UCHL3 antibody | AB04/3F5

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Mouse anti UCHL3

Product Type
PrecisionAb Monoclonal
Clone
AB04/3F5
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00932
Datasheet
WB
SDS
100 µl loader

Mouse anti UCHL3 antibody recognizes ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as Ubiquitin thioesterase L3.

Ubiquitin C-terminal hydrolases are deubiquitinating enzymes involved with processing pro-ubiquitin gene products and catalyzing removal of small molecules from ubiquitin (Han et al. 2018). Regulation of the major pathway for repair of DNA double-strand breaks is achieved in part by UCHL3, maintaining genome integrity (Nishi et al. 2018). UCHL3 is phosphorylated and activated by ATM in response to DNA damage (Luo et al. 2016), and plays a role in DNA repair by ubiquitylating TDP1. Disruption of TDP1 can cause neurological disease and resistance to chemotherapy in cancer (Liao et al. 2018). A role of UCHL3 has been suggested in the response to cancer therapy, with high expression rendering breast cancer cells resistant to radiation and chemotherapy, and low expression sensitizing cells to these treatments (Luo et al. 2016).

Product Details

Target Species
Human
Western Blotting
Mouse anti UCHL3 detects a band of approximately 25 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-230 of human UCHL3
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P15374
Entrez Gene
UCHL3
GO Terms
GO:0008234 cysteine-type peptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0005737 cytoplasm
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0043130 ubiquitin binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of UCHL3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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