CD16-2 antibody | AT137

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Rat anti Mouse CD16-2:FITC

Rat anti Mouse CD16-2

Product Type
Monoclonal Antibody
Clone
AT137
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA5999F
Datasheet
F
SDS
0.1 mg loader

MCA5999
Datasheet
C F
SDS
0.25 mg loader

Rat anti mouse CD16-2 antibody, clone AT137, recognizes CD16-2, also known as FcRIV. CD16-2 is a low affinity activatory receptor that binds to IgG2a and IgG2b with intermediate affinity. FcRIV has no affinity for IgG1 or IgG3 and therefore has a unique subclass specificity when compared to other Fc receptors. Expression of CD16-2 is restricted to cells of the myeloid lineage.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Fusion Protein consisting of mouse CD16-2 and rat CD4
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of the NS-1 myeloma cell line

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q8R477
Regulatory
For research purposes only

Applications of CD16-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/10
Immunohistology - Frozen 1/100 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
Rat IgG2a Negative Control MCA1212 E F 1 ml loader

Product Specific References

References for CD16-2 antibody

  1. White, A.L. et al. (2011) Interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 monoclonal antibody.
    J Immunol. 187 (4): 1754-63.
  2. Lunnon, K. et al. (2011) Systemic inflammation modulates Fc receptor expression on microglia during chronic neurodegeneration.
    J Immunol. 186: 7215-24
  3. Tutt AL et al. (2015) Development and Characterization of Monoclonal Antibodies Specific for Mouse and Human Fcγ Receptors.
    J Immunol. 195 (11): 5503-16.

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