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ER-MP58 antibody | ER-MP58

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Anti Mouse ER-MP58 Antibody, clone ER-MP58 gallery image 1
Published Investigator image:
Rat anti Mouse ER-MP58 antibody, clone ER-MP58 (MCA2391) used to demonstrate recruited macrophages in atherosclerotic lesions by immunohistochemistry on acetone fixed sections.
Image caption:
Increased leukocyte adhesion and newly recruited myeloid cells in IκBαdel mice. (A) In IκBαdel mice, more leukocytes adhere to the endothelial cell layer delineating the lesion (*** p<0.0001; n = 17/17). Representative pictures from (B) IκBαWT and (C) IκBαdel lesions, original magnification ×200. (D) Lesions from IκBαdel mice contain more ER-MP58 positive cells, suggesting an increased attraction myeloid cells (** p<0.001; n = 19/17). Representative pictures from (E) IκBαWT and (F) IκBαdel lesions, original magnification ×200.

From: Goossens P, Vergouwe MN, Gijbels MJJ, Curfs DMJ, van Woezik JHG, Hoeksema MA, et al. (2011)
Myeloid IκBα Deficiency Promotes Atherogenesis by Enhancing Leukocyte Recruitment to the Plaques.
PLoS ONE 6(7): e22327.

Rat anti Mouse ER-MP58

Product Type
Monoclonal Antibody
Clone
ER-MP58
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2391
Datasheet
C F IF IP
SDS
0.25 mg loader

MCA2391T
Datasheet
C F IP
SDS
25 µg loader

Rat anti Mouse ER-MP58 antibody, clone ER-MP58 recognizes the murine antigen ER-MP58, which is expressed by all bone marrow-derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors.

The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down-regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid-committed cells and other haematopoietic progenitors cells in the BM. The antigen has been used as a marker of murine macrophage development in the BM.

ER-MP58 is suitable for the identification of myeloid haemopoietic islands in various organs, and for embryonal studies.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Balb/c macrophage precursor cell hybrids
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunized rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of ER-MP58 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen 1/25 1/100
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

References for ER-MP58 antibody

  1. Leenen, P.J. et al. (1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.
    Eur J Immunol. 20 (1): 27-34.
  2. Henkel, G.W. et al. (1999) Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1.
    Blood. 93 (9): 2849-58.
  3. Nikolic, T. et al. (2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.
    Int Immunol. 15 (4): 515-24.
  4. Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.
    J Leukoc Biol. 78 (4): 845-52.
  5. Sunderkötter, C. et al. (2004) Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response.
    J Immunol. 172: 4410-7.
  6. Chan, J. et al., (1998) Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression.
    Blood. 1998 Aug 92: 1423-31.
  7. Oomen, S.P. et al. (2002) Somatostatin is a selective chemoattractant for primitive (CD34(+)) hematopoietic progenitor cells.
    Exp Hematol. 30: 116-25.
  8. Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.
    Am J Pathol. 158: 131-45.
  9. Rössner, S. et al. (2005) Myeloid dendritic cell precursors generated from bone marrow suppress T cell responses via cell contact and nitric oxide production in vitro.
    Eur J Immunol. 35: 3533-44.
  10. Goossens, P. et al. (2011) Myeloid IκBα deficiency promotes atherogenesis by enhancing leukocyte recruitment to the plaques.
    PLoS One. 6: e22327.
  11. Iwasaki, Y. et al. (2011) In situ proliferation and differentiation of macrophages in dental pulp.
    Cell Tissue Res. 346: 99-109.
  12. Mossink, M.H. et al. (2003) Unimpaired dendritic cell functions in MVP/LRP knockout mice.
    Immunology. 110: 58-65.
  13. Oliveira, M.A. et al. (2003) Immature macrophages derived from mouse bone marrow produce large amounts of IL-12p40 after LPS stimulation.
    J Leukoc Biol. 74: 857-67.
  14. de Bruijn, M.F. et al. (1996) High-level expression of the ER-MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage.
    Eur J Immunol. 26: 2850-8.
  15. Welzen-Coppens, J.M. et al. (2012) Abnormalities of dendritic cell precursors in the pancreas of the NOD mouse model of diabetes.
    Eur J Immunol. 42: 186-94.
  16. Hoeksema, M.A. et al. (2014) Targeting macrophage Histone deacetylase 3 stabilizes atherosclerotic lesions.
    EMBO Mol Med. pii: e201404170.

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