LIN28B antibody | CD03/4D4

100% Secure


Mouse anti LIN28B

Product Type
PrecisionAb Monoclonal
Clone
CD03/4D4
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00864
Datasheet
WB
SDS
100 µl loader

Mouse anti LIN28B antibody recognizes protein lin-28 homolog B, also known as CSDD2.

LIN28B is a cytoplasmic RNA-binding protein (RBP) involved with orchestrating fetal hematopoiesis, able to specify the fate of hematopoietic stem and progenitor cells (HSPCs) (Wang et al. 2019). It is therefore highly expressed in embryonic tissues. LIN28B is completely silenced during differentiation and in healthy adult tissues, but is reactivated in numerous cancers - it is an oncofetal protein. Its expression has been associated with advanced disease and poor prognosis (Kugel et al. 2016). During pregnancy, decreased expression of LIN28B has been suggested to play a role in preeclampsia via reduction in trophoblast invasion and syncytialization and by promoting inflammation, hindering differentiation and migration of trophoblasts (Canfield et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti LIN28B antibody detects a band of approximately 24 and 33 kDa in HepG2 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-250 of human LIN28B
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q6ZN17
Entrez Gene
LIN28B
GO Terms
GO:0005515 protein binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006355 regulation of transcription, DNA-dependent
GO:0008270 zinc ion binding
GO:0010587 miRNA catabolic process
GO:0031054 pre-microRNA processing
GO:0031123 RNA 3'-end processing
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of LIN28B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra