CD44v6 antibody | 9A4

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Rat anti Mouse CD44v6

Product Type
Monoclonal Antibody
Clone
9A4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1967 C E P* WB 0.1 mg
Rat anti Mouse CD44v6, clone 9A4 recognizes an epitope encoded by exon v6 on the variant portion of murine CD44. CD44v6 has been identified along with ICAM-1 as a co-receptor for Met and in mammary epithelia appears to act at the primary Met co-receptor (Di-Cicco et al. 2015).

Rat anti Mouse CD44v6, clone 9A4 has been used successfully for the identification of CD44v6 expressing cells by immunofluorescence (Di-Cicco et al. 2015), western blotting (Lo et al. 2012). immunohistochemistry (Weilenga et al. 1999) along with ELISA and flow cytometry (Weiss et al. 1997).

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
GST-CD44v6 fusion protein.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Fusion Partners
Spleen cells from immunized female DA rats were fused with cells from the SP/0 myeloma.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P15379 Related reagents
Entrez Gene
Cd44 Related reagents
Regulatory
For research purposes only

Applications of CD44v6 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Application Based External Images

Flow Cytometry

Functional Assays

Immunohistology - Paraffin

Product Specific References

References for CD44v6 antibody

  1. Weiss JM et al. (1997) An essential role for CD44 variant isoforms in epidermal Langerhans cell and blood dendritic cell function.
    J Cell Biol. 137 (5): 1137-47.
  2. Wielenga VJ et al. (1999) Expression of CD44 in Apc and Tcf mutant mice implies regulation by the WNT pathway.
    Am J Pathol. 154 (2): 515-23.
  3. Lo, B. et al. (2012) Lkb1 regulates organogenesis and early oncogenesis along AMPK-dependent and -independent pathways.
    J Cell Biol. 199: 1117-30.
  4. Katagiri, Y.U. et al. (1999) CD44 variants but not CD44s cooperate with beta1-containing integrins to permit cells to bind to osteopontin independently of arginine-glycine-aspartic acid, thereby stimulating cell motility and chemotaxis.
    Cancer Res. 59: 219-26.
  5. Hebbard, L. et al. (2000) CD44 expression and regulation during mammary gland development and function.
    J Cell Sci. 113: 2619-30.
  6. Wallach-Dayan, S.B. et al. (2008) DNA vaccination with CD44 variant isoform reduces mammary tumor local growth and lung metastasis.
    Mol Cancer Ther. 7: 1615-23.
  7. Boon, E.M. et al. (2006) Activation of Wnt signaling in the intestinal mucosa of Apc +/min mice does not cause overexpression of the receptor tyrosine kinase Met.
    Cancer Sci. 97: 710-5.
  8. Khaldoyanidi, S. et al. (2002) CD44 variant-specific antibodies trigger hemopoiesis by selective release of cytokines from bone marrow macrophages.
    Blood. 99: 3955-61.
  9. Matrosova, V.Y. et al. (2004) Hyaluronic acid facilitates the recovery of hematopoiesis following 5-fluorouracil administration.
    Stem Cells. 22: 544-55.
  10. Rochman, M. et al. (2000) The CD44 receptor of lymphoma cells: structure-function relationships and mechanism of activation.
    Cell Adhes Commun. 7: 331-47.
  11. Serobyan, N. et al. (2005) Nicotinic acetylcholine receptor-mediated stimulation of endothelial cells results in the arrest of haematopoietic progenitor cells on endothelium.
    Br J Haematol. 129: 257-65.
  12. Termeer, C. et al. (2003) Targeting dendritic cells with CD44 monoclonal antibodies selectively inhibits the proliferation of naive CD4+ T-helper cells by induction of FAS-independent T-cell apoptosis.
    Immunology. 109: 32-40.
  13. Nakagawa, H. et al. (2014) Loss of liver E-cadherin induces sclerosing cholangitis and promotes carcinogenesis.
    Proc Natl Acad Sci U S A. 111(3):1090-5.
  14. Di-Cicco, A. et al. (2015) Paracrine Met signaling triggers epithelial-mesenchymal transition in mammary luminal progenitors, affecting their fate.
    Elife. 2015 Jul 13;4.
  15. Shibata, W. et al. (2017) Helicobacter-induced gastric inflammation alters the properties of gastric tissue stem/progenitor cells.
    BMC Gastroenterol. 17 (1): 145.