CD204 antibody | 2F8

Rat anti Mouse CD204

Product Type
Monoclonal Antibody
Clone
2F8
Isotype
IgG2b
Specificity
CD204

Product Code Applications Pack Size List Price Your Price Qty
MCA1322
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E F IP WB * 0.25 mg loader
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loader
MCA1322T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E F IP WB * 25 µg loader
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loader
MCA1322GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * E F IF IP WB * 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SR-A), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Rat anti Mouse CD204 antibody, clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993).

 Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Rat anti Mouse CD204 antibody, clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Channel catfish
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Raw 264 cell line
Approx. Protein Concentrations
Pack Size: 0.25 mg, 25 µg
IgG concentration 1.0 mg/ml
Pack Size: 0.1 mg
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunohistology - Resin
Immunoprecipitation
Western Blotting 2
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  2. 2This product recognises CD204 in J774 cells under non-reduced conditions only.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD204 antibody

  1. Rosen, H. and Hughes, D.A. (1995) Assays of Myeloid Cell Function: Migration and adhesion in vivo.
    Weir Handbook of Experimental Immunology. London, Blackwell Scientific Publications. 5th, ed. In Press.
  2. Allred, K.F. et al. (2006) Estrogen receptor-alpha mediates gender differences in atherosclerosis induced by HIV protease inhibitors.
    J Biol Chem. 281:1419-25.
  3. Anatelli, F. et al. (2006) Macrophage-targeted photosensitizer conjugate delivered by intratumoral injection.
    Mol Pharm. 3: 654-64.
  4. Cainarca, S. et al. (2010) A photoprotein in mouse embryonic stem cells measures Ca2+ mobilization in cells and in animals.
    PLoS One. 5: e8882.
  5. Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.
    PLoS Negl Trop Dis. 4(5):e689.
  6. Fraser, I. et al. (1993) Divalent cation-independent macrophage adhesion inhibited by monoclonal antibody to murine scavenger receptor.
    Nature. 364 (6435): 343-6.
  7. de Villiers, W.J. et al. (1994) Macrophage-colony-stimulating factor selectively enhances macrophage scavenger receptor expression and function.
    J Exp Med. 180 (2): 705-9.
  8. Hughes, D.A. et al. (1995) Murine macrophage scavenger receptor: in vivo expression and function as receptor for macrophage adhesion in lymphoid and non-lymphoid organs.
    Eur J Immunol. 25 (2): 466-73.
  9. View The Latest Product References
  10. Gordon, S. (1995) The macrophage.
    Bioessays. 17 (11): 977-86.
  11. Hughes, D.A. et al. (1994) Murine M phi scavenger receptor: adhesion function and expression.
    Immunol Lett. 43 (1-2): 7-14.
  12. Aid, S. et al. (2008) Neuroinflammatory response to lipopolysaccharide is exacerbated in mice genetically deficient in cyclooxygenase-2.
    J Neuroinflammation. 5: 17.
  13. Daugherty, A. et al. (2000) Polymorphism of class A scavenger receptors in C57BL/6 mice.
    J Lipid Res. 41 (10): 1568-77.
  14. Moldenhauer, L.M. et al. (2010) GM-CSF is an essential regulator of T cell activation competence in uterine dendritic cells during early pregnancy in mice.
    J Immunol. 185 (11): 7085-96.
  15. Luechtenborg, B. et al. (2008) Function of scavenger receptor class A type I/II is not important for smooth muscle foam cell formation.
    Eur J Cell Biol. 87: 91-9.
  16. Sever-Chroneos, Z. et al. (2011) Surfactant Protein A (SP-A)-mediated Clearance of Staphylococcus aureus Involves Binding of SP-A to the Staphylococcal Adhesin Eap and the Macrophage Receptors SP-A Receptor 210 and Scavenger Receptor Class A.
    J Biol Chem. 286: 4854-70.
  17. Yang, C.N. et al. (2011) Mechanism mediating oligomeric Aβ clearance by naïve primary microglia.
    Neurobiol Dis. 42 (3): 221-30.
  18. Hald, A. et al. (2011) MMP9 is protective against lethal inflammatory mass lesions in the mouse colon.
    Dis Model Mech. 4: 212-27.
  19. Swain, S.D. et al. (2011) Pneumocystis infection in an immunocompetent host can promote collateral sensitization to respiratory antigens.
    Infect Immun. 79 (5): 1905-14.
  20. Nikolic, D. et al. (2011) SR-A ligand and M-CSF dynamically regulate SR-A expression and function in primary macrophages via p38 MAPK activation.
