ZEB1 antibody | OTI8E2

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Mouse anti ZEB1

Product Type
PrecisionAb Monoclonal
Clone
OTI8E2
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00192
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human ZEB1 antibody recognizes ZEB1, also known as delta-crystallin enhancer binding factor 1, negative regulator of IL2, posterior polymorphous corneal dystrophy 3, transcription factor 8 (represses interleukin 2 expression), zinc finger E-box-binding homeobox 1 and zinc finger homeodomain enhancer-binding protein.

ZEB1 encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in ZEB1 have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described (provided by RefSeq, Mar 2010).

Mouse anti Human ZEB1 antibody detects a band of 200 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ZEB1 detects a band of approximately 200 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 1-350 of human ZEB1 (NP_110378) produced in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P37275 Related reagents
Entrez Gene
ZEB1 Related reagents
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0003714 transcription corepressor activity
GO:0005737 cytoplasm
GO:0006955 immune response
GO:0008270 zinc ion binding
GO:0008283 cell proliferation
GO:0016564 transcription repressor activity
GO:0043565 sequence-specific DNA binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ZEB1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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