CD169 antibody | MOMA-1

Rat anti Mouse CD169

Product Type
Monoclonal Antibody
Clone
MOMA-1
Isotype
IgG2a
Specificity
CD169

Product Code Applications Pack Size List Price Your Price Qty
MCA947G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IF 0.25 mg loader
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loader
MCA947GA
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SDS Safety Datasheet SDS
C IF 0.1 mg loader
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loader
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Rat anti Mouse CD169, clone MOMA-1 recognizes murine CD169, also known as sialoadhesin or Siglec-1. CD169 is a lectin-like receptor expressed by certain populations of macrophages including marginal zone metallophils of the spleen, subcapsular macrophages of lymph nodes and stromal macrophages in bone marrow (Morris et al. 1991).

CD169 is a ~185 kDa sialic acid binding receptor containing 17 immunoglobulin-like domains (Crocker et al. 1992). Expression of CD169 can be induced on macrophages in culture by a serum factor and further modulated by cytokine exposure (McWilliam et al. 1992).

Rat anti mouse CD169, clone MOMA-1 has been used for the in vivo depletion of specific macrophage populations (Kraal et al. 1988).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Stromal (reticular) elements from mouse lymph nodes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from hyperimmunized mice were fused with cells from the murine SP2/0 myeloma.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Lymphoid tissue

