CD204 antibody | 2F8
Rat anti Mouse CD204:Alexa Fluor® 488
Rat anti Mouse CD204:Alexa Fluor® 647
Rat anti Mouse CD204:Biotin
Rat anti Mouse CD204:FITC
Rat anti Mouse CD204:Low Endotoxin
Rat anti Mouse CD204
Rat anti Mouse CD204:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 2F8
- Isotype
- IgG2b
Rat anti Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SRA), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993).
Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).
Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Channel catfish - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilisedPack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Pack Size: 25 Tests/0.25mlReconstitute with 0.25 ml distilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Raw 264 cell line.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- Pack Size: 25 µg, 50 µg, 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- Pack Size: 25 µg, 0.25 mgIgG concentration 1.0 mg/mlPack Size: 0.1 mgIgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 Tests/0.25mlPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P30204 Related reagents
- Entrez Gene
- Msr1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005044 scavenger receptor activity
- GO:0006898 receptor-mediated endocytosis
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:0010886 positive regulation of cholesterol storage
- GO:0030169 low-density lipoprotein particle binding
- GO:0030301 cholesterol transport
- GO:0034362 low-density lipoprotein particle
- GO:0034381 plasma lipoprotein particle clearance
- GO:0042953 lipoprotein transport
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD204 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | ||
| Immunohistology - Paraffin | |||
| Immunohistology - Resin | |||
| Flow Cytometry | 1/10 | Neat Pack Size: 25 µg, 0.1 mg 1/50 Pack Size: 0.5 mg |
|
| Immunohistology - Paraffin | |||
| Immunohistology - Resin | |||
| ELISA | |||
| Flow Cytometry | 1/50 | 1/100 | |
| Functional Assays | |||
| Immunohistology - Frozen 1 | |||
| Immunohistology - Paraffin | |||
| Immunohistology - Resin | |||
| Immunoprecipitation | |||
| Western Blotting 2 | |||
| ELISA | |||
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen 1 | |||
| Immunohistology - Paraffin | |||
| Immunohistology - Resin | |||
| Immunoprecipitation | |||
| Western Blotting 2 | |||
| Flow Cytometry | Neat |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2This product recognises CD204 in J774 cells under non-reduced conditions only.
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2This product recognises CD204 in J774 cells under non-reduced conditions only.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F |
Application Based External Images
Flow Cytometry
Functional Assays
Immunocytochemistry
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Product Specific References
References for CD204 antibody
-
Fraser, I. et al. (1993) Divalent cation-independent macrophage adhesion inhibited by monoclonal antibody to murine scavenger receptor.
Nature. 364 (6435): 343-6. -
de Villiers, W.J. et al. (1994) Macrophage-colony-stimulating factor selectively enhances macrophage scavenger receptor expression and function.
J Exp Med. 180 (2): 705-9. -
Hughes, D.A. et al. (1995) Murine macrophage scavenger receptor: in vivo expression and function as receptor for macrophage adhesion in lymphoid and non-lymphoid organs.
Eur J Immunol. 25 (2): 466-73. -
Gordon, S. (1995) The macrophage.
Bioessays. 17 (11): 977-86. -
Hughes, D.A. et al. (1994) Murine M phi scavenger receptor: adhesion function and expression.
Immunol Lett. 43 (1-2): 7-14. -
Aid, S. et al. (2008) Neuroinflammatory response to lipopolysaccharide is exacerbated in mice genetically deficient in cyclooxygenase-2.
J Neuroinflammation. 5: 17. -
Daugherty, A. et al. (2000) Polymorphism of class A scavenger receptors in C57BL/6 mice.
J Lipid Res. 41 (10): 1568-77. -
Moldenhauer, L.M. et al. (2010) GM-CSF is an essential regulator of T cell activation competence in uterine dendritic cells during early pregnancy in mice.
J Immunol. 185 (11): 7085-96. -
Luechtenborg, B. et al. (2008) Function of scavenger receptor class A type I/II is not important for smooth muscle foam cell formation.
