CD11a antibody | I21/7

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Rat anti Mouse CD11a

Product Type
Monoclonal Antibody
Clone
I21/7
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1368GT F IP 25 µg
MCA1368G F IP 0.25 mg
Rat anti Mouse CD11a antibody, clone I21/7 recognizes murine CD11a, a ~170-180 kDa type I membrane glycoprotein, also known as the integrin alpha L subunit. CD11a is a member of the integrin family of adhesion molecules. CD11a is expressed on all leucocytes and associates with CD18 to form a heterodimer at the cell surface. The CD11a/CD18 heterodimer binds to intercellular adhesion molecules (ICAM); ICAM1, ICAM-2 and ICAM-3 and is involved in cell-cell adhesion processes.

Rat anti Mouse CD11a antibody, clone I21/7 is reported to block the cytolytic activity of cytotoxic T cell lines (Trowbridge and Omary 1981).

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Whole BW5147 cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P24063 Related reagents
Entrez Gene
Itgal Related reagents
GO Terms
GO:0001772 immunological synapse
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0009897 external side of plasma membrane
GO:0022409 positive regulation of cell-cell adhesion
GO:0042102 positive regulation of T cell proliferation
GO:0050798 activated T cell proliferation
GO:0050850 positive regulation of calcium-mediated signaling
Regulatory
For research purposes only

Applications of CD11a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control MCA1212 1 ml E F

Product Specific References

Source Reference

  1. Trowbridge, I.S. & Omary, M.B. (1981) Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1.
    J Exp Med. 154 (5): 1517-24.

References for CD11a antibody

  1. Talento, A. et al. (1993) A single administration of LFA-1 antibody confers prolonged allograft survival.
    Transplantation. 55 (2): 418-22.
  2. Campbell, S.B. et al. (2001) CD4 ligation promotes the IL-4-independent development of IL-4-producing clones from naive CD4(+) T cells.
    J Immunol. 167 (10): 5610-9.
  3. Kleinschnitz, C. et al. (2013) Regulatory T cells are strong promoters of acute ischemic stroke in mice by inducing dysfunction of the cerebral microvasculature.
    Blood. 121: 679-691
  4. Falanga, P.B. & Butcher, E.C. (1991) Late treatment with anti-LFA-1 (CD11a) antibody prevents cerebral malaria in a mouse model.
    Eur J Immunol. 21 (9): 2259-63.
  5. Chiu, W.C. et al. (2007) Effect of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in diabetic mice with sepsis.
    Br J Nutr. 97 (4): 685-91.

Further Reading

  1. Springer, T.A. et al. (1982) LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function.
    Immunol Rev. 68: 171-95.