PSMC1 antibody | EF02/2E2

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Mouse anti PSMC1

Product Type
PrecisionAb Monoclonal
Clone
EF02/2E2
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00913
Datasheet
WB
SDS
100 µl loader

Mouse anti PSMC1 antibody recognizes 26S proteasome regulatory subunit 4, also known as PRS4 and P26s4.

PSMC1 is a ubiquitously-expressed ATPase which belongs to the triple-A family and plays an essential role in the 19S proteasome, essential for correct function of all cells. Incorrect function of the 26S proteasome, leading to an inability to degrade proteins, has been implicated in neurodegenerative diseases (Czub et al. 2016). A role of PSMC1 in the pathogenesis of Parkinson's disease has been suggested, but appears unlikely (Gomez-Garre et al. 2012). PSMC1 exists in a complex with PSMD2 and PSMD5, and may act as a reservoir to protect cells from harmful effects of suppression of the essential gene encoding PSMC2 (Nijhawan et al. 2012).

Product Details

Target Species
Human
Western Blotting
Mouse anti PSMC1 detects a band of approximately 57 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-440 of human PSMC1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P62191
Entrez Gene
PSMC1
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000084 S phase of mitotic cell cycle
GO:0005515 protein binding
GO:0000216 M/G1 transition of mitotic cell cycle
GO:0000502 proteasome complex
GO:0006915 apoptosis
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006521 regulation of cellular amino acid metabolic process
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
GO:0016887 ATPase activity
GO:0016032 viral reproduction
GO:0016071 mRNA metabolic process
GO:0042981 regulation of apoptosis
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PSMC1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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