Infliximab antibody | AbD18391

Human anti Infliximab (Drug/Target Complex)

Product Type
Monoclonal Antibody
Clone
AbD18391
Isotype
Fab antibody
Specificity
Infliximab
Region
DRUG/TARGET COMPLEX

Product Code Applications Pack Size List Price Your Price Qty
New TZA009
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Human Anti-Infliximab (Drug/Target Complex) Antibody, clone AbD18391 specifically recognizes the antibody drug infliximab when in complex with its target, TNFα (cytokine “tumour necrosis factor-alpha”). At defined experimental levels, the antibody does not recognize free infliximab nor unbound recombinant human TNFα. However, as levels greatly increase, detection is noted. It can be used to measure levels of infliximab in patient samples.

Anti-Infliximab monovalent Fab antibody TZA009 has a SpyTag incorporated for conversion by the user into alternative antibody formats using any of the SpyCatchers available in our catalog.

View a summary of all SpyCatcher products

Anti-Infliximab monovalent Fab antibody TZA009 can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free infliximab captured via immobilized TNFα.

Infliximab (Remicade) is a chimeric IgG1/kappa antibody. It has been approved for the treatment of psoriasis, Crohn’s Disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis and ulcerative colitis. Infliximab acts by blocking the binding of TNFα to TNF receptors. Infliximab also causes programmed cell death of TNFα-expressing activated T lymphocytes.

View a summary of all anti-infliximab antibodies

Product Form
A monovalent human recombinant Fab (lambda light chain) in the Fab-F-Spy2-H format selected from the HuCAL® phage display library and expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Infliximab/TNFα complex
Affinity
The monovalent intrinsic affinity of AbD18391 was measured as KD = 16 nM by real time, label free molecular interaction analysis on immobilized biotinylated infliximab complexed with human TNFα.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Remicade is a trademark of Janssen Biotech, Inc.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD18391 can be used as detection antibody for infliximab captured on immoblized TNFα in a PK bridging ELISA.

Protocol: PK bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
List Price Your Price
loader
Description SpyTag3 Peptide

Synonyms
Remicade
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Infliximab specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"