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CD204 antibody | 2F8

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Anti Mouse CD204 Antibody, clone 2F8 thumbnail image 1 Anti Mouse CD204 Antibody, clone 2F8 thumbnail image 2 Anti Mouse CD204 Antibody, clone 2F8 thumbnail image 3 Anti Mouse CD204 Antibody, clone 2F8 thumbnail image 4 Anti Mouse CD204 Antibody, clone 2F8 thumbnail image 5 Anti Mouse CD204 Antibody, clone 2F8 thumbnail image 6 Anti Mouse CD204 Antibody, clone 2F8 thumbnail image 7 Anti Mouse CD204 Antibody, clone 2F8 thumbnail image 8 Anti Mouse CD204 Antibody, clone 2F8 thumbnail image 9
Anti Mouse CD204 Antibody, clone 2F8 gallery image 1
Figure A. PE conjugated Rat anti Mouse CD11b (MCA74PE) and Pacific Blue conjugated Rat IgG2b isotype control (MCA6006PB). Figure B . PE conjugated Rat anti Mouse CD11b (MCA74PE) and Pacific Blue conjugated Rat anti Mouse CD204 (MCA1322PB). All experiments performed on live single J774.2 cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer
Anti Mouse CD204 Antibody, clone 2F8 gallery image 2
Figure A. PE conjugated Rat anti Mouse CD11b (MCA74PE) and Alexa Fluor 700 conjugated Rat IgG2b isotype control (MCA6006A700). Figure B . PE conjugated Rat anti Mouse CD11b (MCA74PE) and Alexa Fluor 700 conjugated Rat anti Mouse CD204 (MCA1322A700). All experiments performed on live single J774.2 cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer
Anti Mouse CD204 Antibody, clone 2F8 gallery image 3
Western Blot staining of J774 lysate (non reduced) probed with Rat anti Mouse CD204 (MCA1322)
Anti Mouse CD204 Antibody, clone 2F8 gallery image 4
Published customer image:
Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) used treat macrophages in vitro prior to assessment of E. coli burden in macrophage cell lysates.
Image caption:
CYP1A1 knockdown macrophages and Rhapontigenin-treated WT macrophages were pre-treated with SR-A antibody (3 μg/ml) for 12 h respectively and then cultured with E.coli for 40 min. Cells were washed and subjected to intracellular bacteria count detection. Group means were compared by Student's t test (n = 6). *p <0.05. NS, no statistical difference

From: Tian LX, Tang X, Zhu JY, Luo L, Ma XY, Cheng SW, Zhang W, Tang WQ, Ma W, Yang X, Lv CZ, Liang HP.
Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis.
Cell Commun Signal. 2020 May 4;18(1):70.
doi: 10.1186/s12964-020-0523-3..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Mouse CD204 Antibody, clone 2F8 gallery image 5
Published customer image:
Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) used to assess SR-A expression in macrophage cultures by western blotting.
Image caption:
SR-A protein expression was quantified by densitometry using GAPDH as the gel-loading control.

From: Tian LX, Tang X, Zhu JY, Luo L, Ma XY, Cheng SW, Zhang W, Tang WQ, Ma W, Yang X, Lv CZ, Liang HP.
Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis.
Cell Commun Signal. 2020 May 4;18(1):70.
doi: 10.1186/s12964-020-0523-3..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Mouse CD204 Antibody, clone 2F8 gallery image 6
Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) followed by horseradish peroxidase Goat anti Rat IgG antibody (STAR72). Low power
Anti Mouse CD204 Antibody, clone 2F8 gallery image 7
Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) followed by horseradish peroxidase Goat anti Rat IgG antibody (STAR72). Medium power
Anti Mouse CD204 Antibody, clone 2F8 gallery image 8
Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) followed by horseradish peroxidase Goat anti Rat IgG antibody (STAR72). High power
Anti Mouse CD204 Antibody, clone 2F8 gallery image 9
Published customer image:
Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) used as a blocking reagent in bacterial phagocytosis experiments with SR-A1 expressing cells.
Image caption:
SR-A1 enhanced L. interrogans adhesion and phagocytosis. Lentivirus transfected HEK293 T vector cells and HEK293 T SR-A1 cells (A), RAW264.7 vector cells and RAW264.7 SR-A1 cells (C) with GFP expression, WT and SR-A1-/- BMDMs (E) or PMs (G) were incubated with L. interrogans strain 56606v at MOI=50 for 1 h in medium without FBS, respectively. Rabbit anti-L. interrogans strain 56606v was used as a specific primary antibody, while Alexa Fluor 647-conjugated or TRITC-conjugated anti-rabbit IgG as a secondary antibody were used before and after permeabilization, respectively. Confocal microscopic images showed leptospires inside (red) or outside (white/yellow) of cells. Positive rates of Leptospira adhesive cells or phagocytic cells were calculated, respectively (B, D, F, H). These data were expressed as the mean ± SEM from at least three experiments. *P<0.05, **P<0.01, ***P<0.001.

