CD11b antibody | M1/70.15
Rat anti MouseCD11b antibody, clone M1/70.15 (MCA74G) used to identify murine microglia in vitro by immunofluorescence.
Image caption:
Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.
From: Ferger AI, Campanelli L, Reimer V, Muth KN, Merdian I, Ludolph AC, Witting A.
Effects of mitochondrial dysfunction on the immunological properties of microglia.
J Neuroinflammation. 2010 Aug 11;7:45.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for the localization of microglia in murine brain tissue sections by immunofluorescence
Image caption:
HCT1026 inhibits MPTP-induced increased reactive Mac-1+ microglia in striatum and SNpc. Ageing (9-11 month-old) C57Bl/6 mice fed with a control (ct) or HCT1026 diets (30 mg kg-1) starting at -10 d, underwent an MPTP treatment, as described. At different time-intervals mice were anesthetized and rapidly perfused, the brains processed for immunohistochemistry. Coronal sections at the level of the striatum and SNpc were stained with Mac-1-Ab to localize microglial cells. A-B: Reactive (ameboid-like) microglial cells were counted at different time-intervals after saline and MPTP injection (n = 4/experimental group) in mice fed with a ct or HCT1026 diets, within both Str (A-G) and SNpc (H-N). Differences were analyzed by ANOVA followed by Newman-Keuls test, and considered significant when p <0.05. **p <0.05 vs saline; *° p <0.05 vs MPTP mice fed with ct diet. B-E: Representative confocal images of Mac-1 staining (green) in saline (B), 3 d after MPTP in mice fed with ct diet (C, 20× and D, 40×) or HCT1026 (F, 20× and G, 40×). Insets (100×) show microglia morphologic appearance. I-N: Representative confocal images of Mac-1 staining (red) in SNpc. Note the high density of reactive Mac-1+ cells with rounded cell bodies and short, thick processes 3 d after MPTP administration in mice fed with a ct diet (J-K), as compared to Mac-1 microglia of mice fed with HCT1026 (M-N), exhibiting a more elongated cell body and long ramified processes.
From: L'Episcopo F, Tirolo C, Caniglia S, Testa N, Serra PA, Impagnatiello F, Morale MC, Marchetti B.
Combining nitric oxide release with anti-inflammatory activity preserves nigrostriatal dopaminergic innervation and prevents motor impairment in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease.
J Neuroinflammation. 2010 Nov 23;7:83.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for the localization of microglia in murine brain tissue sections by immunofluorescence
Image caption:
HCT1026 inhibits MPTP-induced increased Mac-1, PHOX, iNOX and 3-Nitrotyrosine expression in SNpc. A-B: Western blotting of phagocyte oxidase PHOX (A) and Mac-1 (B) within the VM at different time-intervals after saline or MPTP injection in mice fed with the control or HCT1026 diet. The data from experimental bands were normalized to β-actin, before statistical analysis of variance. Values are expressed as % of saline-injected controls. Differences were analyzed by ANOVA followed by Newman-Keuls test, and considered significant when p <0.05. * p <0.05 compared to saline; ° p <0.05 vs MPTP fed with the control diet. C. Semi-quantitative RT-PCR for iNOS. The 250 ng of cDNA were used for PCR, by using Super Taq DNA polymerase with specific primer pairs for iNOS (500 bp) and Classic GADPH Standard (270 bp). Samples from PCR reactions were processed as described. Fluorescent bands of amplified gene products were analyzed, the values normalized against GADPH and ratios expressed as % of control, within each experimental group (C), see text for details. Differences were analyzed by ANOVA as above. ** p <0.05 vs saline; ° p <0.05 vs MPTP + control diet. D: Mean numbers of Mac1+iNOS+ cells within the SNpc in saline and MPTP mice fed with the control or HCT1026 diet. Cell counts obtained as described in Material section. E-F: Representative confocal images showing double staining with Mac-1 (green) and iNOS (red) in MPTP mice fed with the control (E) or HCT1026 (F) diets. G: Percent (%) of TH+ neurons colocalizing with 3-nitrotyrosine (3-NT), a peroxynitrite footprint. Dual stained TH+ 3-NT+ neurons were counted as described and values expressed as % of total TH+ neurons. H-I: Representative confocal images showing dual immunostaining with 3-NT (green) and TH (red) in MPTP mice fed with a ct diet (H) showing that a large proportion of DAergic neurons colocalize (orange-to-yellow) as opposed to TH neurons of mice fed with HCT1026 (E) where no colocalization was observed in the large part of SNpc neurons.
