CD204 antibody | 2F8
Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) used treat macrophages in vitro prior to assessment of E. coli burden in macrophage cell lysates.
Image caption:
CYP1A1 knockdown macrophages and Rhapontigenin-treated WT macrophages were pre-treated with SR-A antibody (3 μg/ml) for 12 h respectively and then cultured with E.coli for 40 min. Cells were washed and subjected to intracellular bacteria count detection. Group means were compared by Student's t test (n = 6). *p <0.05. NS, no statistical difference
From: Tian LX, Tang X, Zhu JY, Luo L, Ma XY, Cheng SW, Zhang W, Tang WQ, Ma W, Yang X, Lv CZ, Liang HP.
Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis.
Cell Commun Signal. 2020 May 4;18(1):70.
doi: 10.1186/s12964-020-0523-3..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) used to assess SR-A expression in macrophage cultures by western blotting.
Image caption:
SR-A protein expression was quantified by densitometry using GAPDH as the gel-loading control.
From: Tian LX, Tang X, Zhu JY, Luo L, Ma XY, Cheng SW, Zhang W, Tang WQ, Ma W, Yang X, Lv CZ, Liang HP.
Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis.
Cell Commun Signal. 2020 May 4;18(1):70.
doi: 10.1186/s12964-020-0523-3..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) used as a blocking reagent in bacterial phagocytosis experiments with SR-A1 expressing cells.
Image caption:
SR-A1 enhanced L. interrogans adhesion and phagocytosis. Lentivirus transfected HEK293 T vector cells and HEK293 T SR-A1 cells (A), RAW264.7 vector cells and RAW264.7 SR-A1 cells (C) with GFP expression, WT and SR-A1-/- BMDMs (E) or PMs (G) were incubated with L. interrogans strain 56606v at MOI=50 for 1 h in medium without FBS, respectively. Rabbit anti-L. interrogans strain 56606v was used as a specific primary antibody, while Alexa Fluor 647-conjugated or TRITC-conjugated anti-rabbit IgG as a secondary antibody were used before and after permeabilization, respectively. Confocal microscopic images showed leptospires inside (red) or outside (white/yellow) of cells. Positive rates of Leptospira adhesive cells or phagocytic cells were calculated, respectively (B, D, F, H). These data were expressed as the mean ± SEM from at least three experiments. *P<0.05, **P<0.01, ***P<0.001.
From: Wang Y, Fan X, Du L, Liu B, Xiao H, Zhang Y, Wu Y, Liu F, Chang YF, Guo X, He P.
Scavenger receptor A1 participates in uptake of Leptospira interrogans serovar Autumnalis strain 56606v and inflammation in mouse macrophages.
Emerg Microbes Infect. 2021 Apr 30:1-39.
doi: 10.1080/22221751.2021.1925160.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) used to evaluate SR-A expression in macrophages following exposure to M-CSF by western blotting.
Image caption:
M-CSF induces SR-A expression and AcLDL association.
(A) Cultured resident MPMs were incubated with the indicated concentrations of M-CSF for 24 hrs. Subsequently, SR-A and GAPDH proteins were detected in lysates prepared from treated cells by immunoblotting with specific antibodies. A representative immunoblot from a single experiment and the results (mean ± SEM) from at least three separate experiments are shown.
From: Nikolic D, Calderon L, Du L, Post SR.
SR-A ligand and M-CSF dynamically regulate SR-A expression and function in primary macrophages via p38 MAPK activation.
BMC Immunol. 2011 Jul 7;12:37.
doi: 10.1186/1471-2172-12-37.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) used to evaluate SR-A expression in macrophages following exposure to M-CSF by western blotting.
Image caption:
M-CSF induces SR-A expression and AcLDL association.
(A) Cultured resident MPMs were incubated with the indicated concentrations of M-CSF for 24 hrs. Subsequently, SR-A and GAPDH proteins were detected in lysates prepared from treated cells by immunoblotting with specific antibodies. A representative immunoblot from a single experiment and the results (mean ± SEM) from at least three separate experiments are shown.
