CD45 antibody | IBL-3/16

Rat anti Mouse CD45

Product Type
Monoclonal Antibody
Clone
IBL-3/16
Isotype
IgG1
Specificity
CD45

Product Code Applications Pack Size List Price Your Price Qty
MCA1388
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF IP 0.25 mg loader
List Price Your Price
loader
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Rat anti Mouse CD45 antibody, clone IBL-3/16 recognizes murine Receptor-type tyrosine-protein phosphatase C, also known as CD45, Leukocyte common antigen, T200 or Lymphocyte antigen 5 (Ly5). CD45 is a 1291 amino acid ~175kDa single pass type I transmembrane glycoprotein belonging to the protein -tyrosing phosphatase family. CD45 has two fibronectin type-III domains and two tyrosine protein phosphatase domains (UniProt:: P06800).

Multiple isoforms are generated by alternative splicing with isoforms having differing deletions in the N-terminal region (Saga et al. 1987). Rat anti Mouse CD45 antibody, clone IBL-3/16 is expected to recognize all isoforms of murine CD45.

Rat anti Mouse CD45 antibody, clone IBL-3/16 has been used successfully for the identification of CD45 in murine samples using Immunohistochemical (both cryo and FFPE), immunofluorescence and western blotting techniques (Kondo et al. 2011, Cuadros et al. 2006).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human
Chicken
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Purified B cells from mouse lymph nodes.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from an immunized Wistar rat were fused with cells of the SP2/0-Ag14 mouse myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen 1 1/50 1/100
Immunohistology - Paraffin
Immunohistology - Resin
Immunoprecipitation
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD45 antibody

