CDw210a antibody | 1B1.3a
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Rat anti Mouse CDw210a antibody, clone 1B1.3a recognizes mouse CDw210a, a cell surface glycoprotein of approximately 110 kDa, also known as Interleukin-10 receptor 1 (IL-10R1). The epitope recognized by this antibody has been localised to the ligand binding domain within the extracellular region of mouse CDw210a. Mouse CDw210a is expressed by a range of cells including thymocytes, T cells, B cells and monocytes. Rat anti Mouse CDw210a antibody, clone 1B1.3a is reported to neutralize the effects of IL-10 (Asseman et al. 1997).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Purified recombinant extracellular region of mouse CDw210a.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Fisher rats were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- Q61727 Related reagents
- Entrez Gene
- Il10ra Related reagents
- GO Terms
- GO:0007596 blood coagulation
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- Regulatory
- For research purposes only
Applications of CDw210a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | 1/10 | 1/100 | |
| Functional Assays | |||
| Flow Cytometry 1 | 1/10 | 1/100 |
- 1Due to low expression of CDw210a, sensitive staining techniques may be required.
- 1Due to low expression of CDw210a, sensitive staining techniques may be required.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Product Specific References
References for CDw210a antibody
-
Asseman, C. et al. (1999) An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation.
J Exp Med. 190 (7): 995-1004. -
Phillips, J.M. et al. (2001) Cutting edge: interactions through the IL-10 receptor regulate autoimmune diabetes.
J Immunol. 167 (11): 6087-91. -
Sundstedt, A. et al. (2003) Role for IL-10 in suppression mediated by peptide-induced regulatory T cells in vivo.
J Immunol. 170 (3): 1240-8. -
Asseman, C. et al. (2003) Colitogenic Th1 cells are present in the antigen-experienced T cell pool in normal mice: control by CD4+ regulatory T cells and IL-10.
J Immunol. 171: 971-8. -
Iwashiro, M. et al. (2001) Immunosuppression by CD4+ regulatory T cells induced by chronic retroviral infection.
Proc Natl Acad Sci U S A. 98: 9226-30. -
Montagnoli, C. et al. (2002) B7/CD28-dependent CD4+CD25+ regulatory T cells are essential components of the memory-protective immunity to Candida albicans.
J Immunol. 169: 6298-308. -
Vicari, A.P. et al. (2002) Reversal of tumor-induced dendritic cell paralysis by CpG immunostimulatory oligonucleotide and anti-interleukin 10 receptor antibody.
J Exp Med. 196: 541-9.
