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Infliximab antibody | AbD18391

Human anti Infliximab (Drug/Target Complex):HRP

Product Type
Monoclonal Antibody
Clone
AbD18391
Isotype
Fab antibody
Specificity
Infliximab
Region
DRUG/TARGET COMPLEX

Product Code Applications Pack Size List Price Your Price Qty
New TZA009P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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Human Anti-Infliximab (Drug/Target Complex) Antibody, clone AbD18391 specifically recognizes the antibody drug infliximab when in complex with its target, TNFα (cytokine “tumour necrosis factor-alpha”). At defined experimental levels, the antibody does not recognize free infliximab nor unbound recombinant human TNFα. However, as levels greatly increase, detection is noted. It can be used to measure levels of infliximab in patient samples.

This TrailBlazer Antibody is available in two different formats, monovalent Fab unconjugated (TZA009) and bivalent Fab conjugated to HRP (TZA009P). The monovalent recombinant Fab antibody has a SpyTag incorporated, which is used to create the bivalent format by coupling two SpyTagged Fab antibodies to an HRP conjugated BiSpyCatcher2 (TZC002P). Either format can be used to develop a pharmacokinetic (PK) antigen capture assay to measure free infliximab captured via immobilized TNFα.

The antibody is tagged with a SpyTag2 at the C-terminus of the Fab antibody heavy chain, enabling the user to couple it to any of the SpyCatchers available in our catalog for conversion to alternative formats in less than one hour.

View a summary of all SpyCatcher products

Infliximab (Remicade) is a chimeric IgG1/kappa antibody. It has been approved for the treatment of psoriasis, Crohn’s Disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis and ulcerative colitis. Infliximab acts by blocking the binding of TNFα to TNF receptors. Infliximab also causes programmed cell death of TNFα-expressing activated T lymphocytes.

View a summary of all anti-infliximab antibodies

Product Form
A bivalent human recombinant Fab (lambda light chain) generated by coupling of the monovalent SpyTagged Fab TZA009 with a HRP conjugated BiSpyCatcher version 2 (TZC002P). The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Infliximab/TNFα complex
Affinity
The monovalent intrinsic affinity of AbD18391 was measured as KD = 16 nM by real time, label free molecular interaction analysis on immobilized biotinylated infliximab complexed with human TNFα.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Remicade is a trademark of Janssen Biotech, Inc.

This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD18391pap can be used in a direct ELISA to detect the infliximab/TNFα complex or as the detection antibody for infliximab captured on immoblized TNFα in a PK antigen capture ELISA.

Protocol: PK antigen capture ELISA to measure bound drug exclusively.

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Synonyms
Remicade
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

TZA009P

165711

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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