CD44 antibody | IM7
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Rat anti Mouse CD44
- Product Type
- Monoclonal Antibody
- Clone
- IM7
- Isotype
- IgG2b
- Specificity
- CD44
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| Rat anti Mouse CD44 antibody, clone IM7 recognizes CD44, also known as H-CAM, HUTCH and lymphocyte antigen 24 (Ly-24). CD44 is a cell surface receptor for hyaluronic acid, although it can also interact with other ligands such as collagens and matrix metalloproteinases. This protein plays a role in a variety of cellular functions, such as adhesion, lymphocyte activation and lymph node homing. It is also involved in cell migration and plays a role in tumor metastasis. CD44 has many distinct isoforms responsible for its functional diversity. Rat anti Mouse CD44 antibody, clone IM7 recognizes all isoforms of CD44 and has been reported to inhibit a delayed-type hypersensitivity response in vivo and to induce complement-mediated cytotoxicity. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Baboon Cynomolgus monkey Rhesus Monkey Horse Bovine Pig Dog Cat Ferret Mustelid Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Dexamethasone-induced myeloid leukemia M1 cells.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
1/20 | 1/50 |
| Functional Assays | |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin | |
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| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
References for CD44 antibody
-
Mckallip, R.J. et al. (2002) Role of CD44 in activation-induced cell death: CD44-deficient mice exhibit enhanced T cell response to conventional and superantigens.
Int Immunol. 14 (9): 1015-26. -
Camp, R.L. et al. (1993) CD44 is necessary for optimal contact allergic responses but is not required for normal leukocyte extravasation.
J Exp Med. 178: 497-507. -
Brocke, S. et al. (1999) Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment.
Proc Natl Acad Sci U S A. 96: 6896-901. -
Lesley, J. et al. (1992) Requirements for hyaluronic acid binding by CD44: a role for the cytoplasmic domain and activation by antibody.
J Exp Med. 175: 257-66. -
Katoh, S. et al. (2003) A role for CD44 in an antigen-induced murine model of pulmonary eosinophilia.
J Clin Invest. 111: 1563-70. -
Lesley, J. et al. (2003) Hyaluronan binding by cell surface CD44.
J Biol Chem. 275: 26967-75. -
Morrison, H. et al. (2001) The NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth through interactions with CD44.
Genes Dev.15: 968-80. -
Martín-Villar, E. et al. (2010) Podoplanin associates with CD44 to promote directional cell migration.
Mol Biol Cell. 21: 4387-99.
View The Latest Product References
-
Ariyoshi, W. et al. (2010) Internalization of aggrecan G1 domain neoepitope ITEGE in chondrocytes requires CD44.
J Biol Chem. 285: 36216-24. -
Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
J Immunol Methods. 336: 71-7. -
Maeda, S. et al. (2021) NAFLD exacerbates cholangitis and promotes cholangiocellular carcinoma in mice.
Cancer Sci. 112 (4): 1471-80. -
Hartley, A.N. & Tarleton, R.L. (2015) Chemokine receptor 7 (CCR7)-expression and IFNγ production define vaccine-specific canine T-cell subsets.
Vet Immunol Immunopathol. 164 (3-4): 127-36. -
Zhu, C. et al. (2020) Antinociceptive effect of intrathecal injection of miR-9-5p modified mouse bone marrow mesenchymal stem cells on a mouse model of bone cancer pain.
J Neuroinflammation. 17 (1): 85.
- Synonyms
- H-CAM
- PGP-1
- RRID
- AB_2076194
- UniProt
- P15379
- Entrez Gene
- Cd44
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