SRC antibody | 4F10B5
Rabbit anti SRC antibody recognizes proto-oncogene tyrosine-protein kinase Src, also known as Src1, p60-Src and c-Src.
c-Src is a ubiquitously-expressed cytoplasmic tyrosine kinase, important for regulating diverse cellular functions (Honda et al. 2018). c-Src was the first oncogene to be identified, over-expressed and abnormally activated in cancer cells, and is the cellular counterpart of v-Src, a cancer-causing gene found in the Rous sarcoma virus (Hunter and Sefton, 1980). Src functions as a complex molecular switch, which can self-modulate its activity in response to specific signals. This is achieved by a reciprocally regulatory mechanism involving Tyr416 and Tyr527, changing the tertiary structure of Src and controlling access to its active site (Knighton et al. 1991).
c-Src is a ubiquitously-expressed cytoplasmic tyrosine kinase, important for regulating diverse cellular functions (Honda et al. 2018). c-Src was the first oncogene to be identified, over-expressed and abnormally activated in cancer cells, and is the cellular counterpart of v-Src, a cancer-causing gene found in the Rous sarcoma virus (Hunter and Sefton, 1980). Src functions as a complex molecular switch, which can self-modulate its activity in response to specific signals. This is achieved by a reciprocally regulatory mechanism involving Tyr416 and Tyr527, changing the tertiary structure of Src and controlling access to its active site (Knighton et al. 1991).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Recombinant protein of human SRC
Storage Information
- Storage
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P12931
- Entrez Gene
- SRC
- GO Terms
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0005070 SH3/SH2 adaptor activity
- GO:0005178 integrin binding
- GO:0005524 ATP binding
- GO:0005901 caveola
- GO:0006468 protein phosphorylation
- GO:0007172 signal complex assembly
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0007243 intracellular protein kinase cascade
- GO:0007265 Ras protein signal transduction
- GO:0007411 axon guidance
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0016044 cellular membrane organization
- GO:0030168 platelet activation
- GO:0031295 T cell costimulation
- GO:0033625 positive regulation of integrin activation
- GO:0034329 cell junction assembly
- GO:0042169 SH2 domain binding
- GO:0043114 regulation of vascular permeability
- GO:0044419 interspecies interaction between organisms
- GO:0045124 regulation of bone resorption
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050900 leukocyte migration
- GO:0070555 response to interleukin-1
- Regulatory
- For research purposes only
Applications of SRC antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
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