From: Nikolic D, Calderon L, Du L, Post SR.
SR-A ligand and M-CSF dynamically regulate SR-A expression and function in primary macrophages via p38 MAPK activation.
BMC Immunol. 2011 Jul 7;12:37.
doi: 10.1186/1471-2172-12-37.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) used to evaluate SR-A expression in macrophages following exposure to AcLDL by western blotting.
Image caption:
AcLDL reversibly stimulates SR-A expression via activation of p38 MAPK.
(A) Cultured resident MPM were pretreated, as indicated, with specific inhibitors of p38 [SB203580 (10 μM)], JNK [SP600125 (20 μM)] or ERK1/2 [PD98059 (10 μM)] for 20 minutes at 37°C. Cells were then treated with or without AcLDL (50 μg/ml) for 24 hrs at 37°C, and as indicated incubated without AcLDL for additional 72 hrs (Reverse). Cell lysates were prepared and SR-A protein expression quantified by immunoblotting. A representative immunoblot from a single experiment and the results (mean ± SEM) from at least three separate experiments are shown. * denotes significant difference (p <0.05) from AcLDL treated control value (ANOVA with Dunnett's multiple comparison).
From: Nikolic D, Calderon L, Du L, Post SR.
SR-A ligand and M-CSF dynamically regulate SR-A expression and function in primary macrophages via p38 MAPK activation.
BMC Immunol. 2011 Jul 7;12:37.
doi: 10.1186/1471-2172-12-37.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) used to detect SR-A in a range of mouse strains by immunocytochemistry.
Image caption:
2F8 did not immunostain MPM from C57BL/6 mice. MPM were harvested and maintained in cell culture for 48 h from Swiss (A and E), BALB/c (B and F), DBA/2 (C and G), and C57BL/6 (D and H). Immunoreactive cells were visualized with red chromagen, and nuclei were counterstained with hematoxylin. Immunostaining using a rabbit antiserum to mouse macrophages indicated that all adherent cells were macrophages (A–D). The detection of SR-A using monoclonal rat antimouse 2F8 was present in Swiss, BALB/c, and DBA/2 (E–G) but absent in C57BL/6 cells (H). Cells were photographed at ×400 original magnification.
From: Daugherty A, Whitman SC, Block AE, Rateri DL.
Polymorphism of class A scavenger receptors in C57BL/6 mice.
J Lipid Res. 2000 Oct;41(10):1568-77.
doi: 10.1016/S0022-2275(20)31989-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F WB FN C BA IC ResetRat anti Mouse CD204
- Product Type
- Monoclonal Antibody
- Clone
- 2F8
- Isotype
- IgG2b
- Specificity
- CD204
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SR-A), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Rat anti Mouse CD204 antibody, clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993). Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Rat anti Mouse CD204 antibody, clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000). |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Channel catfish - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Raw 264 cell line
- Approx. Protein Concentrations
- Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/mlPack Size: 0.1 mgIgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
1/50 | 1/100 |
| Immunofluorescence | |
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| Immunohistology - Frozen 1 | |
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| Immunohistology - Paraffin | |||
| Immunohistology - Resin | |||
| Immunoprecipitation | |
||
| Western Blotting 2 | |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2This product recognises CD204 in J774 cells under non-reduced conditions only.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD204 antibody
-
Fraser, I. et al. (1993) Divalent cation-independent macrophage adhesion inhibited by monoclonal antibody to murine scavenger receptor.
Nature. 364 (6435): 343-6. -
de Villiers, W.J. et al. (1994) Macrophage-colony-stimulating factor selectively enhances macrophage scavenger receptor expression and function.
J Exp Med. 180 (2): 705-9. -
Hughes, D.A. et al. (1994) Murine M phi scavenger receptor: adhesion function and expression.
Immunol Lett. 43 (1-2): 7-14. -
Hughes, D.A. et al. (1995) Murine macrophage scavenger receptor: in vivo expression and function as receptor for macrophage adhesion in lymphoid and non-lymphoid organs.
