CD94 antibody | 18d3
Rat anti Mouse CD94 antibody, clone 18d3 recognizes murine CD94, a ~70 kDa cell surface glycoprotein expressed primarily by mouse natural killer (NK) cells. The murine CD94 molecule forms heterodimers with NKG2A, C or E subunits to form the CD94/NKG2 receptor.
In mice the CD94/NKG2 receptor recognizes Qa-1b, a non-classical MHC class 1 molecule. CD94/NKG2 receptors may play an important role in self-tolerance of NK cells during fetal and neonatal life.
N.B. Recent reports suggest that DBA/2J mice do not express cell surface CD94/NKG2A receptors (Vance et al. 2002).
In mice the CD94/NKG2 receptor recognizes Qa-1b, a non-classical MHC class 1 molecule. CD94/NKG2 receptors may play an important role in self-tolerance of NK cells during fetal and neonatal life.
N.B. Recent reports suggest that DBA/2J mice do not express cell surface CD94/NKG2A receptors (Vance et al. 2002).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- CHO cells transfected with B6 allele of CD94.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O54707
- Entrez Gene
- Klrd1
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005529 sugar binding
- GO:0009897 external side of plasma membrane
- Regulatory
- For research purposes only
Applications of CD94 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
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| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Product Specific References
References for CD94 antibody
-
Vance, R.E. et al. (2002) Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation.
Proc Natl Acad Sci U S A. 99 (2): 868-73. -
Vance, R.E. et al. (1999) Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells.
J Exp Med. 190 (12): 1801-12. -
Chattopadhyay, S. et al. (2008) Implication for the CD94/NKG2A-Qa-1 system in the generation and function of ocular-induced splenic CD8+ regulatory T cells.
Int Immunol. 20 (4): 509-16. -
Kawamura, H. et al. (2005) Amelioration of acute graft-versus-host disease by NKG2A engagement on donor T cells.
Eur J Immunol. 35 (8): 2358-66. -
Fraser, K.P. et al. (2002) NK cells developing in vitro from fetal mouse progenitors express at least one member of the Ly49 family that is acquired in a time-dependent and stochastic manner independently of CD94 and NKG2.
Eur J Immunol. 32 (3): 868-78.
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