CD11b antibody | M1/70.15
Rat anti MouseCD11b antibody, clone M1/70.15 (MCA74G) used to identify murine microglia in vitro by immunofluorescence.
Image caption:
Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.
From: Ferger AI, Campanelli L, Reimer V, Muth KN, Merdian I, Ludolph AC, Witting A.
Effects of mitochondrial dysfunction on the immunological properties of microglia.
J Neuroinflammation. 2010 Aug 11;7:45.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for the localization of microglia in murine brain tissue sections by immunofluorescence
Image caption:
HCT1026 inhibits MPTP-induced increased reactive Mac-1+ microglia in striatum and SNpc. Ageing (9-11 month-old) C57Bl/6 mice fed with a control (ct) or HCT1026 diets (30 mg kg-1) starting at -10 d, underwent an MPTP treatment, as described. At different time-intervals mice were anesthetized and rapidly perfused, the brains processed for immunohistochemistry. Coronal sections at the level of the striatum and SNpc were stained with Mac-1-Ab to localize microglial cells. A-B: Reactive (ameboid-like) microglial cells were counted at different time-intervals after saline and MPTP injection (n = 4/experimental group) in mice fed with a ct or HCT1026 diets, within both Str (A-G) and SNpc (H-N). Differences were analyzed by ANOVA followed by Newman-Keuls test, and considered significant when p <0.05. **p <0.05 vs saline; *° p <0.05 vs MPTP mice fed with ct diet. B-E: Representative confocal images of Mac-1 staining (green) in saline (B), 3 d after MPTP in mice fed with ct diet (C, 20× and D, 40×) or HCT1026 (F, 20× and G, 40×). Insets (100×) show microglia morphologic appearance. I-N: Representative confocal images of Mac-1 staining (red) in SNpc. Note the high density of reactive Mac-1+ cells with rounded cell bodies and short, thick processes 3 d after MPTP administration in mice fed with a ct diet (J-K), as compared to Mac-1 microglia of mice fed with HCT1026 (M-N), exhibiting a more elongated cell body and long ramified processes.
From: L'Episcopo F, Tirolo C, Caniglia S, Testa N, Serra PA, Impagnatiello F, Morale MC, Marchetti B.
Combining nitric oxide release with anti-inflammatory activity preserves nigrostriatal dopaminergic innervation and prevents motor impairment in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease.
J Neuroinflammation. 2010 Nov 23;7:83.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for the localization of microglia in murine brain tissue sections by immunofluorescence
Image caption:
HCT1026 inhibits MPTP-induced increased Mac-1, PHOX, iNOX and 3-Nitrotyrosine expression in SNpc. A-B: Western blotting of phagocyte oxidase PHOX (A) and Mac-1 (B) within the VM at different time-intervals after saline or MPTP injection in mice fed with the control or HCT1026 diet. The data from experimental bands were normalized to β-actin, before statistical analysis of variance. Values are expressed as % of saline-injected controls. Differences were analyzed by ANOVA followed by Newman-Keuls test, and considered significant when p <0.05. * p <0.05 compared to saline; ° p <0.05 vs MPTP fed with the control diet. C. Semi-quantitative RT-PCR for iNOS. The 250 ng of cDNA were used for PCR, by using Super Taq DNA polymerase with specific primer pairs for iNOS (500 bp) and Classic GADPH Standard (270 bp). Samples from PCR reactions were processed as described. Fluorescent bands of amplified gene products were analyzed, the values normalized against GADPH and ratios expressed as % of control, within each experimental group (C), see text for details. Differences were analyzed by ANOVA as above. ** p <0.05 vs saline; ° p <0.05 vs MPTP + control diet. D: Mean numbers of Mac1+iNOS+ cells within the SNpc in saline and MPTP mice fed with the control or HCT1026 diet. Cell counts obtained as described in Material section. E-F: Representative confocal images showing double staining with Mac-1 (green) and iNOS (red) in MPTP mice fed with the control (E) or HCT1026 (F) diets. G: Percent (%) of TH+ neurons colocalizing with 3-nitrotyrosine (3-NT), a peroxynitrite footprint. Dual stained TH+ 3-NT+ neurons were counted as described and values expressed as % of total TH+ neurons. H-I: Representative confocal images showing dual immunostaining with 3-NT (green) and TH (red) in MPTP mice fed with a ct diet (H) showing that a large proportion of DAergic neurons colocalize (orange-to-yellow) as opposed to TH neurons of mice fed with HCT1026 (E) where no colocalization was observed in the large part of SNpc neurons.
