Luciferase antibody | LUC-Y

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IF ResetMouse anti Luciferase
- Product Type
- Monoclonal Antibody
- Clone
- LUC-Y
- Isotype
- IgG1
- Specificity
- Luciferase
Mouse anti Luciferase antibody, clone LUC-Y detects luciferase of the North American firefly Photinus pyralis in cells and tissues. |
- Target Species
- Insect
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant luciferase expressed in E. coli.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c were fused with cells of the SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
1/500 | 1/5000 |
Flow Cytometry 1 | ![]() |
10μg/ml | |
Immunofluorescence | ![]() |
||
Immunohistology - Frozen | ![]() |
||
Western Blotting | ![]() |
1/100 |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
- Western Blotting
- Mouse anti luciferase antibody, clone LUC-Y detects a band of approximately 55 - 60 kDa with a sample of 1ug of purified luciferase from Photinus pyralis on a western blot.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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Description | Rabbit F(ab')2 anti Mouse IgG:RPE | ||||||
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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Description | Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | ||||||
Goat anti Mouse IgG:FITC (Rat Adsorbed) | STAR70 | F | 0.5 mg |
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Description | Goat anti Mouse IgG:FITC (Rat Adsorbed) | ||||||
Goat anti Mouse IgG:RPE (Rat Adsorbed) | STAR76 | F | 1 ml |
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Description | Goat anti Mouse IgG:RPE (Rat Adsorbed) | ||||||
Goat anti Mouse IgG:HRP (Rat Adsorbed) | STAR77 | C E P | 0.5 mg |
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Description | Goat anti Mouse IgG:HRP (Rat Adsorbed) | ||||||
Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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Description | Rabbit F(ab')2 anti Mouse IgG:FITC |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Description | Mouse IgG1 Negative Control |
References for Luciferase antibody
-
van Beusechem, V.W. et al. (2002) Efficient and selective gene transfer into primary human brain tumors by using single-chain antibody-targeted adenoviral vectors with native tropism abolished.
J Virol. 76: 2753-62. -
Howarth, J.L. et al. (2007) Hsp40 molecules that target to the ubiquitin-proteasome system decrease inclusion formation in models of polyglutamine disease.
Mol Ther. 15: 1100-5. -
Keravala, A. et al. (2008) Long-term transgene expression in mouse neural progenitor cells modified with phiC31 integrase.
J Neurosci Methods. 173: 299-305. -
Zhao, D. et al. (2008) Antivascular effects of combretastatin A4 phosphate in breast cancer xenograft assessed using dynamic bioluminescence imaging and confirmed by MRI.
FASEB J. 22: 2445-51. -
Zhou, H. et al. (2009) Dynamic near-infrared optical imaging of 2-deoxyglucose uptake by intracranial glioma of athymic mice.
PLoS One. 4: e8051. -
Laakkonen, J.P. et al. (2009) Clathrin-independent entry of baculovirus triggers uptake of E. coli in non-phagocytic human cells.
PLoS One. 4:e5093. -
Spiotto, M.T. et al. (2010) Imaging the unfolded protein response in primary tumors reveals microenvironments with metabolic variations that predict tumor growth.
Cancer Res. 70: 78-88.
- RRID
- AB_323475
- UniProt
- P08659
- GO Terms
- GO:0005777 peroxisome
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0055114 oxidation-reduction process
- GO:0008218 bioluminescence
- GO:0047077 Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity
MCA2076




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