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CD45R antibody | RA3-6B2

Rat anti Mouse CD45R:FITC

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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Rat anti Mouse CD45R antibody, clone RA3-6B2 recognizes murine CD45R, a form of the CD45 antigen expressed by B cells and lytically active subsets of NK cells and non-MHC restricted CTL's. Rat anti Mouse CD45R antibody, clone RA3-6B2 immunoprecipitates the high molecular weight form of CD45 (220 kDa).

Rat anti Mouse CD45R antibody, clone RA3-6B2 is suitable for plp fixed paraffin embedded tissues (Whiteland et al.1995).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Murine leukemia-induced pre-B tumor cells (RAW112)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized Lewis rats were fused with cells of the rat S194/5 XX0.BU-1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
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Description Rat IgG2a Negative Control:FITC

Source Reference

  1. Coffman, R.L. (1982) Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development.
    Immunol Rev. 69: 5-23.

References for CD45R antibody

  1. Holmes, K.L. et al. (1986) Analysis of neoplasms induced by Cas-Br-M MuLV tumor extracts.
    J Immunol. 137 (2): 679-88.
  2. Spangrude, G.J. et al. (1988) Purification and characterization of mouse hematopoietic stem cells.
    Science. 241: 58-62.
  3. Spangrude, G.J. et al. (1988) Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus.
    J Exp Med. 167 (5): 1671-83.
  4. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  5. Hawke, S. et al. (1998) Long-term persistence of activated cytotoxic T lymphocytes after viral infection of the central nervous system.
    J Exp Med. 187: 1575-82.
  6. Rosmalen, J.G. et al. (2000) Subsets of macrophages and dendritic cells in nonobese diabetic mouse pancreatic inflammatory infiltrates: correlation with the development of diabetes.
    Lab Invest. 80 (1): 23-30.
  7. Perry, M.J. et al. (2000) Effects of high-dose estrogen on murine hematopoietic bone marrow precede those on osteogenesis.
    Am J Physiol Endocrinol Metab. 279: E1159-65.
  8. Stevenson, P.G. et al. (2002) Uncoupling of virus-induced inflammation and anti-viral immunity in the brain parenchyma.
    J Gen Virol. 83: 1735-43.
  9. View The Latest Product References
  10. Straubinger, R.K. et al. (2003) Quantitative evaluation of inflammatory and immune responses in the early stages of chronic Helicobacter pylori infection.
    Infect Immun. 71: 2693-703.
  11. Shulga-Morskaya, S. et al. (2004) B cell-activating factor belonging to the TNF family acts through separate receptors to support B cell survival and T cell-independent antibody formation.
    J Immunol. 173 (4): 2331-41.
  12. Gengozian, N. et al. (2005) Characterization of a monoclonal antibody identifying a CD45RA antigen on feline leukocytes.
    Vet Immunol Immunopathol. 108: 253-64.
  13. Herrmann, I. et al. (2006) Streptococcus pneumoniae Infection aggravates experimental autoimmune encephalomyelitis via Toll-like receptor 2.
    Infect Immun. 74: 4841-8.
  14. Itoh, T. et al. (2007) Ddb2 is a haploinsufficient tumor suppressor and controls spontaneous germ cell apoptosis.
    Hum Mol Genet. 16: 1578-86.
  15. McGill, J. et al. (2009) Fetal exposure to ethanol has long-term effects on the severity of influenza virus infections.
    J Immunol. 182: 7803-8
  16. Ankeny, D.P. et al. (2009) B cells produce pathogenic antibodies and impair recovery after spinal cord injury in mice.
    J Clin Invest. 119: 2990-9.
  17. Lacroix-Lamande, S. et al. (2009) Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation.
    PLoS One. 4: e8291.
  18. Lundqvist, J. et al. (2010) Concomitant infection decreases the malaria burden but escalates relapsing fever borreliosis.
    Infect Immun. 78 (5): 1924-30.
  19. Giuriato, S. et al. (2010) Conditional TPM3-ALK and NPM-ALK transgenic mice develop reversible ALK-positive early B-cell lymphoma/leukemia.
