CD45 antibody | YW62.3
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|Rat anti Mouse CD45 antibody, clone YW62.3 recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kDa glycoprotein expressed by all leucocytes.
CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains (Komura et al. 1975), this was subsequently expanded to include a third allele encoding Ly5.3 (Shen et al. 1986). Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1 (Morse et al. 1987). Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb (Charbonneau et al. 1988; Zebedee et al. 1991).
A number of different isoforms of CD45 are expressed on murine leucocytes depending on the pattern of alternative splicing of 3 exons termed A, B and C encoding regions of ~ 50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. (Birkeland et al. 1989).
Rat anti mouse CD45 antibody, clone YW62.3 is reactive with all isoforms of murine CD45.
N.B. Some reactivity with human tissue has been observed.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Mouse spleen cells.
- Approx. Protein Concentrations
- Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/mlPack Size: 0.1 mgIgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry||1/100 Pack Size: 0.25 mg, 25 µg
1/50 Pack Size: 0.1 mg
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for CD45 antibody
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Mol Cell Probes. 1 (4): 297-326.
Zirger, J.M. et al. (2012) Immune-mediated loss of transgene expression from virally transduced brain cells is irreversible, mediated by IFNγ, perforin, and TNFα, and due to the elimination of transduced cells.
Mol Ther. 20 (4): 808-19.
Long, G.G. et al. (2010) Hematopoietic Proliferative Lesions in the Spleen of rasH2 Transgenic Mice Treated with MNU.
Toxicol Pathol. 38: 1026-36.
Drake, C. et al. (2011) Brain inflammation is induced by co-morbidities and risk factors for stroke.
Brain Behav Immun. 25: 1113-22.
Chan, D.A. et al (2009) Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment.
Cancer Cell. 15: 527-38.
Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice.
J Neurosci. 30: 9651-8.
Lee, D.C. et al. (2010) LPS- induced inflammation exacerbates phospho-tau pathology in rTg4510 mice.
J Neuroinflammation. 7: 56.
Wang, S. et al. (2008) Drak2 contributes to West Nile virus entry into the brain and lethal encephalitis.
J Immunol. 181: 2084-91.
View The Latest Product References
Paz, H. et al. (2010) The homeobox gene Hhex regulates the earliest stages of definitive hematopoiesis.
Blood. 116: 1254-62.
Reed-Geaghan, E.G. et al. (2010) Deletion of CD14 attenuates Alzheimer's disease pathology by influencing the brain's inflammatory milieu.
J Neurosci. 30: 15369-73.
Yang, R. et al. (2010) Successful treatment of experimental glomerulonephritis with IdeS and EndoS, IgG-degrading streptococcal enzymes.
Nephrol Dial Transplant. 25: 2479-86.
Yang, J. et al. (2010) Evaluation of bone marrow- and brain-derived neural stem cells in therapy of central nervous system autoimmunity.
Am J Pathol. 177: 1989-2001.
Yoshizaki, A. et al. (2010) Cell adhesion molecules regulate fibrotic process via Th1/Th2/Th17 cell balance in a bleomycin-induced scleroderma model.
J Immunol. 185: 2502-15.
Abramowski, D. et al. (2012) Transgenic Expression of Intraneuronal Aβ42 But Not Aβ40 Leads to Cellular Aβ Lesions, Degeneration, and Functional Impairment without Typical Alzheimer's Disease Pathology.
J Neurosci. 32: 1273-83.
Dénes, A. et al. (2010) Chronic systemic infection exacerbates ischemic brain damage via a CCL5 (regulated on activation, normal T-cell expressed and secreted)-mediated proinflammatory response in mice.
J Neurosci. 30: 10086-95.
Kondo, Y. et al. (2007) Osteopetrotic (op/op) mice have reduced microglia, no Abeta deposition, and no changes in dopaminergic neurons.
J Neuroinflammation. 4: 31.
Lee, S. et al. (2010) CX3CR1 deficiency alters microglial activation and reduces beta-amyloid deposition in two Alzheimer's disease mouse models.
