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CD71 antibody | YTA74.4

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Rat anti Mouse CD71:StarBright Violet 610

Product Type
Monoclonal Antibody
Clone
YTA74.4
Isotype
IgG2a
Specificity
CD71

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Product Code Applications Pack Size List Price Your Price Qty
MCA1033SBV610
Datasheet Datasheet
SDS Safety Datasheet SDS
F * 100 Tests/0.5ml loader
List Price Your Price
loader
Anti Mouse CD71 Antibody, clone YTA74.4 thumbnail image 1 Anti Mouse CD71 Antibody, clone YTA74.4 thumbnail image 2 Anti Mouse CD71 Antibody, clone YTA74.4 thumbnail image 3
Anti Mouse CD71 Antibody, clone YTA74.4 gallery image 1
Figure A. StarBright UltraViolet 400 conjugated Rat anti Mouse CD45R antibody, clone RA3-6B2 (MCA1258SBUV400).
Figure B. StarBright UltraViolet 400 conjugated Rat anti Mouse CD45R antibody, clone RA3-6B2 (MCA1258SBUV400) and StarBright Violet 790 conjugated Rat anti Mouse CD71 antibody, clone YTA74.4 (​MCA1033SBV790). All experiments performed on mouse splenocytes, in the presence of 10% mouse serum.
Data acquired on the ZE5 Cell analyser.
Anti Mouse CD71 Antibody, clone YTA74.4 gallery image 2
Figure A. StarBright UltraViolet 400 conjugated Rat anti Mouse CD45R antibody, clone RA3-6B2 (MCA1258SBUV400).
Figure B. StarBright UltraViolet 400 conjugated Rat anti Mouse CD45R antibody, clone RA3-6B2 (MCA1258SBUV400) and StarBright Violet 610 conjugated Rat anti Mouse CD71 antibody, clone YTA74.4 (MCA1033SBV610). mouse splenocytes, in the presence of 10% mouse serum.
Data acquired on the ZE5 Cell analyser.
Anti Mouse CD71 Antibody, clone YTA74.4 gallery image 3
Published customer image:
Rat anti Mouse CD71 antibody, clone YTA74.1 (MCA1033G) used to evaluate TfR expression in mouse retinall tissue lysates by western blotting.
Image caption:
Tfr protein levels decreased in both the 2-week MCA (K) and 5-month MCA (L) retinas compared with age-matched controls, as assessed by Western analysis. Loading control β-actin (42 kDa) bands are shown below each set of lanes. Plots of densitometry normalized to loading control calculated using FIJI software. Numbers represent mean values (±SEM). *P <0.05, **P <0.01.

From: Baumann B, Sterling J, Song Y, Song D, Fruttiger M, Gillies M, Shen W, Dunaief JL.
Conditional Müller Cell Ablation Leads to Retinal Iron Accumulation.
Invest Ophthalmol Vis Sci. 2017 Aug 1;58(10):4223-4234.
doi: 10.1167/iovs.17-21743.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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F WB

Rat anti Mouse CD71 antibody, clone YTA74.4 recognizes the mouse transferrin receptor protein 1 also known as CD71 or TfR1. CD71 is a 763 amino acid glycoprotein homodimer of ~95 kDa subunits. CD71 is expressed by dividing cells, and functions as a transferrin receptor mediating uptake of iron.

Rat anti Mouse CD71 antibody, clone YTA74.4 blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (Trowbridge et al. 1982).

Target Species
Mouse
Product Form
Purified IgG conjugated to StarBright Violet 610 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Concanavilin A activated mouse spleen cells.
Approx. Protein Concentrations
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Fusion Partners
Spleen cells from an immunized DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 610 403 607
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
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Mouse Seroblock FcR BUF041B F 0.5 mg loader
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References for CD71 antibody

  1. Millot, S. et al. (2010) Erythropoietin stimulates spleen BMP4-dependent stress erythropoiesis and partially corrects anemia in a mouse model of generalized inflammation.
    Blood. 116: 6072-81.
  2. Kuo, Y.M. et al. (2004) Mislocalisation of hephaestin, a multicopper ferroxidase involved in basolateral intestinal iron transport, in the sex linked anaemia mouse.
    Gut. 53: 201-6.
  3. Krysiak, K. et al. (2015) Reduced levels of Hspa9 attenuate Stat5 activation in mouse B cells.
    Exp Hematol. 43 (4): 319-30.e10.
  4. Byun, M. et al. (2007) Cowpox virus exploits the endoplasmic reticulum retention pathway to inhibit MHC class I transport to the cell surface.
    Cell Host Microbe. 2: 306-15.
  5. Ripich, T. and Jessberger, R. (2011) SWAP-70 regulates erythropoiesis by controlling α4 integrin.
    Haematologica. 96: 1743-52.
  6. Hadziahmetovic, M. et al. (2012) Microarray analysis of murine retinal light damage reveals changes in iron regulatory, complement, and antioxidant genes in the neurosensory retina and isolated RPE.
    Invest Ophthalmol Vis Sci. 53 (9): 5231-41.
  7. Niewoehner, J. et al. (2014) Increased brain penetration and potency of a therapeutic antibody using a monovalent molecular shuttle.
    Neuron. 81: 49-60.
  8. Sands, S.A. et al. (2015) The habenula and iron metabolism in cerebral mouse models of multiple sclerosis.
    Neurosci Lett. 606: 204-8.
    9. View The Latest Product References
  9. Baumann, B. et al. (2017) Conditional Müller Cell Ablation Leads to Retinal Iron Accumulation.
    Invest Ophthalmol Vis Sci. 58 (10): 4223-34.
  10. Nelvagal, H.R. et al. (2020) Comparative proteomic profiling reveals mechanisms for early spinal cord vulnerability in CLN1 disease.
    Sci Rep. 10 (1): 15157.
  11. Hargreaves, A. et al. (2021) Tumors modulate fenestrated vascular beds and host endocrine status.
    J Appl Toxicol. 41 (12): 1952-65.
  12. Zhang, K.R. et al. (2022) Conditional knockout of hephaestin in the neural retina disrupts retinal iron homeostasis.
    Exp Eye Res. 218: 109028.
  13. Hargreaves, A. et al. (2022) Tumours modulate the systemic vascular response to anti-angiogenic therapy.
    J Appl Toxicol. 42 (8): 1371-84.
  14. Hargreaves, A. et al. (2021) Tumors modulate fenestrated vascular beds and host endocrine status.
    J Appl Toxicol. 41 (12): 1952-65.

Further Reading

  1. Lesley, J. et al. (1984) Expression of transferrin receptor on murine hematopoietic progenitors.
    Cell Immunol. 83 (1): 14-25.
  2. Trowbridge, I.S. et al. (1982) Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody.
    J Cell Physiol. 112 (3): 403-10.

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Western Blotting

Synonyms
Transferrin Receptor
RRID
AB_2943377
UniProt
Q62351
Entrez Gene
Tfrc
GO Terms
GO:0005886 plasma membrane
GO:0008233 peptidase activity
GO:0005381 iron ion transmembrane transporter activity
GO:0016021 integral to membrane
GO:0004998 transferrin receptor activity
GO:0005768 endosome
GO:0006508 proteolysis
GO:0006879 cellular iron ion homeostasis
GO:0006897 endocytosis
GO:0042470 melanosome
GO:0048471 perinuclear region of cytoplasm
GO:0070062 extracellular vesicular exosome

MCA1033SBV610

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