From: L'Episcopo F, Tirolo C, Caniglia S, Testa N, Serra PA, Impagnatiello F, Morale MC, Marchetti B.
Combining nitric oxide release with anti-inflammatory activity preserves nigrostriatal dopaminergic innervation and prevents motor impairment in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease.
J Neuroinflammation. 2010 Nov 23;7:83.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti mouse CD11b antibody, clone M1/70.15 (MCA74G) used for the assessment of CD11b expression by activated N9 microglial cells using immunofluorescence.
Image caption:
Localization of CD11b and p-STAT3 immunoreactivity in activated N9 cells. Experiments were performed as described above. Confocal immunofluorescence microscopy was performed on cultures that were immunoreacted with antibodies against phospho-STAT3 tyr705 (left, rabbit monoclonal, 1:100; FITC-conjugated sheep anti- rabbit secondary, 1:200) and CD11b (right, as Figure 1 shows) at 12 h after EMF exposure (A-L). (A-C) Untreated cultures were used as a control. (D-F) Cultures pretreated with P6 (10 μM). (G-I) EMF induced more phosphorylation of STAT3 (green) and expression of CD11b (red) in N9 cells. (J-L) P6 inhibits the phosphorylation of STAT3 and expression of CD11b at 12 h after EMF exposure. (M-O) P6 pretreatment significantly suppresses the phosphorylation of STAT3, but a slight and significant increase of CD11b is still apparent at 3 h after EMF exposure. Scale bar 10 μm. (P) Bar graphs show semi-quantification of fluorescence intensity for p-STAT3 and CD11b in N9 cells. Results are presented as mean ± S.D. of three independent experiments. Statistical comparisons to control are indicated by * p <0.05; ** p <0.01.
From: Yang X, He G, Hao Y, Chen C, Li M, Wang Y, Zhang G, Yu Z.
The role of the JAK2-STAT3 pathway in pro-inflammatory responses of EMF-stimulated N9 microglial cells.
J Neuroinflammation. 2010 Sep 9;7:54.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
AlexaFluor®647 conjugated Rat ati mouse CD11b antibody, clone M1/70.15 (MCA74A647) used for the evaluation of CD11b expression on immortalized murine microglia by flow cytometry.expression
Image caption:
IMG cells display similar morphology to primary microglia and express the microglia markers CD11b and F4/80. a Representative DIC images of IMG, BV-2, and primary adult microglial cells. Images are at ×40 magnification. b Flow cytometry of IMG cells. Representative zebra plot (left panel) of IMG showing a population of >95 % was gated for the analysis of IMG F4/80 (middle panel) and CD11b (right panel) expression (filled trace) by flow cytometry. Isotype controls (open trace) are also included in the graphs
From: McCarthy RC, Lu DY, Alkhateeb A, Gardeck AM, Lee CH, Wessling-Resnick M.
Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta.
J Neuroinflammation. 2016 Jan 27;13:21.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for identification of CD11b expressing cells in murine sciatic nerves by immunofluorescence on cryosections.
Image caption:
Overexpression of ERp57 in transgenic mice increases axonal regeneration after peripheral nerve injury.
