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NFkB p65 antibody | RM273

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Anti NFkB p65 Antibody, clone RM273 thumbnail image 1 Anti NFkB p65 Antibody, clone RM273 thumbnail image 2
Anti NFkB p65 Antibody, clone RM273 gallery image 1
Western blot analysis of MCF-7 cell lysates probed with Rabbit anti NFkB p65 (1/500, MCA6316). Arrow points to NFkB p65.
Anti NFkB p65 Antibody, clone RM273 gallery image 2
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Rabbit anti NFkB p65 (MCA6316) at a 1/1250 dilution.

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WB

Rabbit anti NFkB p65

Product Type
Monoclonal Antibody
Clone
RM273
Isotype
IgG
Specificity
NFkB p65

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Product Code Applications Pack Size List Price Your Price Qty
MCA6316
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P WB 0.1 ml loader
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loader

Rabbit anti Human NFkB p65 antibody recognizes transcription factor p65, also known as RelA, NFKB3. Members of the NFkB family of transcription factors are critical regulators of immunity and inflammation in response to infection and injury. The most abundant form of NFkB comprises a heterodimer of the NFkB proteins p65 and p50. Under non-inflammatory conditions p65:p50 is held in the cytoplasm by inhibitor kappa b alpha (IKBa). When the cell senses an inflammatory stimulus such as tumor necrosis factor alpha (TNF alpha), IKBa is degraded and the nuclear localization signal (NLS) of p65 released. p65:p50 then enters the nucleus where it activates transcription of target genes, which include IKBa, comprising a negative feedback loop (Giridharan and Srinivasan 2018). Transient NFkB activation is important for a protective immune response, but constant activation underlies many chronic inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease (IBD) (Liu et al. 2017). NFkB is also constitutively activated in many types of cancer and has many pro-tumorigenic functions, particularly in cancers that emerge from sites of chronic inflammation (Hoesel and Schmid 2013).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the area near the C-terminus of NFkB p65
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/500 1/1500
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
Q04206
Entrez Gene
RELA
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003682 chromatin binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005829 cytosol
GO:0005667 transcription factor complex
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0006968 cellular defense response
GO:0008063 Toll signaling pathway
GO:0042802 identical protein binding
GO:0010224 response to UV-B
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0019221 cytokine-mediated signaling pathway
GO:0019901 protein kinase binding
GO:0031293 membrane protein intracellular domain proteolysis
GO:0031625 ubiquitin protein ligase binding
GO:0032582 negative regulation of gene-specific transcription
GO:0033613 activating transcription factor binding
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0042301 phosphate binding
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0047485 protein N-terminus binding
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050727 regulation of inflammatory response
GO:0050852 T cell receptor signaling pathway
GO:0051059 NF-kappaB binding
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051607 defense response to virus
GO:0070431 nucleotide-binding oligomerization domain containing 2 signaling pathway
GO:0070491 repressing transcription factor binding
GO:0070555 response to interleukin-1

MCA6316

155786

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