From: Wang Y, Fan X, Du L, Liu B, Xiao H, Zhang Y, Wu Y, Liu F, Chang YF, Guo X, He P.
Scavenger receptor A1 participates in uptake of Leptospira interrogans serovar Autumnalis strain 56606v and inflammation in mouse macrophages.
Emerg Microbes Infect. 2021 Apr 30:1-39.
doi: 10.1080/22221751.2021.1925160.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rat anti Mouse CD204:Alexa Fluor® 488

Rat anti Mouse CD204:Alexa Fluor® 647

Rat anti Mouse CD204:Biotin

Rat anti Mouse CD204:FITC

Rat anti Mouse CD204:Low Endotoxin

Rat anti Mouse CD204:Pacific Blue®

Rat anti Mouse CD204

Rat anti Mouse CD204:RPE

Rat anti Mouse CD204:Alexa Fluor® 700

Product Type
Monoclonal Antibody
Clone
2F8
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA1322A488
Datasheet
F
SDS
100 Tests/1ml loader

MCA1322A488T
Datasheet
F
SDS
25 Tests/0.25ml loader

MCA1322A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA1322A647T
Datasheet
F
SDS
25 Tests/0.25ml loader

MCA1322B
Datasheet
F
SDS
0.1 mg loader

MCA1322BT
Datasheet
F
SDS
25 µg loader

MCA1322F
Datasheet
F
SDS
0.1 mg loader

MCA1322FA
Datasheet
F
SDS
50 µg loader

MCA1322FB
Datasheet
F
SDS
0.5 mg loader

MCA1322FT
Datasheet
F
SDS
25 µg loader

MCA1322EL
Datasheet
C* E F FN IP WB*
SDS
0.5 mg loader

MCA1322PB
Datasheet
F
SDS
100 Tests/1ml loader

MCA1322T
Datasheet
C* E F IP WB*
SDS
25 µg loader

MCA1322
Datasheet
C* E F IP WB*
SDS
0.25 mg loader

MCA1322GA
Datasheet
C* E F IF IP WB*
SDS
0.1 mg loader

MCA1322PET
Datasheet
F
SDS
25 Tests/0.25ml loader

MCA1322PE
Datasheet
F
SDS
100 Tests loader

MCA1322A700
Datasheet
F
SDS
100 Tests/1ml loader

Rat anti Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SR-A), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Rat anti Mouse CD204 antibody, clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993).

 Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Rat anti Mouse CD204 antibody, clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Channel catfish
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Pacific Blue - liquid
Product Form
Purified IgG - liquid
Product Form
Pack Size: 25 Tests/0.25ml
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Pack Size: 100 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to Alexa Fluor 700 - liquid
Reconstitution
Pack Size: 25 Tests/0.25ml
Reconstitute with 0.25 ml distilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Raw 264 cell line
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
Pack Size: 0.1 mg, 50 µg, 25 µg
IgG concentration 0.1 mg/ml
Pack Size: 0.5 mg
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
Pack Size: 25 µg, 0.25 mg
IgG concentration 1.0 mg/ml
Pack Size: 0.1 mg
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Pack Size: 25 Tests/0.25ml
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P30204
Entrez Gene
Msr1
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005044 scavenger receptor activity
GO:0006898 receptor-mediated endocytosis
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0010886 positive regulation of cholesterol storage
GO:0030169 low-density lipoprotein particle binding
GO:0030301 cholesterol transport
GO:0034362 low-density lipoprotein particle
GO:0034381 plasma lipoprotein particle clearance
GO:0042953 lipoprotein transport
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD204 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Immunohistology - Paraffin
Immunohistology - Resin
Flow Cytometry 1/10 Neat Pack Size: 0.1 mg, 25 µg
1/50 Pack Size: 0.5 mg
Immunohistology - Paraffin
Immunohistology - Resin
ELISA
Flow Cytometry 1/50 1/100
Functional Assays
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunohistology - Resin
Immunoprecipitation
Western Blotting 2
Flow Cytometry Neat
ELISA
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunohistology - Resin
Immunoprecipitation
Western Blotting 2
Flow Cytometry Neat
Flow Cytometry Neat 1/10
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  2. 2This product recognises CD204 in J774 cells under non-reduced conditions only.
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  2. 2This product recognises CD204 in J774 cells under non-reduced conditions only.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Application Based External Images

Flow Cytometry

Functional Assays

Immunocytochemistry

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for CD204 antibody

  1. Fraser, I. et al. (1993) Divalent cation-independent macrophage adhesion inhibited by monoclonal antibody to murine scavenger receptor.
    Nature. 364 (6435): 343-6.
  2. de Villiers, W.J. et al. (1994) Macrophage-colony-stimulating factor selectively enhances macrophage scavenger receptor expression and function.
    J Exp Med. 180 (2): 705-9.
  3. Hughes, D.A. et al. (1995) Murine macrophage scavenger receptor: in vivo expression and function as receptor for macrophage adhesion in lymphoid and non-lymphoid organs.
    Eur J Immunol. 25 (2): 466-73.
  4. Gordon, S. (1995) The macrophage.
    Bioessays. 17 (11): 977-86.
  5. Hughes, D.A. et al. (1994) Murine M phi scavenger receptor: adhesion function and expression.
    Immunol Lett. 43 (1-2): 7-14.
  6. Aid, S. et al. (2008) Neuroinflammatory response to lipopolysaccharide is exacerbated in mice genetically deficient in cyclooxygenase-2.
    J Neuroinflammation. 5: 17.
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Fluorescent Spectraviewer

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