CD62L antibody | MEL-14

Rat anti Mouse CD62L

Product Type
Monoclonal Antibody
Clone
MEL-14
Isotype
IgG2a
Specificity
CD62L

Product Code Applications Pack Size List Price Your Price Qty
MCA1259G
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SDS Safety Datasheet SDS
C F IP 0.25 mg loader
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loader
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Rat anti mouse CD62L, clone MEL-14, recognizes mouse L-selectin. L-selectin is an adhesion molecule expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils, (Fink, P. et al., 1985).

Pre-incubation of lymphocytes with MEL-14 completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and the migration of lymphocytes to lymph nodes in vivo (Gallatin, W. et al. 1983). Removal of sodium azide is recommended prior to using this clone in functional assays.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Mouse B cell lymphoma, 38C-14.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Fischer rats were fused with cells of the mouse P3.X63.Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1ug/ml
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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Description Rat IgG2a Negative Control

References for CD62L antibody

  1. Fink, P.J. et al. (1985) Homing receptor-bearing thymocytes, an immunocompetent cortical subpopulation.
    Nature. 313 (5999): 233-5.
  2. Gallatin, W.M. et al. (1983) A cell-surface molecule involved in organ-specific homing of lymphocytes.
    Nature. 304 (5921): 30-4.
  3. Lewinsohn, D.M. et al. (1987) Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes.
    J Immunol. 138 (12): 4313-21.
  4. Reichert, R.A. et al. (1984) A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes.
    Cell. 38 (1): 89-99.
  5. Siegelman, M.H. et al. (1990) The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding.
    Cell. 61 (4): 611-22.
  6. Jalkanen, S. et al. (1987) Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells.
    J Cell Biol. 105 (2): 983-90.

Synonyms
LECAM-1
L-SELECTIN
RRID
AB_323637
UniProt
P18337
Entrez Gene
Sell
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0050839 cell adhesion molecule binding
GO:0005529 sugar binding
GO:0009897 external side of plasma membrane
GO:0033198 response to ATP
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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