CD11a antibody | I21/7
Rat anti Mouse CD11a antibody, clone I21/7 recognizes murine CD11a, a ~170-180 kDa type I membrane glycoprotein, also known as the integrin alpha L subunit. CD11a is a member of the integrin family of adhesion molecules. CD11a is expressed on all leucocytes and associates with CD18 to form a heterodimer at the cell surface. The CD11a/CD18 heterodimer binds to intercellular adhesion molecules (ICAM); ICAM1, ICAM-2 and ICAM-3 and is involved in cell-cell adhesion processes.
Rat anti Mouse CD11a antibody, clone I21/7 is reported to block the cytolytic activity of cytotoxic T cell lines (Trowbridge and Omary 1981).
Rat anti Mouse CD11a antibody, clone I21/7 is reported to block the cytolytic activity of cytotoxic T cell lines (Trowbridge and Omary 1981).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Whole BW5147 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P24063
- Entrez Gene
- Itgal
- GO Terms
- GO:0001772 immunological synapse
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0022409 positive regulation of cell-cell adhesion
- GO:0042102 positive regulation of T cell proliferation
- GO:0050798 activated T cell proliferation
- GO:0050850 positive regulation of calcium-mediated signaling
- Regulatory
- For research purposes only
Applications of CD11a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Product Specific References
Source Reference
-
Trowbridge, I.S. & Omary, M.B. (1981) Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1.
J Exp Med. 154 (5): 1517-24.
References for CD11a antibody
-
Champe, M. et al. (1995) Monoclonal antibodies that block the activity of leukocyte function-associated antigen 1 recognize three discrete epitopes in the inserted domain of CD11a.
J Biol Chem. 270 (3): 1388-94. -
Barrett, T.B. et al. (1991) CD40 signaling activates CD11a/CD18 (LFA-1)-mediated adhesion in B cells.
J Immunol. 146 (6): 1722-9. -
Talento, A. et al. (1993) A single administration of LFA-1 antibody confers prolonged allograft survival.
Transplantation. 55 (2): 418-22. -
Campbell, S.B. et al. (2001) CD4 ligation promotes the IL-4-independent development of IL-4-producing clones from naive CD4(+) T cells.
J Immunol. 167 (10): 5610-9. -
Kleinschnitz, C. et al. (2013) Regulatory T cells are strong promoters of acute ischemic stroke in mice by inducing dysfunction of the cerebral microvasculature.
Blood. 121: 679-691 -
Falanga, P.B. & Butcher, E.C. (1991) Late treatment with anti-LFA-1 (CD11a) antibody prevents cerebral malaria in a mouse model.
Eur J Immunol. 21 (9): 2259-63. -
Chiu, W.C. et al. (2007) Effect of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in diabetic mice with sepsis.
Br J Nutr. 97 (4): 685-91. -
Xumei, H. et al. (2020) Mesenchymal stem cell–derived exosomal microRNA-34c-5p ameliorates renal pericyte activation by inhibiting core fucosylation [preprint].
Nov 13. [Epub ahead of print].
Further Reading
-
Springer, T.A. et al. (1982) LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function.
Immunol Rev. 68: 171-95.
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