NF Kappa B Inhibitor Alpha antibody | OTI1D4
Mouse anti NF Kappa B Inhibitor Alpha
NF kappa B inhibitor alpha antibody recognizes NF kappa B inhibitor alpha, also known as I-kappa-B-alpha, IkappaB alpha, IkB-alpha, or major histocompatibility complex enhancer-binding protein MAD3.
NF Kappa B Inhibitor Alpha Antibody with Control Lysate
NF kappa B inhibitor alpha antibody recognizes NF kappa B inhibitor alpha, also known as I-kappa-B-alpha, IkappaB alpha, IkB-alpha, or major histocompatibility complex enhancer-binding protein MAD3.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI1D4
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00150 | WB | 100 µl | ||||
| VMA00150KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human NF kappa B inhibitor alpha antibody recognizes NF kappa B inhibitor alpha, also known as I-kappa-B-alpha, IkappaBalpha, NF-kappa-B inhibitor alpha, ikB-alpha, major histocompatibility complex enhancer-binding protein MAD3 and nuclear factor of kappa light chain gene enhancer in B-cells.
Human NF kappa B inhibitor alpha is a member of the NF kappa B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF kappa B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in the NFKBIA gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease (provided by RefSeq, Aug 2011).
Mouse anti Human Human NF kappa B inhibitor alpha antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Human NF kappa B inhibitor alpha is a member of the NF kappa B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF kappa B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in the NFKBIA gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease (provided by RefSeq, Aug 2011).
Mouse anti Human Human NF kappa B inhibitor alpha antibody detects a band of 35 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti NF kappa B inhibitor alpha detects a band of approximately 35 kDa in MCF7 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human NF kappa B inhibitor alpha (NP_065390) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg MCF7 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P25963 Related reagents
- Entrez Gene
- NFKBIA Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006916 anti-apoptosis
- GO:0007253 cytoplasmic sequestering of NF-kappaB
- GO:0008063 Toll signaling pathway
- GO:0008139 nuclear localization sequence binding
- GO:0042802 identical protein binding
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010745 negative regulation of macrophage derived foam cell differentiation
- GO:0010875 positive regulation of cholesterol efflux
- GO:0010888 negative regulation of lipid storage
- GO:0031625 ubiquitin protein ligase binding
- GO:0032088 negative regulation of NF-kappaB transcription factor activity
- GO:0032270 positive regulation of cellular protein metabolic process
- GO:0033256 I-kappaB/NF-kappaB complex
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050852 T cell receptor signaling pathway
- GO:0051059 NF-kappaB binding
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of NF Kappa B Inhibitor Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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