ER-MP58 antibody | ER-MP58

Rat anti Mouse ER-MP58

Product Type
Monoclonal Antibody
Clone
ER-MP58
Isotype
IgG2a
Specificity
ER-MP58

Product Code Applications Pack Size List Price Your Price Qty
MCA2391
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP 0.25 mg loader
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loader
MCA2391T
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SDS Safety Datasheet SDS
C F IP 25 µg loader
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loader
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Rat anti Mouse ER-MP58 antibody, clone ER-MP58 recognizes the murine antigen ER-MP58, which is expressed by all bone marrow-derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors.

The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down-regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid-committed cells, haematopoietic progenitors cells and as a marker for macrophage development in bone marrow. ER-MP58 is suitable for the identification of myeloid haemopoietic islands in various organs, and for embryonic tissues.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Balb/c macrophage precursor cell hybrids
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunized rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen 1/25 1/100
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rat IgG2a Negative Control

References for ER-MP58 antibody

  1. Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
    Eur J Immunol. 20 (1): 27-34.
  2. Henkel, G.W. et al. (1999) Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1.
    Blood. 93 (9): 2849-58.
  3. Nikolic, T. et al. (2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.
    Int Immunol. 15 (4): 515-24.
  4. Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
    J Leukoc Biol. 78 (4): 845-52.
  5. Sunderkötter, C. et al. (2004) Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response.
    J Immunol. 172: 4410-7.
  6. Chan, J. et al., (1998) Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression.
    Blood. 1998 Aug 92: 1423-31.
  7. Oomen, S.P. et al. (2002) Somatostatin is a selective chemoattractant for primitive (CD34(+)) hematopoietic progenitor cells.
    Exp Hematol. 30: 116-25.
  8. Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.
    Am J Pathol. 158: 131-45.
  9. View The Latest Product References
  10. Rössner, S. et al. (2005) Myeloid dendritic cell precursors generated from bone marrow suppress T cell responses via cell contact and nitric oxide production in vitro.
    Eur J Immunol. 35: 3533-44.
  11. Goossens, P. et al. (2011) Myeloid IκBα deficiency promotes atherogenesis by enhancing leukocyte recruitment to the plaques.
    PLoS One. 6: e22327.
  12. Iwasaki, Y. et al. (2011) In situ proliferation and differentiation of macrophages in dental pulp.
    Cell Tissue Res. 346: 99-109.
  13. Hoeksema, M.A. et al. (2014) Targeting macrophage Histone deacetylase 3 stabilizes atherosclerotic lesions.
    EMBO Mol Med. pii: e201404170.
  14. Oliveira, M.A. et al. (2003) Immature macrophages derived from mouse bone marrow produce large amounts of IL-12p40 after LPS stimulation.
    J Leukoc Biol. 74: 857-67.
  15. de Bruijn, M.F. et al. (1996) High-level expression of the ER-MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage.
    Eur J Immunol. 26: 2850-8.
  16. Welzen-Coppens, J.M. et al. (2012) Abnormalities of dendritic cell precursors in the pancreas of the NOD mouse model of diabetes.
    Eur J Immunol. 42: 186-94.
  17. Neele, A.E. et al. (2018) Myeloid Kdm6b deficiency results in advanced atherosclerosis.
    Atherosclerosis. 275: 156-165.
  18. Luque-Martin, R. et al. (2019) Targeting Histone Deacetylases in Myeloid Cells Inhibits Their Maturation and Inflammatory Function With Limited Effects on Atherosclerosis.
    Front Pharmacol. 10: 1242.

Immunohistology - Frozen

Synonyms
Myeloid Precursor Antigen
RRID
AB_931727
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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