CD117 antibody | 2B8

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Rat anti Mouse CD117:Alexa Fluor® 488

Rat anti Mouse CD117:Alexa Fluor® 647

Rat anti Mouse CD117

Rat anti Mouse CD117:RPE

Rat anti Mouse CD117:Starbright Violet 515

Product Type
Monoclonal Antibody
Clone
2B8
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA1365A488T
Datasheet
F
MSDS
25 Tests/0.25ml loader

MCA1365A488
Datasheet
F
MSDS
100 Tests/1ml loader

MCA1365A647T
Datasheet
F
MSDS
25 Tests/0.25ml loader

MCA1365A647
Datasheet
F
MSDS
100 Tests/1ml loader

MCA1365G
Datasheet
F
MSDS
0.25 mg loader

MCA1365PE
Datasheet
F
MSDS
100 Tests loader

MCA1365SBV515
Datasheet
F
MSDS
100 Tests/0.5ml loader

Rat anti Mouse CD117 antibody, clone 2B8 recognizes mouse CD117, a ~150 kDa cell surface molecule otherwise known as the Kit, or c-kit, receptor.

Rat anti Mouse CD117 antibody, clone 2B8 recognizes an epitope within the extracellular region of CD117. In mice, CD117 is predominantly expressed on multipotent haematopoietic stem cells from the adult bone marrow and foetal liver.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647- liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to StarBright Violet 515 - liquid
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Preparation
Purified IgG prepared by affinity chromatography on Protein G.
Preparation
Purified IgG prepared by affinity chromatography on Protein A.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
Carrier Free
Yes
Immunogen
IL-3 dependent mast cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Wistar rats were fused with cells of the Fox-NY mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P05532
Entrez Gene
Kit
GO Terms
GO:0005515 protein binding
GO:0002318 myeloid progenitor cell differentiation
GO:0002320 lymphoid progenitor cell differentiation
GO:0002573 myeloid leukocyte differentiation
GO:0016021 integral to membrane
GO:0005020 stem cell factor receptor activity
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005524 ATP binding
GO:0006687 glycosphingolipid metabolic process
GO:0006935 chemotaxis
GO:0007243 intracellular protein kinase cascade
GO:0007286 spermatid development
GO:0008284 positive regulation of cell proliferation
GO:0009314 response to radiation
GO:0009897 external side of plasma membrane
GO:0010628 positive regulation of gene expression
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0019221 cytokine-mediated signaling pathway
GO:0035234 germ cell programmed cell death
GO:0043069 negative regulation of programmed cell death
GO:0043406 positive regulation of MAP kinase activity
GO:0046777 protein autophosphorylation
GO:0048066 developmental pigmentation
GO:0048070 regulation of developmental pigmentation
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
Regulatory
For research purposes only

Applications of CD117 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat 1/10
Flow Cytometry 1/50 1/200
Flow Cytometry Neat
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Product Specific References

Source Reference

  1. Ikuta, K. & Weissman, I.L. (1992) Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation.
    Proc Natl Acad Sci U S A. 89 (4): 1502-6.

Further Reading

  1. Fleming WH et al. (1993) Steel factor influences the distribution and activity of murine hematopoietic stem cells in vivo.
    Proc Natl Acad Sci U S A. 90 (8): 3760-4.
  2. Rico-Vargas, S.A. et al. (1994) c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody.
    J Immunol. 152 (6): 2845-52.
  3. Manova, K. et al. (1992) c-kit receptor and ligand expression in postnatal development of the mouse cerebellum suggests a function for c-kit in inhibitory interneurons.
    J Neurosci. 12 (12): 4663-76.
  4. Maeda, H. et al. (1992) Requirement of c-kit for development of intestinal pacemaker system.
    Development. 116 (2): 369-75.
  5. Takagi, M. et al. (1992) Stimulation of mouse connective tissue-type mast cells by hemopoietic stem cell factor, a ligand for the c-kit receptor.
    J Immunol. 148 (11): 3446-53.
  6. de Vries, P. et al. (1992) Thymus reconstitution by c-kit-expressing hematopoietic stem cells purified from adult mouse bone marrow.
    J Exp Med. 176 (6): 1503-9.