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CD62L antibody | MEL-14

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Anti Mouse CD62L Antibody, clone MEL-14 gallery image 1
Figure A. FITC conjugated rat anti mouse CD3 (MCA500F) and RPE conjugated rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated rat anti mouse CD3 (MCA500F) and RPE conjugated rat anti mouse CD62L (MCA1259PE). All experiments performed on red cell lysed mouse blood gated on lymphocytes in the presence of mouse seroblock (BUF041B). Data acquired on the ZE5™ Cell Analyzer.

Rat anti Mouse CD62L

Rat anti Mouse CD62L:RPE

Product Type
Monoclonal Antibody
Clone
MEL-14
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1259G
Datasheet
C F IP
SDS
0.25 mg loader

MCA1259PE
Datasheet
F
SDS
100 Tests loader

Rat anti mouse CD62L, clone MEL-14, recognizes mouse L-selectin. L-selectin is an adhesion molecule expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils, (Fink, P. et al., 1985).

Pre-incubation of lymphocytes with MEL-14 completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and the migration of lymphocytes to lymph nodes in vivo (Gallatin, W. et al. 1983). Removal of sodium azide is recommended prior to using this clone in functional assays. Bio-Rad recommend a Slide-A-Lyzer® Dialysis Cassette for this purpose.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites.
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Mouse B cell lymphoma, 38C-14.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Fischer rats were fused with cells of the mouse P3.X63.Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P18337
Entrez Gene
Sell
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0050839 cell adhesion molecule binding
GO:0005529 sugar binding
GO:0009897 external side of plasma membrane
GO:0033198 response to ATP
Regulatory
For research purposes only

Applications of CD62L antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1ug/ml
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat 1/2
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests loader

Product Specific References

References for CD62L antibody

  1. Fink, P.J. et al. (1985) Homing receptor-bearing thymocytes, an immunocompetent cortical subpopulation.
    Nature. 313 (5999): 233-5.
  2. Gallatin, W.M. et al. (1983) A cell-surface molecule involved in organ-specific homing of lymphocytes.
    Nature. 304 (5921): 30-4.
  3. Lewinsohn, D.M. et al. (1987) Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes.
    J Immunol. 138 (12): 4313-21.
  4. Reichert, R.A. et al. (1984) A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes.
    Cell. 38 (1): 89-99.
  5. Siegelman, M.H. et al. (1990) The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding.
    Cell. 61 (4): 611-22.
  6. Jalkanen, S. et al. (1987) Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells.
    J Cell Biol. 105 (2): 983-90.
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