Eur J Immunol. 25 (2): 466-73. -
Daugherty, A. et al. (2000) Polymorphism of class A scavenger receptors in C57BL/6 mice.
J Lipid Res. 41 (10): 1568-77. -
Kaur, H. et al. (2003) Identification of a scavenger receptor homologue on nonspecific cytotoxic cells and evidence for binding to oligodeoxyguanosine.
Fish Shellfish Immunol. 15: 169-81. -
Kaur, H. et al. (2004) Single-base oligodeoxyguanosine-binding proteins on nonspecific cytotoxic cells: identification of a new class of pattern-recognition receptors.
Scand J Immunol. 60: 238-48. -
Allred, K.F. et al. (2006) Estrogen receptor-alpha mediates gender differences in atherosclerosis induced by HIV protease inhibitors.
J Biol Chem. 281:1419-25.
View The Latest Product References
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Anatelli, F. et al. (2006) Macrophage-targeted photosensitizer conjugate delivered by intratumoral injection.
Mol Pharm. 3: 654-64. -
Luechtenborg, B. et al. (2008) Function of scavenger receptor class A type I/II is not important for smooth muscle foam cell formation.
Eur J Cell Biol. 87: 91-9. -
Nielsen, B.S. et al. (2008) Matrix metalloproteinase 13 is induced in fibroblasts in polyomavirus middle T antigen-driven mammary carcinoma without influencing tumor progression.
PLoS One. 3 (8): e2959. -
Aid, S. et al. (2008) Neuroinflammatory response to lipopolysaccharide is exacerbated in mice genetically deficient in cyclooxygenase-2.
J Neuroinflammation. 5: 17. -
Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.
PLoS Negl Trop Dis. 4(5):e689. -
Moldenhauer, L.M. et al. (2010) GM-CSF is an essential regulator of T cell activation competence in uterine dendritic cells during early pregnancy in mice.
J Immunol. 185 (11): 7085-96. -
Cainarca, S. et al. (2010) A photoprotein in mouse embryonic stem cells measures Ca2+ mobilization in cells and in animals.
PLoS One. 5: e8882. -
Hald, A. et al. (2011) MMP9 is protective against lethal inflammatory mass lesions in the mouse colon.
Dis Model Mech. 4: 212-27. -
Nikolic, D. et al. (2011) SR-A ligand and M-CSF dynamically regulate SR-A expression and function in primary macrophages via p38 MAPK activation.
BMC Immunol. 12: 37. -
Zaynagetdinov, R et al. (2011) A critical role for macrophages in promotion of urethane-induced lung carcinogenesis.
J Immunol. 187 (11): 5703-11. -
Yang, C.N. et al. (2011) Mechanism mediating oligomeric Aβ clearance by naïve primary microglia.
Neurobiol Dis. 42 (3): 221-30. -
Sever-Chroneos, Z. et al. (2011) Surfactant Protein A (SP-A)-mediated Clearance of Staphylococcus aureus Involves Binding of SP-A to the Staphylococcal Adhesin Eap and the Macrophage Receptors SP-A Receptor 210 and Scavenger Receptor Class A.
J Biol Chem. 286: 4854-70. -
Swain, S.D. et al. (2011) Pneumocystis infection in an immunocompetent host can promote collateral sensitization to respiratory antigens.
Infect Immun. 79 (5): 1905-14. -
Horlad, H. et al. (2013) Corosolic acid impairs tumor development and lung metastasis by inhibiting the immunosuppressive activity of myeloid-derived suppressor cells.
Mol Nutr Food Res. 57 (6): 1046-54. -
Verheijden S et al. (2015) Identification of a chronic non-neurodegenerative microglia activation state in a mouse model of peroxisomal β-oxidation deficiency.
Glia. 63 (9): 1606-20. -
Almholt, K. et al. (2015) Spontaneous lung and lymph node metastasis in transgenic breast cancer is independent of the urokinase receptor uPAR.
