TRIM21 antibody | AB01/1G5

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Mouse anti TRIM21

Product Type
PrecisionAb Monoclonal
Clone
AB01/1G5
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00730
Datasheet
WB
SDS
100 µl loader

Mouse anti Human TRIM21 antibody recognizes the E3 ubiquitin-protein ligase TRIM21, also known as RO52, RO/SSA, RING finger protein 81, SS-A and Sicca syndrome antigen A.

In humans, the TRIM family of proteins includes over 100 structurally similar proteins with a broad range of cellular roles. TRIM21 is an Fc receptor involved in the immune response within cells, binding to antibodies that are carried by pathogens into the cytosol, and activating protective inflammatory signaling and antiviral responses. (Foss et al. 2015). TRIM21 is associated with autoimmune diseases including systemic lupus erythematosus (SLE) and Sjögren syndrome, involving hyperactivation of the type 1 interferon response (Kimura et al. 2017). TRIM21 has protective effects in hepatocellular carcinoma and diffuse large B-cell lymphoma, but conversely, low levels of TRIM21 correlate with prolonged overall survival and disease-free survival in pancreatic cancer. These differences may reflect the differences in downstream targets of TRIM21 involved with different types of cancer (Nguyen and Irby 2017).

Product Details

Target Species
Human
Western Blotting
Mouse anti TRIM21 detects a band of approximately 52 kDa in HEK293 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-475 of human TRIM21
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P19474
Entrez Gene
TRIM21
GO Terms
GO:0000209 protein polyubiquitination
GO:0005515 protein binding
GO:0000932 cytoplasmic mRNA processing body
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004842 ubiquitin-protein ligase activity
GO:0005634 nucleus
GO:0006513 protein monoubiquitination
GO:0007049 cell cycle
GO:0008270 zinc ion binding
GO:0031648 protein destabilization
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0045787 positive regulation of cell cycle
GO:0051865 protein autoubiquitination
GO:0070206 protein trimerization
GO:0090086 negative regulation of protein deubiquitination
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of TRIM21 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml