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Spastin antibody | RM346

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Anti Spastin Antibody, clone RM346 thumbnail image 1 Anti Spastin Antibody, clone RM346 thumbnail image 2 Anti Spastin Antibody, clone RM346 thumbnail image 3
Anti Spastin Antibody, clone RM346 gallery image 1
Western blot analysis of HeLa cells, transfected with control siRNA (1) or spastin siRNA and probed with Rabbit anti spastin (1/500, MCA6350). Arrow points to spastin.
Anti Spastin Antibody, clone RM346 gallery image 2
Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using Rabbit anti spastin (1/1000, MCA6350).
Anti Spastin Antibody, clone RM346 gallery image 3
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using Rabbit anti spastin (1/1000, MCA6350).

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Rabbit anti Spastin

Product Type
Monoclonal Antibody
Clone
RM346
Isotype
IgG
Specificity
Spastin

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Product Code Applications Pack Size List Price Your Price Qty
MCA6350
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Rabbit anti spastin antibody recognizes spastin, also known as spastic paraplegia 4 protein and SPAST.

Spastin is an ATPase which uses energy from ATP hydrolysis to promote microtubule severing. The two spastin isoforms are ubiquitously-expressed but have different subcellular locations (primarily the cytoplasm and endoplasmic reticulum). They appear to work together to promote fission of fubulues from the early sorting endosome (Allison et al. 2019). In addition to severing microtubules, spastin promotes regrowth of microtubules in an ATP-independent way, revealing its function as a dual-microtubule regulator (Kuo et al. 2019). Spastin is involved with remodeling of membrane structures, relevant for neurite branching, axonal growth, midbody abscission, and endosome tubulation (Papadopoulos et al. 2015). Mutations in spastin are a common cause of Hereditary Spastic Paraplegia (HSP), a group of diseases characterized by spasticity and gait disorders (Arribat et al. 2020).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the N-terminus of human spastin
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/500 1/1000
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
Q9UBP0
Entrez Gene
SPAST
GO Terms
GO:0001578 microtubule bundle formation
GO:0016021 integral to membrane
GO:0005783 endoplasmic reticulum
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005768 endosome
GO:0005815 microtubule organizing center
GO:0005819 spindle
GO:0005874 microtubule
GO:0006888 ER to Golgi vesicle-mediated transport
GO:0007049 cell cycle
GO:0007109 cytokinesis, completion of separation
GO:0007399 nervous system development
GO:0008017 microtubule binding
GO:0008219 cell death
GO:0051013 microtubule severing
GO:0008568 microtubule-severing ATPase activity
GO:0030154 cell differentiation
GO:0034214 protein hexamerization
GO:0051260 protein homooligomerization
GO:0043014 alpha-tubulin binding
GO:0048471 perinuclear region of cytoplasm
GO:0048487 beta-tubulin binding

MCA6350

157613

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