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Ki67 antibody | RM360

Rabbit anti Ki67

Product Type
Monoclonal Antibody
Clone
RM360
Isotype
IgG
Specificity
Ki67

Product Code Applications Pack Size List Price Your Price Qty
MCA6357
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P 0.1 ml loader
List Price Your Price
loader

Rabbit anti Ki67 antibody recognizes proliferation marker protein Ki-67, also known as MKI67.

Ki67 is a nuclear marker which exists in proliferating cells, with levels decaying steadily in quiescent cells. It is a cell cycle protein present during G1, S, and G2 phases, and its transcription is under the control of E2F activation. Ki67 has been shown to participate in ribosomal biogenesis, heterochromatin organization, and mitotic chromosome separation (Miller et al. 2018). Ki67 is a widely-used indicator of proliferation in oncology across numerous cancer types. In breast cancer, Ki67 may be an indicator of responsiveness or resistance to chemotherapy or endocrine therapy (Niazi et al. 2018).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to the internal region of human Ki67
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Ki67 antibody

  1. Westhoff, M.A. et al. (2013) Sequential dosing in chemosensitization: targeting the PI3K/Akt/mTOR pathway in neuroblastoma.
    PLoS One. 8 (12): e83128.
  2. Fukase, M. et al. (2021) Intravenous injection of human multilineage-differentiating stress-enduring cells alleviates mouse severe acute pancreatitis without immunosuppressants
    Surgery Today. 52 (4): 603-15.

UniProt
P46013
Entrez Gene
MKI67
GO Terms
GO:0005524 ATP binding
GO:0005730 nucleolus
GO:0008022 protein C-terminus binding
GO:0008283 cell proliferation

MCA6357

157620 166691

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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