ER-MP58 antibody | ER-MP58
Rat anti Mouse ER-MP58 antibody, clone ER-MP58 (MCA2391) used to demonstrate recruited macrophages in atherosclerotic lesions by immunohistochemistry on acetone fixed sections.
Image caption:
Increased leukocyte adhesion and newly recruited myeloid cells in IκBαdel mice. (A) In IκBαdel mice, more leukocytes adhere to the endothelial cell layer delineating the lesion (*** p<0.0001; n = 17/17). Representative pictures from (B) IκBαWT and (C) IκBαdel lesions, original magnification ×200. (D) Lesions from IκBαdel mice contain more ER-MP58 positive cells, suggesting an increased attraction myeloid cells (** p<0.001; n = 19/17). Representative pictures from (E) IκBαWT and (F) IκBαdel lesions, original magnification ×200.
From: Goossens P, Vergouwe MN, Gijbels MJJ, Curfs DMJ, van Woezik JHG, Hoeksema MA, et al. (2011)
Myeloid IκBα Deficiency Promotes Atherogenesis by Enhancing Leukocyte Recruitment to the Plaques.
PLoS ONE 6(7): e22327.
Rat anti Mouse ER-MP58 antibody, clone ER-MP58 recognizes the murine antigen ER-MP58, which is expressed by all bone marrow-derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors.
The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down-regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid-committed cells and other haematopoietic progenitors cells in the BM. The antigen has been used as a marker of murine macrophage development in the BM.
ER-MP58 is suitable for the identification of myeloid haemopoietic islands in various organs, and for embryonal studies.
The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down-regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid-committed cells and other haematopoietic progenitors cells in the BM. The antigen has been used as a marker of murine macrophage development in the BM.
ER-MP58 is suitable for the identification of myeloid haemopoietic islands in various organs, and for embryonal studies.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Balb/c macrophage precursor cell hybrids
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of ER-MP58 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/25 | 1/100 | |
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Product Specific References
References for ER-MP58 antibody
-
Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
Eur J Immunol. 20 (1): 27-34. -
Henkel, G.W. et al. (1999) Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1.
Blood. 93 (9): 2849-58. -
Nikolic, T. et al. (2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.
Int Immunol. 15 (4): 515-24. -
Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
J Leukoc Biol. 78 (4): 845-52. -
Sunderkötter, C. et al. (2004) Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response.
J Immunol. 172: 4410-7. -
Chan, J. et al., (1998) Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression.
Blood. 1998 Aug 92: 1423-31. -
Oomen, S.P. et al. (2002) Somatostatin is a selective chemoattractant for primitive (CD34(+)) hematopoietic progenitor cells.
Exp Hematol. 30: 116-25. -
Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.
Am J Pathol. 158: 131-45. -
Rössner, S. et al. (2005) Myeloid dendritic cell precursors generated from bone marrow suppress T cell responses via cell contact and nitric oxide production in vitro.
Eur J Immunol. 35: 3533-44. -
Goossens, P. et al. (2011) Myeloid IκBα deficiency promotes atherogenesis by enhancing leukocyte recruitment to the plaques.
PLoS One. 6: e22327. -
Iwasaki, Y. et al. (2011) In situ proliferation and differentiation of macrophages in dental pulp.
Cell Tissue Res. 346: 99-109. -
Mossink, M.H. et al. (2003) Unimpaired dendritic cell functions in MVP/LRP knockout mice.
Immunology. 110: 58-65. -
Oliveira, M.A. et al. (2003) Immature macrophages derived from mouse bone marrow produce large amounts of IL-12p40 after LPS stimulation.
J Leukoc Biol. 74: 857-67. -
de Bruijn, M.F. et al. (1996) High-level expression of the ER-MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage.
Eur J Immunol. 26: 2850-8. -
Welzen-Coppens, J.M. et al. (2012) Abnormalities of dendritic cell precursors in the pancreas of the NOD mouse model of diabetes.
Eur J Immunol. 42: 186-94. -
Hoeksema, M.A. et al. (2014) Targeting macrophage Histone deacetylase 3 stabilizes atherosclerotic lesions.
EMBO Mol Med. pii: e201404170.
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