CD94 antibody | 18d3

Product Code Applications Pack Size List Price Your Price Qty
MCA2288GA
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F 0.1 mg loader
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Rat anti Mouse CD94 antibody, clone 18d3 recognizes murine CD94, a ~70 kDa cell surface glycoprotein expressed primarily by mouse natural killer (NK) cells. The murine CD94 molecule forms heterodimers with NKG2A, C or E subunits to form the CD94/NKG2 receptor.
In mice the CD94/NKG2 receptor recognizes Qa-1b, a non-classical MHC class 1 molecule. CD94/NKG2 receptors may play an important role in self-tolerance of NK cells during fetal and neonatal life.
N.B. Recent reports suggest that DBA/2J mice do not express cell surface CD94/NKG2A receptors (Vance et al. 2002).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
CHO cells transfected with B6 allele of CD94.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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Description Rat IgG2a Negative Control

References for CD94 antibody

  1. Vance, R.E. et al. (2002) Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation.
    Proc Natl Acad Sci U S A. 99 (2): 868-73.
  2. Vance, R.E. et al. (1999) Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells.
    J Exp Med. 190 (12): 1801-12.
  3. Chattopadhyay, S. et al. (2008) Implication for the CD94/NKG2A-Qa-1 system in the generation and function of ocular-induced splenic CD8+ regulatory T cells.
    Int Immunol. 20 (4): 509-16.
  4. Kawamura, H. et al. (2005) Amelioration of acute graft-versus-host disease by NKG2A engagement on donor T cells.
    Eur J Immunol. 35 (8): 2358-66.
  5. Fraser, K.P. et al. (2002) NK cells developing in vitro from fetal mouse progenitors express at least one member of the Ly49 family that is acquired in a time-dependent and stochastic manner independently of CD94 and NKG2.
    Eur J Immunol. 32 (3): 868-78.

Flow Cytometry

RRID
AB_566930
UniProt
O54707
Entrez Gene
Klrd1
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005529 sugar binding
GO:0009897 external side of plasma membrane
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