SRC antibody | 4F10B5

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Rabbit anti SRC

Product Type
Monoclonal Antibody
Clone
4F10B5
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6142
Datasheet
WB
MSDS
50 µl loader

Rabbit anti SRC antibody recognizes proto-oncogene tyrosine-protein kinase Src, also known as Src1, p60-Src and c-Src.

c-Src is a ubiquitously-expressed cytoplasmic tyrosine kinase, important for regulating diverse cellular functions (Honda et al. 2018). c-Src was the first oncogene to be identified, over-expressed and abnormally activated in cancer cells, and is the cellular counterpart of v-Src, a cancer-causing gene found in the Rous sarcoma virus (Hunter and Sefton, 1980). Src functions as a complex molecular switch, which can self-modulate its activity in response to specific signals. This is achieved by a reciprocally regulatory mechanism involving Tyr416 and Tyr527, changing the tertiary structure of Src and controlling access to its active site (Knighton et al. 1991).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant protein of human SRC

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P12931
Entrez Gene
SRC
GO Terms
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005070 SH3/SH2 adaptor activity
GO:0005178 integrin binding
GO:0005524 ATP binding
GO:0005901 caveola
GO:0006468 protein phosphorylation
GO:0007172 signal complex assembly
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007243 intracellular protein kinase cascade
GO:0007265 Ras protein signal transduction
GO:0007411 axon guidance
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0016044 cellular membrane organization
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0033625 positive regulation of integrin activation
GO:0034329 cell junction assembly
GO:0042169 SH2 domain binding
GO:0043114 regulation of vascular permeability
GO:0044419 interspecies interaction between organisms
GO:0045124 regulation of bone resorption
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050900 leukocyte migration
GO:0070555 response to interleukin-1
Regulatory
For research purposes only

Applications of SRC antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg