Luciferase antibody | LUC-Y

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Mouse anti Luciferase

Product Type
Monoclonal Antibody
Clone
LUC-Y
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2076
Datasheet
C E F* IF WB
MSDS
0.1 mg loader

Mouse anti Luciferase antibody, clone LUC-Y detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.

Product Details

Target Species
Insect
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant luciferase expressed in E. coli.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c were fused with cells of the SP20 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P08659 Related reagents
GO Terms
GO:0005777 peroxisome
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0055114 oxidation-reduction process
GO:0008218 bioluminescence
GO:0047077 Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity
Regulatory
For research purposes only

Applications of Luciferase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/5000
Flow Cytometry 1 10ug/ml
Immunofluorescence
Immunohistology - Frozen
Western Blotting 1/100
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Western Blotting
MCA2076 detects a band of approximately 55 - 60 kDa with a sample of 1ug of purified luciferase from Photinus pyralis on a Western blot.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Immunofluorescence

Product Specific References

References for Luciferase antibody

  1. Laakkonen, J.P. et al. (2009) Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.
    PLoS One. 4:e5093.
  2. van Beusechem, V.W. et al. (2002) Efficient and selective gene transfer into primary human brain tumors by using single-chain antibody-targeted adenoviral vectors with native tropism abolished.
    J Virol. 76: 2753-62.
  3. Zhao, D. et al. (2008) Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI.
    FASEB J. 22: 2445-51.
  4. Spiotto, M.T. et al. (2010) Imaging the unfolded protein response in primary tumors reveals microenvironments with metabolic variations that predict tumor growth.
    Cancer Res. 70: 78-88.
  5. Howarth, J.L. et al. (2007) Hsp40 molecules that target to the ubiquitin-proteasome system decrease inclusion formation in models of polyglutamine disease.
    Mol Ther. 15: 1100-5.
  6. Keravala, A. et al. (2008) Long-term transgene expression in mouse neural progenitor cells modified with phiC31 integrase.
    J Neurosci Methods. 173: 299-305.
  7. Zhou, H. et al. (2009) Dynamic near-infrared optical imaging of 2-deoxyglucose uptake by intracranial glioma of athymic mice.
    PLoS One. 4: e8051.