Tubulin Beta 3 antibody | OTI5H2

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Mouse anti Tubulin Beta 3

Mouse anti human tubulin beta 3 antibody recognizes class III beta-tubulin, also known as TUBB4 or fibrosis of extraocular muscles, congenital, 3. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI5H2
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00397 WB 100 µl
Mouse anti Human tubulin Beta 3 antibody recognizes tubulin beta 3, also known as TUBB3, class III beta-tubulin and tubulin beta-III.

The TUBB3 gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in TUBB3 are the cause of congenital fibrosis of the extra-ocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of TUBB3 is found on chromosome 6 (provided by RefSeq, Oct 2010).

Mouse anti Human tubulin beta 3 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti tubulin beta 3 detects a band of approximately 55 kDa in mouse and human brain tissue
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 1-150 of human tubulin beta 3 (NP_006077) produced in E.coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q13509 Related reagents
Entrez Gene
TUBB3 Related reagents
GO Terms
GO:0003924 GTPase activity
GO:0005198 structural molecule activity
GO:0005525 GTP binding
GO:0005737 cytoplasm
GO:0005874 microtubule
GO:0007018 microtubule-based movement
GO:0007411 axon guidance
GO:0051084 'de novo' posttranslational protein folding
GO:0051258 protein polymerization
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Tubulin Beta 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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