CD62L antibody | MEL-14
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Rat anti Mouse CD62L:RPE
- Product Type
- Monoclonal Antibody
|Rat anti Mouse CD62L, clone MEL-14, recognizes mouse L-selectin. L-selectin is an adhesion molecule expressed on most T and B lymphocytes, neutrophils, monocytes and eosinophils, (Fink, P. et al., 1985).
Pre-incubation of lymphocytes with MEL-14 completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and the migration of lymphocytes to lymph nodes in vivo (Gallatin et al. 1983). Removal of sodium azide is recommended prior to using this clone in functional assays.
- Target Species
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitute with 1 ml distilled water
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- Mouse B cell lymphoma, 38C-14.
- Fusion Partners
- Spleen cells from immunized Fischer rats were fused with cells of the mouse P3.X63.Ag8.653 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578
- For research purposes only
- 12 months from date of despatch
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
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References for CD62L antibody
Gallatin, W.M. et al. (1983) A cell-surface molecule involved in organ-specific homing of lymphocytes.
Nature. 304 (5921): 30-4.
Reichert, R.A. et al. (1984) A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes.
Cell. 38 (1): 89-99.
Fink, P.J. et al. (1985) Homing receptor-bearing thymocytes, an immunocompetent cortical subpopulation.
Nature. 313 (5999): 233-5.
Jalkanen, S. et al. (1987) Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells.
J Cell Biol. 105 (2): 983-90.
Lewinsohn, D.M. et al. (1987) Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes.
J Immunol. 138 (12): 4313-21.
Siegelman, M.H. et al. (1990) The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding.
Cell. 61 (4): 611-22.
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