From: L'Episcopo F, Tirolo C, Caniglia S, Testa N, Serra PA, Impagnatiello F, Morale MC, Marchetti B.
Combining nitric oxide release with anti-inflammatory activity preserves nigrostriatal dopaminergic innervation and prevents motor impairment in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease.
J Neuroinflammation. 2010 Nov 23;7:83.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti mouse CD11b antibody, clone M1/70.15 (MCA74G) used for the assessment of CD11b expression by activated N9 microglial cells using immunofluorescence.
Image caption:
Localization of CD11b and p-STAT3 immunoreactivity in activated N9 cells. Experiments were performed as described above. Confocal immunofluorescence microscopy was performed on cultures that were immunoreacted with antibodies against phospho-STAT3 tyr705 (left, rabbit monoclonal, 1:100; FITC-conjugated sheep anti- rabbit secondary, 1:200) and CD11b (right, as Figure 1 shows) at 12 h after EMF exposure (A-L). (A-C) Untreated cultures were used as a control. (D-F) Cultures pretreated with P6 (10 μM). (G-I) EMF induced more phosphorylation of STAT3 (green) and expression of CD11b (red) in N9 cells. (J-L) P6 inhibits the phosphorylation of STAT3 and expression of CD11b at 12 h after EMF exposure. (M-O) P6 pretreatment significantly suppresses the phosphorylation of STAT3, but a slight and significant increase of CD11b is still apparent at 3 h after EMF exposure. Scale bar 10 μm. (P) Bar graphs show semi-quantification of fluorescence intensity for p-STAT3 and CD11b in N9 cells. Results are presented as mean ± S.D. of three independent experiments. Statistical comparisons to control are indicated by * p <0.05; ** p <0.01.
From: Yang X, He G, Hao Y, Chen C, Li M, Wang Y, Zhang G, Yu Z.
The role of the JAK2-STAT3 pathway in pro-inflammatory responses of EMF-stimulated N9 microglial cells.
J Neuroinflammation. 2010 Sep 9;7:54.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
AlexaFluor®647 conjugated Rat ati mouse CD11b antibody, clone M1/70.15 (MCA74A647) used for the evaluation of CD11b expression on immortalized murine microglia by flow cytometry.expression
Image caption:
IMG cells display similar morphology to primary microglia and express the microglia markers CD11b and F4/80. a Representative DIC images of IMG, BV-2, and primary adult microglial cells. Images are at ×40 magnification. b Flow cytometry of IMG cells. Representative zebra plot (left panel) of IMG showing a population of >95 % was gated for the analysis of IMG F4/80 (middle panel) and CD11b (right panel) expression (filled trace) by flow cytometry. Isotype controls (open trace) are also included in the graphs
From: McCarthy RC, Lu DY, Alkhateeb A, Gardeck AM, Lee CH, Wessling-Resnick M.
Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta.
J Neuroinflammation. 2016 Jan 27;13:21.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for identification of CD11b expressing cells in murine sciatic nerves by immunofluorescence on cryosections.
Image caption:
Overexpression of ERp57 in transgenic mice increases axonal regeneration after peripheral nerve injury.
