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RIPK1 antibody | AB01/3C7

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Anti RIPK1 Antibody, clone AB01/3C7 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti RIPK1 Antibody, clone AB01/3C7 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti RIPK1 Antibody, clone AB01/3C7 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Mouse anti Human RIPK1 antibody (VMA00421) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemicDoc MP with 21 second exposure. Arrows point to RIPK1 (molecular weight 78 and 38 kDa).
Anti RIPK1 Antibody, clone AB01/3C7 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of RIPK1 from HEK293 whole cell lysate. The Input lane is 10% input; the RIPK1-IP lane is the immunoprecipitation with the RIPK1 antibody (2.5 µg, VMA00421); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using RIPK1 antibody (1/1000, VMA00421) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10000, 1705047) and visualized on the ChemiDoc MP with 36.5 second expsoure. Arrow points to RIPK1.

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WB IP

Mouse anti RIPK1

Product Type
PrecisionAb Monoclonal
Clone
AB01/3C7
Isotype
IgG1
Format
Purified
Specificity
RIPK1

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Product Code Applications Pack Size List Price Your Price Qty
VMA00421
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Mouse anti Human RIPK1 antibody recognizes the RIPK1, also known as receptor-interacting protein 1 (RIP1), receptor-interacting serine/threonine-protein kinase 1 or serine/threonine-protein kinase RIP.

RIPK1 transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Mouse anti Human RIPK1 antibody detects a bands of 78 and 38 kDa. The full length protein is approximately 78 kDa. The 38 kDa band is believed to be a fragment of the RIPK1 protein. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti RIPK1 detects a bands of approximately 78 and 38 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E.coli-derived recombinant protein corresponding to aa 1-671 of human RIPK1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
Q13546
Entrez Gene
RIPK1
GO Terms
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005123 death receptor binding
GO:0005524 ATP binding
GO:0006919 activation of caspase activity
GO:0007257 activation of JUN kinase activity
GO:0008063 Toll signaling pathway
GO:0008624 induction of apoptosis by extracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0010008 endosome membrane
GO:0031264 death-inducing signaling complex
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0034612 response to tumor necrosis factor
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043235 receptor complex
GO:0045087 innate immune response
GO:0045768 positive regulation of anti-apoptosis
GO:0046777 protein autophosphorylation
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0060555 induction of necroptosis by extracellular signals
GO:0070513 death domain binding
GO:0070926 regulation of ATP:ADP antiporter activity
GO:0080010 regulation of oxygen and reactive oxygen species metabolic process

VMA00421

160303

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