Clin Exp Metastasis. 32 (6): 543-54. -
Tao, J. et al. (2015) ClC-3 deficiency prevents atherosclerotic lesion development in ApoE-/- mice.
J Mol Cell Cardiol. 87: 237-247. -
Koronyo Y et al. (2015) Therapeutic effects of glatiramer acetate and grafted CD115+ monocytes in a mouse model of Alzheimer's disease.
Brain. 138 (Pt 8): 2399-422. -
Prins, J.R. et al. (2015) Unstable Foxp3+ regulatory T cells and altered dendritic cells are associated with lipopolysaccharide-induced fetal loss in pregnant interleukin 10-deficient mice.
Biol Reprod. 93 (4): 95. -
Kokubu, Y. et al. (2016) Induction of protumoral CD11c(high) macrophages by glioma cancer stem cells through GM-CSF.
Genes Cells. 21 (3): 241-51. -
Fujiwara, Y. et al. (2016) Onionin A, a sulfur-containing compound isolated from onions, impairs tumor development and lung metastasis by inhibiting the protumoral and immunosuppressive functions of myeloid cells.
Mol Nutr Food Res. 60 (11): 2467-80. -
Sapkota, M. et al. (2016) Malondialdehyde-Acetaldehyde-Adducted Surfactant Protein Alters Macrophage Functions Through Scavenger Receptor A.
Alcohol Clin Exp Res. 40 (12): 2563-2572. -
Tsay, H.J. et al. (2016) Identifying N-linked glycan moiety and motifs in the cysteine-rich domain critical for N-glycosylation and intracellular trafficking of SR-AI and MARCO.
J Biomed Sci. 23: 27. -
Haupt, F. et al. (2019) Retinal myeloid cells regulate tip cell selection and vascular branching morphogenesis via Notch ligand Delta-like 1.
Sci Rep. 9 (1): 9798. -
Yamamoto, M. et al. (2019) Evaluation of neurobehavioral impairment in methylmercury-treated KK-Ay mice by dynamic weight-bearing test.
J Appl Toxicol. 39 (2): 221-30. -
Shiau, D.J. et al. (2020) Hepatocellular carcinoma-derived high mobility group box 1 triggers M2 macrophage polarization via a TLR2/NOX2/autophagy axis.
Sci Rep. 10 (1): 13582. -
Tian, L.X. et al. (2020) Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis.
Cell Commun Signal. 18 (1): 70. -
Li, S. et al. (2020) Activated Bone Marrow-Derived Macrophages Eradicate Alzheimer's-Related Aβ(42) Oligomers and Protect Synapses.
Front Immunol. 11: 49. -
Wang, Y. et al. (2021) Scavenger receptor A1 participates in uptake of Leptospira interrogans serovar Autumnalis strain 56606v and inflammation in mouse macrophages.
Emerg Microbes Infect. : 1-39. -
Mañucat-Tan, N. et al. (2021) Hypochlorite-induced aggregation of fibrinogen underlies a novel antioxidant role in blood plasma.
Redox Biol. 40: 101847. -
Jia, J. et al. (2022) Ferritin triggers neutrophil extracellular trap-mediated cytokine storm through Msr1 contributing to adult-onset Still's disease pathogenesis.
Nat Commun. 13 (1): 6804.
Further Reading
-
Gordon, S. (1995) The macrophage.
Bioessays. 17 (11): 977-86.
- Synonyms
- Scavenger Receptor Type I/II
- RRID
- AB_322413
- UniProt
- P30204
- Entrez Gene
- Msr1
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005044 scavenger receptor activity
- GO:0006898 receptor-mediated endocytosis
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:0010886 positive regulation of cholesterol storage
- GO:0030169 low-density lipoprotein particle binding
- GO:0030301 cholesterol transport
- GO:0034362 low-density lipoprotein particle
- View More GO Terms
- GO:0034381 plasma lipoprotein particle clearance
- GO:0042953 lipoprotein transport
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