(A) Non-Tg and Tg-ERp57 mice were damaged and sciatic nerves were extracted at 7, 11 and 14 days post-injury (dpi). Transversal semi-thin sections were obtained from the distal region and remyelinated axons (white arrows) and degenerated myelins (black arrows) were analyzed. (B) Quantification of remyelinated axons and (C) degenerated myelin density was measured at 7, 11 and 14 days post-injury. (D) Tg- ERp57 and Non-Tg sciatic nerves were processed for immunofluorescence in uninjured conditions and at 14 dpi. Sciatic nerves were analyzed for MBP (red), Cd11b (green) and nuclei were stained using DAPI (blue). (E) The staining density for Cd11b was quantified 14 days after injury (right panel). Data is presented as mean ± S.E.M. * p <0.05, ** p < 0.01, *** p <0.001. For histological analysis, statistical differences were obtained using a student's t-test (n = 3 animals per group). Scale bar: 20μm.
From: Castillo V, Oñate M, Woehlbier U, Rozas P, Andreu C, Medinas D, et al. (2015)
Functional Role of the Disulfide Isomerase ERp57 in Axonal Regeneration.
PLoS ONE 10(9): e0136620.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for identification of CD11b expressing cells in murine sciatic nerves by immunofluorescence on cryosections.
Image caption:
ERp57 overexpression reduces axonal degeneration.
(A) Electron microscopy of Non-Tg and Tg-ERp57 uninjured and 14 days-damaged nerves. In uninjured conditions white arrowheads indicate axoplasm of myelinated fibers, black arrowheads, compact myelin sheaths and asterisks, unmyelinated fibers. At 14 days post-injury black arrows indicated degenerated myelins and white arrows, remyelinated axons. Scale bar: 4μm. (B) hERp57 mRNA levels were determined in macrophages isolated from alveoli of Non-Tg and Tg-ERp57 mice after cell sorting of Cd11b-positive cells using real-time PCR (n = 2 per group). Cortex tissue from these animals was used as positive control. (C) Non-Tg and Tg-ERp57 mice were damaged and sciatic nerves were extracted at 14 days post-injury. Contralateral uninjured nerves were used as control. Transversal slides were processed for immunofluorescence for FLAG (red), MBP (green) and DAPI (blue) to identify Schwann cells. (D) Animals described in C were used for immunofluorescence to stain FLAG (red), Cd11b (green) and DAPI (blue) to analyse the infiltrating macrophage population. At the right panels, magnifications of indicated areas are shown. Scale bar: 20 mm. In B data are shown as mean.
From: Castillo V, Oñate M, Woehlbier U, Rozas P, Andreu C, Medinas D, et al. (2015)
Functional Role of the Disulfide Isomerase ERp57 in Axonal Regeneration.
PLoS ONE 10(9): e0136620.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74GA) used for evaluation of macrophage infiltrates in murine ear tissue sections by immunohistochemistry on formalin fixed, paraffin embedded samples.
Image caption:
Protective effect of bvPLA2 on macrophage infiltration in DFE/DNCB-induced mice via CD206. The ear tissue sections obtained from wild-type (WT) mice (A) or CD206-deficient mice (CD206−/− mice) (A) were stained with IHC (magnification ×200). Arrows indicate infiltrated macrophages. The macrophage cell number count graph on the IHC stained sections (B). CON: normal control, AD: DFE/DNCB treatment, and AD + bvPLA2: DFE/DNCB treatment + bvPLA2 treatment. (*** p <0.001 versus Control; NS, ## p <0.01 versus AD).
From: Shin D, Choi W, Bae H.
Bee Venom Phospholipase A2 Alleviate House Dust Mite-Induced Atopic Dermatitis-Like Skin Lesions by the CD206 Mannose Receptor.
Toxins (Basel). 2018 Apr 2;10(4). pii: E146.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used to evaluate CD11b expression in murine BV2 microglial cells by immunofluorescence.