(A) Non-Tg and Tg-ERp57 mice were damaged and sciatic nerves were extracted at 7, 11 and 14 days post-injury (dpi). Transversal semi-thin sections were obtained from the distal region and remyelinated axons (white arrows) and degenerated myelins (black arrows) were analyzed. (B) Quantification of remyelinated axons and (C) degenerated myelin density was measured at 7, 11 and 14 days post-injury. (D) Tg- ERp57 and Non-Tg sciatic nerves were processed for immunofluorescence in uninjured conditions and at 14 dpi. Sciatic nerves were analyzed for MBP (red), Cd11b (green) and nuclei were stained using DAPI (blue). (E) The staining density for Cd11b was quantified 14 days after injury (right panel). Data is presented as mean ± S.E.M. * p <0.05, ** p < 0.01, *** p <0.001. For histological analysis, statistical differences were obtained using a student's t-test (n = 3 animals per group). Scale bar: 20μm.
From: Castillo V, Oñate M, Woehlbier U, Rozas P, Andreu C, Medinas D, et al. (2015)
Functional Role of the Disulfide Isomerase ERp57 in Axonal Regeneration.
PLoS ONE 10(9): e0136620.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for identification of CD11b expressing cells in murine sciatic nerves by immunofluorescence on cryosections.
Image caption:
ERp57 overexpression reduces axonal degeneration.
(A) Electron microscopy of Non-Tg and Tg-ERp57 uninjured and 14 days-damaged nerves. In uninjured conditions white arrowheads indicate axoplasm of myelinated fibers, black arrowheads, compact myelin sheaths and asterisks, unmyelinated fibers. At 14 days post-injury black arrows indicated degenerated myelins and white arrows, remyelinated axons. Scale bar: 4μm. (B) hERp57 mRNA levels were determined in macrophages isolated from alveoli of Non-Tg and Tg-ERp57 mice after cell sorting of Cd11b-positive cells using real-time PCR (n = 2 per group). Cortex tissue from these animals was used as positive control. (C) Non-Tg and Tg-ERp57 mice were damaged and sciatic nerves were extracted at 14 days post-injury. Contralateral uninjured nerves were used as control. Transversal slides were processed for immunofluorescence for FLAG (red), MBP (green) and DAPI (blue) to identify Schwann cells. (D) Animals described in C were used for immunofluorescence to stain FLAG (red), Cd11b (green) and DAPI (blue) to analyse the infiltrating macrophage population. At the right panels, magnifications of indicated areas are shown. Scale bar: 20 mm. In B data are shown as mean.
From: Castillo V, Oñate M, Woehlbier U, Rozas P, Andreu C, Medinas D, et al. (2015)
Functional Role of the Disulfide Isomerase ERp57 in Axonal Regeneration.
PLoS ONE 10(9): e0136620.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74GA) used for evaluation of macrophage infiltrates in murine ear tissue sections by immunohistochemistry on formalin fixed, paraffin embedded samples.
Image caption:
Protective effect of bvPLA2 on macrophage infiltration in DFE/DNCB-induced mice via CD206. The ear tissue sections obtained from wild-type (WT) mice (A) or CD206-deficient mice (CD206−/− mice) (A) were stained with IHC (magnification ×200). Arrows indicate infiltrated macrophages. The macrophage cell number count graph on the IHC stained sections (B). CON: normal control, AD: DFE/DNCB treatment, and AD + bvPLA2: DFE/DNCB treatment + bvPLA2 treatment. (*** p <0.001 versus Control; NS, ## p <0.01 versus AD).
From: Shin D, Choi W, Bae H.
Bee Venom Phospholipase A2 Alleviate House Dust Mite-Induced Atopic Dermatitis-Like Skin Lesions by the CD206 Mannose Receptor.
