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CD64 antibody | AT152-9

Product Code Applications Pack Size List Price Your Price Qty
MCA5997
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SDS Safety Datasheet SDS
C F 0.25 mg loader
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loader

Rat anti mouse CD64 antibody, clone AT152-9, recognizes mouse CD64 also known as FcRI. CD64 is a high affinity activatory receptor for IgG2a and a low affinity receptor for IgG2b and IgG3 type antibodies. The interaction between Fc receptors and antibodies play important roles in both the innate and adaptive immune responses.

CD64, through binding of the Fc segment of IgG, mediates phagocytosis and plays a role in antibody-dependent cellular cytotoxicity and clearance of immune complexes. In addition, CD64 also functions as an antigen capture for presentation to T-cells and also mediates the release of cytokines and reactive oxygen intermediates including interleukin (IL)-1, IL-6 and tumor necrosis factor (TNF) alpha. It is constitutively expressed on monocytes and macrophages, germinal centre dendritic cells and early myeloid lineage cells, but not lymphocytes. Expression on monocytes can be strongly upregulated by treatment with interferon (IFN) gamma or G-CSF, and can be induced on neutrophils and eosinophils by IFN gamma.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
HEK293F cells expressing CD64 and gamma chain
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of the NS-1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10
Immunohistology - Frozen 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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Description Rat IgG2a Negative Control

References for CD64 antibody

  1. White; A.L. et al. (2011) Interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 monoclonal antibody.
    J Immunol. 187 (4): 1754-63.
  2. Tutt AL et al. (2015) Development and Characterization of Monoclonal Antibodies Specific for Mouse and Human Fcγ Receptors.
    J Immunol. 195 (11): 5503-16.

Synonyms
FcRI
RRID
AB_2687456
UniProt
P26151
Entrez Gene
Fcgr1
GO Terms
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001805 positive regulation of type III hypersensitivity
GO:0042742 defense response to bacterium
GO:0016021 integral to membrane
GO:0006898 receptor-mediated endocytosis
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0019770 IgG receptor activity
GO:0042590 antigen processing and presentation of exogenous peptide antigen via MHC class I
GO:0045087 innate immune response
GO:0050766 positive regulation of phagocytosis

MCA5997

159055 161018

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