    BMC Immunol. 12: 37.
  21. Zaynagetdinov, R et al. (2011) A critical role for macrophages in promotion of urethane-induced lung carcinogenesis.
    J Immunol. 187 (11): 5703-11.
  22. Kaur, H. et al. (2003) Identification of a scavenger receptor homologue on nonspecific cytotoxic cells and evidence for binding to oligodeoxyguanosine.
    Fish Shellfish Immunol. 15: 169-81.
  23. Kaur, H. et al. (2004) Single-base oligodeoxyguanosine-binding proteins on nonspecific cytotoxic cells: identification of a new class of pattern-recognition receptors.
    Scand J Immunol. 60: 238-48.
  24. Koronyo Y et al. (2015) Therapeutic effects of glatiramer acetate and grafted CD115+ monocytes in a mouse model of Alzheimer's disease.
    Brain. 138 (Pt 8): 2399-422.
  25. Nielsen, B.S. et al. (2008) Matrix metalloproteinase 13 is induced in fibroblasts in polyomavirus middle T antigen-driven mammary carcinoma without influencing tumor progression.
    PLoS One. 3 (8): e2959.
  26. Tao, J. et al. (2015) ClC-3 deficiency prevents atherosclerotic lesion development in ApoE-/- mice.
    J Mol Cell Cardiol. 87: 237-247.
  27. Prins, J.R. et al. (2015) Unstable Foxp3+ regulatory T cells and altered dendritic cells are associated with lipopolysaccharide-induced fetal loss in pregnant interleukin 10-deficient mice.
    Biol Reprod. 93 (4): 95.
  28. Almholt, K. et al. (2015) Spontaneous lung and lymph node metastasis in transgenic breast cancer is independent of the urokinase receptor uPAR.
    Clin Exp Metastasis. 32 (6): 543-54.
  29. Verheijden S et al. (2015) Identification of a chronic non-neurodegenerative microglia activation state in a mouse model of peroxisomal β-oxidation deficiency.
    Glia. 63 (9): 1606-20.
  30. Kokubu, Y. et al. (2016) Induction of protumoral CD11c(high) macrophages by glioma cancer stem cells through GM-CSF.
    Genes Cells. 21 (3): 241-51.
  31. Sapkota, M. et al. (2016) Malondialdehyde-Acetaldehyde-Adducted Surfactant Protein Alters Macrophage Functions Through Scavenger Receptor A.
    Alcohol Clin Exp Res. 40 (12): 2563-2572.
  32. Fujiwara, Y. et al. (2016) Onionin A, a sulfur-containing compound isolated from onions, impairs tumor development and lung metastasis by inhibiting the protumoral and immunosuppressive functions of myeloid cells.
    Mol Nutr Food Res. Jul 9. [Epub ahead of print]
  33. Tsay, H.J. et al. (2016) Identifying N-linked glycan moiety and motifs in the cysteine-rich domain critical for N-glycosylation and intracellular trafficking of SR-AI and MARCO.
    J Biomed Sci. 23: 27.
  34. Horlad, H. et al. (2013) Corosolic acid impairs tumor development and lung metastasis by inhibiting the immunosuppressive activity of myeloid-derived suppressor cells.
    Mol Nutr Food Res. 57 (6): 1046-54.
  35. Shiau, D.J. et al. (2020) Hepatocellular carcinoma-derived high mobility group box 1 triggers M2 macrophage polarization via a TLR2/NOX2/autophagy axis.
    Sci Rep. 10 (1): 13582.
  36. Tian, L.X. et al. (2020) Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis.
    Cell Commun Signal. 18 (1): 70.
  37. Wang, Y. et al. (2021) Scavenger receptor A1 participates in uptake of Leptospira interrogans serovar Autumnalis strain 56606v and inflammation in mouse macrophages.
    Emerg Microbes Infect. : 1-39.
  38. Haupt, F. et al. (2019) Retinal myeloid cells regulate tip cell selection and vascular branching morphogenesis via Notch ligand Delta-like 1.
    Sci Rep. 9 (1): 9798.
  39. Mañucat-Tan, N. et al. (2021) Hypochlorite-induced aggregation of fibrinogen underlies a novel antioxidant role in blood plasma.
    Redox Biol. 40: 101847.
  40. Yamamoto, M. et al. (2019) Evaluation of neurobehavioral impairment in methylmercury-treated KK-Ay mice by dynamic weight-bearing test.
    J Appl Toxicol. 39 (2): 221-30.

Blocking assay

Flow Cytometry

Functional Assays

Immunocytochemistry

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Synonyms
Scavenger Receptor Type I/II
RRID
AB_322413
UniProt
P30204
Entrez Gene
Msr1
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005044 scavenger receptor activity
GO:0006898 receptor-mediated endocytosis
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0010886 positive regulation of cholesterol storage
GO:0030169 low-density lipoprotein particle binding
GO:0030301 cholesterol transport
GO:0034362 low-density lipoprotein particle
GO:0034381 plasma lipoprotein particle clearance
GO:0042953 lipoprotein transport
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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