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CD134 antibody | OX-86

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Anti Mouse CD134 Antibody, clone OX-86 thumbnail image 1 Anti Mouse CD134 Antibody, clone OX-86 thumbnail image 2 Anti Mouse CD134 Antibody, clone OX-86 thumbnail image 3 Anti Mouse CD134 Antibody, clone OX-86 thumbnail image 4
Anti Mouse CD134 Antibody, clone OX-86 gallery image 1
Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse CD134 (MCA1420).
Anti Mouse CD134 Antibody, clone OX-86 gallery image 2
Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse CD134:Alexa Fluor® 647 (MCA1420A647).
Anti Mouse CD134 Antibody, clone OX-86 gallery image 3
Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse CD134:RPE (MCA1420PE).
Anti Mouse CD134 Antibody, clone OX-86 gallery image 4
Staining of stimulated rat spleen cells with Rat anti Mouse CD134:Azide Free (MCA1420XZ).

Rat anti Mouse CD134:FITC

Rat anti Mouse CD134

Rat anti Mouse CD134:RPE

Product Type
Monoclonal Antibody
Clone
OX-86
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1420F
Datasheet
F
SDS
0.1 mg loader

MCA1420FA
Datasheet
F
SDS
50 µg loader

MCA1420FB
Datasheet
F
SDS
0.5 mg loader

MCA1420
Datasheet
C F
SDS
0.25 mg loader

MCA1420GA
Datasheet
C F
SDS
0.1 mg loader

MCA1420PE
Datasheet
F
SDS
100 Tests loader

Rat anti Mouse CD134 antibody, clone OX-86 recognizes murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.

Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
CHO cells transfected with murine OX40.
Approx. Protein Concentrations
Pack Size: 0.1 mg, 50 µg
IgG concentration 0.1 mg/ml
Pack Size: 0.5 mg
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
Pack Size: 0.25 mg
IgG concentration 1.0 mg/ml
Pack Size: 0.1 mg
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized AO rats were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P47741
Entrez Gene
Tnfrsf4
GO Terms
GO:0016021 integral to membrane
GO:0005031 tumor necrosis factor receptor activity
GO:0006954 inflammatory response
GO:0006968 cellular defense response
GO:0009897 external side of plasma membrane
GO:0042981 regulation of apoptosis
GO:0030890 positive regulation of B cell proliferation
GO:0032582 negative regulation of gene-specific transcription
GO:0043433 negative regulation of transcription factor activity
GO:0045859 regulation of protein kinase activity
GO:0050710 negative regulation of cytokine secretion
GO:0051024 positive regulation of immunoglobulin secretion
Regulatory
For research purposes only

Applications of CD134 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

References for CD134 antibody

  1. Higgins, L.M. et al. (1999) Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein.
    J Immunol. 162 (1): 486-93.
  2. Lavelle, E.C. et al. (2003) Cholera toxin promotes the induction of regulatory T cells specific for bystander antigens by modulating dendritic cell activation.
    J Immunol. 171 (5): 2384-92.
  3. Lee, S.J. et al. (2004) 4-1BB and OX40 dual costimulation synergistically stimulate primary specific CD8 T cells for robust effector function.
    J Immunol. 173 (5): 3002-12.
  4. Maenz, M. et al. (2011) A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.
    Mol Vis. 17: 420-9.
  5. Zaini, J. et al. (2007) OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice.
    J Clin Invest. 117: 3330-8.
  6. Andarini, S. et al. (2004) Adenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.
    Cancer Res. 64: 3281-7.
  7. Malmström, V. et al. (2001) CD134L expression on dendritic cells in the mesenteric lymph nodes drives colitis in T cell-restored SCID mice.
    J Immunol. 166: 6972-81.
  8. McHugh, R.S. et al. (2002) CD4(+)CD25(+) immunoregulatory T cells: gene expression analysis reveals a functional role for the glucocorticoid-induced TNF receptor.
    Immunity. 16: 311-23.
  9. Thaunat, O. et al. (2010) Immune responses elicited in tertiary lymphoid tissues display distinctive features.
    PLoS One. 5: e11398.
  10. Wythe, S.E. et al. (2012) OX40 Ligand and Programmed Cell Death 1 Ligand 2 Expression on Inflammatory Dendritic Cells Regulates CD4 T Cell Cytokine Production in the Lung during Viral Disease
    J Immunol. 188: 1647-55.
  11. Reynolds, C. et al. (2014) Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.
    BMC Biol. 12: 32.

Further Reading

  1. Takeda, K. et al. (2000) CD27-mediated activation of murine NK cells.
    J Immunol. 164 (4): 1741-5.

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