RPS2 antibody | EF05/3G8

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Mouse anti RPS2

Product Type
PrecisionAb Monoclonal
Clone
EF05/3G8
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00916
Datasheet
WB
SDS
100 µl loader

Mouse anti RPS2 antibody recognizes 40S ribosomal protein S2, also known as Protein LLRep3 and small ribosomal subunit protein uS5.

RPS2 encodes a protein that forms an extraribosomal complex with PRMT1, conserved from fission yeast to humans, and may interact with other proteins to fulfil key roles beyond the ribosome (Dionne et al. 2019). Regulatory functions of RPS2 include controlling the binding affinity between the 40S ribosome component and mRNA, and transport of 40S precursors from the nucleus to the cytoplasm (Minakhina et al. 2016). Elevated RPS2 mRNA is associated with increased cellular proliferation, and RPS2 is upregulated in a range of cancer types. RPS2 has been suggested as a potential therapeutic target in colorectal cancer because downregulation of RPS2 diminishes the viability of CRC cell lines (Grade et al. 2011).

Product Details

Target Species
Human
Western Blotting
Mouse anti RPS2 detects a band of approximately 31 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-293 of human RPS2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P15880
Entrez Gene
RPS2
GO Terms
GO:0022627 cytosolic small ribosomal subunit
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0005654 nucleoplasm
GO:0006414 translational elongation
GO:0006415 translational termination
GO:0019083 viral transcription
GO:0031018 endocrine pancreas development
GO:0048602 fibroblast growth factor 1 binding
GO:0048604 fibroblast growth factor 3 binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of RPS2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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