Am J Pathol. 177: 2549-62.
Jawhara, S. et al. (2012) Integrin αXβz is a leukocyte receptor for Candida albicans and is essential for protection against fungal infections.
J Immunol. 189 (5): 2468-77.
Yamauchi, S. et al. (2012) Myosin II-dependent exclusion of CD45 from the site of Fcγ receptor activation during phagocytosis.
FEBS Lett. 586: 3229-35.
Yazid, S. et al. (2015) Annexin-A1 restricts Th17 cells and attenuates the severity of autoimmune disease.
J Autoimmun. 58: 1-11.
Kan, M.J. et al. (2015) Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
J Neurosci. 35 (15): 5969-82.
Bachstetter, A.D. et al. (2011) Fractalkine and CX 3 CR1 regulate hippocampal neurogenesis in adult and aged rats.
Neurobiol Aging. 32 (11): 2030-44.
Kuffová, L. et al. (2008) Cross presentation of antigen on MHC class II via the draining lymph node after corneal transplantation in mice.
J Immunol. 180 (3): 1353-61.
Murinello, S. et al. (2014) Fcγ receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration.
Invest Ophthalmol Vis Sci. 55 (1): 247-58.
Mills, J.H. et al. (2012) A2A adenosine receptor signaling in lymphocytes and the central nervous system regulates inflammation during experimental autoimmune encephalomyelitis.
J Immunol. 188 (11): 5713-22.
McMorran, B.J. et al. (2001) G551D CF mice display an abnormal host response and have impaired clearance of Pseudomonas lung disease.
Am J Physiol Lung Cell Mol Physiol. 281 (3): L740-7.
Benson, C. et al. (2015) Voluntary wheel running delays disease onset and reduces pain hypersensitivity in early experimental autoimmune encephalomyelitis (EAE).
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Srivastava, A.K. et al. (2016) Co-transplantation of syngeneic mesenchymal stem cells improves survival of allogeneic glial-restricted precursors in mouse brain.
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Filograna, R. et al. (2021) Mitochondrial dysfunction in adult midbrain dopamine neurons triggers an early immune response.
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J Appl Toxicol. Feb 13 [Epub ahead of print].
- Entrez Gene
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0001915 negative regulation of T cell mediated cytotoxicity
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0001960 negative regulation of cytokine-mediated signaling pathway
- GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
- GO:0051726 regulation of cell cycle
- GO:0004725 protein tyrosine phosphatase activity
- GO:0005887 integral to plasma membrane
- GO:0005925 focal adhesion
- View More GO Terms
- GO:0006469 negative regulation of protein kinase activity
- GO:0006470 protein dephosphorylation
- GO:0007159 leukocyte cell-cell adhesion
- GO:0008201 heparin binding
- GO:0009897 external side of plasma membrane
- GO:0019901 protein kinase binding
- GO:0030183 B cell differentiation
- GO:0030890 positive regulation of B cell proliferation
- GO:0031953 negative regulation of protein autophosphorylation
- GO:0034113 heterotypic cell-cell adhesion
- GO:0042098 T cell proliferation
- GO:0042100 B cell proliferation
- GO:0043065 positive regulation of apoptosis
- GO:0043395 heparan sulfate proteoglycan binding
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045059 positive thymic T cell selection
- GO:0045060 negative thymic T cell selection
- GO:0045121 membrane raft
- GO:0045577 regulation of B cell differentiation
- GO:0045588 positive regulation of gamma-delta T cell differentiation
- GO:0046641 positive regulation of alpha-beta T cell proliferation
- GO:0048304 positive regulation of isotype switching to IgG isotypes
- GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
- GO:0050852 T cell receptor signaling pathway
- GO:0050853 B cell receptor signaling pathway
- GO:0050855 regulation of B cell receptor signaling pathway
- GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
- GO:0051209 release of sequestered calcium ion into cytosol
- GO:0051607 defense response to virus
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