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NF Kappa B Inhibitor Alpha antibody | OTI1D4

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Anti NF Kappa B Inhibitor Alpha Antibody, clone OTI1D4 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti NF Kappa B Inhibitor Alpha Antibody, clone OTI1D4 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti NF Kappa B Inhibitor Alpha Antibody, clone OTI1D4 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with NF kappa B inhibitor alpha antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 67 second exposure. Arrow points to NF kappa B inhibitor alpha (molecular weight 35 kDa).
Anti NF Kappa B Inhibitor Alpha Antibody, clone OTI1D4 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of NF Kappa B Inhibitor Alpha from MCF-7 whole cell lysate. The Input lane is 10% input; the NF Kappa B Inhibitor Alpha-IP lane is the immunoprecipitation with the NF Kappa B Inhibitor Alpha antibody (2.5 μg, VMA00150); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 μg, MCA928). Western blot analysis was performed using NF Kappa B Inhibitor Alpha antibody (1/1000, VMA00150) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP with 62 second exposure. Arrow points to NF Kappa B Inhibitor Alpha.

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WB

Mouse anti NF Kappa B Inhibitor Alpha

Product Type
PrecisionAb Monoclonal
Clone
OTI1D4
Isotype
IgG1
Format
Purified
Specificity
NF Kappa B Inhibitor Alpha

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Product Code Applications Pack Size List Price Your Price Qty
VMA00150
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SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Mouse anti Human NF kappa B inhibitor alpha antibody recognizes NF kappa B inhibitor alpha, also known as I-kappa-B-alpha, IkappaBalpha, NF-kappa-B inhibitor alpha, ikB-alpha, major histocompatibility complex enhancer-binding protein MAD3 and nuclear factor of kappa light chain gene enhancer in B-cells.

Human NF kappa B inhibitor alpha is a member of the NF kappa B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF kappa B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in the NFKBIA gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease (provided by RefSeq, Aug 2011).

Mouse anti Human Human NF kappa B inhibitor alpha antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti NF kappa B inhibitor alpha detects a band of approximately 35 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human NF kappa B inhibitor alpha (NP_065390) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

UniProt
P25963
Entrez Gene
NFKBIA
GO Terms
GO:0005886 plasma membrane
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006916 anti-apoptosis
GO:0007253 cytoplasmic sequestering of NF-kappaB
GO:0008063 Toll signaling pathway
GO:0008139 nuclear localization sequence binding
GO:0042802 identical protein binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010875 positive regulation of cholesterol efflux
GO:0010888 negative regulation of lipid storage
GO:0031625 ubiquitin protein ligase binding
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0032270 positive regulation of cellular protein metabolic process
GO:0033256 I-kappaB/NF-kappaB complex
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0051059 NF-kappaB binding
GO:0051092 positive regulation of NF-kappaB transcription factor activity

VMA00150

240615

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