Toxins (Basel). 2018 Apr 2;10(4). pii: E146.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b:Alexa Fluor® 488
Rat anti Mouse CD11b:Alexa Fluor® 647
Rat anti Mouse CD11b:Biotin
Rat anti Mouse CD11b:FITC
Rat anti Mouse CD11b:Low Endotoxin
Rat anti Mouse CD11b:Pacific Blue®
Rat anti Mouse CD11b
Rat anti Mouse CD11b:RPE
Rat anti Mouse CD11b:RPE-Alexa Fluor® 647
Rat anti Mouse CD11b:RPE-Alexa Fluor® 750
Rat anti Mouse CD11b:RPE-Cy5
Rat anti Mouse CD11b:Alexa Fluor® 700
- Product Type
- Monoclonal Antibody
- Clone
- M1/70.15
- Isotype
- IgG2b
Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.
The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor (Beller et al. 1982).
Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue (Whiteland et al. 1995).
This product is routinely tested in flow cytometry on mouse peritoneal macrophages.
The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor (Beller et al. 1982).
Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue (Whiteland et al. 1995).
This product is routinely tested in flow cytometry on mouse peritoneal macrophages.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Pacific Blue - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilisedPack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin -Cy5 (RPE- Cy5) - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Reconstitution
- Pack Size: 25 TestsReconstitute with 0.25 ml distilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared from ascites or tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- T cell enriched splenocytes from B10 mice.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 25 TestsPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted.
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
- Guarantee
- 12 months from date of reconstitution.
- Guarantee
- 12 months from date of reconstitution.
- Guarantee
- 6 months from date of despatch
- Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P05555 Related reagents
- Entrez Gene
- Itgam Related reagents
- GO Terms
- GO:0001846 opsonin binding
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0005634 nucleus
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0008201 heparin binding
- GO:0009897 external side of plasma membrane
- GO:0030593 neutrophil chemotaxis
- GO:0043395 heparan sulfate proteoglycan binding
- GO:0045123 cellular extravasation
- GO:0050798 activated T cell proliferation
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- Cy® and CyDye® are registered trademarks of GE Healthcare
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD11b antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Functional Assays | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | 1/200 Pack Size: 0.1 mg 1/50 Pack Size: 0.25 mg |
|
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat | 1/10 |
- 1PLP fixation is recommended for optimal results, see Whiteland et al. for details
- 1PLP fixation is recommended for optimal results, see Whiteland et al. for details
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent. - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically via the Fc region to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent. - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically via the Fc region to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent. - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically via the Fc region to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent. - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically via the Fc region to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent. - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically via the Fc region to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent. - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically via the Fc region to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent. - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically via the Fc region to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent. - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2b Negative Control:RPE-Alexa Fluor® 750 | MCA6006P750 | 100 Tests/1ml | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F |
Application Based External Images
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Product Specific References
Source Reference
-
Springer, T. et al. (1978) Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.
Eur J Immunol. 8 (8): 539-51.
Antibody Characterization Reference
-
Springer, T. et al. (1979) Mac-1: a macrophage differentiation antigen identified by monoclonal antibody.
Eur J Immunol. 9 (4): 301-6.
References for CD11b antibody
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Beller, D.I. et al. (1982) Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor.
J Exp Med. 156 (4): 1000-9. -
Fernández-Suárez,D. (2014) The monoacylglycerol lipase inhibitor JZL184 is neuroprotective and alters glial cell phenotype in the chronic MPTP mouse model
Neurobiol Aging. 35: 2603-16. -
Welt, F.G. et al. (2000) Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries.
Arterioscler Thromb Vasc Biol. 20 (12): 2553-8. -
Terrando, N. et al. (2010) The impact of IL-1 modulation on the development of lipopolysaccharide-induced cognitive dysfunction.
Crit Care. 14 (3): R88. -
Redensek, A. et al. (2011) Expression and detrimental role of hematopoietic prostaglandin D synthase in spinal cord contusion injury.
Glia. 59: 603-14. -
Brochard, V. et al (2009) Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease.