    Blood. 115: 4061-70.
  20. Kleiter, I. et al. (2010) Smad7 in T cells drives T helper 1 responses in multiple sclerosis and experimental autoimmune encephalomyelitis.
    Brain. 133: 1067-81.
  21. Nakaya, T. et al. (2010) Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression.
    J Immunol. 185: 5180-7.
  22. Soejima, M. et al. (2011) Role of innate immunity in a murine model of histidyl-transfer RNA synthetase (Jo-1)-mediated myositis.
    Arthritis Rheum. 63: 479-87.
  23. Bertilaccio, M.T. et al. (2011) Lack of TIR8/SIGIRR triggers progression of chronic lymphocytic leukemia in mouse models.
    Blood. 118: 660-9.
  24. Zhou, Z. et al. (2011) Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.
    BMC Immunol. 2011; 12:7.
  25. Fanning, S. et al. (2012) Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protection.
    Proc Natl Acad Sci U S A. 109 (6): 2108-13.
  26. Ruf, M.T. et al. (2012) Chemotherapy-Associated Changes of Histopathological Features of Mycobacterium ulcerans Lesions in a Buruli Ulcer Mouse Model.
    Antimicrob Agents Chemother. 56: 687-96.
  27. Carpenter, R.S. et al. (2015) Traumatic spinal cord injury in mice with human immune systems.
    Exp Neurol. 271: 432-44.
  28. Lastrucci, C. et al. (2015) Molecular and cellular profiles of the resolution phase in a damage-associated molecular pattern (DAMP)-mediated peritonitis model and revelation of leukocyte persistence in peritoneal tissues.
    FASEB J. 29 (5): 1914-29.
  29. Gibson-Corley, K.N. et al. (2016) A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis.
    PeerJ. 4: e1600.
  30. Thiele Née Schrewe, L. et al. (2020) Functional relevance of the multi-drug transporter abcg2 on teriflunomide therapy in an animal model of multiple sclerosis.
    J Neuroinflammation. 17 (1): 9.
  31. Allen, A.C. et al. (2021) Parallel in vivo. experimental evolution reveals that increased stress resistance was key for the emergence of persistent tuberculosis bacilli.
    Nat Microbiol. 6 (8): 1082-93.
  32. Chanut, F.J.A. et al. (2021) Conditioning Regimens in Long-Term Pre-Clinical Studies to Support Development of Ex Vivo Gene Therapy: Review of Nonproliferative and Proliferative Changes.
    Hum Gene Ther. 32 (1-2): 66-76.
  33. Jaensch, S.M. et al. (2022) Clinicopathologic and immunophenotypic features in dogs with presumptive large granular lymphocyte leukaemia.
    Aust Vet J. 100 (11): 527-32.
  34. Roca, C.P. et al. (2023) A cross entropy test allows quantitative statistical comparison of t-SNE and UMAP representations
    Cell Reports Methods. 3 (1): 100390.
  35. Kohlmeyer, J.L. et al. (2023) CDK4/6-MEK Inhibition in MPNSTs Causes Plasma Cell Infiltration, Sensitization to PD-L1 Blockade, and Tumor Regression.
    Clin Cancer Res. 29 (17): 3484-97.


Immunohistology - Frozen

Immunohistology - Paraffin

Entrez Gene
GO Terms
GO:0000187 activation of MAPK activity
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
GO:0051726 regulation of cell cycle
GO:0004725 protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007159 leukocyte cell-cell adhesion
GO:0008201 heparin binding
GO:0009897 external side of plasma membrane
GO:0019901 protein kinase binding
GO:0030183 B cell differentiation
GO:0030890 positive regulation of B cell proliferation
GO:0031953 negative regulation of protein autophosphorylation
GO:0034113 heterotypic cell-cell adhesion
GO:0042098 T cell proliferation
GO:0042100 B cell proliferation
GO:0043065 positive regulation of apoptosis
GO:0043395 heparan sulfate proteoglycan binding
GO:0043410 positive regulation of MAPKKK cascade
GO:0045059 positive thymic T cell selection
GO:0045060 negative thymic T cell selection
GO:0045121 membrane raft
GO:0045577 regulation of B cell differentiation
GO:0045588 positive regulation of gamma-delta T cell differentiation
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050855 regulation of B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus



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