Delta-Like Protein 1 antibody | HMD1-5
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Hamster anti Mouse Delta-Like Protein 1
- Product Type
- Monoclonal Antibody
- Delta-Like Protein 1
|Hamster anti Mouse Delta-Like Protein 1 antibody, clone HMD1-5 recognizes Delta-like protein 1 (DLL1), one of the five major ligands of the Notch signaling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4.
The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression.
Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.
DLL1 is widely expressed, and acts as a mediator of cell fate decisions during hematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signaling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB).
Hamster anti Mouse Delta-Like Protein 1 antibody, clone HMD1-5 blocks binding of Notch2 to Dll1 (Moriyama et al. 2008)
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat Human Weak
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- DLL1-expressing CHO cells.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Functional Assays 1|
|Immunohistology - Frozen|
- 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Mouse spleen
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Hamster (Armenian) IgG Negative Control||MCA2356||F||0.25 mg||Log in|
|List Price||Your Price|
|Description||Hamster (Armenian) IgG Negative Control|
References for Delta-Like Protein 1 antibody
Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice.
Int Immunol. 20 (6): 763-73.
Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands.
Int Immunol. 21 (3): 295-301.
Sekine, C. et al. (2012) Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.
Arthritis Res Ther. 14: R45.
Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
Nat Rev Mol Cell Biol. 7 (9): 678-89.
Iso, T. et al. (2003) Notch signaling in vascular development.
Arterioscler Thromb Vasc Biol. 23 (4): 543-53.
Hu, X. et al. (2008) Integrated regulation of Toll-like receptor responses by Notch and interferon-gamma pathways.
Immunity. 29 (5): 691-703.
Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity.
Immunol Rev. 182: 215-27.
- Entrez Gene
- GO Terms
- GO:0005509 calcium ion binding
- GO:0001709 cell fate determination
- GO:0005576 extracellular region
- GO:0005887 integral to plasma membrane
- GO:0005112 Notch binding
- GO:0007154 cell communication
- GO:0007219 Notch signaling pathway
- GO:0007220 Notch receptor processing
- GO:0030097 hemopoiesis
- View More GO Terms
- GO:0030155 regulation of cell adhesion
- GO:0016021 integral to membrane
- GO:0009912 auditory receptor cell fate commitment
- GO:0005886 plasma membrane
- GO:0001701 in utero embryonic development
- GO:0001757 somite specification
- GO:0007368 determination of left/right symmetry
- GO:0007386 compartment pattern formation
- GO:0031410 cytoplasmic vesicle
- GO:0042472 inner ear morphogenesis
- GO:0042475 odontogenesis of dentine-containing tooth
- GO:0045608 negative regulation of auditory receptor cell differentiation
MCA5705GA147828 163064 1807
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