NFkB p65 antibody | RM273
Rabbit anti Human NFkB p65 antibody recognizes transcription factor p65, also known as RelA, NFKB3. Members of the NFkB family of transcription factors are critical regulators of immunity and inflammation in response to infection and injury. The most abundant form of NFkB comprises a heterodimer of the NFkB proteins p65 and p50. Under non-inflammatory conditions p65:p50 is held in the cytoplasm by inhibitor kappa b alpha (IKBa). When the cell senses an inflammatory stimulus such as tumor necrosis factor alpha (TNF alpha), IKBa is degraded and the nuclear localization signal (NLS) of p65 released. p65:p50 then enters the nucleus where it activates transcription of target genes, which include IKBa, comprising a negative feedback loop (Giridharan and Srinivasan 2018). Transient NFkB activation is important for a protective immune response, but constant activation underlies many chronic inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease (IBD) (Liu et al. 2017). NFkB is also constitutively activated in many types of cancer and has many pro-tumorigenic functions, particularly in cancers that emerge from sites of chronic inflammation (Hoesel and Schmid 2013).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- A peptide corresponding to the area near the C-terminus of NFkB p65
Storage Information
- Storage
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q04206
- Entrez Gene
- RELA
- GO Terms
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003682 chromatin binding
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0005829 cytosol
- GO:0005667 transcription factor complex
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0006968 cellular defense response
- GO:0008063 Toll signaling pathway
- GO:0042802 identical protein binding
- GO:0010224 response to UV-B
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- GO:0019221 cytokine-mediated signaling pathway
- GO:0019901 protein kinase binding
- GO:0031293 membrane protein intracellular domain proteolysis
- GO:0031625 ubiquitin protein ligase binding
- GO:0032582 negative regulation of gene-specific transcription
- GO:0033613 activating transcription factor binding
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0042301 phosphate binding
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0047485 protein N-terminus binding
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050727 regulation of inflammatory response
- GO:0050852 T cell receptor signaling pathway
- GO:0051059 NF-kappaB binding
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051607 defense response to virus
- GO:0070431 nucleotide-binding oligomerization domain containing 2 signaling pathway
- GO:0070491 repressing transcription factor binding
- GO:0070555 response to interleukin-1
- Regulatory
- For research purposes only
Applications of NFkB p65 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/500 | 1/1500 | |
| Western Blotting | 1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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