Rat anti Mouse CD11a antibody, clone I21/7
recognizes murine CD11a, a ~170-180 kDa type I membrane glycoprotein, also known as the integrin alpha L subunit. CD11a is a member of the integrin family of adhesion molecules. CD11a is expressed on all leucocytes and associates with CD18 to form a heterodimer at the cell surface. The CD11a/CD18 heterodimer binds to intercellular adhesion molecules (ICAM); ICAM1, ICAM-2 and ICAM-3 and is involved in cell-cell adhesion processes.
Rat anti Mouse CD11a antibody, clone I21/7 is reported to block the cytolytic activity of cytotoxic T cell lines (Trowbridge and Omary 1981
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Whole BW5147 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
leukocyte cell-cell adhesion
integrin-mediated signaling pathway
external side of plasma membrane
positive regulation of cell-cell adhesion
positive regulation of T cell proliferation
activated T cell proliferation
positive regulation of calcium-mediated signaling
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD11a antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
Trowbridge, I.S. & Omary, M.B. (1981) Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1.
J Exp Med. 154 (5): 1517-24.
References for CD11a antibody
Champe, M. et al. (1995) Monoclonal antibodies that block the activity of leukocyte function-associated antigen 1 recognize three discrete epitopes in the inserted domain of CD11a.
J Biol Chem. 270 (3): 1388-94.
Barrett, T.B. et al. (1991) CD40 signaling activates CD11a/CD18 (LFA-1)-mediated adhesion in B cells.
J Immunol. 146 (6): 1722-9.
Talento, A. et al. (1993) A single administration of LFA-1 antibody confers prolonged allograft survival.
Transplantation. 55 (2): 418-22.
Campbell, S.B. et al. (2001) CD4 ligation promotes the IL-4-independent development of IL-4-producing clones from naive CD4(+) T cells.
J Immunol. 167 (10): 5610-9.
Kleinschnitz, C. et al. (2013) Regulatory T cells are strong promoters of acute ischemic stroke in mice by inducing dysfunction of the cerebral microvasculature.
Blood. 121: 679-691
Falanga, P.B. & Butcher, E.C. (1991) Late treatment with anti-LFA-1 (CD11a) antibody prevents cerebral malaria in a mouse model.
Eur J Immunol. 21 (9): 2259-63.
Chiu, W.C. et al. (2007) Effect of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in diabetic mice with sepsis.
Br J Nutr. 97 (4): 685-91.
Xumei, H. et al. (2020) Mesenchymal stem cell–derived exosomal microRNA-34c-5p ameliorates renal pericyte activation by inhibiting core fucosylation [preprint].
Nov 13. [Epub ahead of print].
Springer, T.A. et al. (1982) LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function.
Immunol Rev. 68: 171-95.
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