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Troponin T antibody | C03-9H8

Mouse anti Troponin T (Cardiac)

Product Type
PrecisionAb Monoclonal
Clone
C03-9H8
Isotype
IgG2b
Format
Purified
Specificity
Troponin T
Region
(CARDIAC)

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VMA00101
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Mouse anti Human troponin T antibody recognizes troponin T, cardiac muscle, also known as cardiomyopathy dilated 1D (autosomal dominant) and cardiomyopathy hypertrophic 2.

Encoded by the TNNT2 gene, troponin T is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in TNNT2 have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for TNNT2 undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined (provided by RefSeq, Jul 2008).

Mouse anti Human troponin T antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti troponin T detects a band of approximately 40 kDa in human heart cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified native Troponin T protein from human cardiac tissue.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P45379
Entrez Gene
TNNT2
GO Terms
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005523 tropomyosin binding
GO:0005861 troponin complex
GO:0008016 regulation of heart contraction
GO:0030049 muscle filament sliding
GO:0030172 troponin C binding
GO:0031013 troponin I binding
GO:0032780 negative regulation of ATPase activity
GO:0032781 positive regulation of ATPase activity
GO:0051592 response to calcium ion
GO:0055010 ventricular cardiac muscle tissue morphogenesis

VMA00101

080615

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