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Tubulin Beta 3 antibody | OTI5H2

Mouse anti Tubulin Beta 3

Product Type
PrecisionAb Monoclonal
Clone
OTI5H2
Isotype
IgG1
Format
Purified
Specificity
Tubulin Beta 3

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New VMA00397
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human tubulin Beta 3 antibody, clone OTI5H2 recognizes tubulin beta 3, also known as TUBB3, class III beta-tubulin and tubulin beta-III.

The TUBB3 gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in TUBB3 are the cause of congenital fibrosis of the extra-ocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of TUBB3 is found on chromosome 6 (provided by RefSeq, Oct 2010).

Mouse anti Human tubulin beta 3 antibody, clone OTI5H2 detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti tubulin beta 3 detects a band of approximately 55 kDa in mouse and human brain tissue.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 1-150 of human tubulin beta 3 (NP_006077) produced in E.coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q13509
Entrez Gene
TUBB3
GO Terms
GO:0003924 GTPase activity
GO:0005198 structural molecule activity
GO:0005525 GTP binding
GO:0005737 cytoplasm
GO:0005874 microtubule
GO:0007018 microtubule-based movement
GO:0007411 axon guidance
GO:0051084 'de novo' posttranslational protein folding
GO:0051258 protein polymerization

VMA00397

160322

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