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CD62L antibody | MEL-14

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Anti Mouse CD62L Antibody, clone MEL-14 thumbnail image 1
Anti Mouse CD62L Antibody, clone MEL-14 gallery image 1
Figure A. FITC conjugated rat anti mouse CD3 (MCA500F) and RPE conjugated rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated rat anti mouse CD3 (MCA500F) and RPE conjugated rat anti mouse CD62L (MCA1259PE). All experiments performed on red cell lysed mouse blood gated on lymphocytes in the presence of mouse seroblock (BUF041B). Data acquired on the ZE5 Cell Analyzer.

Rat anti Mouse CD62L:RPE

Product Type
Monoclonal Antibody
Clone
MEL-14
Isotype
IgG2a
Specificity
CD62L

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Product Code Applications Pack Size List Price Your Price Qty
MCA1259PE
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SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
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Rat anti Mouse CD62L, clone MEL-14, recognizes mouse L-selectin. L-selectin is an adhesion molecule expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils, (Fink, P. et al., 1985).

Pre-incubation of lymphocytes with MEL-14 completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and the migration of lymphocytes to lymph nodes in vivo (Gallatin et al. 1983). Removal of sodium azide is recommended prior to using this clone in functional assays.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Mouse B cell lymphoma, 38C-14.
Fusion Partners
Spleen cells from immunized Fischer rats were fused with cells of the mouse P3.X63.Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/2
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

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Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests
>
List Price Your Price
Description Rat IgG2a Negative Control:RPE

References for CD62L antibody

  1. Gallatin, W.M. et al. (1983) A cell-surface molecule involved in organ-specific homing of lymphocytes.
    Nature. 304 (5921): 30-4.
  2. Reichert, R.A. et al. (1984) A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes.
    Cell. 38 (1): 89-99.
  3. Fink, P.J. et al. (1985) Homing receptor-bearing thymocytes, an immunocompetent cortical subpopulation.
    Nature. 313 (5999): 233-5.
  4. Jalkanen, S. et al. (1987) Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells.
    J Cell Biol. 105 (2): 983-90.
  5. Lewinsohn, D.M. et al. (1987) Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes.
    J Immunol. 138 (12): 4313-21.
  6. Siegelman, M.H. et al. (1990) The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding.
    Cell. 61 (4): 611-22.

Synonyms
LECAM-1
L-SELECTIN
RRID
AB_324106
UniProt
P18337
Entrez Gene
Sell
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0050839 cell adhesion molecule binding
GO:0005529 sugar binding
GO:0009897 external side of plasma membrane
GO:0033198 response to ATP

MCA1259PE

154372

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