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for CD169 antibody

  1. Caton, M.L. et al. (2007) Notch-RBP-J signaling controls the homeostasis of CD8- dendritic cells in the spleen.
    J Exp Med. 204 (7): 1653-64.
  2. Alcamo, E. et al. (2002) Requirement for the NF-κB family member RelA in the development of secondary lymphoid organs.
    J Exp Med. 195: 233-44.
  3. Cadman, E.T. et al. (2008) Alterations of splenic architecture in malaria are induced independently of Toll-like receptors 2, 4, and 9 or MyD88 and may affect antibody affinity.
    Infect Immun. 76: 3924-31.
  4. Kaisho, T. et al. (2001) IkappaB kinase alpha is essential for mature B cell development and function.
    J Exp Med. 193: 417-26.
  5. Miosge, L.A. et al. (2002) Analysis of an ethylnitrosourea-generated mouse mutation defines a cell intrinsic role of nuclear factor kappaB2 in regulating circulating B cell numbers.
    J Exp Med.196: 1113-9.
  6. Zhou, Z. et al. (2011) Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.
    BMC Immunol. 12:7.
  7. Muppidi, J.R. et al. (2011) Cannabinoid receptor 2 positions and retains marginal zone B cells within the splenic marginal zone.
    J Exp Med. 208 (10): 1941-8.
  8. Benlagha, K. et al. (2004) Mechanisms governing B cell developmental defects in invariant chain-deficient mice.
    J Immunol. 172: 2076-83.
  9. View The Latest Product References
  10. Cariappa, A. et al. (2005) The CD9 tetraspanin is not required for the development of peripheral B cells or for humoral immunity.
    J Immunol. 175: 2925-30.
  11. Gangadharan, B. et al. (2008) Murine gamma herpesvirus-induced fibrosis is associated with the development of alternatively activated macrophages.
    J Leukoc Biol. 84: 50-8.
  12. Rolf, J. et al. (2005) The enlarged population of marginal zone/CD1d(high) B lymphocytes in nonobese diabetic mice maps to diabetes susceptibility region Idd11.
    J Immunol. 174: 4821-7.
  13. Kraal, G. and Janse, M. (1986) Marginal metallophilic cells of the mouse spleen identified by a monoclonal antibody.
    Immunology. 58: 665-9.
  14. Oetke, C. et al. (2006) The antigen recognized by MOMA-I is sialoadhesin.
    Immunol Lett. 106: 96-98.
  15. Tumanov, A.V. et al. (2010) Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs.
    Blood. 116 (18): 3456-64.
  16. Karlsson, M.C. et al. (2003) Macrophages control the retention and trafficking of B lymphocytes in the splenic marginal zone.
    J Exp Med. 198: 333-40.
  17. Kanayama, N. et al. (2005) Analysis of marginal zone B cell development in the mouse with limited B cell diversity: role of the antigen receptor signals in the recruitment of B cells to the marginal zone.
    J Immunol. 174 (3): 1438-45.
  18. Höpken, U.E. et al. (2004) Distinct and overlapping roles of CXCR5 and CCR7 in B-1 cell homing and early immunity against bacterial pathogens.
    J Leukoc Biol. 76 (3): 709-18.
  19. Ferguson, A.R. et al. (2004) Marginal zone B cells transport and deposit IgM-containing immune complexes onto follicular dendritic cells.
    Int Immunol. 16 (10): 1411-22.
  20. Girkontaite, I. et al. (2004) The sphingosine-1-phosphate (S1P) lysophospholipid receptor S1P3 regulates MAdCAM-1+ endothelial cells in splenic marginal sinus organization.
    J Exp Med. 200 (11): 1491-501.
  21. Acevedo-Suárez, C.A. et al. (2005) Uncoupling of anergy from developmental arrest in anti-insulin B cells supports the development of autoimmune diabetes.
    J Immunol. 174 (2): 827-33.
  22. Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
    J Immunol. 186: 3441-51.
  23. Bhattacharyya, S. et al. (2011) NFATc1 affects mouse splenic B cell function by controlling the calcineurin-NFAT signaling network.
    J Exp Med. 208 (4): 823-39.
  24. Jang, I.K. et al. (2011) Growth-factor receptor-bound protein-2 (Grb2) signaling in B cells controls lymphoid follicle organization and germinal center reaction.
    Proc Natl Acad Sci U S A. 108: 7926-31.
  25. Rehm, A. et al. (2011) Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid organs.
    Blood. 118 (4): 1020-33.
  26. Mattsson, J. et al. (2011) Complement activation and complement receptors on follicular dendritic cells are critical for the function of a targeted adjuvant.
    J Immunol. 187: 3641-52.
  27. Whipple, E.C. et al. (2004) Analyses of the in vivo trafficking of stoichiometric doses of an anti-complement receptor 1/2 monoclonal antibody infused intravenously in mice.
    J Immunol. 173 (4): 2297-306.
  28. Zhang, Z. et al. (2012) Notch-RBP-J-Independent Marginal Zone B Cell Development in IgH Transgenic Mice with V(H) Derived from a Natural Polyreactive Antibody.
    PLoS One. 7: e38894.
  29. Matsuda T et al. (2015) The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.
    Immun Ageing. 12: 1.
  30. Funakoshi, S. et al. (2015) BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.
    PLoS One. 10 (1): e0117566.
  31. Xing Y et al. (2015) Positive Selection of Natural Poly-Reactive B Cells in the Periphery Occurs Independent of Heavy Chain Allelic Inclusion.
    PLoS One. 10 (5): e0125747.
  32. Carnrot, C. et al. (2011) Marginal zone B cells are naturally reactive to collagen type II and are involved in the initiation of the immune response in collagen-induced arthritis.
    Cell Mol Immunol. 8 (4): 296-304.
  33. Ding, Z. et al. (2016) IgE-mediated enhancement of CD4(+) T cell responses requires antigen presentation by CD8α(-) conventional dendritic cells.
    Sci Rep. 6: 28290.
  34. Bradford, B.M. et al. (2016) Prion pathogenesis is unaltered following down-regulation of SIGN-R1.
    Virology. 497: 337-345.
  35. Awasthi, A. et al. (2010) Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes.
    J Exp Med. 207: 1923-38.
  36. Flores, M. et al. (2015) FcγRIIB prevents inflammatory type I IFN production from plasmacytoid dendritic cells during a viral memory response.
    J Immunol. 194 (9): 4240-50.
  37. Oh, D.S. et al. (2017) Transient Depletion of CD169+ Cells Contributes to Impaired Early Protection and Effector CD8+ T Cell Recruitment against Mucosal Respiratory Syncytial Virus Infection.
    Front Immunol. 8: 819.
  38. Bogie, J.F. et al. (2018) CD169 is a marker for highly pathogenic phagocytes in multiple sclerosis.
    Mult Scler. 24 (3): 290-300.
  39. Tsai, C.Y. et al. (2018) Bystander inhibition of humoral immune responses by Epstein-Barr virus LMP1.
    Int Immunol. 30 (12): 579-90.
  40. Groh, J. et al. (2021) Immune modulation attenuates infantile neuronal ceroid lipofuscinosis in mice before and after disease onset
    Brain Communications. fcab047 [Epub ahead of print].
  41. Vanderkerken, M. et al. (2020) TAO-kinase 3 governs the terminal differentiation of NOTCH2-dependent splenic conventional dendritic cells.
    Proc Natl Acad Sci U S A. 117 (49): 31331-31342.
  42. Dekker, J.D. et al. (2019) Loss of the FOXP1 Transcription Factor Leads to Deregulation of B Lymphocyte Development and Function at Multiple Stages.
    Immunohorizons. 3 (10): 447-62.

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Synonyms
Sialoadhesin
UniProt
Q62230
Entrez Gene
Siglec1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005529 sugar binding
GO:0006897 endocytosis
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