  1. Balogh, P. et al. (1995) Modulatory effect of CD45 on the MHC Class II-induced homotypic aggregation of B cells in mice.
    In 9th International Congress of Immunology, Abstract book p. 72. No. 422.
  2. Santos, A.M. et al. (2008) Embryonic and postnatal development of microglial cells in the mouse retina.
    J Comp Neurol. 506: 224-39.
  3. Yan, Q. et al. (2003) Anti-inflammatory drug therapy alters beta-amyloid processing and deposition in an animal model of Alzheimer's disease.
    J Neurosci. 23: 7504-9.
  4. Zehntner, S.P. et al. (2007) X-linked inhibitor of apoptosis regulates T cell effector function.
    J Immunol. 2007 Dec 1;179(11):7553-60.
  5. Neutzner, M. et al. (2007) MFG-E8/lactadherin promotes tumor growth in an angiogenesis-dependent transgenic mouse model of multistage carcinogenesis.
    Cancer Res. 67: 6777-85.
  6. Ozmen, L. 145-2C11 (2005) BACE/APPV717F double-transgenic mice develop cerebral amyloidosis and inflammation.
    Neurodegener Dis. 2: 284-98.
  7. Ideguchi, M. et al. (2008) Immune or inflammatory response by the host brain suppresses neuronal differentiation of transplanted ES cell-derived neural precursor cells.
    J Neurosci Res. 86: 1936-43.
  8. Santos, A.M. et al. (2010) Microglial response to light-induced photoreceptor degeneration in the mouse retina.
    J Comp Neurol. 518: 477-92.
  9. View The Latest Product References
  10. McMenamin, P.G. <et al. (2006) Characterisation of rat corneal cells that take up soluble antigen: an in vivo and in vitro study.
    Exp Eye Res. 83: 1268-80.
  11. Clausen, B.H. et al. (2008) Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice.
    J Neuroinflammation. 5: 46.
  12. Laporte, V. et al. (2006) CD40 deficiency mitigates Alzheimer's disease pathology in transgenic mouse models.
    J Neuroinflammation. 3: 3.
  13. Paris, D. et al. (2010) Reduction of beta-amyloid pathology by celastrol in a transgenic mouse model of Alzheimer's disease.
    J Neuroinflammation. 7: 17.
  14. Kondo, Y. et al. (2011) Macrophages counteract demyelination in a mouse model of globoid cell leukodystrophy.
    J Neurosci. 31: 3610-24.
  15. Boger, H.A. et al. (2007) Long-term consequences of methamphetamine exposure in young adults are exacerbated in glial cell line-derived neurotrophic factor heterozygous mice.
    J Neurosci. 27: 8816-25.
  16. Boger, H.A. et al. (2009) Minocycline restores striatal tyrosine hydroxylase in GDNF heterozygous mice but not in methamphetamine-treated mice.
    Neurobiol Dis. 33: 459-66.
  17. Cuadros, M.A. et al. (2006) Specific immunolabeling of brain macrophages and microglial cells in the developing and mature chick central nervous system.
    J Histochem Cytochem. 54: 727-38.
  18. Jiang, H.R. et al. (2001) Total dose and frequency of administration critically affect success of nasal mucosal tolerance induction.
    Br J Ophthalmol. 85: 739-44.
  19. Peng, Y. et al. (2010) L-3-n-butylphthalide improves cognitive impairment and reduces amyloid-beta in a transgenic model of Alzheimer's disease.
    J Neurosci. 30: 8180-9.
  20. Richards, J.G. et al. (2003) PS2APP transgenic mice, coexpressing hPS2mut and hAPPswe, show age-related cognitive deficits associated with discrete brain amyloid deposition and inflammation.
    J Neurosci. 23: 8989-9003.
  21. Li, L. et al. (2008) GRK5 deficiency exaggerates inflammatory changes in TgAPPsw mice.
    J Neuroinflammation. 5: 24.
  22. Rahman, A. et al. (2011) Chronic colitis induces expression of β-defensins in murine intestinal epithelial cells.
    Clin Exp Immunol. 163: 123-30.
  23. Klose, A. et al. (2013) Monocyte/macrophage MMP-14 modulates cell infiltration and T-cell attraction in contact dermatitis but not in murine wound healing.
    Am J Pathol. 182: 755-64.
  24. Ricciardelli, C. et al. (2011) The ADAMTS1 protease gene is required for mammary tumor growth and metastasis.
    Am J Pathol. 179: 3075-85.
  25. Wang, P. et al. (2012) IL-22 signaling contributes to West Nile encephalitis pathogenesis.
    PLoS One. 7: e44153.
  26. Passos, G.F. et al. (2013) The bradykinin B1 receptor regulates Aβ deposition and neuroinflammation in Tg-SwDI mice.
    Am J Pathol. 182: 1740-9.
  27. Medeiros, R. et al. (2011) Loss of muscarinic M1 receptor exacerbates Alzheimer's disease-like pathology and cognitive decline.
    Am J Pathol. 179: 980-91.
  28. Ferrer-Martín, R.M. et al. (2015) Microglial Activation Promotes Cell Survival in Organotypic Cultures of Postnatal Mouse Retinal Explants.
    PLoS One. 10 (8): e0135238.
  29. Tzekov, R. et al. (2016) Sub-Chronic Neuropathological and Biochemical Changes in Mouse Visual System after Repetitive Mild Traumatic Brain Injury.
    PLoS One. 11 (4): e0153608.
  30. Witcher, K.G. et al. (2018) Traumatic brain injury-induced neuronal damage in the somatosensory cortex causes formation of rod-shaped microglia that promote astrogliosis and persistent neuroinflammation.
    Glia. 66 (12): 2719-36.
  31. Boza-Serrano, A. et al. (2019) Galectin-3, a novel endogenous TREM2 ligand, detrimentally regulates inflammatory response in Alzheimer's disease.
    Acta Neuropathol. 138 (2): 251-73.
  32. Da Ros, F. et al. (2017) Targeting Interleukin-1β Protects from Aortic Aneurysms Induced by Disrupted Transforming Growth Factor β Signaling.
    Immunity. 47 (5): 959-973.e9.
  33. Singh, T. et al. (2019) Loss of MafA and MafB expression promotes islet inflammation.
    Sci Rep. 9 (1): 9074.
  34. Tóth, K. et al. (2022) The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.
    PLoS Biol. 20 (1): e3001526.

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Synonyms
LCA
RRID
AB_321729
UniProt
P06800
Entrez Gene
Ptprc
GO Terms
GO:0000187 activation of MAPK activity
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
GO:0051726 regulation of cell cycle
GO:0004725 protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007159 leukocyte cell-cell adhesion
GO:0008201 heparin binding
GO:0009897 external side of plasma membrane
GO:0019901 protein kinase binding
GO:0030183 B cell differentiation
GO:0030890 positive regulation of B cell proliferation
GO:0031953 negative regulation of protein autophosphorylation
GO:0034113 heterotypic cell-cell adhesion
GO:0042098 T cell proliferation
GO:0042100 B cell proliferation
GO:0043065 positive regulation of apoptosis
GO:0043395 heparan sulfate proteoglycan binding
GO:0043410 positive regulation of MAPKKK cascade
GO:0045059 positive thymic T cell selection
GO:0045060 negative thymic T cell selection
GO:0045121 membrane raft
GO:0045577 regulation of B cell differentiation
GO:0045588 positive regulation of gamma-delta T cell differentiation
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050855 regulation of B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus
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