LIN28B antibody | CD03/4D4
Mouse anti LIN28B antibody recognizes protein lin-28 homolog B, also known as CSDD2.
LIN28B is a cytoplasmic RNA-binding protein (RBP) involved with orchestrating fetal hematopoiesis, able to specify the fate of hematopoietic stem and progenitor cells (HSPCs) (Wang et al. 2019). It is therefore highly expressed in embryonic tissues. LIN28B is completely silenced during differentiation and in healthy adult tissues, but is reactivated in numerous cancers - it is an oncofetal protein. Its expression has been associated with advanced disease and poor prognosis (Kugel et al. 2016). During pregnancy, decreased expression of LIN28B has been suggested to play a role in preeclampsia via reduction in trophoblast invasion and syncytialization and by promoting inflammation, hindering differentiation and migration of trophoblasts (Canfield et al. 2019).
LIN28B is a cytoplasmic RNA-binding protein (RBP) involved with orchestrating fetal hematopoiesis, able to specify the fate of hematopoietic stem and progenitor cells (HSPCs) (Wang et al. 2019). It is therefore highly expressed in embryonic tissues. LIN28B is completely silenced during differentiation and in healthy adult tissues, but is reactivated in numerous cancers - it is an oncofetal protein. Its expression has been associated with advanced disease and poor prognosis (Kugel et al. 2016). During pregnancy, decreased expression of LIN28B has been suggested to play a role in preeclampsia via reduction in trophoblast invasion and syncytialization and by promoting inflammation, hindering differentiation and migration of trophoblasts (Canfield et al. 2019).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti LIN28B antibody detects a band of approximately 24 and 33 kDa in HepG2 cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-250 of human LIN28B
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q6ZN17
- Entrez Gene
- LIN28B
- GO Terms
- GO:0005515 protein binding
- GO:0003677 DNA binding
- GO:0003723 RNA binding
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006355 regulation of transcription, DNA-dependent
- GO:0008270 zinc ion binding
- GO:0010587 miRNA catabolic process
- GO:0031054 pre-microRNA processing
- GO:0031123 RNA 3'-end processing
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of LIN28B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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