CD49d antibody | PS/2
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Rat anti Mouse CD49d
- Product Type
- Monoclonal Antibody
|Rat anti Mouse CD49d monoclonal antibody, clone PS/2 recognizes murine alpha 4 integrin (CD49d), a ~150 kDa single pass type I membrane glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively (Holzmann et al. 1989). CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1 (Sheppard et al. 1994).
Clone PS/2 has also been reported to block the binding of CD49d to its ligands (Andrew et al. 1994).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Human
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- P815 DBA/2 murine mastocytoma cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Fisher rats were fused with SP2/0 mouse myeloma cells
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD49d antibody
Miyake, K. et al. (1991) Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis.
J Exp Med. 173 (3): 599-607.
Miyake, K. et al. (1991) A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture.
J Cell Biol. 114 (3): 557-65.
Andrew, D.P. et al. (1994) Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation.
J Immunol. 153 (9): 3847-61.
Bellingan, G.J. et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation.
J Exp Med. 196: 1515-21.
Fukuoka, M. et al. (2000) Antiadhesive function of 130-kd glycoform of CD43 expressed in CD4 T-lymphocyte clones and transfectant cell lines.
Blood. 96: 4267-75.
Hokibara, S. et al. (2000) Effects of monoclonal antibodies to adhesion molecules on eosinophilic myocarditis in Toxocara canis-infected CBA/J mice.
Clin Exp Immunol. 114: 236-44.
Li, W. et al. (2008) Reduced alpha4beta1 integrin/VCAM-1 interactions lead to impaired pre-B cell repopulation in alpha 1,6-fucosyltransferase deficient mice.
Glycobiology. 18: 114-24.
Liu, Z.J. et al. (1999) A novel role for H-Ras in the regulation of very late antigen-4 integrin and VCAM-1 via c-Myc-dependent and -independent mechanisms.
J Immunol. 163: 4901-8.
View The Latest Product References
Tchilian, E.Z. et al. (1997) Anti-alpha 4 integrin antibody induces apoptosis in murine thymocytes and staphylococcal enterotoxin B-activated lymph node T cells.
Immunology. 92: 321-7.
Bowden, R.A. et al. (2002) Role of alpha4 integrin and VCAM-1 in CD18-independent neutrophil migration across mouse cardiac endothelium.
Circ Res. 90: 562-9.
Hirata, T. et al. (2002) P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin.
J Immunol. 169: 4307-13.
Vaz, R. et al. (2012) Fibronectin promotes migration, alignment and fusion in an in vitro myoblast cell model.
Cell Tissue Res. 348: 569-78.
Gillberg, L. et al. (2013) Effective treatment of mouse experimental colitis by alpha 2 integrin antibody: comparison with alpha 4 antibody and conventional therapy.
Acta Physiol (Oxf). 207: 326-36.
Eshghi, S. et al. (2007) Alpha4beta1 integrin and erythropoietin mediate temporally distinct steps in erythropoiesis: integrins in red cell development.
J Cell Biol. 177: 871-80.
Maus, U.A. et al. (2004) Pneumolysin-induced lung injury is independent of leukocyte trafficking into the alveolar space.
J Immunol. 173: 1307-12.
Enghofer, M. et al. (1998) Lymphocyte transfer in streptozotocin-induced diabetes: adhesion of donor cells to islet endothelium.
Am J Physiol. 274: E928-35.
Zhang, Y. et al. (2012) Autotaxin through lysophosphatidic acid stimulates polarization, motility, and transendothelial migration of naive T cells.
J Immunol. 189: 3914-24.
Ferrer, P. et al. (2005) Association between pterostilbene and quercetin inhibits metastatic activity of B16 melanoma.
Neoplasia. 7: 37-47.
Tanneau, G.M. et al. (1999) Differential recruitment of T- and IgA B-lymphocytes in the developing mammary gland in relation to homing receptors and vascular addressins.
J Histochem Cytochem. 47: 1581-92.
Omenetti, S. et al. (2015) Dysregulated intrahepatic CD4+ T-cell activation drives liver inflammation in ileitis-prone SAMP1/YitFc mice.
Cell Mol Gastroenterol Hepatol. 1 (4): 406-19.
Chung, K.J. et al. (2017) A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity.
Nat Immunol. 18 (6): 654-64.
- Integrin Alpha 4 Chain
- Entrez Gene
- GO Terms
- GO:0007157 heterophilic cell-cell adhesion
- GO:0001968 fibronectin binding
- GO:0001974 blood vessel remodeling
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007507 heart development
- GO:0009897 external side of plasma membrane
- View More GO Terms
- GO:0016477 cell migration
- GO:0060324 face development
- GO:0060710 chorio-allantoic fusion
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