CD31 antibody | 2H8
Hamster anti Mouse CD31 antibody, clone N418 (MCA1370Z) used to visualise vascular endothelial cells in mouse kidney (red) by immunofluorescence.
Image caption:
CD248 is expressed by a subset of pericytes resting kidney. A CD248 co-localized with recognized markers of pericytes in resting kidney. Representative images are shown at ×630 magnification of WT resting kidney (right panel) with high-powered images (2 × zoom of ×630 original magnification) on the left panel stained with CD31 (endothelium, red), CD248 (blue) and pericyte markers: NG2, αSMA, PDGFRβ and desmin (green). Scale bar 50 μm. * Indicates an example of cell staining positive for both CD248 (blue) and the pericyte marker (green).
From: Smith SW, Croft AP, Morris HL, et al.
Genetic Deletion of the Stromal Cell Marker CD248 (Endosialin) Protects against the Development of Renal Fibrosis.
Nephron. 2015;131(4):265–77. doi:10.1159/000438754
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Hamster anti Mouse CD31 antibody, clone N418 (MCA1370Z) used to visualise vascular endothelial cells in mouse kidney (red) by immunofluorescence.
Image caption:
Less pericytes are seen in association with endothelium and in the kidney interstitium in CD248-/- mice following UUO. Representative immunostaining for CD31 (red), CD248 (white), NG2 (green) and nuclei blue (A) from sham-operated mice (Aa), WT mice 7 days (Ab) and 14 days (Ac) post UUO ×630 magnification. White arrows indicate pericytes not closely associated with endothelium. WT (Ad) and CD248-/- (Ae) kidney tissue 14 days post UUO (×400 magnification).
From: Smith SW, Croft AP, Morris HL, et al.
Genetic Deletion of the Stromal Cell Marker CD248 (Endosialin) Protects against the Development of Renal Fibrosis.
Nephron. 2015;131(4):265–77. doi:10.1159/000438754
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Hamster anti Mouse CD31:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 2H8
- Isotype
- IgG
- Specificity
- CD31
Antibody Quality and Performance Guarantee
| Hamster anti Mouse CD31 monoclonal antibody, clone 2H8 recognizes murine CD31, also known as Platelet endothelial cell adhesion molecule or PECAM-1. CD31 is a ~130kDa single pass type-1 membrane glycoprotein bearing six C2 Ig-like domains, expressed on all continuous endothelium including arteries, veins and non-sinusoidal capillaries. CD31 is also expressed on all haemopoietic lineages with the exception of the erythroid line (Bogen et al. 1992) . Hamster anti mouse CD31, clone 2H8 effectively inhibits transmigration of activated polymorphonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonitis clone 2H8 blocks acute inflammation (Bogen et al. 1994). CD31 has also been shown to be critically involved in the sensing of changes in shear stress associated with atherosclerotic lesions and in the associated atherogenesis (Stevens et al. 2008). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
Sterile filtered. - Immunogen
- D10.G4.1 cells (Kaye et al. 1984).
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Splenic lymphocytes from an immunized Armenian hamster were fused with cells from the SP2/0 murine myeloma.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20°C only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
0.1ug/ml | |
| Functional Assays | |
||
| Immunofluorescence | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 100μl of whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Hamster (Armenian) IgG Negative Control | ||||||
Source Reference
-
Bogen, S.A. et al. (1992) Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
Am J Pathol. 141 (4): 843-54.
References for CD31 antibody
-
Xie, Y. & Muller, W.A. (1993) Molecular cloning and adhesive properties of murine platelet/endothelial cell adhesion molecule 1.
Proc Natl Acad Sci U S A. 90 (12): 5569-73. -
Bogen, S. et al. (1994) Monoclonal antibody to murine PECAM-1 (CD31) blocks acute inflammation in vivo.
J Exp Med. 179 (3): 1059-64. -
Ishikawa, J. et al. (2002) Use of anti-platelet-endothelial cell adhesion molecule-1 antibody in the control of disease progression in established collagen-induced arthritis in DBA/1J mice.
Jpn J Pharmacol. 88: 332-40. -
Vielhauer, V. et al. (2005) Renal cell-expressed TNF receptor 2, not receptor 1, is essential for the development of glomerulonephritis.
J Clin Invest. 115: 1199-209. -
Thurston, G. et al (2005) Angiopoietin 1 causes vessel enlargement, without angiogenic sprouting, during a critical developmental period
Development. 132: 3317-26. -
Rijcken, E. et al. (2007) PECAM-1 (CD 31) mediates transendothelial leukocyte migration in experimental colitis.
Am J Physiol Gastrointest Liver Physiol. 293: G446-52. -
Wu, Y. et al. (2010) Therapeutic antibody targeting of individual Notch receptors.
Nature. 464: 1052-7. -
Bixel, M.G. et al. (2010) CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis.
Blood. 116: 1172-84.
View The Latest Product References
-
Lonsdorf, A.S. et al. (2012) Engagement of &apha;IIbβ3 (GPIIb/IIIa) with ανβ3 integrin mediates interaction of melanoma cells with platelets: a connection to hematogenous metastasis.
J Biol Chem. 287 (3): 2168-78. -
Brackett, C.M. et al. (2013) IL-17 promotes neutrophil entry into tumor-draining lymph nodes following induction of sterile inflammation.
J Immunol. 191 (8): 4348-57. -
Smith, S.W. et al. (2015) Genetic Deletion of the Stromal Cell Marker CD248 (Endosialin) Protects against the Development of Renal Fibrosis.
Nephron. 131 (4): 265-77. -
Wilhelm, A. et al. (2016) CD248/endosialin critically regulates hepatic stellate cell proliferation during chronic liver injury via a PDGF-regulated mechanism.
Gut. 65 (7): 1175-85.
- Synonyms
- PECAM-1
- RRID
- AB_321653
- UniProt
- Q08481
- Entrez Gene
- Pecam1
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005625 soluble fraction
- GO:0007266 Rho protein signal transduction
- GO:0030054 cell junction
- GO:0030334 regulation of cell migration
- GO:0030485 smooth muscle contractile fiber
- GO:0042060 wound healing
- GO:0045121 membrane raft
- View More GO Terms
View more products with CD31 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up