PI-3 Kinase C2 Subunit Alpha antibody | OTI1B8

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Mouse anti PI-3 Kinase C2 Subunit Alpha

PI-3 kinase C2 subunit alpha antibody recognizes phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, also known as C2-containing phosphatidylinositol kinase or PI3K-C2-alpha. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1B8
Isotype
IgG2b
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00263 WB 100 µl
Mouse anti Human PI-3 kinase C2 subunit alpha antibody recognizes the phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, also known as C2-containing phosphatidylinositol kinase, PI3K-C2-alpha, PI3K-C2alpha, phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha, phosphoinositide 3-kinase-C2-alpha, phosphoinositide-3-kinase, class 2, alpha polypeptide and ptdIns-3-kinase C2 subunit alpha.

The protein encoded by PIK3C2A gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. (provided by RefSeq, Jul 2008).

Mouse anti Human PI-3 kinase C2 subunit alpha antibody detects a band of 191 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PI-3 Kinase C2 subunit alpha detects a band of approximately 191 kDa in C6 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 230-560 of human PI-3 C2 subunit alpha (NP_002636) produced in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
O00443 Related reagents
Entrez Gene
PIK3C2A Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005794 Golgi apparatus
GO:0005942 phosphatidylinositol 3-kinase complex
GO:0006661 phosphatidylinositol biosynthetic process
GO:0007154 cell communication
GO:0016303 1-phosphatidylinositol-3-kinase activity
GO:0035005 phosphatidylinositol-4-phosphate 3-kinase activity
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030136 clathrin-coated vesicle
GO:0035091 phosphatidylinositol binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PI-3 Kinase C2 Subunit Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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