Myosin antibody | AB03/2F2

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Mouse anti Myosin Regulatory Light Chain

Product Type
PrecisionAb Monoclonal
Clone
AB03/2F2
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00790
Datasheet
WB
SDS
100 µl loader

Mouse anti myosin antibody recognizes myosin regulatory light polypeptide 9 (MYL9), also known as MLC-2C.

The myosin superfamily contains actin-based molecular motors which facilitate muscle contraction among other motility processes, such as cytoplasmic contractility and mechanosensing. Myosin proteins use ATP hydrolysis to generate contractile force, powering movement (Heissler et al. 2016). Specific myosins are involved with specific functions. MYL9 is a regulatory subunit of myosin, which can be phosphorylated at threonine 18 and serine 19 to regulate activity. MYL9 also plays a unique role in the nucleus, transcriptionally activating intercellular adhesion molecule 1 (ICAM1) (Nevitt et al. 2018). Myosin functions as a tumor suppressor, with dysregulated activity in cancer. Loss of myosin has been shown to impact the metastatic potential of cells. However, targeting myosin may be difficult in cancer treatment due to its essential contractile functions (Surcel and Robinson 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti myosin antibody detects a band of approximately 18 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-172 of human myosin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P24844
Entrez Gene
MYL9
GO Terms
GO:0005509 calcium ion binding
GO:0003774 motor activity
GO:0006936 muscle contraction
GO:0005829 cytosol
GO:0005859 muscle myosin complex
GO:0006937 regulation of muscle contraction
GO:0007411 axon guidance
GO:0008307 structural constituent of muscle
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Myosin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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