CDw210a antibody | 1B1.3a
Rat anti Mouse CDw210a
- Product Type
- Monoclonal Antibody
|Rat anti Mouse CDw210a antibody, clone 1B1.3a recognizes mouse CDw210a, a cell surface glycoprotein of approximately 110 kDa, also known as Interleukin-10 receptor 1 (IL-10R1). The epitope recognized by this antibody has been localised to the ligand binding domain within the extracellular region of mouse CDw210a. Mouse CDw210a is expressed by a range of cells including thymocytes, T cells, B cells and monocytes. Rat anti Mouse CDw210a antibody, clone 1B1.3a is reported to neutralize the effects of IL-10 (Asseman et al. 1997).|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Human
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Purified recombinant extracellular region of mouse CDw210a.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Fisher rats were fused with cells of the mouse SP2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
References for CDw210a antibody
Asseman, C. et al. (1999) An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation.
J Exp Med. 190 (7): 995-1004.
Phillips, J.M. et al. (2001) Cutting edge: interactions through the IL-10 receptor regulate autoimmune diabetes.
J Immunol. 167 (11): 6087-91.
Iwashiro, M. et al. (2001) Immunosuppression by CD4+ regulatory T cells induced by chronic retroviral infection.
Proc Natl Acad Sci U S A. 98: 9226-30.
Montagnoli, C. et al. (2002) B7/CD28-dependent CD4+CD25+ regulatory T cells are essential components of the memory-protective immunity to Candida albicans.
J Immunol. 169: 6298-308.
Vicari, A.P. et al. (2002) Reversal of tumor-induced dendritic cell paralysis by CpG immunostimulatory oligonucleotide and anti-interleukin 10 receptor antibody.
J Exp Med. 196: 541-9.
Sundstedt, A. et al. (2003) Role for IL-10 in suppression mediated by peptide-induced regulatory T cells in vivo.
J Immunol. 170 (3): 1240-8.
Asseman, C. et al. (2003) Colitogenic Th1 cells are present in the antigen-experienced T cell pool in normal mice: control by CD4+ regulatory T cells and IL-10.
J Immunol. 171: 971-8.
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