Delta-Like Protein 1 antibody | HMD1-5

Hamster anti Mouse Delta-Like Protein 1

Product Type
Monoclonal Antibody
Clone
HMD1-5
Isotype
IgG
Specificity
Delta-Like Protein 1

Product Code Applications Pack Size List Price Your Price Qty
MCA5705GA
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SDS Safety Datasheet SDS
C F FN * 0.1 mg loader
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MCA5705
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SDS Safety Datasheet SDS
C F FN * 0.25 mg loader
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Hamster anti Mouse Delta-Like Protein 1 antibody, clone HMD1-5 recognizes Delta-like protein 1 (DLL1), one of the five major ligands of the Notch signaling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4.

The Notch signaling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T-cell development, and both tumor progression and suppression.

Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta-like protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and the gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signaling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.

DLL1 is widely expressed, and acts as a mediator of cell fate decisions during hematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signaling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB).

Hamster anti Mouse Delta-Like Protein 1 antibody, clone HMD1-5 blocks binding of Notch2 to Dll1 (Moriyama et al. 2008)

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human Weak
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
DLL1-expressing CHO cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Functional Assays 1
Immunohistology - Frozen
  1. 1 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Mouse spleen

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Description Goat anti Hamster IgG:HRP
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Description Goat F(ab')2 anti Hamster IgG:DyLight®550
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Description Goat F(ab')2 anti Hamster IgG:DyLight®650

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References for Delta-Like Protein 1 antibody

  1. Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice.
    Int Immunol. 20 (6): 763-73.
  2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands.
    Int Immunol. 21 (3): 295-301.
  3. Sekine, C. et al. (2012) Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.
    Arthritis Res Ther. 14: R45.

Further Reading

  1. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
    Nat Rev Mol Cell Biol. 7 (9): 678-89.
  2. Iso, T. et al. (2003) Notch signaling in vascular development.
    Arterioscler Thromb Vasc Biol. 23 (4): 543-53.
  3. Hu, X. et al. (2008) Integrated regulation of Toll-like receptor responses by Notch and interferon-gamma pathways.
    Immunity. 29 (5): 691-703.
  4. Hoyne, G.F. et al. (2001) Notch signalling in the regulation of peripheral immunity.
    Immunol Rev. 182: 215-27.

Synonyms
DLL1
RRID
AB_10708982
UniProt
Q61483
P97677
O00548
Entrez Gene
DLL1
Dll1
Dll1
GO Terms
GO:0005509 calcium ion binding
GO:0001709 cell fate determination
GO:0005576 extracellular region
GO:0005887 integral to plasma membrane
GO:0005112 Notch binding
GO:0007154 cell communication
GO:0007219 Notch signaling pathway
GO:0007220 Notch receptor processing
GO:0030097 hemopoiesis
GO:0030155 regulation of cell adhesion
GO:0016021 integral to membrane
GO:0009912 auditory receptor cell fate commitment
GO:0005886 plasma membrane
GO:0001701 in utero embryonic development
GO:0001757 somite specification
GO:0007368 determination of left/right symmetry
GO:0007386 compartment pattern formation
GO:0031410 cytoplasmic vesicle
GO:0042472 inner ear morphogenesis
GO:0042475 odontogenesis of dentine-containing tooth
GO:0045608 negative regulation of auditory receptor cell differentiation
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