Tubulin Alpha 1A Chain antibody | OTI2C8

100% Secure


Mouse anti Tubulin Alpha 1A Chain

Product Type
PrecisionAb Monoclonal
Clone
OTI2C8
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00173
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human tubulin alpha 1A chain antibody recognizes the tubulin alpha 1A chain, also known as hum-a-tub1, hum-a-tub2, tubulin B-alpha-1, tubulin alpha-1A chain, tubulin alpha-3 chain and tubulin, alpha, brain-specific.

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The TUBA1A gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. TUBA1A is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in TUBA1A cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Jul 2012).

Mouse anti Human tubulin alpha 1A chain antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti tubulin alpha 1A chain detects a band of approximately 50 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human tubulin alpha 1A chain (NP_006000) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q71U36 Related reagents
Entrez Gene
TUBA1A Related reagents
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0003924 GTPase activity
GO:0005829 cytosol
GO:0005198 structural molecule activity
GO:0005525 GTP binding
GO:0005874 microtubule
GO:0007018 microtubule-based movement
GO:0051084 'de novo' posttranslational protein folding
GO:0051258 protein polymerization
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Tubulin Alpha 1A Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Western Blotting without Trial and Error: Use PrecisionAb Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.