Infliximab antibody | AbD19376_hIgG1

Human anti Infliximab:HRP

Product Type
Monoclonal Antibody
Clone
AbD19376_hIgG1
Isotype
IgG1
Specificity
Infliximab

Product Code Applications Pack Size List Price Your Price Qty
HCA216P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Human anti Infliximab, clone AbD19376_hIgG1, is an anti-idiotypic antibody that binds to both free infliximab and to infliximab bound to its target, TNFα. Clone AbD19376_hIgG1 does not inhibit the binding of infliximab to TNFα. It can be used in bioanalytical assays to measure the levels of infliximab and biosimilar products, such as Inflectra and Renflexis, in patient samples.

Clone AbD19376_hIgG1 binds to an epitope outside the infliximab binding site. This epitope is shared with other chimeric antibodies that are highly homologous to infliximab including the chimeric antibody cetuximab but not rituximab.

Human anti Infliximab, clone AbD19376_hIgG1 is a fully human antibody and may be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient sera. In the HRP-conjugated format, clone AbD19376_IgG1 is suitable as a detection antibody in pharmacokinetic (PK) assays to measure total infliximab levels (free drug, partially bound and fully bound).

Infliximab (branded as Remicade®) is a chimeric monoclonal antibody drug (IgG1/kappa) that has been approved for treatment of psoriasis, Crohn's disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis, and ulcerative colitis.

Infliximab is directed against Tumor Necrosis Factor Alpha (TNFα) and acts by blocking the binding of this cytokine to its receptors. Infliximab also induces apoptosis of TNFα expressing T-lymphocytes.

View a summary of all anti-infliximab antibodies.

Product Form
Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Infliximab
Affinity
The monovalent intrinsic affinity of this antibody in an unconjugated format was measured as KD=0.7 nM by real time, label-free molecular interaction analysis on immobilized infliximab.
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Remicade® is a registered trademark of Janssen Biotech Inc. Inflectra® is a registered trademark of Hospira, a Pfizer Company. RENFLEXIS™ is a trademark of Merck Sharp & Dohme Corp.

This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a detection reagent in a bridging ELISA for PK assay development together with HCA214 or HCA212 as the capture reagent.
Protocol: PK bridging ELISA to measure total drug

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Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
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Description Human anti Infliximab
Human anti Infliximab HCA214 E 0.1 mg loader
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Description Human anti Infliximab
Recombinant Human TNF Alpha PHP051 E FN WB 50 µg loader
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Description Recombinant Human TNF Alpha

References for Infliximab antibody

  1. Kashiwagi, N. et al. () Method for measuring anti-drug antibody
    US Patent Application US2017315118A1

Synonyms
REMICADE®
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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