Image caption:
Immunofluorescence staining of polySia co-localized with the Golgi marker giantin. Cell shape is highlighted by co-staining with the microglia/macrophage marker CD11b.
From: Thiesler H, Beimdiek J, Hildebrandt H. Polysialic acid and Siglec-E orchestrate negative feedback regulation of microglia activation.
Cell Mol Life Sci. 2020 Jul 28 [Epub ahead of print].
doi: 10.1007/s00018-020-03601-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used to identify CD11b expressing microglia in murine optic nerve tissue by immunofluorescence.
Image caption:
Therapeutic fingolimod and teriflunomide treatment attenuates neuroinflammation in CLN1 mice.
(C) Representative immune fluorescence microscopy and quantification of CD11b+ and (D) Sn+ microglia in longitudinal optic nerve sections (n = 5 mice per group, One-way ANOVA and Tukey’s post hoc test). Blue and yellow lines indicate mean values from previously published preventive (from 1 to 6 months of age) fingolimod and teriflunomide treatment approaches, respectively. Scale bars: 20µm. *P <0.05, **P <0.01, ***P <0.001.
From: Janos Groh, Kristina Berve, Rudolf Martini,
Immune modulation attenuates infantile neuronal ceroid lipofuscinosis in mice before and after disease onset,
Brain Communications, 2021;, fcab047,
doi: 10.1093/braincomms/fcab047
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used to identify macrophages in murine sciatic nerve tissue by immunofluorescence.
Image caption:
Characterization of Wallerian degeneration in sciatic nerve after L3 spinal nerve transection. Toluidine-stained semi-thin transverse sections (first row) and images immunostained against CD11b (red) and MBP (green) or NFM (red) and P75NTR (green). DAPI (blue) was used as a nuclear stain. Control corresponds to a sciatic nerve segment of sham-operated animals. Scale bars, 40 μm. The dotted line shows the frontier between intact and degenerated areas of the Scn.
From: Lemaitre D, Hurtado ML, De Gregorio C, Oñate M, Martínez G, Catenaccio A, Wishart TM, Court FA.
Collateral Sprouting of Peripheral Sensory Neurons Exhibits a Unique Transcriptomic Profile.
Mol Neurobiol. 2020 Oct;57(10):4232-49.
doi: 10.1007/s12035-020-01986-3.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used to identify microglia in vitro by immunofluorescence.
Image caption:
Characterization of the mouse primary cortical neuron and BV2 microglia co-cultures and effects of neuroinflammation.
a) Immunofluorescence staining showed healthy MAP2-positive cortical DIV7 neurons in BV2 microglia co-cultures. Upper MAP2-positive (green) neurons and GFAP-positive (red) astrocytes. Lower panel shows CD11b-positive (red) BV2 microglia cells (white arrow) in MAP2-positive cortical neuron (green) and BV2 cell co-culture. Nuclei are stained with DAPI. Magnification × 20, scale bar 50 μm.
From: Martiskainen H, Paldanius KMA, Natunen T, Takalo M, Marttinen M, Leskelä S, Huber N, Mäkinen P, Bertling E, Dhungana H, Huuskonen M, Honkakoski P, Hotulainen P, Rilla K, Koistinaho J, Soininen H, Malm T, Haapasalo A, Hiltunen M.
DHCR24 exerts neuroprotection upon inflammation-induced neuronal death.
J Neuroinflammation. 2017 Nov 7;14(1):215.
doi: 10.1186/s12974-017-0991-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used to label microglia in mouse brain by immunofluorescence on cryostat sections.
Image caption:
Representative confocal images of Ago2 (red) expression by astrocytes (GFAP+) and microglia (CD11b+) (green) obtained in the neocortex and hippocampus of mice injected with saline (CTR) or 2 mg/kg lipopolysaccharide (LPS) (scale bar 10μm). Inserts highlight staining (scale bar 20μm).
From: Machado-Pereira M, Saraiva C, Bernardino L, Cristóvão AC, Ferreira R.
