CD169 antibody | MOMA-1



















Rat anti Mouse CD169:FITC
Rat anti Mouse CD169
Rat anti Mouse CD169
- Product Type
- Monoclonal Antibody
- Clone
- MOMA-1
- Isotype
- IgG2a
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA947F | C IF | 0.1 mg |
![]() |
|
MCA947GA | C IF | 0.1 mg |
![]() |
|
MCA947G | C IF | 0.25 mg |
![]() |
|
MCA947 | C IF | 2 ml |
![]() |
CD169 is a ~185 kDa sialic acid binding receptor containing 17 immunoglobulin-like domains (Crocker et al. 1992). Expression of CD169 can be induced on macrophages in culture by a serum factor and further modulated by cytokine exposure (McWilliam et al. 1992).
Rat anti mouse CD169, clone MOMA-1 has been used for the in vivo depletion of specific macrophage populations (Kraal et al. 1988).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Stromal (reticular) elements from mouse lymph nodes.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from hyperimmunized mice were fused with cells from the murine SP2/0 myeloma.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q62230
- Entrez Gene
- Siglec1
- GO Terms
- GO:0005886 plasma membrane
- GO:0005515 protein binding
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0005529 sugar binding
- GO:0006897 endocytosis
- Regulatory
- For research purposes only
Applications of CD169 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunofluorescence | |||
Immunohistology - Frozen | 1/10 |
- Histology Positive Control Tissue
- Lymphoid tissue
- Histology Positive Control Tissue
- Lymphoid tissue
Secondary Antibodies Available
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
![]() |
|
Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
![]() |
Application Based External Images
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Product Specific References
References for CD169 antibody
-
Kraal, G. and Janse, M. (1986) Marginal metallophilic cells of the mouse spleen identified by a monoclonal antibody.
Immunology. 58: 665-9. -
Oetke, C. et al. (2006) The antigen recognized by MOMA-I is sialoadhesin.
Immunol Lett. 106: 96-98. -
Tumanov, A.V. et al. (2010) Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs.
Blood. 116 (18): 3456-64. -
Karlsson, M.C. et al. (2003) Macrophages control the retention and trafficking of B lymphocytes in the splenic marginal zone.
J Exp Med. 198: 333-40. -
Kanayama, N. et al. (2005) Analysis of marginal zone B cell development in the mouse with limited B cell diversity: role of the antigen receptor signals in the recruitment of B cells to the marginal zone.
J Immunol. 174 (3): 1438-45. -
Höpken, U.E. et al. (2004) Distinct and overlapping roles of CXCR5 and CCR7 in B-1 cell homing and early immunity against bacterial pathogens.
J Leukoc Biol. 76 (3): 709-18. -
Ferguson, A.R. et al. (2004) Marginal zone B cells transport and deposit IgM-containing immune complexes onto follicular dendritic cells.
Int Immunol. 16 (10): 1411-22. -
Girkontaite, I. et al. (2004) The sphingosine-1-phosphate (S1P) lysophospholipid receptor S1P3 regulates MAdCAM-1+ endothelial cells in splenic marginal sinus organization.
J Exp Med. 200 (11): 1491-501. -
Acevedo-Suárez, C.A. et al. (2005) Uncoupling of anergy from developmental arrest in anti-insulin B cells supports the development of autoimmune diabetes.
J Immunol. 174 (2): 827-33. -
Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
J Immunol. 186: 3441-51. -
Bhattacharyya, S. et al. (2011) NFATc1 affects mouse splenic B cell function by controlling the calcineurin-NFAT signaling network.
J Exp Med. 208 (4): 823-39. -
Jang, I.K. et al. (2011) Growth-factor receptor-bound protein-2 (Grb2) signaling in B cells controls lymphoid follicle organization and germinal center reaction.
Proc Natl Acad Sci U S A. 108: 7926-31. -
Rehm, A. et al. (2011) Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid organs.
Blood. 118 (4): 1020-33. -
Mattsson, J. et al. (2011) Complement activation and complement receptors on follicular dendritic cells are critical for the function of a targeted adjuvant.
J Immunol. 187: 3641-52. -
Whipple, E.C. et al. (2004) Analyses of the in vivo trafficking of stoichiometric doses of an anti-complement receptor 1/2 monoclonal antibody infused intravenously in mice.
J Immunol. 173 (4): 2297-306. -
Zhang, Z. et al. (2012) Notch-RBP-J-Independent Marginal Zone B Cell Development in IgH Transgenic Mice with V(H) Derived from a Natural Polyreactive Antibody.
PLoS One. 7: e38894. -
Matsuda T et al. (2015) The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.
Immun Ageing. 12: 1. -
Funakoshi, S. et al. (2015) BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.
PLoS One. 10 (1): e0117566. -
Xing Y et al. (2015) Positive Selection of Natural Poly-Reactive B Cells in the Periphery Occurs Independent of Heavy Chain Allelic Inclusion.
PLoS One. 10 (5): e0125747. -
Carnrot, C. et al. (2011) Marginal zone B cells are naturally reactive to collagen type II and are involved in the initiation of the immune response in collagen-induced arthritis.
Cell Mol Immunol. 8 (4): 296-304. -
Ding, Z. et al. (2016) IgE-mediated enhancement of CD4(+) T cell responses requires antigen presentation by CD8α(-) conventional dendritic cells.
Sci Rep. 6: 28290. -
Bradford, B.M. et al. (2016) Prion pathogenesis is unaltered following down-regulation of SIGN-R1.
Virology. 497: 337-345. -
Awasthi, A. et al. (2010) Rap1b facilitates NK cell functions via IQGAP1-mediated signalosomes.
J Exp Med. 207: 1923-38. -
Flores, M. et al. (2015) FcγRIIB prevents inflammatory type I IFN production from plasmacytoid dendritic cells during a viral memory response.
J Immunol. 194 (9): 4240-50. -
Oh, D.S. et al. (2017) Transient Depletion of CD169+ Cells Contributes to Impaired Early Protection and Effector CD8+ T Cell Recruitment against Mucosal Respiratory Syncytial Virus Infection.
Front Immunol. 8: 819. -
Bogie, J.F. et al. (2018) CD169 is a marker for highly pathogenic phagocytes in multiple sclerosis.
Mult Scler. 24 (3): 290-300.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra