CD44 antibody | IM7

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Rat anti Mouse CD44:FITC

Rat anti Mouse CD44

Rat anti Mouse CD44:RPE

Product Type
Monoclonal Antibody
Clone
IM7
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4703F F 0.1 mg
MCA4703 C E F FN IP P 0.25 mg
MCA4703PE F 0.1 mg
Rat anti Mouse CD44 antibody, clone IM7 recognizes CD44, also known as H-CAM, HUTCH and lymphocyte antigen 24 (Ly-24).

CD44 is a cell surface receptor for hyaluronic acid, although it can also interact with other ligands such as collagens and matrix metalloproteinases. This protein plays a role in a variety of cellular functions, such as adhesion, lymphocyte activation and lymph node homing. It is also involved in cell migration and plays a role in tumor metastasis. CD44 has many distinct isoforms responsible for its functional diversity.

Rat anti Mouse CD44 antibody, clone IM7 recognizes all isoforms of CD44 and has been reported to inhibit a delayed-type hypersensitivity response in vivo and to induce complement-mediated cytotoxicity.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Baboon
Cynomolgus monkey
Rhesus Monkey
Horse
Bovine
Pig
Dog
Cat
Ferret
Mustelid Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of despatch

More Information

UniProt
P15379 Related reagents
Entrez Gene
Cd44 Related reagents
Regulatory
For research purposes only

Applications of CD44 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20
ELISA
Flow Cytometry 1/20 1/50
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry 1/5 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Application Based External Images

Flow Cytometry

Functional Assays

Immunofluorescence

Western Blotting

Product Specific References

References for CD44 antibody

  1. Camp, R.L. et al. (1993) CD44 is necessary for optimal contact allergic responses but is not required for normal leukocyte extravasation.
    J Exp Med. 178: 497-507.
  2. Brocke, S. et al. (1999) Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment.
    Proc Natl Acad Sci U S A. 96: 6896-901.
  3. Lesley, J. et al. (1992) Requirements for hyaluronic acid binding by CD44: a role for the cytoplasmic domain and activation by antibody.
    J Exp Med. 175: 257-66.
  4. Katoh, S. et al. (2003) A role for CD44 in an antigen-induced murine model of pulmonary eosinophilia.
    J Clin Invest. 111: 1563-70.
  5. Lesley, J. et al. (2003) Hyaluronan binding by cell surface CD44.
    J Biol Chem. 275: 26967-75.
  6. Morrison, H. et al. (2001) The NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth through interactions with CD44.
    Genes Dev.15: 968-80.
  7. Martín-Villar, E. et al. (2010) Podoplanin associates with CD44 to promote directional cell migration.
    Mol Biol Cell. 21: 4387-99.
  8. Ariyoshi, W. et al. (2010) Internalization of aggrecan G1 domain neoepitope ITEGE in chondrocytes requires CD44.
    J Biol Chem. 285: 36216-24.
  9. Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
    J Immunol Methods. 336: 71-7.