CD44 antibody | IM7
Rat anti Mouse CD44
- Product Type
- Monoclonal Antibody
|Rat anti Mouse CD44 antibody, clone IM7 recognizes CD44, also known as H-CAM, HUTCH and lymphocyte antigen 24 (Ly-24).
CD44 is a cell surface receptor for hyaluronic acid, although it can also interact with other ligands such as collagens and matrix metalloproteinases. This protein plays a role in a variety of cellular functions, such as adhesion, lymphocyte activation and lymph node homing. It is also involved in cell migration and plays a role in tumor metastasis. CD44 has many distinct isoforms responsible for its functional diversity.
Rat anti Mouse CD44 antibody, clone IM7 recognizes all isoforms of CD44 and has been reported to inhibit a delayed-type hypersensitivity response in vivo and to induce complement-mediated cytotoxicity.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Human Baboon Cynomolgus monkey Rhesus Monkey Horse Bovine Pig Dog Cat Ferret Mustelid Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Dexamethasone-induced myeloid leukemia M1 cells.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD44 antibody
Lesley, J. et al. (1992) Requirements for hyaluronic acid binding by CD44: a role for the cytoplasmic domain and activation by antibody.
J Exp Med. 175: 257-66.
Camp, R.L. et al. (1993) CD44 is necessary for optimal contact allergic responses but is not required for normal leukocyte extravasation.
J Exp Med. 178: 497-507.
Brocke, S. et al. (1999) Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment.
Proc Natl Acad Sci U S A. 96: 6896-901.
Morrison, H. et al. (2001) The NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth through interactions with CD44.
Genes Dev.15: 968-80.
Mckallip, R.J. et al. (2002) Role of CD44 in activation-induced cell death: CD44-deficient mice exhibit enhanced T cell response to conventional and superantigens.
Int Immunol. 14 (9): 1015-26.
Lesley, J. et al. (2003) Hyaluronan binding by cell surface CD44.
J Biol Chem. 275: 26967-75.
Katoh, S. et al. (2003) A role for CD44 in an antigen-induced murine model of pulmonary eosinophilia.
J Clin Invest. 111: 1563-70.
Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
J Immunol Methods. 336: 71-7.
View The Latest Product References
Ariyoshi, W. et al. (2010) Internalization of aggrecan G1 domain neoepitope ITEGE in chondrocytes requires CD44.
J Biol Chem. 285: 36216-24.
Martín-Villar, E. et al. (2010) Podoplanin associates with CD44 to promote directional cell migration.
Mol Biol Cell. 21: 4387-99.
Hartley, A.N. & Tarleton, R.L. (2015) Chemokine receptor 7 (CCR7)-expression and IFNγ production define vaccine-specific canine T-cell subsets.
Vet Immunol Immunopathol. 164 (3-4): 127-36.
Zhu, C. et al. (2020) Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain.
J Neuroinflammation. 17 (1): 85.
Maeda, S. et al. (2021) NAFLD exacerbates cholangitis and promotes cholangiocellular carcinoma in mice.
Cancer Sci. 112 (4): 1471-80.
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