CD134 antibody | OX-86
Rat anti Mouse CD134:FITC
Rat anti Mouse CD134
Rat anti Mouse CD134:RPE
- Product Type
- Monoclonal Antibody
- Clone
- OX-86
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA1420FA | F | 50 µg | ||||
| MCA1420F | F | 0.1 mg | ||||
| MCA1420FB | F | 0.5 mg | ||||
| MCA1420GA | C F | 0.1 mg | ||||
| MCA1420 | C F | 0.25 mg | ||||
| MCA1420PE | F | 100 Tests |
Rat anti Mouse CD134 antibody, clone OX-86 recognizes murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.
Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes.
Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- CHO cells transfected with murine OX40.
- Approx. Protein Concentrations
- Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- Pack Size: 0.1 mgIgG concentration 1 mg/mlPack Size: 0.25 mgIgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized AO rats were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Pack Size: 50 µg, 0.1 mgStore at +4oC for one month or at -20oC for longer.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of reconstitution.
More Information
- UniProt
- P47741 Related reagents
- Entrez Gene
- Tnfrsf4 Related reagents
- GO Terms
- GO:0016021 integral to membrane
- GO:0005031 tumor necrosis factor receptor activity
- GO:0006954 inflammatory response
- GO:0006968 cellular defense response
- GO:0009897 external side of plasma membrane
- GO:0042981 regulation of apoptosis
- GO:0030890 positive regulation of B cell proliferation
- GO:0032582 negative regulation of gene-specific transcription
- GO:0043433 negative regulation of transcription factor activity
- GO:0045859 regulation of protein kinase activity
- GO:0050710 negative regulation of cytokine secretion
- GO:0051024 positive regulation of immunoglobulin secretion
- Regulatory
- For research purposes only
Applications of CD134 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/10 | 1/50 | |
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | 1 ml | C E P WB | ||
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | 0.5 mg | C E IF P WB | ||
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | 0.1 mg | F IF WB | ||
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | 1 mg | F | ||
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | 1 mg | C E P RE | ||
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | 0.5 ml | F | ||
| Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) | STAR71D649GA | 0.1 mg | F IF | ||
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | 0.1 mg | F IF WB | ||
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | 0.5 mg | C E P | ||
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | 0.5 ml | F |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG1 Negative Control:FITC | MCA1211F | 100 Tests | F | ||
| Rat IgG1 Negative Control | MCA1211 | 1 ml | C E F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F |
Product Specific References
References for CD134 antibody
-
Higgins, L.M. et al. (1999) Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein.
J Immunol. 162 (1): 486-93. -
Lavelle, E.C. et al. (2003) Cholera toxin promotes the induction of regulatory T cells specific for bystander antigens by modulating dendritic cell activation.
J Immunol. 171 (5): 2384-92. -
Lee, S.J. et al. (2004) 4-1BB and OX40 dual costimulation synergistically stimulate primary specific CD8 T cells for robust effector function.
J Immunol. 173 (5): 3002-12. -
Maenz, M. et al. (2011) A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.
Mol Vis. 17: 420-9. -
Zaini, J. et al. (2007) OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice.
J Clin Invest. 117: 3330-8. -
Andarini, S. et al. (2004) Adenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.
Cancer Res. 64: 3281-7. -
Malmström, V. et al. (2001) CD134L expression on dendritic cells in the mesenteric lymph nodes drives colitis in T cell-restored SCID mice.
J Immunol. 166: 6972-81. -
McHugh, R.S. et al. (2002) CD4(+)CD25(+) immunoregulatory T cells: gene expression analysis reveals a functional role for the glucocorticoid-induced TNF receptor.
Immunity. 16: 311-23. -
Thaunat, O. et al. (2010) Immune responses elicited in tertiary lymphoid tissues display distinctive features.
PLoS One. 5: e11398. -
Wythe, S.E. et al. (2012) OX40 Ligand and Programmed Cell Death 1 Ligand 2 Expression on Inflammatory Dendritic Cells Regulates CD4 T Cell Cytokine Production in the Lung during Viral Disease
J Immunol. 188: 1647-55. -
Reynolds, C. et al. (2014) Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.
BMC Biol. 12: 32.
Further Reading
-
Takeda, K. et al. (2000) CD27-mediated activation of murine NK cells.
J Immunol. 164 (4): 1741-5.
