TFEB antibody | AB01/2D12

Mouse anti TFEB

Product Type
PrecisionAb Monoclonal
Clone
AB01/2D12
Isotype
IgG1
Format
Purified
Specificity
TFEB

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VMA00972
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Mouse anti TFEB antibody recognizes transcription factor EB, also known as bHLHe35. TFEB1 is a transcription factor which plays an important role in cellular processes including lysosomal biogenesis, cellular energy metabolism, lipid catabolism and autophagy. TFEB activity and localization is regulated by mTOR at the surface of lysosomes (Napolitano and Ballabio, 2016). When activated, TFEB controls the expression of genes that regulate biogenesis of lysosomes and autophagosomes (Nnah et al. 2019). Evidence has also shown that TFEB becomes activated in response to mitochondrial and ER stress, suggesting a role in cellular adaptation to stress (Raben and Puertollano 2016). TFEB is upregulated in a range of cancer types including non-Hodgkin's lymphoma. In late-stage colorectal cancer, it is been suggested that TFEB could regulate autophagy and promote tumor growth and metastasis (Liang et al. 2018). PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti TFEB detects a band of approximately 62 kDa in Raji cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-476 of human TFEB
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P19484
Entrez Gene
TFEB
GO Terms
GO:0001892 embryonic placenta development
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005737 cytoplasm
GO:0006959 humoral immune response
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0030528 transcription regulator activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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