CD204 antibody | 2F8
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Rat anti Mouse CD204
- Product Type
- Monoclonal Antibody
|Product Code||Applications||Pack Size||List Price||Your Price||Qty|
|Rat anti Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SR-A), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Rat anti Mouse CD204 antibody, clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993).
Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Rat anti Mouse CD204 antibody, clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Pig Channel catfish
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Raw 264 cell line
- Approx. Protein Concentrations
- Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/mlPack Size: 0.1 mgIgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1|
|Immunohistology - Paraffin|
|Immunohistology - Resin|
|Western Blotting 2|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2This product recognises CD204 in J774 cells under non-reduced conditions only.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD204 antibody
Fraser, I. et al. (1993) Divalent cation-independent macrophage adhesion inhibited by monoclonal antibody to murine scavenger receptor.
Nature. 364 (6435): 343-6.
de Villiers, W.J. et al. (1994) Macrophage-colony-stimulating factor selectively enhances macrophage scavenger receptor expression and function.
J Exp Med. 180 (2): 705-9.
Hughes, D.A. et al. (1994) Murine M phi scavenger receptor: adhesion function and expression.
Immunol Lett. 43 (1-2): 7-14.
Hughes, D.A. et al. (1995) Murine macrophage scavenger receptor: in vivo expression and function as receptor for macrophage adhesion in lymphoid and non-lymphoid organs.
Eur J Immunol. 25 (2): 466-73.
Daugherty, A. et al. (2000) Polymorphism of class A scavenger receptors in C57BL/6 mice.
J Lipid Res. 41 (10): 1568-77.
Kaur, H. et al. (2003) Identification of a scavenger receptor homologue on nonspecific cytotoxic cells and evidence for binding to oligodeoxyguanosine.
Fish Shellfish Immunol. 15: 169-81.
Kaur, H. et al. (2004) Single-base oligodeoxyguanosine-binding proteins on nonspecific cytotoxic cells: identification of a new class of pattern-recognition receptors.
Scand J Immunol. 60: 238-48.
Allred, K.F. et al. (2006) Estrogen receptor-alpha mediates gender differences in atherosclerosis induced by HIV protease inhibitors.
J Biol Chem. 281:1419-25.
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Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.
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Hald, A. et al. (2011) MMP9 is protective against lethal inflammatory mass lesions in the mouse colon.
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Nikolic, D. et al. (2011) SR-A ligand and M-CSF dynamically regulate SR-A expression and function in primary macrophages via p38 MAPK activation.
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Sever-Chroneos, Z. et al. (2011) Surfactant Protein A (SP-A)-mediated Clearance of Staphylococcus aureus Involves Binding of SP-A to the Staphylococcal Adhesin Eap and the Macrophage Receptors SP-A Receptor 210 and Scavenger Receptor Class A.
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- Scavenger Receptor Type I/II
- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005044 scavenger receptor activity
- GO:0006898 receptor-mediated endocytosis
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:0010886 positive regulation of cholesterol storage
- GO:0030169 low-density lipoprotein particle binding
- GO:0030301 cholesterol transport
- GO:0034362 low-density lipoprotein particle
- View More GO Terms
- GO:0034381 plasma lipoprotein particle clearance
- GO:0042953 lipoprotein transport
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