- Reconstitution
- Pack Size: 25 TestsReconstitute with 0.25 ml distilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- None present
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350 - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350 - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- Pack Size: 25 µg, 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- Pack Size: 25 µg, 50 µg, 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 µg, 0.1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 µg, 50 µg, 0.1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 TestsPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q61549
- Entrez Gene
- Emr1
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0004930 G-protein coupled receptor activity
- GO:0007218 neuropeptide signaling pathway
- GO:0009897 external side of plasma membrane
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Regulatory
- For research purposes only
Applications of F4/80 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat Pack Size: 25 µg, 0.1 mg 1/10 Pack Size: 0.5 mg |
1/10 Pack Size: 25 µg, 0.1 mg 1/50 Pack Size: 0.5 mg |
|
| Flow Cytometry | Neat Pack Size: 50 µg 1/10 Pack Size: 0.5 mg |
Neat Pack Size: 25 µg, 0.1 mg 1/10 Pack Size: 50 µg 1/50 Pack Size: 0.5 mg |
|
| Immunofluorescence | |||
| Flow Cytometry | 1/100 | 1/200 | |
| Functional Assays | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunohistology - Resin | |||
| Immunoprecipitation | |||
| Radioimmunoassays | |||
| Western Blotting | |||
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immuno-electron Microscopy | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunohistology - Resin | |||
| Immunoprecipitation | |||
| Radioimmunoassays | |||
| Western Blotting | |||
| Flow Cytometry | Neat | ||
| Immunofluorescence | |||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/10 | |
| Immuno-electron Microscopy | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunohistology - Resin | |||
| Immunoprecipitation | |||
| Radioimmunoassays | |||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat |
- 1 Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.
- 1 Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.
- 1 Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Streptavidin:RPE | STAR4A | F | 1 ml |
|
|
| Streptavidin:APC | STAR119 | F | 100 Tests |
|
|
| Goat anti Rat IgG (H/L):HRP | 305005 | C E IB | 1 ml |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
|
| Goat anti Rat IgG2b:HRP | STAR114P | C E P WB | 0.5 mg |
|
|
| Goat anti Rat IgG (H/L):HRP | 305005 | C E IB | 1 ml |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
|
| Goat anti Rat IgG2b:HRP | STAR114P | C E P WB | 0.5 mg |
|
|
| Goat anti Rat IgG (H/L):HRP | 305005 | C E IB | 1 ml |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
|
| Goat anti Rat IgG2b:HRP | STAR114P | C E P WB | 0.5 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2b Negative Control:RPE-Alexa Fluor® 750 | MCA6006P750 | F | 100 Tests/1ml |
|
Useful Reagents Available
Application Based External Images
Flow Cytometry
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Product Specific References
Source Reference
-
Austyn, J.M. & Gordon, S. (1981) F4/80, a monoclonal antibody directed specifically against the mouse macrophage.
Eur J Immunol. 11 (10): 805-15.
Antibody Characterization Reference
-
Hume, D.A. et al. (1984) The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia.
Anat Rec. 210 (3): 503-12. -
Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.
J Exp Med. 161 (3): 475-89.
References for F4/80 antibody
-
Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues.
Curr Top Microbiol Immunol. 181: 1-37. -
Warschkau, H. & Kiderlen, A.F. (1999) A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes.
J Immunol. 163 (6): 3409-16. -
Lin, H.H.et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.
J Exp Med. 201 (10): 1615-25. -
Chan, R.J. et al. (2005) Human somatic PTPN11 mutations induce hematopoietic cell hypersensitivity to granulocyte-macrophage colony stimulating factor
Blood. 105: 3737-3742. -
Moore, K.J. et al. (2000) Divergent response to LPS and bacteria in CD14-deficient murine macrophages.
J Immunol. 165 (8): 4272-80. -
Dandekar, A.A.et al. (2004) Bystander CD8 T-cell-mediated demyelination is interferon-gamma-dependent in a coronavirus model of multiple sclerosis.
Am J Pathol. 164: 363-9. -
Muto, A. et al. (2011) Eph-B4 prevents venous adaptive remodeling in the adult arterial environment.
J Exp Med. 208 (3): 561-75. -
Pizza, F.X. et al. (2005) Neutrophils contribute to muscle injury and impair its resolution after lengthening contractions in mice.
J Physiol. 562 (Pt 3): 899-913. -
Tarallo, V. et al. (2011) The biflavonoid amentoflavone inhibits neovascularization preventing the activity of proangiogenic vascular endothelial growth factors.
J Biol Chem. 286: 19641-51. -
Rivollier, A. et al. (2012) Inflammation switches the differentiation program of Ly6Chi monocytes from antiinflammatory macrophages to inflammatory dendritic cells in the colon.
J Exp Med. 209: 139-55. -
Hemmi, H. et al. (2009) A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.
J Immunol. 182:1278-86. -
Seitz, O. et al. (2010) Wound Healing in Mice with High-Fat Diet- or ob Gene-Induced Diabetes-Obesity Syndromes: A Comparative Study
Exp Diabetes Res. 2010: 476969. -
Miao, E.A. et al. (2011) Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria.
Nat Immunol. 11: 1136-42. -
Wang, X. et al. (2011) Activation of the cholinergic antiinflammatory pathway ameliorates obesity-induced inflammation and insulin resistance
Endocrinology. 152: 836-46. -
Cunningham, O. et al. (2009) Microglia and the urokinase plasminogen activator receptor/uPA system in innate brain inflammation.
Glia. 57: 1802-14. -
Gornicka, A. et al. (2012) Adipocyte hypertrophy is associated with lysosomal permeability both in vivo and in vitro: role in adipose tissue inflammation.
Am J Physiol Endocrinol Metab. 303: E597-606. -
Akbarshahi, H. et al. (2012) Enrichment of Murine CD68(+)CCR2(+) and CD68(+)CD206(+) Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.
PLoS One. 7: e42654. -
Banda NK et al. (2012) Role of C3a receptors, C5a receptors, and complement protein C6 deficiency in collagen antibody-induced arthritis in mice.
J Immunol. 188 (3): 1469-78. -
Bonde, A.K. et al. (2012) Intratumoral macrophages contribute to epithelial-mesenchymal transition in solid tumors.
BMC Cancer. 12: 35. -
Choi, K.M. et al. (2010) CD206-positive M2 macrophages that express heme oxygenase-1 protect against diabetic gastroparesis in mice.
Gastroenterology. 138 (7): 2399-409, 2409.e1. -
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Our F4/80 (Cl:A3-1) Antibody has been referenced in >719 publications*
*Based on June 2020 data from CiteAb's antibody search engine.
