CD134 antibody | OX-86
Rat anti Mouse CD134
- Product Type
- Monoclonal Antibody
- Clone
- OX-86
- Isotype
- IgG1
- Specificity
- CD134
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD134 antibody, clone OX-86 recognizes murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- CHO cells transfected with murine OX40.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized AO rats were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/50 |
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD134 antibody
-
Higgins, L.M. et al. (1999) Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein.
J Immunol. 162 (1): 486-93. -
Malmström, V. et al. (2001) CD134L expression on dendritic cells in the mesenteric lymph nodes drives colitis in T cell-restored SCID mice.
J Immunol. 166: 6972-81. -
McHugh, R.S. et al. (2002) CD4(+)CD25(+) immunoregulatory T cells: gene expression analysis reveals a functional role for the glucocorticoid-induced TNF receptor.
Immunity. 16: 311-23. -
Lavelle, E.C. et al. (2003) Cholera toxin promotes the induction of regulatory T cells specific for bystander antigens by modulating dendritic cell activation.
J Immunol. 171 (5): 2384-92. -
Andarini, S. et al. (2004) Adenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.
Cancer Res. 64: 3281-7. -
Lee, S.J. et al. (2004) 4-1BB and OX40 dual costimulation synergistically stimulate primary specific CD8 T cells for robust effector function.
J Immunol. 173 (5): 3002-12. -
Zaini, J. et al. (2007) OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice.
J Clin Invest. 117: 3330-8. -
Thaunat, O. et al. (2010) Immune responses elicited in tertiary lymphoid tissues display distinctive features.
PLoS One. 5: e11398.
View The Latest Product References
-
Maenz, M. et al. (2011) A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.
Mol Vis. 17: 420-9. -
Wythe, S.E. et al. (2012) OX40 Ligand and Programmed Cell Death 1 Ligand 2 Expression on Inflammatory Dendritic Cells Regulates CD4 T Cell Cytokine Production in the Lung during Viral Disease
J Immunol. 188: 1647-55. -
Reynolds, C. et al. (2014) Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.
BMC Biol. 12: 32.
Further Reading
-
Takeda, K. et al. (2000) CD27-mediated activation of murine NK cells.
J Immunol. 164 (4): 1741-5.
- Synonyms
- OX40
- RRID
- AB_321575
- UniProt
- P47741
- Entrez Gene
- Tnfrsf4
- GO Terms
- GO:0016021 integral to membrane
- GO:0005031 tumor necrosis factor receptor activity
- GO:0006954 inflammatory response
- GO:0006968 cellular defense response
- GO:0009897 external side of plasma membrane
- GO:0042981 regulation of apoptosis
- GO:0030890 positive regulation of B cell proliferation
- GO:0032582 negative regulation of gene-specific transcription
- GO:0043433 negative regulation of transcription factor activity
- View More GO Terms
- GO:0045859 regulation of protein kinase activity
- GO:0050710 negative regulation of cytokine secretion
- GO:0051024 positive regulation of immunoglobulin secretion
MCA1420
147818
169203
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