CD11a antibody | I21/7

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Rat anti Mouse CD11a

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.25 mg loader

25 µg loader

Rat anti Mouse CD11a antibody, clone I21/7 recognizes murine CD11a, a ~170-180 kDa type I membrane glycoprotein, also known as the integrin alpha L subunit. CD11a is a member of the integrin family of adhesion molecules. CD11a is expressed on all leucocytes and associates with CD18 to form a heterodimer at the cell surface. The CD11a/CD18 heterodimer binds to intercellular adhesion molecules (ICAM); ICAM1, ICAM-2 and ICAM-3 and is involved in cell-cell adhesion processes.

Rat anti Mouse CD11a antibody, clone I21/7 is reported to block the cytolytic activity of cytotoxic T cell lines (Trowbridge and Omary 1981).

Product Details

Target Species
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Whole BW5147 cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0001772 immunological synapse
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0009897 external side of plasma membrane
GO:0022409 positive regulation of cell-cell adhesion
GO:0042102 positive regulation of T cell proliferation
GO:0050798 activated T cell proliferation
GO:0050850 positive regulation of calcium-mediated signaling
For research purposes only

Applications of CD11a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader

Product Specific References

Source Reference

  1. Trowbridge, I.S. & Omary, M.B. (1981) Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1.
    J Exp Med. 154 (5): 1517-24.

References for CD11a antibody

  1. Champe, M. et al. (1995) Monoclonal antibodies that block the activity of leukocyte function-associated antigen 1 recognize three discrete epitopes in the inserted domain of CD11a.
    J Biol Chem. 270 (3): 1388-94.
  2. Barrett, T.B. et al. (1991) CD40 signaling activates CD11a/CD18 (LFA-1)-mediated adhesion in B cells.
    J Immunol. 146 (6): 1722-9.
  3. Talento, A. et al. (1993) A single administration of LFA-1 antibody confers prolonged allograft survival.
    Transplantation. 55 (2): 418-22.
  4. Campbell, S.B. et al. (2001) CD4 ligation promotes the IL-4-independent development of IL-4-producing clones from naive CD4(+) T cells.
    J Immunol. 167 (10): 5610-9.
  5. Kleinschnitz, C. et al. (2013) Regulatory T cells are strong promoters of acute ischemic stroke in mice by inducing dysfunction of the cerebral microvasculature.
    Blood. 121: 679-691
  6. Falanga, P.B. & Butcher, E.C. (1991) Late treatment with anti-LFA-1 (CD11a) antibody prevents cerebral malaria in a mouse model.
    Eur J Immunol. 21 (9): 2259-63.
  7. Chiu, W.C. et al. (2007) Effect of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in diabetic mice with sepsis.
    Br J Nutr. 97 (4): 685-91.
  8. Xuemei, H. et al. (2020) Mesenchymal stem cell–derived exosomal microRNA-34c-5p ameliorates renal pericyte activation by inhibiting core fucosylation
    Res Sq 13 Oct [Epub ahead of print].
  9. Hu, X. et al. (2021) Bone marrow mesenchymal stem cell-derivedexosomal miR-34c-5p ameliorates RIF by inhibitingthe core fucosylation of multiple proteins.
    Mol Ther. S1525-0016(21)00519-0.

Further Reading

  1. Springer, T.A. et al. (1982) LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function.
    Immunol Rev. 68: 171-95.

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