Tubulin Alpha 1A Chain antibody | OTI2C8

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Anti Tubulin Alpha 1A Chain Antibody, clone OTI2C8 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Tubulin Alpha 1A Chain Antibody, clone OTI2C8 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Tubulin Alpha 1A Chain Antibody, clone OTI2C8 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti Tubulin Alpha 1A Chain Antibody, clone OTI2C8 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human tubulin alpha 1A chain antibody, clone OTI2C8 (**VMA00173**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 13 second exposure. Arrow points to tubulin alpha 1A chain (molecular weight 50 kDa).

Anti Tubulin Alpha 1A Chain Antibody, clone OTI2C8 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Tubulin Alpha 1A Chain (1 & 2) or a scrambled siRNA (-) probed with Mouse anti Tubulin Alpha 1A Chain antibody (VMA00173) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-actin primary antibody (12004163) and visualized on the ChemiDoc MP with an 120 second (chemiluminescent channel) and 60 second (rhodamine channel) exposure.

Anti Tubulin Alpha 1A Chain Antibody, clone OTI2C8 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of Tubulin Alpha 1A Chain from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Tubulin Alpha 1A Chain (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the actin signal.

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Mouse anti Tubulin Alpha 1A Chain

Product Type: PrecisionAb Monoclonal
Clone: OTI2C8
Isotype: IgG2b
Format: Purified
Specificity: Tubulin Alpha 1A Chain

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00173 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human tubulin alpha 1A chain antibody, clone OTI2C8 recognizes the tubulin alpha 1A chain, also known as hum-a-tub1, hum-a-tub2, tubulin B-alpha-1, tubulin alpha-1A chain, tubulin alpha-3 chain and tubulin, alpha, brain-specific.

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The TUBA1A gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting shows that the gene expression is predominantly found in morphologically differentiated neurologic cells. TUBA1A is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in TUBA1A cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Jul 2012).

Mouse anti Human tubulin alpha 1A chain antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti tubulin alpha 1A chain detects a band of approximately 50 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
25% Glycerol
Immunogen: Full length recombinant human tubulin alpha 1A chain (NP_006000) produced in HEK293T cells
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q71U36
Entrez Gene: TUBA1A
GO Terms: GO:0000086 G2/M transition of mitotic cell cycle; GO:0003924 GTPase activity; GO:0005829 cytosol; GO:0005198 structural molecule activity; GO:0005525 GTP binding; GO:0005874 microtubule; GO:0007018 microtubule-based movement; GO:0051084 'de novo' posttranslational protein folding; GO:0051258 protein polymerization; View More GO Terms