CD44 antibody | IM7

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MCA4703
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C E F FN IP P 0.25 mg loader
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Rat anti Mouse CD44 antibody, clone IM7 recognizes CD44, also known as H-CAM, HUTCH and lymphocyte antigen 24 (Ly-24).

CD44 is a cell surface receptor for hyaluronic acid, although it can also interact with other ligands such as collagens and matrix metalloproteinases. This protein plays a role in a variety of cellular functions, such as adhesion, lymphocyte activation and lymph node homing. It is also involved in cell migration and plays a role in tumor metastasis. CD44 has many distinct isoforms responsible for its functional diversity.

Rat anti Mouse CD44 antibody, clone IM7 recognizes all isoforms of CD44 and has been reported to inhibit a delayed-type hypersensitivity response in vivo and to induce complement-mediated cytotoxicity.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Baboon
Cynomolgus monkey
Rhesus Monkey
Horse
Bovine
Pig
Dog
Cat
Ferret
Mustelid Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/20 1/50
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Antigen Retrieval Buffer, pH8.0

References for CD44 antibody

  1. Mckallip, R.J. et al. (2002) Role of CD44 in activation-induced cell death: CD44-deficient mice exhibit enhanced T cell response to conventional and superantigens.
    Int Immunol. 14 (9): 1015-26.
  2. Camp, R.L. et al. (1993) CD44 is necessary for optimal contact allergic responses but is not required for normal leukocyte extravasation.
    J Exp Med. 178: 497-507.
  3. Brocke, S. et al. (1999) Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment.
    Proc Natl Acad Sci U S A. 96: 6896-901.
  4. Lesley, J. et al. (1992) Requirements for hyaluronic acid binding by CD44: a role for the cytoplasmic domain and activation by antibody.
    J Exp Med. 175: 257-66.
  5. Katoh, S. et al. (2003) A role for CD44 in an antigen-induced murine model of pulmonary eosinophilia.
    J Clin Invest. 111: 1563-70.
  6. Lesley, J. et al. (2003) Hyaluronan binding by cell surface CD44.
    J Biol Chem. 275: 26967-75.
  7. Morrison, H. et al. (2001) The NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth through interactions with CD44.
    Genes Dev.15: 968-80.
  8. Martín-Villar, E. et al. (2010) Podoplanin associates with CD44 to promote directional cell migration.
    Mol Biol Cell. 21: 4387-99.
  9. View The Latest Product References
  10. Ariyoshi, W. et al. (2010) Internalization of aggrecan G1 domain neoepitope ITEGE in chondrocytes requires CD44.
    J Biol Chem. 285: 36216-24.
  11. Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
    J Immunol Methods. 336: 71-7.
  12. Maeda, S. et al. (2021) NAFLD exacerbates cholangitis and promotes cholangiocellular carcinoma in mice.
    Cancer Sci. 112 (4): 1471-80.
  13. Hartley, A.N. & Tarleton, R.L. (2015) Chemokine receptor 7 (CCR7)-expression and IFNγ production define vaccine-specific canine T-cell subsets.
    Vet Immunol Immunopathol. 164 (3-4): 127-36.
  14. Zhu, C. et al. (2020) Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain.
    J Neuroinflammation. 17 (1): 85.

Flow Cytometry

Functional Assays

Immunohistology - Paraffin

Western Blotting

Synonyms
H-CAM
PGP-1
RRID
AB_2076194
UniProt
P15379
Entrez Gene
Cd44
View more products with CD44 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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