Find out more about A.I.den - our SearchBot

RAF-1 antibody | 4G4

ISO 9001 Quality Assurance Manufacturing | Print
siRNA Validated
Anti RAF-1 Antibody, clone 4G4 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti RAF-1 Antibody, clone 4G4 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti RAF-1 Antibody, clone 4G4 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti RAF-1 Antibody, clone 4G4 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with RAF-1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 120 second exposure. Arrow points to RAF-1 (molecular weight 73 kDa).
Anti RAF-1 Antibody, clone 4G4 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against RAF-1 (1 & 2) or a scrambled siRNA (-) probed with Mouse anti RAF-1 antibody (VMA00124) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 1705047) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 300 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.
Anti RAF-1 Antibody, clone 4G4 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of RAF-1 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against RAF-1 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Filter by Application:

WB

Mouse anti RAF-1

Product Type
PrecisionAb Monoclonal
Clone
4G4
Isotype
IgG1
Format
Purified
Specificity
RAF-1

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
VMA00124
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
List Price Your Price
loader

Mouse anti Human RAF-1 antibody recognizes RAF-1, also known as C-Raf proto-oncogene, serine/threonine kinase, Oncogene RAF1, RAF proto-oncogene serine/threonine-protein kinase, proto-oncogene c-RAF, raf proto-oncogene serine/threonine protein kinase or v-raf-1 murine leukemia viral oncogene homolog 1.

RAF-1 is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF-1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in RAF-1 are associated with Noonan syndrome 5 and LEOPARD syndrome 2 (provided by RefSeq, Jul 2008).

Mouse anti Human RAF-1 antibody detects a band of 73 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti RAF-1 detects a band of approximately 73 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human RAF-1 expressed in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

Further Reading

  1. O'Neill ,E. & Kolch, W. (2005) Taming the Hippo: Raf-1 controls apoptosis by suppressing MST2/Hippo.
    Cell Cycle. 4(3): 365-7.
  2. Morrison, D.K. & Cutler, R.E. (1997) The complexity of Raf-1 regulation.
    Curr Opin Cell Biol. 9(2):174-9

Synonyms
Proto-Oncogene c-RAF
UniProt
P04049
Entrez Gene
RAF1
GO Terms
GO:0005886 plasma membrane
GO:0000186 activation of MAPKK activity
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005057 receptor signaling protein activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005741 mitochondrial outer membrane
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007268 synaptic transmission
GO:0007411 axon guidance
GO:0008283 cell proliferation
GO:0008285 negative regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0030168 platelet activation
GO:0048011 nerve growth factor receptor signaling pathway

VMA00124

120615

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with RAF-1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products