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Chinnery, H.R. et al. (2010) Novel characterization of monocyte-derived cell populations in the meninges and choroid plexus and their rates of replenishment in bone marrow chimeric mice.
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Ferger, A.I. et al (2010) Effects of mitochondrial dysfunction on the immunological properties of microglia.
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Amantea, D. et al. (2016) Neuroprotective Properties of a Macrolide Antibiotic in a Mouse Model of Middle Cerebral Artery Occlusion: Characterization of the Immunomodulatory Effects and Validation of the Efficacy of Intravenous Administration.
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Werneburg, S. et al. (2016) Polysialylation and lipopolysaccharide-induced shedding of E-selectin ligand-1 and neuropilin-2 by microglia and THP-1 macrophages.
Glia. 64 (8): 1314-30. -
Certo, M. et al. (2015) Activation of RXR/PPARγ underlies neuroprotection by bexarotene in ischemic stroke.
Pharmacol Res. 102: 298-307. -
Chen, Z.Z. et al. (2016) Memantine mediates neuroprotection via regulating neurovascular unit in a mouse model of focal cerebral ischemia.
Life Sci. 150: 8-14. -
Rich, M.C. et al. (2016) Site-targeted complement inhibition by a complement receptor 2-conjugated inhibitor (mTT30) ameliorates post-injury neuropathology in mouse brains.
Neurosci Lett. 617: 188-94. -
McCarthy, R.C. et al. (2016) Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta.
J Neuroinflammation. 13: 21. -
Jones, R.S. et al. (2015) Inhibition of JAK2 attenuates the increase in inflammatory markers in microglia from APP/PS1 mice.
Neurobiol Aging. 36 (10): 2716-24. -
Amantea, D. et al. (2016) Azithromycin protects mice against ischemic stroke injury by promoting macrophage transition towards M2 phenotype.
Exp Neurol. 275 Pt 1: 116-25. -
Bains, M. & Roberts, J.L. (2016) Estrogen protects against dopamine neuron toxicity in primary mesencephalic cultures through an indirect P13K/Akt mediated astrocyte pathway.
Neurosci Lett. 610: 79-85. -
Ji, J. et al. (2015) Iptakalim protects against ischemic injury by improving neurovascular unit function in the mouse brain.
Clin Exp Pharmacol Physiol. 42 (7): 766-71. -
Kim, B.W. et al. (2015) α-Asarone attenuates microglia-mediated neuroinflammation by inhibiting NF kappa B activation and mitigates MPTP-induced behavioral deficits in a mouse model of Parkinson's disease.
Neuropharmacology. 97: 46-57. -
Nishikawa, K. et al. (2015) Resveratrol increases CD68⁺ Kupffer cells colocalized with adipose differentiation-related protein and ameliorates high-fat-diet-induced fatty liver in mice.
Mol Nutr Food Res. 59 (6): 1155-70. -
Jiang, H. et al. (2017) Dense Intra-adipose Sympathetic Arborizations Are Essential for Cold-Induced Beiging of Mouse White Adipose Tissue.
Cell Metab. 26 (4): 686-692.e3. -
Zhang, J.C. et al. (2017) Prophylactic effects of sulforaphane on depression-like behavior and dendritic changes in mice after inflammation.
J Nutr Biochem. 39: 134-44. -
Petković, F. et al. (2017) Reduced cuprizone-induced cerebellar demyelination in mice with astrocyte-targeted production of IL-6 is associated with chronically activated, but less responsive microglia.
J Neuroimmunol. 310: 97-102. -
Olesen, M. N. et al. (2018) CD4 T cells react to local increase of α-synuclein in a pathology-associated variant-dependent manner and modify brain microglia in absence of brain pathology
Heliyon. 4 (1): e00513. -
Shin, D. et al. (2018) Bee Venom Phospholipase A2 Alleviate House Dust Mite-Induced Atopic Dermatitis-Like Skin Lesions by the CD206 Mannose Receptor.
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