Browsing Modes for Business Users

STAT3 antibody | 3B5

ISO 9001 Quality Assurance Manufacturing | Print
KO Validated
Anti STAT3 Antibody, clone 3B5 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti STAT3 Antibody, clone 3B5 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti STAT3 Antibody, clone 3B5 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti STAT3 Antibody, clone 3B5 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with STAT3 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 37 second exposure. Arrow points to STAT3 (molecular weight 84 kDa)
Anti STAT3 Antibody, clone 3B5 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of STAT3 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti STAT3 antibody (VMA00242) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 79 second and (B) 30 second exposure.
Anti STAT3 Antibody, clone 3B5 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of STAT3 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Mouse anti STAT3

Product Type
PrecisionAb Monoclonal
Clone
3B5
Isotype
IgG1
Format
Purified
Specificity
STAT3

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
VMA00242
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader

Mouse anti Human STAT3 antibody recognizes signal transducer and activator of transcription 3, also known as DNA-binding protein APRF, acute-phase response factor and signal transducer and activator of transcription 3.

Encoded by STAT3 gene, signal transducer and activator of transcription 3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described (provided by RefSeq, Jul 2008).

Mouse anti Human STAT3 antibody detects a band of 84 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti STAT3 detects a band of approximately 84 kDa in LNCaP cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified recombinant fragment of human STAT3 expressed in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P40763
Entrez Gene
STAT3
GO Terms
GO:0005886 plasma membrane
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0005509 calcium ion binding
GO:0001659 temperature homeostasis
GO:0001754 eye photoreceptor cell differentiation
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006606 protein import into nucleus
GO:0005829 cytosol
GO:0004871 signal transducer activity
GO:0005634 nucleus
GO:0006928 cellular component movement
GO:0008134 transcription factor binding
GO:0016563 transcription activator activity
GO:0019901 protein kinase binding
GO:0019953 sexual reproduction
GO:0032355 response to estradiol stimulus
GO:0042593 glucose homeostasis
GO:0042755 eating behavior
GO:0044419 interspecies interaction between organisms
GO:0046983 protein dimerization activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway
GO:0070102 interleukin-6-mediated signaling pathway

VMA00242

151111

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with STAT3 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
Please note