CD169 antibody | MOMA-1

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Rat anti Mouse CD169:Alexa Fluor® 647

Rat anti Mouse CD169:FITC

Rat anti Mouse CD169

Rat anti Mouse CD169

Product Type
Monoclonal Antibody
Clone
MOMA-1
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA947A647 F 100 Tests/1ml
MCA947F F IF 0.1 mg
MCA947G C F IF 0.25 mg
MCA947GA C F IF 0.1 mg
MCA947 C F IF 2 ml
Rat anti Mouse CD169, clone MOMA-1 recognizes murine CD169, also known as sialoadhesin or Siglec-1. CD169 is a lectin-like receptor expressed by certain populations of macrophages including marginal zone metallophils of the spleen, subcapsular macrophages of lymph nodes and stromal macrophages in bone marrow (Morris et al. 1991).

CD169 is a ~185 kDa sialic acid binding receptor containing 17 immunoglobulin-like domains (Crocker et al. 1992). Expression of CD169 can be induced on macrophages in culture by a serum factor and further modulated by cytokine exposure (McWilliam et al. 1992).

Rat anti mouse CD169, clone MOMA-1 has been used for the in vivo depletion of specific macrophage populations (Kraal et al. 1988).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Tissue Culture Supernatant - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Stromal (reticular) elements from mouse lymph nodes.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from hyperimmunized mice were fused with cells from the murine SP2/0 myeloma.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q62230 Related reagents
Entrez Gene
Siglec1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005529 sugar binding
GO:0006897 endocytosis
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD169 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Immunofluorescence
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Lymphoid tissue

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 647 MCA1212A647 100 Tests/1ml F
Rat IgG2a Negative Control:FITC MCA1212F 100 Tests F
Rat IgG2a Negative Control MCA1212 1 ml E F
Rat IgG2a Negative Control MCA1212 1 ml E F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for CD169 antibody

  1. Kraal, G. and Janse, M. (1986) Marginal metallophilic cells of the mouse spleen identified by a monoclonal antibody.
    Immunology. 58: 665-9.
  2. Oetke, C. et al. (2006) The antigen recognized by MOMA-I is sialoadhesin.
    Immunol Lett. 106: 96-98.
  3. Tumanov, A.V. et al. (2010) Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs.
    Blood. 116 (18): 3456-64.
  4. Karlsson, M.C. et al. (2003) Macrophages control the retention and trafficking of B lymphocytes in the splenic marginal zone.
    J Exp Med. 198: 333-40.
  5. Kanayama, N. et al. (2005) Analysis of marginal zone B cell development in the mouse with limited B cell diversity: role of the antigen receptor signals in the recruitment of B cells to the marginal zone.
    J Immunol. 174 (3): 1438-45.
  6. Höpken, U.E. et al. (2004) Distinct and overlapping roles of CXCR5 and CCR7 in B-1 cell homing and early immunity against bacterial pathogens.
    J Leukoc Biol. 76 (3): 709-18.
  7. Ferguson, A.R. et al. (2004) Marginal zone B cells transport and deposit IgM-containing immune complexes onto follicular dendritic cells.
    Int Immunol. 16 (10): 1411-22.
  8. Girkontaite, I. et al. (2004) The sphingosine-1-phosphate (S1P) lysophospholipid receptor S1P3 regulates MAdCAM-1+ endothelial cells in splenic marginal sinus organization.
    J Exp Med. 200 (11): 1491-501.
  9. Acevedo-Suárez, C.A. et al. (2005) Uncoupling of anergy from developmental arrest in anti-insulin B cells supports the development of autoimmune diabetes.
    J Immunol. 174 (2): 827-33.
  10. Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
    J Immunol. 186: 3441-51.
  11. Bhattacharyya, S. et al. (2011) NFATc1 affects mouse splenic B cell function by controlling the calcineurin-NFAT signaling network.
    J Exp Med. 208 (4): 823-39.
  12. Jang, I.K. et al. (2011) Growth-factor receptor-bound protein-2 (Grb2) signaling in B cells controls lymphoid follicle organization and germinal center reaction.
    Proc Natl Acad Sci U S A. 108: 7926-31.
  13. Rehm, A. et al. (2011) Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid organs.
    Blood. 118 (4): 1020-33.
  14. Mattsson, J. et al. (2011) Complement activation and complement receptors on follicular dendritic cells are critical for the function of a targeted adjuvant.
    J Immunol. 187: 3641-52.
  15. Whipple, E.C. et al. (2004) Analyses of the in vivo trafficking of stoichiometric doses of an anti-complement receptor 1/2 monoclonal antibody infused intravenously in mice.
    J Immunol. 173 (4): 2297-306.
  16. Zhang, Z. et al. (2012) Notch-RBP-J-Independent Marginal Zone B Cell Development in IgH Transgenic Mice with V(H) Derived from a Natural Polyreactive Antibody.
    PLoS One. 7: e38894.
  17. Matsuda T et al. (2015) The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.
    Immun Ageing. 12: 1.
  18. Funakoshi, S. et al. (2015) BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.
    PLoS One. 10 (1): e0117566.
  19. Xing Y et al. (2015) Positive Selection of Natural Poly-Reactive B Cells in the Periphery Occurs Independent of Heavy Chain Allelic Inclusion.
    PLoS One. 10 (5): e0125747.
  20. Carnrot, C. et al. (2011) Marginal zone B cells are naturally reactive to collagen type II and are involved in the initiation of the immune response in collagen-induced arthritis.
    Cell Mol Immunol. 8 (4): 296-304.
  21. Ding, Z. et al. (2016) IgE-mediated enhancement of CD4(+) T cell responses requires antigen presentation by CD8α(-) conventional dendritic cells.
    Sci Rep. 6: 28290.
  22. Bradford, B.M. et al. (2016) Prion pathogenesis is unaltered following down-regulation of SIGN-R1.
    Virology. 497: 337-345.
  23. Martinez-Picado, J. et al. (2016) Identification of Siglec-1 null individuals infected with HIV-1.
    Nat Commun. 7: 12412.
  24. Flores, M. et al. (2015) FcγRIIB prevents inflammatory type I IFN production from plasmacytoid dendritic cells during a viral memory response.
    J Immunol. 194 (9): 4240-50.
  25. Oh, D.S. et al. (2017) Transient Depletion of CD169+ Cells Contributes to Impaired Early Protection and Effector CD8+ T Cell Recruitment against Mucosal Respiratory Syncytial Virus Infection.
    Front Immunol. 8: 819.
  26. Bogie, J.F. et al. (2018) CD169 is a marker for highly pathogenic phagocytes in multiple sclerosis.
    Mult Scler. 24 (3): 290-300.