CD31 antibody | 2H8

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Hamster anti Mouse CD31:Preservative Free

Product Type
Monoclonal Antibody
Clone
2H8
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1370Z F FN IF 0.5 mg
Hamster anti Mouse CD31 monoclonal antibody, clone 2H8 recognizes murine CD31, also known as Platelet endothelial cell adhesion molecule or PECAM-1. CD31 is a ~130kDa single pass type-1 membrane glycoprotein bearing six C2 Ig-like domains, expressed on all continuous endothelium including arteries, veins and non-sinusoidal capillaries. CD31 is also expressed on all haemopoietic lineages with the exception of the erythroid line (Bogen et al. 1992) .

Hamster anti mouse CD31, clone 2H8 effectively inhibits transmigration of activated polymorphonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonitis clone 2H8 blocks acute inflammation (Bogen et al. 1994). CD31 has also been shown to be critically involved in the sensing of changes in shear stress associated with atherosclerotic lesions and in the associated atherogenesis (Stevens et al. 2008).

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by Caprylic Acid Precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.

Sterile filtered.
Immunogen
D10.G4.1 cells (Kaye et al. 1984).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Splenic lymphocytes from an immunized Armenian hamster were fused with cells from the SP2/0 murine myeloma.

Storage Information

Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q08481 Related reagents
Entrez Gene
Pecam1 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005625 soluble fraction
GO:0007266 Rho protein signal transduction
GO:0030054 cell junction
GO:0030334 regulation of cell migration
GO:0030485 smooth muscle contractile fiber
GO:0042060 wound healing
GO:0045121 membrane raft
Regulatory
For research purposes only

Applications of CD31 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 0.1ug/ml
Functional Assays
Immunofluorescence
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 100ul of whole blood.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®549 STAR104D549GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:DyLight®649 STAR104D649GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA 0.1 mg F IF WB
Goat F(ab')2 anti Hamster IgG:FITC STAR104F 0.4 mg F
Goat anti Hamster IgG:Biotin STAR79B 0.8 mg E F
Goat anti Hamster IgG:FITC STAR79F 0.7 mg F
Goat anti Hamster IgG:HRP STAR79P 0.8 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 0.25 mg F

Application Based External Images

Immunofluorescence

Product Specific References

References for CD31 antibody

  1. Bogen, S.A. et al. (1992) Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
    Am J Pathol. 141 (4): 843-54.
  2. Xie, Y. & Muller, W.A. (1993) Molecular cloning and adhesive properties of murine platelet/endothelial cell adhesion molecule 1.
    Proc Natl Acad Sci U S A. 90 (12): 5569-73.
  3. Bogen, S. et al. (1994) Monoclonal antibody to murine PECAM-1 (CD31) blocks acute inflammation in vivo.
    J Exp Med. 179 (3): 1059-64.
  4. Bixel, M.G. et al. (2010) CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis.
    Blood. 116: 1172-84.
  5. Ishikawa, J. et al. (2002) Use of anti-platelet-endothelial cell adhesion molecule-1 antibody in the control of disease progression in established collagen-induced arthritis in DBA/1J mice.
    Jpn J Pharmacol. 88: 332-40.
  6. Thurston, G. et al (2005) Angiopoietin 1 causes vessel enlargement, without angiogenic sprouting, during a critical developmental period
    Development. 132: 3317-26.
  7. Lonsdorf, A.S. et al. (2012) Engagement of αIIbβ3 (GPIIb/IIIa) with ανβ3 Integrin Mediates Interaction of Melanoma Cells with Platelets: A CONNECTION TO HEMATOGENOUS METASTASIS.
    J Biol Chem. 287: 2168-78.
  8. Rijcken, E. et al. (2007) PECAM-1 (CD 31) mediates transendothelial leukocyte migration in experimental colitis.
    Am J Physiol Gastrointest Liver Physiol. 293: G446-52.
  9. Vielhauer, V. et al. (2005) Renal cell-expressed TNF receptor 2, not receptor 1, is essential for the development of glomerulonephritis.
    J Clin Invest. 115: 1199-209.
  10. Brackett, C.M. et al. (2013) IL-17 promotes neutrophil entry into tumor-draining lymph nodes following induction of sterile inflammation.
    J Immunol. 191 (8): 4348-57.
  11. Bixel, M.G. et al. (2010) CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis.
    Blood. 116: 1172-84.
  12. Wu, Y. et al. (2010) Therapeutic antibody targeting of individual Notch receptors.
    Nature. 464: 1052-7.
  13. Wilhelm, A. et al. (2015) CD248/endosialin critically regulates hepatic stellate cell proliferation during chronic liver injury via a PDGF-regulated mechanism.
    Gut. pii: gutjnl-2014-308325.
  14. Smith, S.W. et al. (2015) Genetic Deletion of the Stromal Cell Marker CD248 (Endosialin) Protects against the Development of Renal Fibrosis.
    Nephron. 131 (4): 265-77.