Argonaute-2 protects the neurovascular unit from damage caused by systemic inflammation.
J Neuroinflammation. 2022 Jan 6;19(1):11.
doi: 10.1186/s12974-021-02324-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
IF F C P ResetRat anti Mouse CD11b
- Product Type
- Monoclonal Antibody
- Clone
- M1/70.15
- Isotype
- IgG2b
- Specificity
- CD11b
Quick Links:
Antibody Quality and 
Unrivaled 
| Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor (Beller et al. 1982). Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue (Whiteland et al. 1995). This product is routinely tested in flow cytometry on mouse peritoneal macrophages. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- T cell enriched splenocytes from B10 mice.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/50 Pack Size: 0.25 mg 1/200 Pack Size: 0.1 mg |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 1 | |
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| Immunoprecipitation | |
- 1PLP fixation is recommended for optimal results, see Whiteland et al. for details
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
Source Reference
-
Springer, T. et al. (1978) Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.
Eur J Immunol. 8 (8): 539-51.
Antibody Characterization Reference
-
Springer, T. et al. (1979) Mac-1: a macrophage differentiation antigen identified by monoclonal antibody.
Eur J Immunol. 9 (4): 301-6. Eur J Immunol. 9 (4): 301-6.
References for CD11b antibody
-
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice.
J Neurosci. 30: 9651-8. -
Lefevre, L. et al. (2010) PPARγ ligands switched high fat diet-induced macrophage M2b polarization toward M2a thereby improving intestinal Candida elimination.
PLoS One. 5: e12828. -
Patel, P.C. and Harrison, R.E. (2008) Membrane ruffles capture C3bi-opsonized particles in activated macrophages.
Mol Biol Cell. 19: 4628-39. -
Zhang, H. et al. (2010) A p38 mitogen-activated protein kinase-dependent mechanism of disinhibition in spinal synaptic transmission induced by tumor necrosis factor-alpha.
J Neurosci. 30: 12844-55. -
Cunningham, K. et al. (2011) Conditional deletion of Ccm2 causes hemorrhage in the adult brain: a mouse model of human cerebral cavernous malformations.
Hum Mol Genet. 20: 3198-206. -
Khan, A.A. et al. (2015) Therapeutic Immunization with a Mixture of Herpes Simplex Virus Type 1 Glycoprotein D Derived "Asymptomatic" Human CD8+ T-cell Epitopes Decreases Spontaneous Ocular Shedding in Latently Infected HLA Transgenic Rabbits: Association with Low Frequency of Local PD-1+TIM-3+CD8+ Exhausted T Cells.
J Virol. pii: JVI.00788-15. -
Beller, D.I. et al. (1982) Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor.
J Exp Med. 156 (4): 1000-9.
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Shin, D. et al. (2018) Bee Venom Phospholipase A2 Alleviate House Dust Mite-Induced Atopic Dermatitis-Like Skin Lesions by the CD206 Mannose Receptor.
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J Neuroinflammation. 17 (1): 238. -
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Groh, J. et al. (2021) Immune modulation attenuates infantile neuronal ceroid lipofuscinosis in mice before and after disease onset
Brain Communications. fcab047 [Epub ahead of print]. -
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Paasila, P.J. et al. (2021) Ground state depletion microscopy as a tool for studying microglia-synapse interactions.
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- Synonyms
- Integrin Alpha M Chain
- MAC-1
- RRID
- AB_321293
- UniProt
- P05555
- Entrez Gene
- Itgam
- GO Terms
- GO:0001846 opsonin binding
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0005634 nucleus
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0008201 heparin binding
- GO:0009897 external side of plasma membrane
- GO:0030593 neutrophil chemotaxis
- View More GO Terms
- GO:0043395 heparan sulfate proteoglycan binding
- GO:0045123 cellular extravasation
- GO:0050798 activated T cell proliferation
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