Infliximab antibody | AbD18391





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E ResetHuman anti Infliximab (Drug/Target Complex):HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD18391
- Isotype
- Fab antibody
- Specificity
- Infliximab
- Region
- DRUG/TARGET COMPLEX
Human Anti-Infliximab (Drug/Target Complex) Antibody, clone AbD18391 specifically recognizes the antibody drug infliximab when in complex with its target, TNFα (cytokine “tumour necrosis factor-alpha”). At defined experimental levels, the antibody does not recognize free infliximab nor unbound recombinant human TNFα. However, as levels greatly increase, detection is noted. It can be used to measure levels of infliximab in patient samples. This TrailBlazer Antibody is available in two different formats, monovalent Fab unconjugated (TZA009) and bivalent Fab conjugated to HRP (TZA009P). The monovalent recombinant Fab antibody has a SpyTag incorporated, which is used to create the bivalent format by coupling two SpyTagged Fab antibodies to an HRP conjugated BiSpyCatcher2 (TZC002P). Either format can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free infliximab captured via immobilized TNFα. The antibody is tagged with a SpyTag2 at the C-terminus of the Fab antibody heavy chain, enabling the user to couple it to any of the SpyCatchers available in our catalog for conversion to alternative formats in less than one hour. View a summary of all SpyCatcher products Infliximab (Remicade) is a chimeric IgG1/kappa antibody. It has been approved for the treatment of psoriasis, Crohn’s Disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis and ulcerative colitis. Infliximab acts by blocking the binding of TNFα to TNF receptors. Infliximab also causes programmed cell death of TNFα-expressing activated T lymphocytes. View a summary of all anti-infliximab antibodies |
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- Product Form
- A bivalent human recombinant Fab (lambda light chain) generated by coupling of the monovalent SpyTagged Fab TZA009 with a HRP conjugated BiSpyCatcher version 2 (TZC002P). The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Infliximab/TNFα complex
- Affinity
- The monovalent intrinsic affinity of AbD18391 was measured as KD = 16 nM by real time, label free molecular interaction analysis on immobilized biotinylated infliximab complexed with human TNFα.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Remicade is a trademark of Janssen Biotech, Inc. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
Application Name | Verified | Min Dilution | Max Dilution |
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ELISA | ![]() |
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD18391pap can be used in a direct ELISA to detect the infliximab/TNFα complex or as the detection antibody for infliximab captured on immoblized TNFα in a PK antigen capture ELISA.
Protocol: PK antigen capture ELISA to measure bound drug exclusively.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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Description | Hispec Assay Diluent | ||||||
Human anti Infliximab | HCA212 | E | 0.1 mg |
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Description | Human anti Infliximab | ||||||
Human anti Infliximab | HCA213 | E | 0.1 mg |
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Description | Human anti Infliximab | ||||||
Human anti Infliximab | HCA214 | E | 0.1 mg |
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Description | Human anti Infliximab | ||||||
Human anti Infliximab | HCA215 | E | 0.1 mg |
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Description | Human anti Infliximab | ||||||
Human anti Infliximab | HCA216 | E | 0.1 mg |
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Description | Human anti Infliximab | ||||||
Human anti Infliximab | HCA233 | E | 0.1 mg |
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Description | Human anti Infliximab | ||||||
Human anti Infliximab | HCA234 | E | 0.1 mg |
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Description | Human anti Infliximab | ||||||
Human anti Infliximab (Drug/Target Complex) | TZA009 | E | 0.1 mg |
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Description | Human anti Infliximab (Drug/Target Complex) |
- Synonyms
- Remicade
TZA009P

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