Eur J Cell Biol. 87: 91-9. -
Sever-Chroneos, Z. et al. (2011) Surfactant Protein A (SP-A)-mediated Clearance of Staphylococcus aureus Involves Binding of SP-A to the Staphylococcal Adhesin Eap and the Macrophage Receptors SP-A Receptor 210 and Scavenger Receptor Class A.
J Biol Chem. 286: 4854-70. -
Yang, C.N. et al. (2011) Mechanism mediating oligomeric Aβ clearance by naïve primary microglia.
Neurobiol Dis. 42 (3): 221-30. -
Hald, A. et al. (2011) MMP9 is protective against lethal inflammatory mass lesions in the mouse colon.
Dis Model Mech. 4: 212-27. -
Swain, S.D. et al. (2011) Pneumocystis infection in an immunocompetent host can promote collateral sensitization to respiratory antigens.
Infect Immun. 79 (5): 1905-14. -
Nikolic, D. et al. (2011) SR-A ligand and M-CSF dynamically regulate SR-A expression and function in primary macrophages via p38 MAPK activation.
BMC Immunol. 12: 37. -
Zaynagetdinov, R et al. (2011) A critical role for macrophages in promotion of urethane-induced lung carcinogenesis.
J Immunol. 187 (11): 5703-11. -
Kaur, H. et al. (2003) Identification of a scavenger receptor homologue on nonspecific cytotoxic cells and evidence for binding to oligodeoxyguanosine.
Fish Shellfish Immunol. 15: 169-81. -
Kaur, H. et al. (2004) Single-base oligodeoxyguanosine-binding proteins on nonspecific cytotoxic cells: identification of a new class of pattern-recognition receptors.
Scand J Immunol. 60: 238-48. -
Koronyo Y et al. (2015) Therapeutic effects of glatiramer acetate and grafted CD115+ monocytes in a mouse model of Alzheimer's disease.
Brain. 138 (Pt 8): 2399-422. -
Nielsen, B.S. et al. (2008) Matrix metalloproteinase 13 is induced in fibroblasts in polyomavirus middle T antigen-driven mammary carcinoma without influencing tumor progression.
PLoS One. 3 (8): e2959. -
Tao, J. et al. (2015) ClC-3 deficiency prevents atherosclerotic lesion development in ApoE-/- mice.
J Mol Cell Cardiol. 87: 237-247. -
Prins, J.R. et al. (2015) Unstable Foxp3+ regulatory T cells and altered dendritic cells are associated with lipopolysaccharide-induced fetal loss in pregnant interleukin 10-deficient mice.
Biol Reprod. 93 (4): 95. -
Almholt, K. et al. (2015) Spontaneous lung and lymph node metastasis in transgenic breast cancer is independent of the urokinase receptor uPAR.
Clin Exp Metastasis. 32 (6): 543-54. -
Verheijden S et al. (2015) Identification of a chronic non-neurodegenerative microglia activation state in a mouse model of peroxisomal β-oxidation deficiency.
Glia. 63 (9): 1606-20. -
Kokubu, Y. et al. (2016) Induction of protumoral CD11chigh macrophages by glioma cancer stem cells through GM-CSF.
Genes Cells. Jan 25. [Epub ahead of print] -
Sapkota, M. et al. (2016) Malondialdehyde-Acetaldehyde-Adducted Surfactant Protein Alters Macrophage Functions Through Scavenger Receptor A.
Alcohol Clin Exp Res. Oct 26. [Epub ahead of print] -
Fujiwara, Y. et al. (2016) Onionin A, a sulfur-containing compound isolated from onions, impairs tumor development and lung metastasis by inhibiting the protumoral and immunosuppressive functions of myeloid cells.
Mol Nutr Food Res. Jul 9. [Epub ahead of print] -
Tsay, H.J. et al. (2016) Identifying N-linked glycan moiety and motifs in the cysteine-rich domain critical for N-glycosylation and intracellular trafficking of SR-AI and MARCO.
J Biomed Sci. 23: 27. -
Horlad, H. et al. (2013) Corosolic acid impairs tumor development and lung metastasis by inhibiting the immunosuppressive activity of myeloid-derived suppressor cells.
Mol Nutr Food Res. 57 (6): 1046-54.
